BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0006.0027.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 525 e-150 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 129 3e-31 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 120 1e-28 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 111 6e-26 gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 110 1e-25 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 525 bits (1353), Expect = e-150, Method: Compositional matrix adjust. Identities = 259/261 (99%), Positives = 260/261 (99%) Query: 1 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGHAPVSYSR 60 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGHAPVSYSR Sbjct: 1 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGHAPVSYSR 60 Query: 61 TDVSSEREVDAMFADLAQRWSGRLDVLVNNAGIAGPTLPVEEVALSDWDQTLAVNLTGPF 120 TDVSSEREVDAMFADLAQRWSGRLDVL+NNAGIAGPT PVEEVALSDWDQTLAVNLTGPF Sbjct: 61 TDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPF 120 Query: 121 LCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIR 180 LCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIR Sbjct: 121 LCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIR 180 Query: 181 VNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCS 240 VNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCS Sbjct: 181 VNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCS 240 Query: 241 PAGASISGQSLSVCGNVEVLG 261 PAGASISGQSLSVCGNVEVLG Sbjct: 241 PAGASISGQSLSVCGNVEVLG 261 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 129 bits (324), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 83/258 (32%), Positives = 144/258 (55%), Gaps = 14/258 (5%) Query: 5 LKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARA------RFGHAPVSY 58 LKD+ ++T A GIG I + GA V I D++ L +A+A + G ++ Sbjct: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLA---LEAAKATAEEIVKEGGTAMAV 59 Query: 59 SRTDVSSEREVDAMFADLAQRWSGRLDVLVNNAGIAGPTLPVEEVALSDWDQTLAVNLTG 118 + +V+ E +VD AD + G LD++++NAGI + PV E++L +W + LA++L G Sbjct: 60 A-MNVTDETQVDKGVADAVNTYGG-LDIMISNAGIQIIS-PVAELSLENWRKMLAIHLDG 116 Query: 119 PFLCTRRAVPLLKKNG-GGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPS 177 FL TR A+ + K G GG+I+ + SV L L+ PY +K+G++GL +T A E Sbjct: 117 AFLTTRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKD 176 Query: 178 HIRVNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLR-KMVTAEDIANQVI 236 IR N + PG V E+ + +A +GI E++ ++++ + ++ + T +D+A + Sbjct: 177 KIRTNVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAV 236 Query: 237 FLCSPAGASISGQSLSVC 254 FL + +++GQS+ V Sbjct: 237 FLAAFPTNALTGQSVVVS 254 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 120 bits (301), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 94/261 (36%), Positives = 133/261 (50%), Gaps = 21/261 (8%) Query: 1 MDLNLKDQKV-IVTAGAQGIGLAITAAFVEAGAHVHICDVSEDF---LASARARFGH--A 54 M+ N +V +VT GA GIG +I+ G V + D++ D A A GH A Sbjct: 1 MNRNDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAA 60 Query: 55 PVSYSRTDVSSEREVDAMFADLAQRWSGRLDVLVNNAGIAGPTLPVEEVALSDWDQTLAV 114 P+ + D+ S + A FA + + + GR DVLVNNAG+A T + L +W QT+ V Sbjct: 61 PL---QMDLGSPESIAAAFAHIEKDY-GRCDVLVNNAGVA-RTYSFLDYPLENWLQTMNV 115 Query: 115 NLTGPFLCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIEL 174 N+TG L + A L+ K G IVNISS++G A RT Y SK VIGLT AIEL Sbjct: 116 NVTGVLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIEL 175 Query: 175 GPSHIRVNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQ 234 I VN+V PG V+ + + E+ R+ V +R+ T ++IA+ Sbjct: 176 AQYGITVNSVAPGPVDTPMTQ----------AMHSEQTRESYYRLVPMRRYGTTDEIASA 225 Query: 235 VIFLCSPAGASISGQSLSVCG 255 V +L + A I+G + V G Sbjct: 226 VCYLAAEESAYITGHVIPVDG 246 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 111 bits (278), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 81/257 (31%), Positives = 128/257 (49%), Gaps = 13/257 (5%) Query: 3 LNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSE----DFLASARARFGHAPVSY 58 + L+D+ IVT G G G I A+ GA + + D+ E + +A G A + Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRA--VF 58 Query: 59 SRTDVSSEREVDAMFADLAQRWSGRLDVLVNNAGIAGPTLPVEEVALSDWDQTLAVNLTG 118 ++ DVS ++D + A + + G+LD++VNNAG P+ EV ++D+ AVN+ Sbjct: 59 AKADVSKRADMDQLLATALEHF-GKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKS 117 Query: 119 PFLCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSH 178 FL + VP ++ GGGA +NI+S AG T Y+ SK VI +++ A ELGP Sbjct: 118 IFLSAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDK 177 Query: 179 IRVNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFL 238 IRVN V P I + E R++ ++ + + + T EDIAN ++L Sbjct: 178 IRVNCVNPVISATGLLSEFMGVPDTP------ENRKKFVATIPMGRFSTPEDIANACLYL 231 Query: 239 CSPAGASISGQSLSVCG 255 S ++G + V G Sbjct: 232 GSDEAEFVTGVCIEVDG 248 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 110 bits (275), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 76/253 (30%), Positives = 128/253 (50%), Gaps = 13/253 (5%) Query: 4 NLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGH-APVSYSRTD 62 + + + I+T GAQG G A+ ++ A V + D+ E LA AR + V + D Sbjct: 5 DFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVETVKVD 64 Query: 63 VSSEREVDAMFADLAQRWSGRLDVLVNNAGIAGPTLPVEEVALSDWDQTLAVNLTGPFLC 122 +SS+ EV + ++ + +D+LV++AGIAG PV E + +W + + ++L G F Sbjct: 65 ISSQAEVQQAI-EATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAFYV 123 Query: 123 TRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIRVN 182 + V + G IVNI+S+AG+ G + YSA+K G+I LT++ E +I VN Sbjct: 124 NQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNIAVN 183 Query: 183 AVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCSPA 242 A+ P A Q RI+ A+ I + +LS++ + V E++A V +L S Sbjct: 184 AITP----AAAQTRILEQCTQAH-IDY------MLSKIPRARFVKVEELAAMVAWLVSEE 232 Query: 243 GASISGQSLSVCG 255 + + + G Sbjct: 233 NSFTTASVFDLSG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 803,684 Number of extensions: 33536 Number of successful extensions: 275 Number of sequences better than 1.0e-08: 47 Number of HSP's gapped: 183 Number of HSP's successfully gapped: 47 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)