BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0035.p (640 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbasp... 627 0.0 gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbasp... 182 9e-47 gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbasp... 128 2e-30 gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbasp... 125 8e-30 gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPas... 70 5e-13 >gi|300310722|ref|YP_003774814.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 640 Score = 627 bits (1618), Expect = 0.0, Method: Compositional matrix adjust. Identities = 298/298 (100%), Positives = 298/298 (100%) Query: 1 MAVISLSDAQLAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLL 60 MAVISLSDAQLAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLL Sbjct: 1 MAVISLSDAQLAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLL 60 Query: 61 VMQQNLKIAYVDQEPHFPEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERM 120 VMQQNLKIAYVDQEPHFPEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERM Sbjct: 61 VMQQNLKIAYVDQEPHFPEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERM 120 Query: 121 HQIQTVLDAADAWSIGNKVETTLDKLNLNRDARIGELSGGMKKRVALACGLVGNPDVLLL 180 HQIQTVLDAADAWSIGNKVETTLDKLNLNRDARIGELSGGMKKRVALACGLVGNPDVLLL Sbjct: 121 HQIQTVLDAADAWSIGNKVETTLDKLNLNRDARIGELSGGMKKRVALACGLVGNPDVLLL 180 Query: 181 DEPTNHLDFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTT 240 DEPTNHLDFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTT Sbjct: 181 DEPTNHLDFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTT 240 Query: 241 YQTRKAELLANEEVENAKFDKFLAQEEVWIRKGVQARRTRDEGRVRRLEALRVQRSER 298 YQTRKAELLANEEVENAKFDKFLAQEEVWIRKGVQARRTRDEGRVRRLEALRVQRSER Sbjct: 241 YQTRKAELLANEEVENAKFDKFLAQEEVWIRKGVQARRTRDEGRVRRLEALRVQRSER 298 Score = 85.9 bits (211), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 73/225 (32%), Positives = 111/225 (49%), Gaps = 38/225 (16%) Query: 3 VISLSDAQLAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVM 62 V L + A+G ++ L G+++GLIG+NG GK++LLK+I G D G + Sbjct: 319 VAELENVNKAYGPKTIVRDFSTILMRGDKVGLIGQNGAGKTTLLKLILGEEAPDSGTVKQ 378 Query: 63 QQNLKIAYVDQ-EPHFPEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERMH 121 +++AY DQ EE T+ D +A G +++ + GQ R H Sbjct: 379 GTKMQVAYFDQMRAQLNEETTLADTIAPG--------SDWVEVNGQ-----------RKH 419 Query: 122 QIQTVLDAADAWSIGNKVETTLDKLNLNRDAR--IGELSGGMKKRVALACGLVGNPDVLL 179 + T L D L AR + LSGG + R+ LA +VL+ Sbjct: 420 -VMTYLS---------------DFLFAPERARSPVRTLSGGERNRLLLARLFAKPANVLV 463 Query: 180 LDEPTNHLDFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRII 224 LDEPTN LD ++ LE LL +++G++ ++HDR FLDNV T++I Sbjct: 464 LDEPTNDLDIDTLELLEELLEEYEGTVFLVSHDRMFLDNVVTQVI 508 >gi|300313989|ref|YP_003778081.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 555 Score = 182 bits (461), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 111/277 (40%), Positives = 164/277 (59%), Gaps = 3/277 (1%) Query: 18 LLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVMQQNLKIAYVDQEPHF 77 +L S G +IG++G NG+GKS+LLKI+AG K +G + NL I Y+ QEP Sbjct: 21 ILKDISLSFFPGAKIGVLGLNGSGKSTLLKIMAGIDKEIEGEAIPMPNLNIGYLPQEPQL 80 Query: 78 PEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDD--ALLERMHQIQTVLDAADAWSI 135 E TV V S LGE+ A+ +A+ + ++ D AL +++ ++ AAD S+ Sbjct: 81 DAEKTVRQEVESALGEVFEAQAKLDAVYAAYAEEDADFDALATEQARLEAIIAAADGNSL 140 Query: 136 GNKVETTLDKLNLNR-DARIGELSGGMKKRVALACGLVGNPDVLLLDEPTNHLDFGSILW 194 ++E D L L DA+IG LSGG K+RVAL L+ PD+LLLDEPTNHLD S+ W Sbjct: 141 NQQLEMAADALRLPPWDAKIGVLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVDW 200 Query: 195 LEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTTYQTRKAELLANEEV 254 LE L+ F G+++ ITHDR FLDN + I+ELDRG + + GN++++ +K L EE Sbjct: 201 LEQFLQRFPGTVVAITHDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEARLKQEEA 260 Query: 255 ENAKFDKFLAQEEVWIRKGVQARRTRDEGRVRRLEAL 291 + K +A+E W+R+ + R+ + + R+ R L Sbjct: 261 SESARQKTIAKELEWVRQNPKGRQAKSKARLARFNEL 297 Score = 115 bits (287), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 82/259 (31%), Positives = 124/259 (47%), Gaps = 49/259 (18%) Query: 3 VISLSDAQLAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVM 62 VI + +G L+D F + G +G+IG NG GKS+L +++AGR D G +V+ Sbjct: 323 VIEFKNVSKGYGDRLLIDDLNFKIPAGAIVGIIGPNGAGKSTLFRMLAGRETPDSGEVVL 382 Query: 63 QQNLKIAYVDQE-PHFPEEMTVFDAVASGLGELPALLAEYE----AITGQF---GGDNDD 114 +K++ VDQ + TVF+ V+ G L + +E A G+F GGD Sbjct: 383 GPTVKVSLVDQSREDLGNKKTVFEDVSGGADILT--VGRFEMPSRAYLGRFNFKGGDQ-- 438 Query: 115 ALLERMHQIQTVLDAADAWSIGNKVETTLDKLNLNRDARIGELSGGMKKRVALACGLVGN 174 Q + +G LSGG + R+ LA L+ Sbjct: 439 ---------QKI---------------------------VGNLSGGERGRLHLAKTLLQG 462 Query: 175 PDVLLLDEPTNHLDFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELD-RGKILS 233 +VLLLDEP+N LD ++ LE L +F G++L I+HDR FLD ++T II + ++ Sbjct: 463 GNVLLLDEPSNDLDVETLRALEDALLEFAGTVLVISHDRWFLDRIATHIIAFEGDSQVTF 522 Query: 234 FPGNFTTYQTRKAELLANE 252 F GN+ Y+ K + L E Sbjct: 523 FDGNYQEYEADKKKRLGEE 541 >gi|300311473|ref|YP_003775565.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 128 bits (321), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 83/239 (34%), Positives = 132/239 (55%), Gaps = 10/239 (4%) Query: 18 LLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVMQQNLKIAYVDQEPHF 77 LL++ + +L G++IGLIG NG GKSSL ++ G D G + ++AYV QE Sbjct: 16 LLENVDLTLNPGDKIGLIGANGAGKSSLFAMLRGELHADQGNIDFPARWRMAYVAQETPA 75 Query: 78 PEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERMHQIQTVLDAADAWSIGN 137 + + A+ G L L AE Q +D + +H T L ADA+++ + Sbjct: 76 LDRPAIEYAI-DGDAHLRQLEAELARAEAQPDDKHDGNHIAELH---TALADADAYTVRS 131 Query: 138 KVETTLDKLNLNRDAR---IGELSGGMKKRVALACGLVGNPDVLLLDEPTNHLDFGSILW 194 + E L L + + + SGG + R+ LA L+ D+LLLDEPTNHLD +I+W Sbjct: 132 RAEQLLLGLGFSLEQMERPVASFSGGWRMRLNLAQALMCPSDLLLLDEPTNHLDLDAIIW 191 Query: 195 LEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTTYQTRKA---ELLA 250 LE L+ ++G++L I+HDR FLD V I+ +D K+ + GN++ ++ +++ EL+A Sbjct: 192 LEDWLKRYEGTLLIISHDRDFLDGVVNVIVHIDDRKLKRYSGNYSAFERQRSAQLELMA 250 Score = 69.3 bits (168), Expect = 8e-13, Method: Compositional matrix adjust. Identities = 64/232 (27%), Positives = 99/232 (42%), Gaps = 59/232 (25%) Query: 23 EFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVMQQNLKIAYVDQEPHFPEEMT 82 +FSL+ G+RIGL+G NG AG+S L +K + +P Sbjct: 346 DFSLQNGQRIGLLGVNG----------AGKSTL----------IKTVAGEIDP------- 378 Query: 83 VFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERMHQIQTV-LDAADAWSI------ 135 SG L L + G F HQ++ + D + W + Sbjct: 379 -----LSGTARLGKGL-----VIGYFA----------QHQVEMLRHDESPLWHLTRLAPD 418 Query: 136 --GNKVETTLDKLNLN---RDARIGELSGGMKKRVALACGLVGNPDVLLLDEPTNHLDFG 190 ++ L N N + I SGG K R+ALA + P++LLLDEPTNHLD Sbjct: 419 VREQELRNFLGSFNFNGTMATSSIAPFSGGEKARLALALIVWQRPNLLLLDEPTNHLDLE 478 Query: 191 SILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTTYQ 242 + L L F+G+++ ++HDR L + + + + GK+ F G+ Y+ Sbjct: 479 TREALTMALAQFEGTLVLVSHDRHLLRATTDQFLIVAEGKLQPFDGDLDDYK 530 >gi|300311530|ref|YP_003775622.