BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0133.p (491 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbasp... 956 0.0 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 175 6e-45 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 165 8e-42 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 156 3e-39 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 154 1e-38 >gi|300312485|ref|YP_003776577.1| outer membrane protein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 956 bits (2472), Expect = 0.0, Method: Compositional matrix adjust. Identities = 488/491 (99%), Positives = 489/491 (99%) Query: 1 MINMDKTSSSFPAARLIALAAAALLAGCALGPDYAGPPEAAPQAAHSGQFVRGGDSATTT 60 MINMDKTSSSFPAARLIALAAAALLAGCALGPDYAGPPEAAPQAAH GQFVRGGDSATTT Sbjct: 1 MINMDKTSSSFPAARLIALAAAALLAGCALGPDYAGPPEAAPQAAHGGQFVRGGDSATTT 60 Query: 61 APINQWWQGLGDAQLTSLIERALQANPNLGIARARLQQSRAGLALEQANAAPGVGASALA 120 APINQWWQGLGDAQLTSLIERALQANPNLGIARARLQQSRAGLALEQANAAP VGASALA Sbjct: 61 APINQWWQGLGDAQLTSLIERALQANPNLGIARARLQQSRAGLALEQANAAPSVGASALA 120 Query: 121 GHARLPPANLGALTGGASSGNGSPTSANLYSLGFDASWEIDLFGGRRRAVQAATATADAA 180 GHARLPPANLGALTGGASSGNGSPTSANLYSLGFDASWEIDLFGGRRRAVQAATATADAA Sbjct: 121 GHARLPPANLGALTGGASSGNGSPTSANLYSLGFDASWEIDLFGGRRRAVQAATATADAA 180 Query: 181 QASLADVQVSLAAEVANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQ 240 QASLADVQVSLAAEVANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQ Sbjct: 181 QASLADVQVSLAAEVANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQ 240 Query: 241 LRIQNQLDASRADLVPLQADRDAYLNQLATLLGQEPGTLDGELAATVPVPLPPQQVAVGD 300 LRIQNQLDASRADLVPLQADRDAYLNQLATLLGQEPGTLDGELAATVPVPLPPQQVAVGD Sbjct: 241 LRIQNQLDASRADLVPLQADRDAYLNQLATLLGQEPGTLDGELAATVPVPLPPQQVAVGD 300 Query: 301 PVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAA 360 PVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAA Sbjct: 301 PVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAA 360 Query: 361 LGAPMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVA 420 LGAPMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVA Sbjct: 361 LGAPMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVA 420 Query: 421 TIARTKVSADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQK 480 TIARTK+SADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQK Sbjct: 421 TIARTKLSADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQK 480 Query: 481 ALGLGWSDEQG 491 ALGLGWSDEQG Sbjct: 481 ALGLGWSDEQG 491 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 175 bits (444), Expect = 6e-45, Method: Compositional matrix adjust. Identities = 159/495 (32%), Positives = 242/495 (48%), Gaps = 42/495 (8%) Query: 16 LIALAAAALLAGCALGPDYAGPPEAAPQAAH----SGQFVRGGDSATTTAPINQWWQGLG 71 ++ + A LL GC L PDY P AP AAH + G SA A I W Sbjct: 15 MLPVMLAMLLGGCTLMPDYHRPD--APVAAHFAGADSKNAAGASSAPVVADIG-WRDVFT 71 Query: 72 DAQLTSLIERALQANPNLGIARARLQQSRAGLALEQANAAPGVGASALAGHARLPPANLG 131 D L +I AL+ N +L +A ++++RA +++A P + A+ +R P Sbjct: 72 DPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRTP----- 126 Query: 132 ALTGGASSGNGSPTSANLYS--LGFDASWEIDLFGGRR----RAVQAATATADAAQASLA 185 G S GS ++ Y+ LGF A +E+DLFG R +A+Q+ +TA+A +++ Sbjct: 127 ----GDLSSTGSALTSRSYTATLGFSA-YELDLFGRVRSLKEQALQSFLSTAEARRST-- 179 Query: 186 DVQVSLAAEVANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQLRIQN 245 Q+SL AEVA A+++L Q RL L Q +++Q LT + E G++S+L + Q Sbjct: 180 --QISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQT 237 Query: 246 QLDASRADLVPLQADRDAYLNQLATLLGQE-------PGTLDGELAATVPV-PLPPQQVA 