BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0031.p (356 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Her... 702 0.0 gi|300312796|ref|YP_003776888.1| 2-nitropropane dioxygenase [Her... 205 4e-54 gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Her... 67 2e-12 >gi|300312741|ref|YP_003776833.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 702 bits (1812), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/356 (98%), Positives = 352/356 (98%) Query: 1 MHTPTPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQL 60 MHTPTPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQL Sbjct: 1 MHTPTPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQL 60 Query: 61 QALTEKPYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQM 120 ALTEKPYN+NVFCHQPAVLDSERDRAWL+HLQPLLDEFGGRIAAPLKEIYQSFN DTQM Sbjct: 61 HALTEKPYNINVFCHQPAVLDSERDRAWLAHLQPLLDEFGGRIAAPLKEIYQSFNADTQM 120 Query: 121 QQVVLEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGF 180 QQVVLEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGF Sbjct: 121 QQVVLEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGF 180 Query: 181 EAGGHRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMG 240 EAGGHRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDG GIAAALQLGAQAVQMG Sbjct: 181 EAGGHRGIFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGHGIAAALQLGAQAVQMG 240 Query: 241 TAFLLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAY 300 TAFLLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAY Sbjct: 241 TAFLLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAY 300 Query: 301 SAAYDAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA 356 AAYDAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA Sbjct: 301 PAAYDAGKQLHALAAARDVHEFGAHWAGQGAALIRDGYTVAELVHLLVQEWQAQQA 356 >gi|300312796|ref|YP_003776888.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 358 Score = 205 bits (522), Expect = 4e-54, Method: Compositional matrix adjust. Identities = 150/353 (42%), Positives = 199/353 (56%), Gaps = 8/353 (2%) Query: 5 TPLMQRLGLAHPIIQAPMAGSSTVVMAAAASNAGALGSLALGASNAEQAREALAQLQALT 64 TPL ++ G+ PI+Q PM G ST M AA SNAG +G+LA + + R + +++LT Sbjct: 7 TPLTRKFGIDFPIVQGPMNGGSTPEMVAAVSNAGGIGALAAANFDVQTMRTKVNAIRSLT 66 Query: 65 EKPYNVNVFCHQPAVLDSERDRAWLSHLQPLLDEFGGRIAAPLKEIYQSFNTDTQMQQVV 124 KP+ VN+F + R L LQP+ E G AA + + F Q+ ++ Sbjct: 67 TKPFCVNLFVLETPAPTPGEIRDALDRLQPIRAELGLPPAAAPERYCEEFRE--QLDALI 124 Query: 125 LEHAPAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGG 184 AP V SF FGL A I L AG L++ TATN+ EA +A G DAI AQG EAGG Sbjct: 125 ALKAP-VASFTFGLLDAASIERLHAAGSLVIGTATNVAEALAWQANGADAICAQGAEAGG 183 Query: 185 HRGIFDPTKD-EQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAF 243 HRG F D IG+ AL+ +V LPVIAAGGIMDG+GIAAAL LGAQA Q+GTAF Sbjct: 184 HRGTFIGHPDASMIGTLALVPQIVDAVALPVIAAGGIMDGRGIAAALTLGAQAAQLGTAF 243 Query: 244 LLTRESNANASYRALLKSPRAQHTRVTAAISGRPARGIVNRFMEEIDTPAAPPIPAYSAA 303 L+T ES +A+++ ++ TR T +GRPARGI+N FM + +PAY Sbjct: 244 LMTAESPIHAAWQEKIRLSTDTSTRTTRTFTGRPARGILNDFMLRMGR-YEQELPAYPVQ 302 Query: 304 YDAGKQLHALAAARDVHEFGAHWAGQGAALIR---DGYTVAELVHLLVQEWQA 353 + ++ A + EF + WAGQ A + R + AELV +LV E QA Sbjct: 303 HALTTEIRKAAGQANRPEFMSLWAGQAAGMSRRRAEAIPAAELVRVLVAETQA 355 >gi|300313977|ref|YP_003778069.1| 2-nitropropane dioxygenase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 95/354 (26%), Positives = 154/354 (43%), Gaps = 63/354 (17%) Query: 16 PIIQAPMAGSSTVVMAAAASNAGALGSL-ALGASNAEQAREALAQLQALTEKPYNVNVFC 74 PII +PM +S + AA AG +GS AL A AE Sbjct: 17 PIICSPMFIASGPALVAAQCKAGLVGSFPALNARPAE----------------------- 53 Query: 75 HQPAVLDSERDRAWLSHLQPLLD----EFGGRIAAPL---KEIYQSFNTDTQMQQVVLEH 127 +LD+ WL+ LQ L E R P+ + ++QS + Q ++H Sbjct: 54 ----LLDT-----WLTQLQEELAQWQAEHAQRKVGPIAVNQIVHQSNDRLAQDVATCVKH 104 Query: 128 APAVVSFHFGLPPADVIGALRQAGILLMATATNLDEARQIEAAGLDAIVAQGFEAGGHRG 187 ++ PP +++ A+ G +++ +L A + AG+D ++ AGGH G Sbjct: 105 QVPIIISSLRAPPQEMLDAVHSYGGIVLHDVISLRHAEKALEAGVDGLILVAAGAGGHAG 164 Query: 188 IFDPTKDEQIGSFALLRLLVQHTRLPVIAAGGIMDGQGIAAALQLGAQAVQMGTAFLLTR 247 P FAL+ + + + P++ +G I G + AA +GA MG+ +L T+ Sbjct: 165 TLSP--------FALVGEVRKMFKGPLVLSGAIATGDAVLAAQAMGADFAYMGSRWLATQ 216 Query: 248 ESNANASYR-ALLKSPRAQ--HTRVTAAISGRP-ARGIVNRFMEEIDTPAAPPIPAYSAA 303 ESN + +YR A++ S A +T + + G + IVN ++ + P A + Sbjct: 217 ESNVDQAYRQAIVDSTAADVVYTNLFTGVHGNYLKKSIVNAGLDPDNLPEADKTKMNFGS 276 Query: 304 YDAGKQLHALAAARDVHEFGAHW-AGQGAALIRDGYTVAELVHLLVQEWQAQQA 356 K A RD+ W AGQG L+ D TVAE+V L E++A ++ Sbjct: 277 GGGSKA----KAWRDI------WGAGQGVGLMDDVPTVAEMVDRLEAEYRAARS 320 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 987,579 Number of extensions: 35687 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 3 Length of query: 356 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 270 Effective length of database: 1,212,660 Effective search space: 327418200 Effective search space used: 327418200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)