BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0030.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310718|ref|YP_003774810.1| phosphate regulon two-component... 466 e-133 gi|300310592|ref|YP_003774684.1| phosphate regulon two-component... 255 3e-69 gi|300310849|ref|YP_003774941.1| two component transcription reg... 147 9e-37 gi|300309361|ref|YP_003773453.1| response regulator for cobalt/z... 137 7e-34 gi|300313896|ref|YP_003777988.1| two component transcription res... 137 7e-34 >gi|300310718|ref|YP_003774810.1| phosphate regulon two-component response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 466 bits (1200), Expect = e-133, Method: Compositional matrix adjust. Identities = 230/230 (100%), Positives = 230/230 (100%) Query: 1 MSKELPHILMVEDEPSIAELLRFTMQGAGFATTIATDVAQARKHIAESLPHVVLLDWMLP 60 MSKELPHILMVEDEPSIAELLRFTMQGAGFATTIATDVAQARKHIAESLPHVVLLDWMLP Sbjct: 1 MSKELPHILMVEDEPSIAELLRFTMQGAGFATTIATDVAQARKHIAESLPHVVLLDWMLP 60 Query: 61 DSSGLVLLNELRRDQRTASLPVIMLTAKGMEEDKVKGLNEGADDYVTKPFSPKELVARVQ 120 DSSGLVLLNELRRDQRTASLPVIMLTAKGMEEDKVKGLNEGADDYVTKPFSPKELVARVQ Sbjct: 61 DSSGLVLLNELRRDQRTASLPVIMLTAKGMEEDKVKGLNEGADDYVTKPFSPKELVARVQ 120 Query: 121 ALLRRKAPEIGSQALKYRDISIDVERRQVSVADKPVLLDQAEFKLLRYLVAHPERIFSRA 180 ALLRRKAPEIGSQALKYRDISIDVERRQVSVADKPVLLDQAEFKLLRYLVAHPERIFSRA Sbjct: 121 ALLRRKAPEIGSQALKYRDISIDVERRQVSVADKPVLLDQAEFKLLRYLVAHPERIFSRA 180 Query: 181 QLLDKVWGDHTFIEERTVDVHIMRLRKSLGDLAHYVQTVRGMGYKLSAEQ 230 QLLDKVWGDHTFIEERTVDVHIMRLRKSLGDLAHYVQTVRGMGYKLSAEQ Sbjct: 181 QLLDKVWGDHTFIEERTVDVHIMRLRKSLGDLAHYVQTVRGMGYKLSAEQ 230 >gi|300310592|ref|YP_003774684.1| phosphate regulon two-component response regulator transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 245 Score = 255 bits (651), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 120/222 (54%), Positives = 167/222 (75%) Query: 8 ILMVEDEPSIAELLRFTMQGAGFATTIATDVAQARKHIAESLPHVVLLDWMLPDSSGLVL 67 IL+VEDEPSIAEL+ FT++ AG+++ + ++ A A + P +VLLDWMLPD SGL L Sbjct: 24 ILIVEDEPSIAELIAFTLKEAGWSSFVVSNAAAAWDFVQHRAPQLVLLDWMLPDRSGLHL 83 Query: 68 LNELRRDQRTASLPVIMLTAKGMEEDKVKGLNEGADDYVTKPFSPKELVARVQALLRRKA 127 L+ +R D++ L VIMLTAK MEEDK+ GL+ GADDY+TKPFSP+EL AR++ALLRRK+ Sbjct: 84 LSRIRADRQLQQLSVIMLTAKSMEEDKIAGLDNGADDYITKPFSPRELTARIKALLRRKS 143 Query: 128 PEIGSQALKYRDISIDVERRQVSVADKPVLLDQAEFKLLRYLVAHPERIFSRAQLLDKVW 187 PE + + I++D VS++ + + AE+KLL++ +AHP+R+FSR+QLLD+VW Sbjct: 144 PEHAQEVMSAGPITLDPASCSVSLSGARIDIGHAEYKLLKFFLAHPDRVFSRSQLLDRVW 203 Query: 188 GDHTFIEERTVDVHIMRLRKSLGDLAHYVQTVRGMGYKLSAE 229 GDH IEERTVDVH++RLRK+L D + ++TVR +GY LS + Sbjct: 204 GDHVVIEERTVDVHVLRLRKALRDAEYLIKTVRSVGYMLSTK 245 >gi|300310849|ref|YP_003774941.1| two component transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 248 Score = 147 bits (370), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 90/232 (38%), Positives = 136/232 (58%), Gaps = 13/232 (5%) Query: 7 HILMVEDEPSIAELLRFTMQGAGFATTIATDVAQARKHIAESLPHVVLLDWMLPDSSGLV 66 HIL+V+D+ I LL + G T