BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0044.p (555 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312190|ref|YP_003776282.1| hemolysin-type calcium binding ... 93 6e-20 gi|300312185|ref|YP_003776277.1| calcium binding secreted hemoly... 64 3e-11 gi|300312186|ref|YP_003776278.1| calcium binding secreted hemoly... 59 2e-09 >gi|300312190|ref|YP_003776282.1| hemolysin-type calcium binding protein [Herbaspirillum seropedicae SmR1] Length = 1139 Score = 92.8 bits (229), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 82/226 (36%), Positives = 108/226 (47%), Gaps = 39/226 (17%) Query: 211 LLGTEGADTL---QVNNFMYKPH---LLRGGAGDDTLRGGAGADMLSGDNGNDSLLGGSG 264 L+GT G DTL N+ ++ L G GDD L GG G D+L G G+D L GG G Sbjct: 436 LVGTTGDDTLWGGAGNDTLFGREGNDRLFGEDGDDQLLGGEGFDLLDGGAGDDQLFGGLG 495 Query: 265 NDIYLFNQGD----GNDTSLQNYFYSAYSRIEKIVFADGTTWANADITTKLTQTGTIGND 320 ND GD G+D S A + I+F K G GND Sbjct: 496 NDQMWGGDGDDTMMGSDVSNTGRPLPAGQTDDDIMFGG---------AGKDGMFGGAGND 546 Query: 321 N-WSGL-------DDQSNRMFGLAGDDQFYGGQLEDTLDGGDGNDSLTG----------- 361 W G ++ ++++G AG+D YGG+ D + GGDG+D + G Sbjct: 547 QMWGGDGDDGLYGNEGDDKLYGEAGNDHIYGGKGNDVIQGGDGDDLIYGDDMEYFWMQPD 606 Query: 362 -YAGNDNLIGGNGNDYLDGGAGSDSLSGGKGNDLLIGGSGNDIYLF 406 G+D L GG GND L GG+G+D L GG GND L+GG G+D Y+ Sbjct: 607 TTGGDDLLYGGAGNDSLFGGSGNDVLDGGSGNDSLVGGLGDDTYVV 652 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 44/102 (43%), Positives = 59/102 (57%), Gaps = 2/102 (1%) Query: 323 SGLDDQSNR--MFGLAGDDQFYGGQLEDTLDGGDGNDSLTGYAGNDNLIGGNGNDYLDGG 380 +G D S + + G GDD +GG DTL G +GND L G G+D L+GG G D LDGG Sbjct: 425 AGSKDNSGKDTLVGTTGDDTLWGGAGNDTLFGREGNDRLFGEDGDDQLLGGEGFDLLDGG 484 Query: 381 AGSDSLSGGKGNDLLIGGSGNDIYLFTTISSAAVAMTTSQVE 422 AG D L GG GND + GG G+D + + +S+ + Q + Sbjct: 485 AGDDQLFGGLGNDQMWGGDGDDTMMGSDVSNTGRPLPAGQTD 526 Score = 63.5 bits (153), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 68/249 (27%), Positives = 93/249 (37%), Gaps = 88/249 (35%) Query: 232 LRGGAGDD---------TLRGGAGADMLSGDNGNDSLLGGSGNDIYLFNQGDGNDTSLQN 282 + GGAG+D L G G D L G+ GND + GG GND+ GDG+D Sbjct: 539 MFGGAGNDQMWGGDGDDGLYGNEGDDKLYGEAGNDHIYGGKGNDV--IQGGDGDD----- 591 Query: 283 YFYSAYSRIEKIVFADGTTWANADITTKLTQTGTIGNDNWSGLDDQSNRMFGLAGDDQFY 342 +++ D D+ Q T G D+ ++G AG+D + Sbjct: 592 -----------LIYGD-------DMEYFWMQPDTTGGDDL---------LYGGAGNDSLF 624 Query: 343 GGQLEDTLDGGDGNDSLTGYAGNDNLI--------------------------------- 369 GG D LDGG GNDSL G G+D + Sbjct: 625 GGSGNDVLDGGSGNDSLVGGLGDDTYVVDGAGDTIREDADQGHDTVVASCSYTLGRNLED 684 Query: 370 -----------GGNGNDYLDGGAGSDS-LSGGKGNDLLIGGSGNDIYLFTTISSAAVAMT 417 GN D L G D+ L GG+G D L+GG+GND Y ++ V Sbjct: 685 LRLAEGGQFNGTGNAQDNLITGNSQDNILDGGRGADTLMGGTGNDSYFVDDVNDKVVEQA 744 Query: 418 TSQVERAMT 426 V+ + Sbjct: 745 GEGVDTVFS 753 Score = 60.8 