BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0032.p (478 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 946 0.0 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 211 1e-55 gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipo... 201 7e-53 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 199 5e-52 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 198 7e-52 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 946 bits (2446), Expect = 0.0, Method: Compositional matrix adjust. Identities = 477/478 (99%), Positives = 477/478 (99%) Query: 1 MLPAFTRVALAAVLVLAVGGCAMREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDW 60 MLPAFTRVALAA LVLAVGGCAMREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDW Sbjct: 15 MLPAFTRVALAAALVLAVGGCAMREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDW 74 Query: 61 WKQFGSNELSELVEQGQRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPI 120 WKQFGSNELSELVEQGQRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPI Sbjct: 75 WKQFGSNELSELVEQGQRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPI 134 Query: 121 SGGTPTNTANASLQVSYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQ 180 SGGTPTNTANASLQVSYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQ Sbjct: 135 SGGTPTNTANASLQVSYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQ 194 Query: 181 VLSLRDRLAIARQNVQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQE 240 VLSLRDRLAIARQNVQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQE Sbjct: 195 VLSLRDRLAIARQNVQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQE 254 Query: 241 QDAQTALAILLGRPPQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANA 300 QDAQTALAILLGRPPQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANA Sbjct: 255 QDAQTALAILLGRPPQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANA 314 Query: 301 NVAVARAALFPSITLTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLA 360 NVAVARAALFPSITLTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLA Sbjct: 315 NVAVARAALFPSITLTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLA 374 Query: 361 IAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAG 420 IAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAG Sbjct: 375 IAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAG 434 Query: 421 AEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDSQAPGDSGTSSVS 478 AEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDSQAPGDSGTSSVS Sbjct: 435 AEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDSQAPGDSGTSSVS 492 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 211 bits (537), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 161/465 (34%), Positives = 231/465 (49%), Gaps = 16/465 (3%) Query: 20 GCAMREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDWWKQFGSNELSELVEQGQRN 79 GCA T P ++M P W A P WW++FG +L+ L +Q Sbjct: 18 GCASGPDYTRPQVDM--PAAWTSEAPWRSMQPADATPRGPWWERFGDAQLNALQQQALAG 75 Query: 80 NYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPISGGTP---TNTANASL--- 133 N +A A +R+ QA AQ + GA P +NA AAR IS P N+ N S Sbjct: 76 NQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSR-ISANRPLTNYNSPNFSTVQN 134 Query: 134 ------QVSYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQVLSLRDR 187 VSYE+DF+G+ + VEAA A + D E L +T+ + + Y + L Sbjct: 135 DFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNLRELDVE 194 Query: 188 LAIARQNVQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQEQDAQTAL 247 L + R+ + + L L A+ G+ S LD+A+Q++ L + I L +Q + A+ Sbjct: 195 LDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRAQYEHAI 254 Query: 248 AILLGRPPQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANANVAVARA 307 A L G P F + L I P I G+PS++L RRPDI AE ++AAANA + VA A Sbjct: 255 ATLTGTPAPAFSITPS-LTPITPPAIPVGVPSDVLERRPDIAAAERAMAAANAQIGVASA 313 Query: 308 ALFPSITLTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLAIAQKEEL 367 A +PS L S G S +LF+ P+L +LG +FDGG+LR D + A E Sbjct: 314 AYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRAGVDFSQAGYEAT 373 Query: 368 VQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVV 427 V YR+ V+T++ EVE + + +LE Y + AR E+A RY GA L V Sbjct: 374 VASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASSRYEGGATPYLDV 433 Query: 428 LDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDSQAPGDS 472 + Q++L ++ Q Q+ QRL +V L KALGG WQ P ++ Sbjct: 434 ITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRTPPAEA 478 >gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 470 Score = 201 bits (512), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 143/447 (31%), Positives = 229/447 (51%), Gaps = 15/447 (3%) Query: 19 GGCAMREVQTDPILEMQVPDKW---GGGASADVAHAEQLWPDTDWWKQFGSNELSELVEQ 75 G C+ + Q P W G ADV WW+ FG L+ L+++ Sbjct: 24 GACSPLTRSAYTPVSTQTPASWQQRGSQPEADVQR---------WWQGFGDATLTALIDE 74 Query: 76 GQRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPISGG-TPTNTANASLQ 134 R N ++A A ++++A+AQA +A +P+ + GA+ + G T + +S++ Sbjct: 75 ALRKNNDLALAAIKLRRAQAQAGLADDAFVPALSASLGASSSKQLDGDRISTRASQSSVK 134 Query: 135 VSYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGIVSTYLQVLSLRDRLAIARQN 194 +SYE+D WG+ A + + A DR++ ALT+ + Y L R+A A Q+ Sbjct: 135 LSYEVDLWGRLAAASDLSRWEALATEQDRQSTALTLIGTTANLYWTGAYLNQRVATAEQS 194 Query: 195 VQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIPDLQQQEQDAQTALAILLGRP 254 + AE+ L LV Q +AG+ S +++ + L +Q+ +L QQ + ALAIL +P Sbjct: 195 IAYAEKTLELVRTQYQAGAVSSVEVLSAQQNLYSQQSSYTELVQQRVENDNALAILFDQP 254 Query: 255 PQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIRRAEASLAAANANVAVARAALFPSIT 314 P L +P ++AG+P+ELL RRPD+R E + +A A V AR +P ++ Sbjct: 255 PGKAIALPARLPEQALPVLAAGVPAELLGRRPDLRALELRIRSALAGVDQARLNYYPQLS 314 Query: 315 LTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFDGGKLRGQRDLAIAQKEELVQVYRQR 374 LTG+ G S ALL L P A LG L P +++ + ++ A + V +R+ Sbjct: 315 LTGTLGTSSDALLRLVQNP--VATLGGALTLPFLQHSEMKFKVGISQADFDTAVLNFRKG 372 Query: 375 VITSLSEVEKALGAIRSLEERYRLKVGEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTL 434 + T+ SEVE AL A + + L V +D A+ A L E+RYRAG+ L + LD Q Sbjct: 373 LYTAFSEVENALSARQQYARQQALLVRNLDAAQAAERLLEVRYRAGSATLQLWLDQQDKR 432 Query: 435 SEAQNQLGQIKLQRLQATVSLYKALGG 461 A+ L Q++L +L+ ++LY+ALGG Sbjct: 433 RTAEISLAQVRLNQLKNQMTLYQALGG 459 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 199 bits (505), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 151/418 (36%), Positives = 224/418 (53%), Gaps = 14/418 (3%) Query: 59 DWWKQFGSNELSELVEQGQRNNYEIAAAYSRVKQAEAQARIAGAPLLP--SAGINAGAAR 116 +WWK FG +L+ L + Q+ N ++ AA +R+ QA A R A A P AG+ A R Sbjct: 77 EWWKIFGDEKLNALEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQR 136 Query: 117 EIPISGGTPTNTAN-------ASLQVSYEIDFWGKNRAGVEAAEATLRANRYDRETVALT 169 P S G P + A + VSYE+D +G+ + V+AA A + + +V L Sbjct: 137 PSPASQGLPADADTRPNTLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLA 196 Query: 170 VTSGIVSTYLQVLSLRDRLAIARQNVQNAERVLRLVEAQSRAGSASPLDLARQRSALANQ 229 + + + Y V L A+ R V + LRLV+ + G S +D+AR R+ LA+ Sbjct: 197 LQADVAQQYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASA 256 Query: 230 RQLIPDLQQQEQDAQTALAILLGRPPQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIR 289 + + +Q A+ ALAILLGR P F + + L R+ + + GLPS LL RRPDI Sbjct: 257 QSEALGIARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVT-VPPGLPSALLERRPDIA 315 Query: 290 RAEASLAAANANVAVARAALFPSITLTGSTGAQSSALLSLFDGPN----LFANLGAGLIA 345 AE ++AAANA V VA++A FPS+ LTG G +S+ L LF+ + L +G L Sbjct: 316 AAERAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSM 375 Query: 346 PIFDGGKLRGQRDLAIAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQ 405 P+FDGG+ + D A A EE V YR V+ + EVE L +R L+++ R + V Sbjct: 376 PLFDGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAA 435 Query: 406 ARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGW 463 + A +L+ +YR GA V+D RT+ + Q + +R ++TV+L +ALGGGW Sbjct: 436 SARAAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 198 bits (504), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 154/477 (32%), Positives = 246/477 (51%), Gaps = 24/477 (5%) Query: 3 PAFTRVALAAVLVLAVGGCAMREVQTDPILEMQVPDKWGGGASADVAHAEQLWPDTDWWK 62 P + A V+ +GGC++ P Q P + AD A +L W+ Sbjct: 10 PRLLHMCAALVMAGVLGGCSLAPTYERP----QAPVE--SSYPADAAGRAELQAVNLGWR 63 Query: 63 QFGSNE-LSELVEQGQRNNYEIAAAYSRVKQAEAQARIAGAPLLPSAGINAGAAREIPIS 121 QF ++ L +L+ NN ++ A +++A AQ +IA A LLP+ ++ AAR + Sbjct: 64 QFFPDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAA 123 Query: 122 G----GTPT--NTANASLQV-SYEIDFWGKNRAGVEAAEATLRANRYDRETVALTVTSGI 174 G PT N+ L V +YE+DF+G+ R +AA A+ + +++ +++ + + Sbjct: 124 SQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQV 183 Query: 175 VSTYLQVLSLRDRLAIARQNVQNAERVLRLVEAQSRAGSASPLDLARQRSALANQRQLIP 234 YL S ++L +A + +++ + L L + + G+++ LD ++ + + R Sbjct: 184 AQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKT 243 Query: 235 DLQQQEQDAQTALAILLGR-----PPQNFKVEEKGLARIQMPRISAGLPSELLTRRPDIR 289 L++Q A+ AL +L+G+ PP +EK + I AGLPS+LLT RPDIR Sbjct: 244 QLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDI-----PAGLPSDLLTNRPDIR 298 Query: 290 RAEASLAAANANVAVARAALFPSITLTGSTGAQSSALLSLFDGPNLFANLGAGLIAPIFD 349 AE SL AANAN+ ARAA FP I+LTG+ G+ SS L LF+ + + G L PIFD Sbjct: 299 SAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFD 358 Query: 350 GGKLRGQRDLAIAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGEVDQARFA 409 G+ DLA +K + V Y + + T+ EV AL A SL+E+ + + + + Sbjct: 359 FGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQER 418 Query: 410 FELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDS 466 +L ++R++ G L LD QR L AQ L Q + RL + LYKALGGG ++ Sbjct: 419 LKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNEN 475 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,342,756 Number of extensions: 54461 Number of successful extensions: 326 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 19 Length of query: 478 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 389 Effective length of database: 1,198,455 Effective search space: 466198995 Effective search space used: 466198995 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)