BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0007.0001.p (438 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 857 0.0 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 499 e-142 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 396 e-111 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 337 1e-93 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 229 5e-61 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 857 bits (2215), Expect = 0.0, Method: Compositional matrix adjust. Identities = 434/438 (99%), Positives = 435/438 (99%) Query: 1 VADIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSE 60 VADIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSE Sbjct: 61 VADIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSE 120 Query: 61 TASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQ 120 TASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQ Sbjct: 121 TASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQ 180 Query: 121 ISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVE 180 ISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVE Sbjct: 181 ISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVE 240 Query: 181 SARVDVESYTAQVDQDRNALVLLVGKDLPAALLPQGLPDTRLAAASPLATIPPGLPSDLL 240 SARVDVESYTAQVDQDRNALVLLVGKDLP ALLPQGLPDTRLAAASPLATIPPGLPSDLL Sbjct: 241 SARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSDLL 300 Query: 241 QRRADIVQAERDLRAANANIGAARAAFYPSITLTANAGTASARLAGLFKGGSGSWSFVPQ 300 QRRADIVQAERDLRAANANIGAARAAFYPSITLT NAGTASARLAGLFKGGSGSWSFVPQ Sbjct: 301 QRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVPQ 360 Query: 301 ISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEAL 360 ISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEAL Sbjct: 361 ISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEAL 420 Query: 361 VQASDEAFRLSQARYNSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGG 420 VQASDEAFRLSQARY+SGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGG Sbjct: 421 VQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGG 480 Query: 421 WVERTGTPVASDIATQEP 438 WVERTGTP ASDIATQEP Sbjct: 481 WVERTGTPAASDIATQEP 498 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 499 bits (1285), Expect = e-142, Method: Compositional matrix adjust. Identities = 259/422 (61%), Positives = 320/422 (75%), Gaps = 4/422 (0%) Query: 4 IGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETAS 63 +GWR+ F DP LQQVIA AL+NNRDLRVA LNIEKARAQY + A +PSI A + +AS Sbjct: 71 LGWREFFLDPRLQQVIAAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSAS 130 Query: 64 RTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISL 123 RTP +LS+TGSA S + T+G SAYELD FGR+++L + AL+S+ E RRST ISL Sbjct: 131 RTPAELSTTGSAAVSHQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISL 190 Query: 124 IAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESAR 183 +AEVATAWLTLASD+D L+LAQDT SQS ++AL+QRS ++G+ S + L + +VESAR Sbjct: 191 VAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESAR 250 Query: 184 VDVESYTAQVDQDRNALVLLVGKDLPAALLPQGLPDTRLAAASPLATIPPGLPSDLLQRR 243 DV Y +QV+QD NAL LLVG D+ AALLP GLPDT AS L P GLPS +L RR Sbjct: 251 ADVARYASQVEQDLNALRLLVGADIDAALLPAGLPDT----ASVLPATPAGLPSQVLLRR 306 Query: 244 ADIVQAERDLRAANANIGAARAAFYPSITLTANAGTASARLAGLFKGGSGSWSFVPQISL 303 D++ AE L++ANA+IGAARAAF+PSI+LTAN GTAS L GLFK GSGSW+F P I+L Sbjct: 307 PDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINL 366 Query: 304 PIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQA 363 PIF+ G+ +A+LD A ++RDI VA YEKAIQTAFREV+DALA R TL ++L AQ A Sbjct: 367 PIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAA 426 Query: 364 SDEAFRLSQARYNSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGGWVE 423 S++AFRLS ARY +GVDSYLT L +QRTLYSAQ+ LI +L+ +N +T YK LGGGW E Sbjct: 427 SEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGGGWSE 486 Query: 424 RT 425 +T Sbjct: 487 QT 488 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 396 bits (1018), Expect = e-111, Method: Compositional matrix adjust. Identities = 223/426 (52%), Positives = 284/426 (66%), Gaps = 11/426 (2%) Query: 3 DIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETA 62 ++GWR F D LQQ+IA ALENNRDLR AVLNIE+ARAQY + RA L P++ + + Sbjct: 59 NLGWRQFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAAR 118 Query: 63 SRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQIS 122 +RT S TG S SY L AYELD FGRVR+LK+ AL S+LST EA++S QIS Sbjct: 119 TRTAASQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQIS 178 Query: 123 LIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESA 182 L+A+VA A+L S ++L+LA +TL+S+ S+ L Q+ ++G+S+AL R QT VESA Sbjct: 179 LVAQVAQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESA 238 Query: 183 RVDVESYTAQVDQDRNALVLLVGK---DLPAALLPQGLPDTRLAAASPLATIPPGLPSDL 239 RV Q Q NAL LLVGK +LP A L L D ++ + IP GLPSDL Sbjct: 239 RVAKTQLERQRAQAENALTLLVGKKIGELPPAQL---LSDEKI-----VTDIPAGLPSDL 290 Query: 240 LQRRADIVQAERDLRAANANIGAARAAFYPSITLTANAGTASARLAGLFKGGSGSWSFVP 299 L R DI AE+ L AANANIGAARAAF+P I+LT N G+AS+ L GLF+ GSG+WSF P Sbjct: 291 LTNRPDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGP 350 Query: 300 QISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEA 359 +SLPIFD G N ANLD+A V ++ +V YEKAIQTAFREV+DAL R +L Q++AQEA Sbjct: 351 SLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEA 410 Query: 360 LVQASDEAFRLSQARYNSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGG 419 + A E +L+ R+ +G+ S L LD+QR L+SAQ+ LI + R N + YK LGG Sbjct: 411 FLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 Query: 420 GWVERT 425 G E T Sbjct: 471 GLNENT 476 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 337 bits (864), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 194/416 (46%), Positives = 265/416 (63%), Gaps = 5/416 (1%) Query: 4 IGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETAS 63 + WRD FTDP LQ +I ALEN+RDLR A L +++A+A YG+ A LFP+I A S Sbjct: 64 LPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRS 123 Query: 64 RTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISL 123 RTP DL+ T L +Y A LG S++E+DL+GR+RSL + AL+S ++ R + +SL Sbjct: 124 RTPADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSL 183 Query: 124 IAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESAR 183 +A+VA AWL LA +RL +A T+ S+ S+ + R E+G++S L L Q +T + A+ Sbjct: 184 MAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQ 243 Query: 184 VDVESYTAQVDQDRNALVLLVGKDLPAALLPQGLPDTRLAAASPLATIPPGLPSDLLQRR 243 Q Q NAL LLVG + +LP G R + A + PGLP+ LL RR Sbjct: 244 ALGAQLQQQRAQRLNALTLLVGSPV---VLPVG--QARAGLDAVFAPLQPGLPALLLTRR 298 Query: 244 ADIVQAERDLRAANANIGAARAAFYPSITLTANAGTASARLAGLFKGGSGSWSFVPQISL 303 D+ AE L AANANIGAARAAF+PSI+LT N GTASA L+GLF+ GS +W+F P I+L Sbjct: 299 PDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITL 358 Query: 304 PIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQA 363 PIF G + NL ++ V RD++VA YEK +QTAFREV+DALA R L +QL+ ++ V+ Sbjct: 359 PIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRV 418 Query: 364 SDEAFRLSQARYNSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGG 419 E RLS RY++G +L VLD+QR L SAQ+ L+ + + ++ V Y LGG Sbjct: 419 QRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 229 bits (583), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 159/418 (38%), Positives = 230/418 (55%), Gaps = 7/418 (1%) Query: 4 IGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETAS 63 IGW+D F LQ +IA AL +N DLR L +++ +AQ+ +QR+ P++ S Sbjct: 57 IGWQDYFPQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRS 116 Query: 64 RTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISL 123 R P + +G A ++R Y LG S++ELD +GR+ SL E ALQ ++++ A ++ ISL Sbjct: 117 RGPLEPPESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISL 176 Query: 124 IAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESAR 183 I +VA AW +L +RL L + + + S AL Q + G + A L Q ++ + R Sbjct: 177 IGQVAAAWTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMR 236 Query: 184 VDVESYTAQVDQDRNALVLLVG-KDLPAALLPQGLPDTRLAAASPLATIPPGLPSDLLQR 242 + R+ L +L G LPAAL P P T L P A + PGLPS LL Sbjct: 237 AEQAELQQMRASQRSTLEMLAGLTQLPAALEPA--PTTILL---PFA-LAPGLPSTLLAA 290 Query: 243 RADIVQAERDLRAANANIGAARAAFYPSITLTANAGTASARLAGLFKGGSGSWSFVPQIS 302 R DI+ AE L+A+ A + AARAAF+P I+LTA G AS L LFKG S +W+FVP+IS Sbjct: 291 RPDIIAAEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEIS 350 Query: 303 LPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEALVQ 362 LP+ DGG Q+ DIA + + ++ YE+ +Q AFREV+ L + + L QL+ + +VQ Sbjct: 351 LPLLDGGLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQ 410 Query: 363 ASDEAFRLSQARYNSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGGG 420 + R G S L VL+S+R AQ+ + + N + Y LGGG Sbjct: 411 QQAQRQAQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGG 468 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.345 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,187,106 Number of extensions: 44341 Number of successful extensions: 300 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 250 Number of HSP's successfully gapped: 19 Length of query: 438 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 350 Effective length of database: 1,203,190 Effective search space: 421116500 Effective search space used: 421116500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)