BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0134.p (522 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 1009 0.0 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 218 1e-57 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 215 9e-57 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 117 2e-27 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 111 1e-25 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 1009 bits (2608), Expect = 0.0, Method: Compositional matrix adjust. Identities = 514/514 (100%), Positives = 514/514 (100%) Query: 9 MAAAGANGEKADAAAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATA 68 MAAAGANGEKADAAAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATA Sbjct: 1 MAAAGANGEKADAAAWLAVAAGTLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATA 60 Query: 69 YLVAEIVIIPMSAWLERLLGLRVLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGG 128 YLVAEIVIIPMSAWLERLLGLRVLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGG Sbjct: 61 YLVAEIVIIPMSAWLERLLGLRVLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGG 120 Query: 129 ALIPTAMTIIATRLPRSQQPVGNAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPV 188 ALIPTAMTIIATRLPRSQQPVGNAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPV Sbjct: 121 ALIPTAMTIIATRLPRSQQPVGNAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPV 180 Query: 189 GAALITLLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRW 248 GAALITLLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRW Sbjct: 181 GAALITLLLVAIPYERPKFEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRW 240 Query: 249 LAATSVLGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLS 308 LAATSVLGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLS Sbjct: 241 LAATSVLGFILLAIGQVRARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLS 300 Query: 309 GIAGYNSLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSP 368 GIAGYNSLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSP Sbjct: 301 GIAGYNSLQSGWIVLLSGVPMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSP 360 Query: 369 LSYGGSFVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAI 428 LSYGGSFVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAI Sbjct: 361 LSYGGSFVASQLMRGVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAI 420 Query: 429 AVIQDQRLWLHSRRLEESLNANSDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTY 488 AVIQDQRLWLHSRRLEESLNANSDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTY Sbjct: 421 AVIQDQRLWLHSRRLEESLNANSDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTY 480 Query: 489 ADLFWMLGVAILCVTPLVLFLRPLPTHAAPVASH 522 ADLFWMLGVAILCVTPLVLFLRPLPTHAAPVASH Sbjct: 481 ADLFWMLGVAILCVTPLVLFLRPLPTHAAPVASH 514 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 218 bits (555), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 141/471 (29%), Positives = 240/471 (50%), Gaps = 8/471 (1%) Query: 31 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLR 90 +L M LD SI N ++PTI G +G S EGTW+ T++ A + IP++ WL + LG Sbjct: 30 SLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAV 89 Query: 91 VLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVG 150 L +T+ LF L S LCGL+ TLP+++ RV QG G ++P + +++ P+S+ Sbjct: 90 RLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFA 149 Query: 151 NAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVG--AALITLLLVAIPYERPKFE 208 A++G TA++ PV GP +GGW+T++ +W + F+IN+PVG AA +TL AI R Sbjct: 150 LALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL---AIYRSRETPT 206 Query: 209 QLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRAR 268 D G+A L + ++L++G+ WF+SP I L +++ F + ++ Sbjct: 207 HKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNND 266 Query: 269 HPVIQLRLLRDRQF-GSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGV 327 HPV+ LRL R F G + +++ GV +G ++PQ+L GY S+ SG G+ Sbjct: 267 HPVVDLRLFGRRNFAGGTIAISIGYGV-FFGNLVILPQWLQQYLGYRSIDSGLSTAPIGI 325 Query: 328 PMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGSFVASQLMRGVGTV 387 +++MP + LVD R F+ A ++ + + + + L++G+ T Sbjct: 326 FAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTA 385 Query: 388 LAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESL 447 + + L + +PP + A+GL S R G++ + + R +H +L E Sbjct: 386 MFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRSIMHHAQLTEHA 445 Query: 448 NANSDLVQSSMTAQGHLLG-GQDVALQLMGATIQSQALTMTYADLFWMLGV 497 S +Q ++ LG + ++ TI QA + D+F++ V Sbjct: 446 TPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAV 496 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 215 bits (547), Expect = 9e-57, Method: Compositional matrix adjust. Identities = 148/494 (29%), Positives = 251/494 (50%), Gaps = 17/494 (3%) Query: 35 LMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLG-LRVLL 93 +A LD +I N S+ TI G +GAS ++GT++ T+Y VAE + +P++ WL G +RV + Sbjct: 43 FIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFI 102 Query: 94 LTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAM 153 L+ +F LFS LCG++ +L M+I RV QG GG L+P + T++ P+ + P + Sbjct: 103 