BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0004.0001.p (498 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310312|ref|YP_003774404.1| hypothetical protein Hsero_0979... 105 1e-23 >gi|300310312|ref|YP_003774404.1| hypothetical protein Hsero_0979 [Herbaspirillum seropedicae SmR1] Length = 613 Score = 105 bits (261), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 57/145 (39%), Positives = 79/145 (54%) Query: 293 MKSADLPRSNDPDRLAQALKATDYAWGNGPSPFASLTRNEVVAIYYDDSGTYTRDERMAA 352 + A+LP S +P RLA+A +AT+ +G +PFA L R E+ AI YDDSG YT +ER AA Sbjct: 120 LADAELPPSQEPQRLARARQATESLYGRAANPFAGLGRAELSAIVYDDSGAYTINERYAA 179 Query: 353 VKQLNEIDRNLWDGLTTRAANTGDWREVNEMGLKNYDDLLPIEKMRYPANYKMQLQQLLA 412 Q E+D+ W + RA +G+W+ V L Y L +E+ YP NY LQQ L Sbjct: 180 ANQRTELDQRFWAPVFQRALESGNWQPVITAALVFYVSLSALERTAYPDNYPQLLQQYLD 239 Query: 413 YDNAKAGGALSKEQKKTLLEMMDAL 437 + L Q+ L M++ L Sbjct: 240 LYRLQPQAVLPAPQQADLARMLETL 264 Score = 85.1 bits (209), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 47/120 (39%), Positives = 71/120 (59%), Gaps = 6/120 (5%) Query: 94 DAEVPNTDDPTLLARAKQATDSINGKGANPFAGLSRDQLALIAYDEGGSFTVNERKAALD 153 DAE+P + +P LARA+QAT+S+ G+ ANPFAGL R +L+ I YD+ G++T+NER AA + Sbjct: 122 DAELPPSQEPQRLARARQATESLYGRAANPFAGLGRAELSAIVYDDSGAYTINERYAAAN 181 Query: 154 EAQNQESQWRQQTFAKAMSAYKGGEGANSREAGKQLLDHLKSLSPIEQAQYPQSYAVQLQ 213 + + ++ F +A+ E N + L SLS +E+ YP +Y LQ Sbjct: 182 QRTELDQRFWAPVFQRAL------ESGNWQPVITAALVFYVSLSALERTAYPDNYPQLLQ 235 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.308 0.123 0.340 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,428,057 Number of extensions: 56377 Number of successful extensions: 180 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 179 Number of HSP's successfully gapped: 2 Length of query: 498 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 409 Effective length of database: 1,198,455 Effective search space: 490168095 Effective search space used: 490168095 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 132 (55.5 bits)