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 532 Score = 125 bits (315), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 89/277 (32%), Positives = 142/277 (51%), Gaps = 13/277 (4%) Query: 3 VISLSDAQLAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDGLLVM 62 ++S ++ + FG L ++ G R GLIG NG GKS+ +KI+ G + G + + Sbjct: 1 MLSTANITMQFGAKPLFENISVKFGDGNRYGLIGANGCGKSTFMKILGGDLEPSAGNVSL 60 Query: 63 QQNLKIAYVDQEPHFPEEMTVFDAVASGLGELPALLAEYEAITGQFGGDNDDALLERMHQ 122 N ++ + Q+ EEM V D V G E+ A ++E +AI +DD + Sbjct: 61 DVNERLGKLRQDQFAYEEMRVLDVVMMGHVEMWAAMSERDAIYANPDATDDD------YM 114 Query: 123 IQTVLDAADAWSIGNKVETTLDKLNLNRDARIGELSGGM-------KKRVALACGLVGNP 175 L+A A G E +L L I + +G M K RV LA L NP Sbjct: 115 KAADLEAKFAEYDGYTAEARAGELLLGVGVPIEQHNGPMSNVAPGWKLRVLLAQALFSNP 174 Query: 176 DVLLLDEPTNHLDFGSILWLEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFP 235 D+LLLDEPTN+LD +I WLE +L + +++ I+HDR FL+ V T + ++D G + +P Sbjct: 175 DILLLDEPTNNLDINTIRWLEEVLNERNSTMIIISHDRHFLNQVCTHMADMDYGTLKIYP 234 Query: 236 GNFTTYQTRKAELLANEEVENAKFDKFLAQEEVWIRK 272 GN+ Y ++ A + NAK + +A+ + ++R+ Sbjct: 235 GNYDDYMLASSQARAQQLAANAKAKEKVAELQDFVRR 271 Score = 96.7 bits (239), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 74/227 (32%), Positives = 113/227 (49%), Gaps = 53/227 (23%) Query: 26 LETGERIGLIGRNGTGKSSLLKIIAGR-SKL--DDGLLVMQQNLKIAYVDQEPHFPEEMT 82 +E GE+I +IG NG GK++LL+ I G + L D GL+ +N + Y+ Q+P EE Sbjct: 341 VEAGEKIAIIGANGAGKTTLLRSIGGDVTGLHPDGGLVKWAENANVGYMPQDP--TEEF- 397 Query: 83 VFDAVASGLGELPALLAEYEAITGQFG-----GDNDDA---LLERMHQIQTVLDAADAWS 134 A G E +T G GD+D A +L R+ L + D Sbjct: 398 -----AGG-----------ETLTDWMGKWTQEGDDDQAVRGILGRL------LFSGDDVK 435 Query: 135 IGNKVETTLDKLNLNRDARIGELSGGMKKRVALACGLVGNPDVLLLDEPTNHLDFGSILW 194 KV LSGG K R+ ++G +VLL+DEPTNH+D SI Sbjct: 436 KSVKV-----------------LSGGEKGRMMYGKLMLGRHNVLLMDEPTNHMDMESIES 478 Query: 195 LEGLLRDFKGSILFITHDRSFLDNVSTRIIELDRGKILSFPGNFTTY 241 L L + G+++F++HDR F+ +++TRI+E+ G+++ F G + Y Sbjct: 479 LNIALEKYAGTLIFVSHDREFVSSLATRILEVKDGQVIDFQGGYEDY 525 >gi|300311157|ref|YP_003775249.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 923 Score = 70.1 bits (170), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 62/190 (32%), Positives = 91/190 (47%), Gaps = 26/190 (13%) Query: 3 VISLSDAQLAFGHVALLDHAEFSLETGERIGLIGRNGTGKSSLLKIIAGRSKLDDG-LLV 61 V L L +G LD + G +GLIG +G GKSSLL ++AG L G L V Sbjct: 10 VARLQRVALRYGKTVALDAVSLDIPAGCMVGLIGPDGVGKSSLLSLVAGARALQQGELAV 69 Query: 62 MQQNLKIAYVDQEPHFPEEMTVFDAVASGLGE--LPAL-LAEYEAITGQFGGDNDDALLE 118 + +++ Q+ H + + GLG+ P L + E G+ G + Sbjct: 70 LGGDMR-----QQRHRQQACPRIAYMPQGLGKNLYPTLSVEENLQFFGRLFGQDASERRR 124 Query: 119 RMHQIQTVLDAADAWSIGNKVETTLDKLNLNRDARIGELSGGMKKRVALACGLVGNPDVL 178 R+ ++ T T LDK L R A G+LSGGMK+++ L C L+ +PD+L Sbjct: 125 RIDELTT--------------STGLDKF-LARPA--GKLSGGMKQKLGLCCSLIHDPDLL 167 Query: 179 LLDEPTNHLD 188 +LDEPT +D Sbjct: 168 ILDEPTTGVD 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.137 0.472 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,496,154 Number of extensions: 54379 Number of successful extensions: 579 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 573 Number of HSP's successfully gapped: 43 Length of query: 640 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 549 Effective length of database: 1,188,985 Effective search space: 652752765 Effective search space used: 652752765 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 134 (56.2 bits)