BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0122.p (399 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoac... 818 0.0 gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum ... 368 e-103 gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspiri... 342 4e-95 >gi|300312475|ref|YP_003776567.1| metal-dependent amidase/aminoacylase/carboxypeptidase [Herbaspirillum seropedicae SmR1] Length = 399 Score = 818 bits (2112), Expect = 0.0, Method: Compositional matrix adjust. Identities = 397/399 (99%), Positives = 398/399 (99%) Query: 1 MSHAHPGHLCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRH 60 MSHAHPGHLCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRH Sbjct: 1 MSHAHPGHLCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRH 60 Query: 61 IGGNGLVATLRVGSSTRSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLG 120 IGGNGLVATLRVGSS RSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLG Sbjct: 61 IGGNGLVATLRVGSSARSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLG 120 Query: 121 AARHLARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAG 180 AARHLARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAG Sbjct: 121 AARHLARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAG 180 Query: 181 TFMFRSGPFMAACDTVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDS 240 TFMFRSGPFMAACDTVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDS Sbjct: 181 TFMFRSGPFMAACDTVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDS 240 Query: 241 AVVTVGSLHAGKASNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEID 300 AVVTVGSLHAGKASNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEID Sbjct: 241 AVVTVGSLHAGKASNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEID 300 Query: 301 YLRGYPVLVNSDAETDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGN 360 YLRGYPVLVNSDAETDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFL+QRPGCFLRVGN Sbjct: 301 YLRGYPVLVNSDAETDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFLRQRPGCFLRVGN 360 Query: 361 GQGKPMLHNAGYDFNDANIPIGAAYWTRLVERWMPLQGE 399 GQGKPMLHNAGYDFNDANIPIGAAYWTRLVERWMPLQGE Sbjct: 361 GQGKPMLHNAGYDFNDANIPIGAAYWTRLVERWMPLQGE 399 >gi|300310161|ref|YP_003774253.1| aminohydrolase [Herbaspirillum seropedicae SmR1] Length = 402 Score = 368 bits (945), Expect = e-103, Method: Compositional matrix adjust. Identities = 189/385 (49%), Positives = 246/385 (63%), Gaps = 13/385 (3%) Query: 21 EITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGNGLVATLRVGSSTRSIG 80 ++ +IRRHLH HPEL FEE TA+ VA L +G V +IGG G+VATLR G STR+I Sbjct: 16 DLVSIRRHLHAHPELRFEEKRTASFVADMLRSYGLTVAENIGGYGVVATLRKGKSTRAIA 75 Query: 81 LRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARHLARTRRFDGTLNLIFQ 140 LRADMDALP+ E+ S G MHACGHDGHT MLLGAAR L+R FDGT++ +FQ Sbjct: 76 LRADMDALPMSEQNDFSHISTCAGKMHACGHDGHTTMLLGAARRLSREVEFDGTVHFVFQ 135 Query: 141 PAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMFRSGPFMAACDTVKIRI 200 PAEE G +GA+ M+ DGLFERFP +A+FG+HN PG+ AG+F R GP MA+ +T + Sbjct: 136 PAEEGG--AGARLMIEDGLFERFPADAIFGVHNWPGLPAGSFGLRPGPLMASSNTFSATL 193 Query: 201 TGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVTVGSLHAGKASNVIPEF 260 GRG+H A+PH S+DPV+ AA L +A Q++V+RNI+P AV++V LH G A NVIPE Sbjct: 194 FGRGAHGAQPHRSIDPVLAAAQLTLAWQSIVTRNINPNHRAVISVTQLHTGTADNVIPEQ 253 Query: 261 ATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRGYPVLVNSDAETDFARS 320 AT+ +VRSF E +L+E R+ A+ Q + + + + R YP +VN T A Sbjct: 254 ATLSGTVRSFDAETLDLIEHRMAAIAEAIGQMFDLRVQFHFERLYPAVVNHPEATQQAMQ 313 Query: 321 VAEELVGPEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNG---QGKPM--------LHN 369 VG E+V P SEDFA++LQ +PGC+ +GNG GK LH+ Sbjct: 314 AMMAAVGAEQVDGDTEPAFASEDFAFYLQHKPGCYAFLGNGVIADGKQAGSQATSRELHS 373 Query: 370 AGYDFNDANIPIGAAYWTRLVERWM 394 YDFND I G AYW LV +++ Sbjct: 374 PFYDFNDDIIDAGVAYWVSLVRQYL 398 >gi|300310472|ref|YP_003774564.1| hippurate hydrolase [Herbaspirillum seropedicae SmR1] Length = 403 Score = 342 bits (876), Expect = 4e-95, Method: Compositional matrix adjust. Identities = 178/400 (44%), Positives = 249/400 (62%), Gaps = 9/400 (2%) Query: 5 HPGHLCEVGDLHDIQDEITAIRRHLHQHPELSFEEVDTAALVAQRLEQWGYAVTRHIGGN 64 H GH + LH + +RR LH HPEL FEE TA +V + L+ GY VTR +GG Sbjct: 3 HTGHQHDT--LHQHRAHWAGLRRDLHAHPELRFEEHRTADVVVRELQSLGYTVTRGLGGT 60 Query: 65 GLVATLRVGSSTRSIGLRADMDALPIQEETGLDWASVKPGAMHACGHDGHTAMLLGAARH 124 G+VA+L R I LRAD+DALPI E AS G MHACGHDGHT MLLGAAR Sbjct: 61 GVVASLAGADPQRGIVLRADLDALPIVEANDFAHASCTHGVMHACGHDGHTVMLLGAARL 120 Query: 125 LARTRRFDGTLNLIFQPAEEAGFDSGAQKMLADGLFERFPCEAVFGIHNHPGVEAGTFMF 184 L + G+++ +FQP EE G +GA+KM+ DGLFE+FP EAVFG+HN PG+ AG F Sbjct: 121 LKQGPMLPGSVHFVFQPGEEGG--AGARKMIDDGLFEQFPTEAVFGMHNWPGLPAGQFGL 178 Query: 185 RSGPFMAACDTVKIRITGRGSHAARPHLSVDPVVVAASLVMALQTVVSRNIDPMDSAVVT 244 R GP MAA +KI ITG+G+HAA+PHL +DP+ +A S+V+ QT+ +R+ DP+D AV++ Sbjct: 179 RPGPIMAAGSRLKITITGKGAHAAQPHLGLDPIPLACSMVLQCQTIAARHKDPVDPAVIS 238 Query: 245 VGSLHAGKASNVIPEFATMELSVRSFKPEVRELLEQRIRALVSTHAQSYGAQAEIDYLRG 304 V HAG NVIP+ A + ++R+ +++ L++ IR + A++YGA+ E+++ + Sbjct: 239 VCMFHAGDTDNVIPDRAELRGTIRTLSSTLQQKLQEDIRRMCHALAEAYGAKVEVEFFQY 298 Query: 305 YPVLVNSDAETDFARSVAEELVGPEKVIAPFGPIAGSEDFAYFLQQRPGCFLRVGNG--- 361 YP +N+ AETDF V + G +++ SEDF + L++RPG ++ +GN Sbjct: 299 YPATINTPAETDFCERVIRQTFGDDRIRTGIPANMTSEDFGFMLEERPGAYVLIGNAPQD 358 Query: 362 QGKPMLHNAGYDFNDANIPIGAAYWTRLVERWM--PLQGE 399 + LH+ YDFND I G YW L + + L+GE Sbjct: 359 RASHSLHHPHYDFNDDIIEAGVRYWVALAQGYFGSGLKGE 398 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,362,020 Number of extensions: 60564 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 168 Number of HSP's successfully gapped: 3 Length of query: 399 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 312 Effective length of database: 1,207,925 Effective search space: 376872600 Effective search space used: 376872600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)