BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0052.p (922 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 1841 0.0 gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 134 2e-32 gi|300313429|ref|YP_003777521.1| zinc protease-like signal pepti... 94 5e-20 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 1841 bits (4768), Expect = 0.0, Method: Compositional matrix adjust. Identities = 911/924 (98%), Positives = 913/924 (98%), Gaps = 2/924 (0%) Query: 1 LSLRCPRLAILVLSLGFFLSVPAAQAATADAAPPAA--IKSLPAGVSAGPSAEGISEYRF 58 LSLRCPRLA+LVLSLGFFLSVPAAQAATA A IKSLPAGVSAGPSAEGISEYRF Sbjct: 6 LSLRCPRLALLVLSLGFFLSVPAAQAATATADAAPPAAIKSLPAGVSAGPSAEGISEYRF 65 Query: 59 ANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPRHPSIPQDFS 118 ANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPRHPSIPQDFS Sbjct: 66 ANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPRHPSIPQDFS 125 Query: 119 KRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDLDSEMTVVRNEF 178 KRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDLDSEMTVVRNEF Sbjct: 126 KRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDLDSEMTVVRNEF 185 Query: 179 EAGETSPARVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFYRTYYQPDNAVLL 238 EAGETSP RVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFYRTYYQPDNAVLL Sbjct: 186 EAGETSPTRVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFYRTYYQPDNAVLL 245 Query: 239 IAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFWTVEPTQDGERQFVIRRRGDQQLVALAYK 298 IAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFWTVEPTQDGERQFVIRRRGDQQLVALAYK Sbjct: 246 IAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFWTVEPTQDGERQFVIRRRGDQQLVALAYK 305 Query: 299 MPSALHPDATALGFAADILTDTPNGRLHKALVETGMATEVYAMPLEGMAPGLQMMVAKVK 358 MPSALHPDATALGFAADILTDTPNGRLHKALVETGMATEVYAMPLEGMAPGLQMMVAKVK Sbjct: 306 MPSALHPDATALGFAADILTDTPNGRLHKALVETGMATEVYAMPLEGMAPGLQMMVAKVK 365 Query: 359 VGGDLDAVRQAMISAVESFSTQPPTPEEVARLHREAANSFETLQNNPQQLAVAMSNAIAR 418 VGGDLDAVRQAMISAVESFSTQPPTPEEVARLHREAANSFETLQNNPQQLAVAMSNAIAR Sbjct: 366 VGGDLDAVRQAMISAVESFSTQPPTPEEVARLHREAANSFETLQNNPQQLAVAMSNAIAR 425 Query: 419 GDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTVGLYLPDDHPQRAEIPTAPAIDTLL 478 GDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTVGLYLPDDHPQRAEIPTAPA+DTLL Sbjct: 426 GDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTVGLYLPDDHPQRAEIPTAPALDTLL 485 Query: 479 SQYQPRTAIASGEAFDPAPANIEQRTTRIAPGQPPHTALDLALLPKQSRGQTVSVSLHLR 538 SQYQPRTAIASGEAFDPAPANIEQRTTRIAPGQPPHTALDLALLPKQSRGQTVSVSLHLR Sbjct: 486 SQYQPRTAIASGEAFDPAPANIEQRTTRIAPGQPPHTALDLALLPKQSRGQTVSVSLHLR 545 Query: 539 FGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARLKINGSLYRFQTTRENLVPAI 598 FGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARLKINGSLYRFQTTRENLVPAI Sbjct: 546 FGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARLKINGSLYRFQTTRENLVPAI 605 Query: 599 ALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTRAGEALSVHFDHYPAGDWRAAQT 658 ALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTRAGEALSVHFDHYPAGDWRAAQT Sbjct: 606 ALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTRAGEALSVHFDHYPAGDWRAAQT 665 Query: 659 LEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFDPAQARSAIDQAFGGWTSAAPYA 718 LEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFDPAQARSAIDQAFGGWTSAAPYA Sbjct: 666 LEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFDPAQARSAIDQAFGGWTSAAPYA 725 Query: 719 RVLQTWADIPAKRLLVDTPDKENGVYFARQNIRLRDTDPDYPALAVANFLLGGSSLKSRL 778 RVLQTWADIPAKRLLVDTPDKENGVYFARQNIRL DTDPDYPALAVANFLLGGSSLKSRL Sbjct: 726 RVLQTWADIPAKRLLVDTPDKENGVYFARQNIRLSDTDPDYPALAVANFLLGGSSLKSRL 785 Query: 779 ADRVRQQDGLSYGIYSGLQVAALTDAGHLTIYAIAAPQNLDKVDAAVREEIALALKDGFS 838 ADRVRQQDGLSYGIYSGLQVAALTDAGHLTIYAIAAPQNLDKVDAAVREEIALALKDGFS Sbjct: 786 ADRVRQQDGLSYGIYSGLQVAALTDAGHLTIYAIAAPQNLDKVDAAVREEIALALKDGFS 845 Query: 839 EEELQRAKSGILQQRNQQRAGNDNVAATWTLLMNQDRNFLWQQEIDRKLASLTLAQVNAA 898 EEELQRAKSGILQQRNQQRAGNDNVAATWTLLMNQDRNFLWQQEIDRKLASLTLAQVNAA Sbjct: 846 EEELQRAKSGILQQRNQQRAGNDNVAATWTLLMNQDRNFLWQQEIDRKLASLTLAQVNAA 