AT+ ++ R+ + ES +++LD LP GL Sbjct: 9 HILVVDDDRDIRTLLAEYLDSNGLRTLTATNGSEMRRVLEESRVDLIVLDLTLPGEDGLT 68 Query: 67 LLNELRRDQRTASLPVIMLTAKGMEEDKVKGLNEGADDYVTKPFSPKELVARVQALLRRK 126 L LR T+S+PVIMLTA+G D++ GL GADDY+ KPF P+EL AR++++LRR Sbjct: 69 LCRNLRA---TSSVPVIMLTARGEPLDRILGLEMGADDYLAKPFEPRELFARIRSVLRRT 125 Query: 127 ---APEIGS---QALKYRDISIDVERRQVSVADKPVL-LDQAEFKLLRYLVAHPERIFSR 179 P + A+++ ++D+ R + D V+ L AEF+LL+ + HP R+ +R Sbjct: 126 QALPPNMAQPDVAAMRFAGWTLDLTARHLVNRDGVVVALSGAEFRLLKVFLDHPNRVLNR 185 Query: 180 AQLLDKVWGDHTFIEERTVDVHIMRLRKSLGDLAH---YVQTVRGMGYKLSA 228 QLL+ G + +R+VD+ I RLR+ LGD A ++TVR GY L+ Sbjct: 186 DQLLELTQGRESDPFDRSVDIQISRLRQKLGDDARTPTIIKTVRNEGYVLAT 237 >gi|300309361|ref|YP_003773453.1| response regulator for cobalt/zinc/cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 235 Score = 137 bits (345), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 92/222 (41%), Positives = 127/222 (57%), Gaps = 10/222 (4%) Query: 7 HILMVEDEPSIAELLRFTMQGAGFATT-IATDVAQARKHIAESLPH-VVLLDWMLPDSSG 64 HIL+VEDEP + LR + +GF + T V H A +L + +++LD MLP +G Sbjct: 2 HILLVEDEPKSGDYLRRGLTESGFVVDWVQTGVDGL--HNARTLDYKLIILDVMLPGMNG 59 Query: 65 LVLLNELRRDQRTASLPVIMLTAKGMEEDKVKGLNEGADDYVTKPFSPKELVARVQALLR 124 +L ELRR T PV+ LTA+ ED+VKGL GADDY+ KPF+ EL+ARV+ LLR Sbjct: 60 WDILRELRR---THDTPVLFLTARDEVEDRVKGLELGADDYLVKPFAYTELLARVRTLLR 116 Query: 125 RKAPEIGSQALKYRDISIDVERRQVSVADKPVLLDQAEFKLLRYLVAHPERIFSRAQLLD 184 R P ++Y D+ +DV RR+V+ A + + L EF LL L+ P + SR Q+ Sbjct: 117 R-GPSREVDLIEYDDVVLDVLRRKVTRAGQRIDLTAKEFALLHMLIRRPGEVLSRPQIAS 175 Query: 185 KVWGDHTFIEERTVDVHIMRLRKSL--GDLAHYVQTVRGMGY 224 +VW + + VDV + RLR L G + TV GMGY Sbjct: 176 QVWDMNFDSDTNVVDVAVRRLRAKLDEGFEKKLLHTVWGMGY 217 >gi|300313896|ref|YP_003777988.1| two component transcription response regulator protein [Herbaspirillum seropedicae SmR1] Length = 244 Score = 137 bits (345), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 87/223 (39%), Positives = 129/223 (57%), Gaps = 9/223 (4%) Query: 8 ILMVEDEPSIAELLRFTMQGAGFATTIATDVAQARKHIAESLPHVVLLDWMLPDSSGLVL 67 IL+VEDEP +AELL+ + AG++ A + P ++LLD MLP G + Sbjct: 18 ILIVEDEPKLAELLQKYLALAGYSARHVARGDAALDAVRAQRPDLILLDIMLPGMDGWEI 77 Query: 68 LNELRRDQRTASLPVIMLTAKGMEEDKVKGLNEGADDYVTK-PFSPKELVARVQALLRRK 126 +LR + +PV+MLTA+ EED+++GL GADDY+ K PFSP+E+VARV+++LRR Sbjct: 78 CRQLRT---FSDVPVLMLTARAEEEDRLRGLELGADDYIGKTPFSPREIVARVKSMLRRN 134 Query: 127 A--PEIGSQALKYRDISIDVERRQVSVADKPVLLDQAEFKLLRYLVAHPERIFSRAQLLD 184 A + +QA + ID R Q SV + + L E +LL+ + P ++FSR QLL+ Sbjct: 135 ALVQQRTAQAPARSLLHIDDVRHQASVRGEALNLTPLELRLLKTFASAPGQVFSREQLLN 194 Query: 185 KVWGDHTFIEERTVDVHIMRLRKSLGDL---AHYVQTVRGMGY 224 + D + +R VD HI LR+ L + +Q V G+GY Sbjct: 195 HLHDDDRSVTDRAVDTHIKNLRRKLEPCFPGHNAIQAVYGVGY 237 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 675,347 Number of extensions: 26534 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 183 Number of HSP's successfully gapped: 47 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)