297 ++++R D+ A D N L L+G++ G D LAA P+ +PP Sbjct: 238 SVESARVDVESYTAQVDQDRNALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPP---- 293 Query: 298 VGDPVSLLRRRPDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDD 357 G P LL+RR D+ AER L A A IG A AA YPS+ L G + L + Sbjct: 294 -GLPSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGS 352 Query: 358 FAALGAPMLSWNFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALS-RFRYGR 416 + P +S D G +A + A RD + +Y +++ A R+ DAL+ R GR Sbjct: 353 GSWSFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGR 412 Query: 417 VSVATIARTKVSADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFV 476 A A + S D A L++ RY +G + + VLD+ R +AQ+NL+ + + + V Sbjct: 413 QLQAQEALVQAS-DEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLV 471 Query: 477 GIQKALGLGWSDEQG 491 K LG GW + G Sbjct: 472 TFYKTLGGGWVERTG 486 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 165 bits (417), Expect = 8e-42, Method: Compositional matrix adjust. Identities = 161/479 (33%), Positives = 229/479 (47%), Gaps = 46/479 (9%) Query: 27 GCALGPDYAGPPEAAPQAAHSGQFVRGGDSATTTAPINQWWQGLGDAQLTSLIERALQAN 86 GCA GPDY P P A S R A T P WW+ GDAQL +L ++AL N Sbjct: 18 GCASGPDYTRPQVDMPAAWTSEAPWRSMQPADAT-PRGPWWERFGDAQLNALQQQALAGN 76 Query: 87 PNLGIARARLQQSRAGLALEQANAAPGVGASALAGHARL----PPANLGALTGGASSGNG 142 L IA ARL Q+RA L + A P V +A A +R+ P N +S N Sbjct: 77 QTLAIATARLAQARAQLNVTGAAQTPQVNLNARAARSRISANRPLTNY-------NSPNF 129 Query: 143 SPTSANLYSLGFDASWEIDLFGGRRRAVQAATATADAAQASLADVQVSLAAEVANAYISL 202 S T N ++LG S+E+D FG +R+V+AA A A+ + A L + ++ L AE+A Y +L Sbjct: 130 S-TVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQSAADLENTRLLLTAELATNYFNL 188 Query: 203 RDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQLRIQNQLDASRADLVPLQADRD 262 R+ L + ++A+ Q Q L+L R + G S LD + Q LD++ + L R Sbjct: 189 RELDVELDVVRRAIALQRQALELATARHDLGATSGLDMAQQQALLDSTLTQIDLLGRQRA 248 Query: 263 AYLNQLATLLGQEPGTLDGELAATVPVPLPPQQVAVGDPVSLLRRRPDVRAAERTLAADT 322 Y + +ATL G + T P+ P + VG P +L RRPD+ AAER +AA Sbjct: 249 QYEHAIATLTGTPAPAFSITPSLT---PITPPAIPVGVPSDVLERRPDIAAAERAMAAAN 305 Query: 323 AKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAALGAPMLSW--------NFLDFG 374 A+IG A AA YPS L G+ + + L AP L W + D G Sbjct: 306 AQIGVASAAYYPSFMLQPSYGVDSRNWATL--------FNAPSLLWSLGVSASQSLFDGG 357 Query: 375 RAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVATIARTKVSADRALA 434 R +A V ++ + YR++VL A+++ ED ++ + A SA R L Sbjct: 358 RLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLE 417 Query: 435 LARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSF-------VGIQKALGLGW 486 +A RY+ G ++V ITAQQ+LL +E + V + KALG W Sbjct: 418 IASSRYEGGATPYLDV-------ITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDW 469 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 156 bits (395), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 149/475 (31%), Positives = 225/475 (47%), Gaps = 26/475 (5%) Query: 27 GCALGPDYAGP----PEAAPQAAHSGQFVRGGDSATTTAPIN-----QWWQGLGDAQLTS 77 GC++ P Y P P A +AA + Q G T P +WW+ GD +L + Sbjct: 30 GCSVAPTYERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNA 89 Query: 78 LIERALQANPNLGIARARLQQSRAGLALEQANAAPGVGASALAGHARLPPANLGALTGGA 137 L + A QAN +L A ARL Q+RA +A P V A A R PA+ G Sbjct: 90 LEDTAQQANQDLKAAAARLGQARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADAD 149 Query: 138 SSGNGSPTSANLYSLGFDASWEIDLFGGRRRAVQAATATADAAQASLADVQVSLAAEVAN 197 + N LY + S+E+DLFG V AATA A ++A V ++L A+VA Sbjct: 150 