905 Query: 899 LRKYVDPARMTTVIARDEAKVRKP 922 LRKYVDPARMTTVIARDE KVRKP Sbjct: 906 LRKYVDPARMTTVIARDETKVRKP 929 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 134 bits (338), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 114/425 (26%), Positives = 200/425 (47%), Gaps = 23/425 (5%) Query: 50 AEGISEYRFANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPR 109 AE ++ NG K+++ D PT + Y VG E G TG++H LEH+MFKG+ Sbjct: 26 AEPARQFVLGNGMKVIVKEDRRAPTAVQMVWYKVGGIDEVNGLTGVSHALEHMMFKGTRN 85 Query: 110 HP--SIPQDFSKRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDL 167 H + ++ G N T D T Y++ + S +L +A+EADRM N + + Sbjct: 86 HKVGEFSRLVAELGGQENAFTANDFTAYFQQIEKS--HLEKVMALEADRMANLQFDAAEF 143 Query: 168 DSEMTVVRNEFE-AGETSPARVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFY 226 E+ V+ E + P ++ + + + A+ H Y +G D++++ ++++ A+Y Sbjct: 144 AKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMSVQDIAAWY 203 Query: 227 RTYYQPDNAVLLIAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFW-TVEPTQDGERQFVIR 285 R +Y P+NA L++AG DA +VL + FG++ P R LPA EP Q G R+ ++ Sbjct: 204 RQWYAPNNATLVVAGDVDAQRVLALARKYFGKI--PARRLPAGKPQNEPQQLGMRRVTVK 261 Query: 286 RRGDQQLVALAYKMPSALH----PDATALGFAADILTDTPNGRLHKALVETGMATEVYAM 341 + V +A+K P+ D AL + +L N RL +LV TG Sbjct: 262 APAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGYDNARLSASLVRTGGKATAVGA 321 Query: 342 PLEGMAPGLQMMVAKVKVGGDLDAVRQAMISA-----VESFSTQPPTPEEVARLHRE--A 394 G+A G + + G + V A + VE + + + +E+ R+ + A Sbjct: 322 SYSGVARGPVLFTLE---GSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLIA 378 Query: 395 ANSFETLQNNPQQLAVAMSNAIARGDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTV 454 + ++ Q + + M G + I +R++ +++A + A A +YF+ D TV Sbjct: 379 SQVYKRDSVFGQAMEIGMMETAGLGQQNIDRI-IERLKSVTAAQVQAVAQQYFQDDTLTV 437 Query: 455 GLYLP 459 +P Sbjct: 438 ATLVP 442 >gi|300313429|ref|YP_003777521.1| zinc protease-like signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 438 Score = 94.0 bits (232), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 105/422 (24%), Positives = 180/422 (42%), Gaps = 67/422 (15%) Query: 531 VSVSLHLRFGDAQSLFGQRNVAQLTEAMLMRG----TDKLDRRQLADEFARLKIN----- 581 + VS+ G + G+ +A+LT A L RG + L Q+ D FA + Sbjct: 48 LDVSVQFDAGQRRDPAGKAGLAELTVASLTRGVTDASGTLTEAQILDGFADVAAQQHDGA 107 Query: 582 -----GSLYRFQTTRENLVPAIALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTR 636 G R ++ A+ L+ + HP A E+ + +A ++ P+ Sbjct: 108 GQDRAGVSLRTLSSPAEREAALTLLARMLAHPSFPQASLERDRALAIANIKEELTKPEVI 167 Query: 637 AGEALSVHFDHYPAGDWRAAQTLEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFD 696 A +A F H G A ++ A+++A+T E ++ FHR Y A++ +A++GD + Sbjct: 168 AEKA----FMHAAYGSHPYA--MDASEASMQAITREDLQAFHRAHYVANRAVIALIGDIN 221 Query: 697 PAQARSAIDQAFGGWTSAAPYARVLQTWADIPAKRLLVDTPDKENGV-YFARQNIRL--- 752 QAR+ A+ R L A +PA +V E + + A Q+ L Sbjct: 222 LEQARAI----------ASALTRELPQGAALPALPPVVAPKGSEERIAHPASQSHILIGA 271 Query: 753 ---RDTDPDYPALAVANFLLGGSSLKSRLADRVRQQDGLSYGIYSGLQVAALTDAGHLTI 809 + DPD+ AL V N++LGG SRL D VR++ GLSY +YSG + L G I Sbjct: 272 PAIQRGDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGF--SPLAQPGPFQI 329 Query: 810 YAIAAPQNLDKVDAAVREEIALALKDGFSEEELQRAKSGILQQRNQQRAGNDNVAATWTL 869 + + R + +++G + EL+ AK DN+A + L Sbjct: 330 GLQTKKEQTAEALRVTRVTLDKFMQEGPTAAELKAAK--------------DNLAGGFAL 375 Query: 870 LMNQDRNFLWQQEI--------------DRKLASLTLAQVNAALRKYVDPARMTTVIARD 915 ++ + L + ++ ++++ V AA RK+V P ++T+I + Sbjct: 376 RIDSNAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQDVRAAFRKHVHPEELSTIIVGE 435 Query: 916 EA 917 A Sbjct: 436 SA 437 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,923,817 Number of extensions: 129449 Number of successful extensions: 438 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 427 Number of HSP's successfully gapped: 5 Length of query: 922 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 828 Effective length of database: 1,174,780 Effective search space: 972717840 Effective search space used: 972717840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 135 (56.6 bits)