BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0006.0024.p
         (232 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312735|ref|YP_003776827.1| DedA transmembrane protein [Her...   425   e-120
gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protei...   214   4e-57
gi|300310491|ref|YP_003774583.1| transmembrane protein [Herbaspi...    54   1e-08

>gi|300312735|ref|YP_003776827.1| DedA transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 213

 Score =  425 bits (1092), Expect = e-120,   Method: Compositional matrix adjust.
 Identities = 213/213 (100%), Positives = 213/213 (100%)

Query: 20  MDILLFLVDFIVHIDRHLAELAASYGPWLFLILFLIIFCETGLVVTPILPGDSLLFVTGA 79
           MDILLFLVDFIVHIDRHLAELAASYGPWLFLILFLIIFCETGLVVTPILPGDSLLFVTGA
Sbjct: 1   MDILLFLVDFIVHIDRHLAELAASYGPWLFLILFLIIFCETGLVVTPILPGDSLLFVTGA 60

Query: 80  LAATGAYDINLMVLTLIVAAILGDSTNYQIGKMLGIKVFDKPNSRIFKKEYLDKTHAFFE 139
           LAATGAYDINLMVLTLIVAAILGDSTNYQIGKMLGIKVFDKPNSRIFKKEYLDKTHAFFE
Sbjct: 61  LAATGAYDINLMVLTLIVAAILGDSTNYQIGKMLGIKVFDKPNSRIFKKEYLDKTHAFFE 120

Query: 140 KHGGKAIIIARFAPIVRTFAPFVAGVGAMTYTRFFLYNVVGGIAWVASFAYAGYFFGNMP 199
           KHGGKAIIIARFAPIVRTFAPFVAGVGAMTYTRFFLYNVVGGIAWVASFAYAGYFFGNMP
Sbjct: 121 KHGGKAIIIARFAPIVRTFAPFVAGVGAMTYTRFFLYNVVGGIAWVASFAYAGYFFGNMP 180

Query: 200 IVKKNLSLLIVAIVILSILPGIIEYIRHKRRPG 232
           IVKKNLSLLIVAIVILSILPGIIEYIRHKRRPG
Sbjct: 181 IVKKNLSLLIVAIVILSILPGIIEYIRHKRRPG 213


>gi|300310171|ref|YP_003774263.1| DedA DSG-1 transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 220

 Score =  214 bits (546), Expect = 4e-57,   Method: Compositional matrix adjust.
 Identities = 109/218 (50%), Positives = 148/218 (67%), Gaps = 9/218 (4%)

Query: 20  MDILLFLVDFIVHIDRHLAELAASYGPWLFLILFLIIFCETGLVVTPILPGDSLLFVTGA 79
           MD L  L+D +VH+D++L  L   YG  ++ +LFLI+FCETGLVV P LPGDSLLF+ G 
Sbjct: 1   MDYLQ-LLDVLVHVDKYLGVLIEQYGALIYAVLFLIVFCETGLVVLPFLPGDSLLFIAGT 59

Query: 80  LAATGAYDINLMVLTLIVAAILGDSTNYQIGKMLGIKVFDKPNSRIFKKEYLDKTHAFFE 139
             ATGA DI L++  L+VAA+ G++ NY IG  +G +V+   N R    E L KTHAF+E
Sbjct: 60  FCATGAMDIWLLMTLLVVAAVAGNTLNYWIGGWVGQRVYTH-NYRWLNPEALRKTHAFYE 118

Query: 140 KHGGKAIIIARFAPIVRTFAPFVAGVGAMTYTRFFLYNVVGGIAWVASFAYAGYFFGNMP 199
           KHGGK I+IARF PIVRTFAPF+AGV AM++ RF L+NV+G   WV      GY FGN+P
Sbjct: 119 KHGGKTIVIARFTPIVRTFAPFIAGVSAMSFARFQLFNVLGAFLWVVGLVVLGYLFGNIP 178

Query: 200 IVKKNLSLLIVAIVILSILP-------GIIEYIRHKRR 230
           +V++NL+ L++  ++ ++ P        I   +R KR+
Sbjct: 179 VVQQNLNTLVLIGIVAAVFPIVLGGLWQIWRKLRGKRK 216


>gi|300310491|ref|YP_003774583.1| transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 307

 Score = 54.3 bits (129), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 40/163 (24%), Positives = 73/163 (44%), Gaps = 10/163 (6%)

Query: 36  HLAELAASYGPWLFLILFLIIFCETGLVVTPILPGDSLLFVTGALAATGAYDINLMVLTL 95
           HL E    YG    LI+F+ +  E      P  P    L +TGAL+A G Y   +++L  
Sbjct: 6   HLIE---QYG---LLIVFVNVLVEQLGAPVPAYP---TLILTGALSAAGRYSPAVLLLVA 56

Query: 96  IVAAILGDSTNYQIGKMLGIKVFDK-PNSRIFKKEYLDKTHAFFEKHGGKAIIIARFAPI 154
           + AA+L D   Y  G+  G ++  +     +     + +T + + + G +++++A+F P 
Sbjct: 57  VCAALLADYVWYLAGRRYGRQIMSRLCRISLSPDSCVRQTESIYLRWGARSLLLAKFIPG 116

Query: 155 VRTFAPFVAGVGAMTYTRFFLYNVVGGIAWVASFAYAGYFFGN 197
             + A  ++G        F L++  G   W     + G  F +
Sbjct: 117 FASIASALSGTMGTRRRSFLLWDAAGSALWAGLGIFLGSLFSS 159


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.332    0.148    0.449 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 657,432
Number of extensions: 25200
Number of successful extensions: 85
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 84
Number of HSP's successfully gapped: 3
Length of query: 232
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 150
Effective length of database: 1,231,600
Effective search space: 184740000
Effective search space used: 184740000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 129 (54.3 bits)