LSMV-MFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGL 161 Query: 154 FGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKFEQLAEA 213 + T ++ P+ GP++GG L + SW Y F+IN+PV + + K +L Sbjct: 162 WAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKL-PI 220 Query: 214 DWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHPVIQ 273 D G+ + + + L ++L+EG+ + WF+S +I LA T+V+ FI I ++ R+P++ Sbjct: 221 DRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVD 280 Query: 274 LRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMM 333 LR+ R R F + V + A +G+ + P +L G GY + SG I L+G+ ++ Sbjct: 281 LRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTA 340 Query: 334 PFTPLLMRLVDIRIAVGTGFLLLA----ASAFIETDLSPLSYGGSFVASQLMRGVGTVLA 389 P + +D R V G L AF TD+S G ++G+G L Sbjct: 341 PMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPL----FLQGIGLPLF 396 Query: 390 MLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESLNA 449 + L A+ SV + A+GL S R + G++A + + + L ++ Sbjct: 397 FVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDR 456 Query: 450 NSDLVQSSMTAQGHLLGGQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLVLFL 509 D + +M A G + A + T+Q+QA+ ++ +F M V+ + L ++L Sbjct: 457 GGDAIH-TMMASGM---SAEQATASLAQTVQNQAVMLSTNHIFMMAAVS-FSLAALAVWL 511 Query: 510 RPLPTHAAPV-ASH 522 P PT A A+H Sbjct: 512 APKPTRVADTSAAH 525 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 117 bits (294), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 109/397 (27%), Positives = 184/397 (46%), Gaps = 24/397 (6%) Query: 33 GALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLRVL 92 G M TLD +IVN++LP + +G + AY + + P S WL G R + Sbjct: 61 GFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRM 120 Query: 93 LLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNA 152 A LF+L S+LC ++++LPM++ RV QG G L+P + +P +Q A Sbjct: 121 YFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALA 180 Query: 153 MFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYER----PKFE 208 LGPV+GPV+GGWL E++SWH+ F INLPVG + +L +P + P+F Sbjct: 181 SISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRF- 239 Query: 209 QLAEADWFGIAGLALGLGGLTVVLE---EGEREQWFSSPDIRWLAATSVLGFILLAIGQV 265 D G L+ + ++ L+ + R W S + +AA +VLG+ A Sbjct: 240 -----DLLGCVLLSACMVAFSLALDVPFDSHRTGW--SIALFVVAALTVLGYWRHASVHP 292 Query: 266 RARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLS 325 R P+ +L L + F + ++ + +++P L GY+ L +G ++L + Sbjct: 293 R---PLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPA 349 Query: 326 GVPMILMMPF-TPLLMRL-VDIRIAVGTGFLLLAASAF-IETDLSPLSYGGSFVASQLMR 382 + + P+ PL+ R D + V T + A +F + T PL+ + + Sbjct: 350 AIAGTIAKPWIAPLVKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAA---IVQLAIF 406 Query: 383 GVGTVLAMLFLNQAAIRSVPPSQAGDASGLYSAARNL 419 G + +N ++ + AG + L+S + L Sbjct: 407 GASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQML 443 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 113/434 (26%), Positives = 204/434 (47%), Gaps = 37/434 (8%) Query: 35 LMATLDISIVNSSLPTIQGEIGASGTE-GTWIATAYLVAEIVIIPMSAWLERLLGLRVLL 93 ++ LD +IV ++LPTI E+ G E W+ATAYL+ ++ +P+ L G + + Sbjct: 32 MLVALDQTIVGTALPTIVAEL--KGFELYAWVATAYLLTSVITVPIFGRLGDYFGRKPFV 89 Query: 94 LTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQ-----QP 148 + + +F S LCG++ ++ +++ R QG GG L+ T +A P + Q Sbjct: 90 IASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPDPRVRLRWQI 149 Query: 149 VGNAMFG-ATAILGPVLGPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERP-K 206 + + FG ATA+ GP +GG+LT+ + W + F+ NLP+G + + +P+ R + Sbjct: 150 ILSVSFGIATAV-----GPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMQ 204 Query: 207 FEQLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVR 266 DW G ++L LG L +++E + S + L A SV F L + R Sbjct: 205 HAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSGMLV--LLAVSVAAFYALWKWEQR 262 Query: 267 ARHPVIQLRLLRDRQFGSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSG 326 A P++ +LRDR S+ +++++ G A++ P G G + ++G ++ Sbjct: 263 AAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAGLLI---- 318 Query: 327 VPMILMMPFTP-----LLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGSFVASQLM 381 P++ + ++ R+ + + + GF L+AAS ++ G +A L Sbjct: 319 TPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGIVLCDRVTGKGFLLAVMLA 378 Query: 382 RGVGTVLAM----LFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLW 437 G G L + +F QAA R G A+ L + R +GG L A + Q Sbjct: 379 GGFGLGLVLPNLTVFAQQAAAR----EHLGIATALLQSLRMIGGMLGTAITGTLVSQ--- 431 Query: 438 LHSRRLEESLNANS 451 ++ +E++L + Sbjct: 432 MYGAGVEKALEGDH 445 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,477,044 Number of extensions: 61248 Number of successful extensions: 383 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 360 Number of HSP's successfully gapped: 15 Length of query: 522 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 432 Effective length of database: 1,193,720 Effective search space: 515687040 Effective search space used: 515687040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 133 (55.8 bits)