TRPN------TLYRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQ 203 Query: 198 AYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQLRIQNQLDASRADLVPL 257 Y +R+ AL + V +++ L+L +R + G S +D R + +L +++++ + + Sbjct: 204 QYFLVRELDAAAALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQSEALGI 263 Query: 258 QADRDAYLNQLATLLGQEPG--TLDGELAATVPVPLPPQQVAVGDPVSLLRRRPDVRAAE 315 R + LA LLG+ P +L + + V +PP G P +LL RRPD+ AAE Sbjct: 264 ARQRATAEHALAILLGRTPAEFSLPPQPLTRLAVTVPP-----GLPSALLERRPDIAAAE 318 Query: 316 RTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAALGAPM----LSWNFL 371 R +AA A++G A++A +PSL L G +G DL L P+ LS Sbjct: 319 RAMAAANARVGVAKSAFFPSLNLTGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLF 378 Query: 372 DFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDALSRFRYGRVSVATIARTKVSADR 431 D GR +A V +A +E YR +VL A R+ ED L+ R ++ R Sbjct: 379 DGGRRQAGVDRARASYEEQVASYRGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASAR 438 Query: 432 ALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGLGW 486 A L+ +Y+ G + V+D R + Q+ + + RS V + +ALG GW Sbjct: 439 AAKLSHTQYREGAVSYFSVIDADRTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 154 bits (390), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 139/481 (28%), Positives = 223/481 (46%), Gaps = 34/481 (7%) Query: 18 ALAAAALLAGCALGPDYAGPPEAAPQAAHSGQFVRGGDSATTTAPINQ-WWQGLGDAQLT 76 AL A +L GC+L P Y PQA + +N W Q D +L Sbjct: 18 ALVMAGVLGGCSLAPTYE-----RPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQRLQ 72 Query: 77 SLIERALQANPNLGIARARLQQSRAGLALEQANAAPGVGASALAGHARLPPANLGALTGG 136 LI AL+ N +L A ++++RA + +A+ P + S A R T Sbjct: 73 QLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTR---------TAA 123 Query: 137 ASSGNGSPTSANLYSLGFDA-SWEIDLFGGRRRAVQAATATADAAQASLADVQVSLAAEV 195 + S G PT +N Y +G ++E+D FG R AA A+ + + + Q+SL A+V Sbjct: 124 SQSLTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQV 183 Query: 196 ANAYISLRDRQQRLALGQQAVQAQEQMLQLTGQRVERGTASSLDQLRIQNQLDASRADLV 255 A AY++ R ++L L + +++++ L L +R++ G +++LD Q ++++R Sbjct: 184 AQAYLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKT 243 Query: 256 PLQADRDAYLNQLATLLGQEPGTL-------DGELAATVPVPLPPQQVAVGDPVSLLRRR 308 L+ R N L L+G++ G L D ++ +P G P LL R Sbjct: 244 QLERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDIPA---------GLPSDLLTNR 294 Query: 309 PDVRAAERTLAADTAKIGQAEAARYPSLKLFGVIGLGGTHPSDLTRLDDFAALGAPMLSW 368 PD+R+AE++L A A IG A AA +P + L G G + L A P LS Sbjct: 295 PDIRSAEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSL 354 Query: 369 NFLDFGRAKARVTQAERVRDEAEMKYRQSVLEALRDAEDAL-SRFRYGRVSVATIARTKV 427 DFGR A + AE +++A + Y +++ A R+ DAL +R A A Sbjct: 355 PIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAA 414 Query: 428 SADRALALARQRYQAGTGTLIEVLDTTRQQITAQQNLLQAEANLTRSFVGIQKALGLGWS 487 +R L L R++ G + ++ LD R +AQQ L+QA + + + KALG G + Sbjct: 415 QQER-LKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLN 473 Query: 488 D 488 + Sbjct: 474 E 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.129 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,273,588 Number of extensions: 50072 Number of successful extensions: 333 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 271 Number of HSP's successfully gapped: 18 Length of query: 491 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 402 Effective length of database: 1,198,455 Effective search space: 481778910 Effective search space used: 481778910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)