bits (146), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 55/167 (32%), Positives = 81/167 (48%), Gaps = 39/167 (23%) Query: 231 LLRGGAGDDTLRGGAGADMLSGDNGNDSLLGGSGNDIYLFN-QGD--------GNDTSLQ 281 LL GGAG+D+L GG+G D+L G +GNDSL+GG G+D Y+ + GD G+DT + Sbjct: 613 LLYGGAGNDSLFGGSGNDVLDGGSGNDSLVGGLGDDTYVVDGAGDTIREDADQGHDTVVA 672 Query: 282 NYFYSAYSRIEKIVFADGTTWANADITTKLTQTGTIGNDNWSGLDDQSNRMFGLAGDDQF 341 + Y+ +E + A+G + GT G A D+ Sbjct: 673 SCSYTLGRNLEDLRLAEGGQF-----------NGT-----------------GNAQDNLI 704 Query: 342 YGGQLEDTLDGGDGNDSLTGYAGNDNLIGGNGNDYL--DGGAGSDSL 386 G ++ LDGG G D+L G GND+ + ND + G G D++ Sbjct: 705 TGNSQDNILDGGRGADTLMGGTGNDSYFVDDVNDKVVEQAGEGVDTV 751 >gi|300312185|ref|YP_003776277.1| calcium binding secreted hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 984 Score = 63.9 bits (154), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 36/73 (49%), Positives = 44/73 (60%) Query: 334 GLAGDDQFYGGQLEDTLDGGDGNDSLTGYAGNDNLIGGNGNDYLDGGAGSDSLSGGKGND 393 G GDD YGG +DTL GG + G ND L GG G D L GG G+D L GG G+D Sbjct: 426 GNGGDDVIYGGDGDDTLVGGTYSALARGETDNDVLYGGAGKDSLSGGVGNDLLDGGTGDD 485 Query: 394 LLIGGSGNDIYLF 406 + GG+G+DIY+ Sbjct: 486 RMYGGAGDDIYVV 498 Score = 57.4 bits (137), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 43/127 (33%), Positives = 62/127 (48%), Gaps = 19/127 (14%) Query: 231 LLRGGAGDDTLRGGAGADMLSGDNGNDSLLGGSGNDIY--------LFNQGD-GNDTSLQ 281 +L GGAG D+L GG G D+L G G+D + GG+G+DIY +F D G DT L Sbjct: 459 VLYGGAGKDSLSGGVGNDLLDGGTGDDRMYGGAGDDIYVVDSAGDGVFENADAGRDTVLA 518 Query: 282 NYFYSAYSRIEKIVFADGTTWANADITTKLTQTGTIGNDNWSGLDDQSNRMFGLAGDDQF 341 + +E + +G ++ TG ++ +G + Q N + GLAG D Sbjct: 519 GMSTTLGENVEDLRLLEGGSY---------NGTGNFLDNRLTG-NSQDNILDGLAGADTM 568 Query: 342 YGGQLED 348 GGQ D Sbjct: 569 MGGQGND 575 >gi|300312186|ref|YP_003776278.1| calcium binding secreted hemolysin protein [Herbaspirillum seropedicae SmR1] Length = 1080 Score = 58.5 bits (140), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 35/74 (47%), Positives = 43/74 (58%), Gaps = 9/74 (12%) Query: 334 GLAGDDQFYGGQLEDTLDGGDGNDSLTGYAGNDNLIGGNGNDYLDGGAGSDSLSGGKGND 393 G AGDDQ G ED+L GGDG D L G+ +GND LDGG G+D+L GG G+D Sbjct: 539 GTAGDDQLLGSTGEDSLHGGDGKDWLFGF---------DGNDVLDGGLGADTLYGGDGDD 589 Query: 394 LLIGGSGNDIYLFT 407 I S +D + T Sbjct: 590 TYIVNSSDDRVVET 603 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,512,359 Number of extensions: 56424 Number of successful extensions: 318 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 249 Number of HSP's successfully gapped: 16 Length of query: 555 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 465 Effective length of database: 1,193,720 Effective search space: 555079800 Effective search space used: 555079800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)