BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0071.p (427 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 841 0.0 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 387 e-108 gi|300309357|ref|YP_003773449.1| major facilitator superfamily (... 367 e-102 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 301 8e-83 gi|300310160|ref|YP_003774252.1| major facilitator superfamily p... 296 3e-81 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 841 bits (2172), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/427 (99%), Positives = 427/427 (100%) Query: 1 MSSNTASPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL 60 MSSNTA+PGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL Sbjct: 1 MSSNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLL 60 Query: 61 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLA 120 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLA Sbjct: 61 LTFATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLA 120 Query: 121 PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLN 180 PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLN Sbjct: 121 PAGIALGKMIQGFSAGGEFGSSTAFLVEHAPHRRGFFSSWQVASQGISLLLAALFGAVLN 180 Query: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM 240 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM Sbjct: 181 NMLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM 240 Query: 241 RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD 300 RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD Sbjct: 241 RLLIGAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSD 300 Query: 301 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI 360 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI Sbjct: 301 KIGRTPFMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADI 360 Query: 361 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSA 420 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSA Sbjct: 361 FPVATRGTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSA 420 Query: 421 RLVLRLR 427 RLVLRLR Sbjct: 421 RLVLRLR 427 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 387 bits (993), Expect = e-108, Method: Compositional matrix adjust. Identities = 199/413 (48%), Positives = 276/413 (66%), Gaps = 2/413 (0%) Query: 11 DITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSF 70 +++ Q RRAI+++ IGN LEWFD +Y FAV+IAK FFPTG+D SLLL ATFGV F Sbjct: 13 SVSNQQRRRAIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGF 72 Query: 71 FMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMI 130 FMRP+G ++LG Y+DR GRKAAL ++I+LM VGT MI P+Y SIGL AP I + +++ Sbjct: 73 FMRPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLL 132 Query: 131 QGFSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLA 189 QGFSAGGE GS+TAFL E+AP R R ++SSW AS G+++LL A G + L LA Sbjct: 133 QGFSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALA 192 Query: 190 SWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMRLLIGAGSV 249 SWGWR+PF+ G++I P G YIR HL+E P F E +KT++PL++ + V Sbjct: 193 SWGWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMV 252 Query: 250 IMATVSVYLSL-YIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFM 308 I+ TV Y+ L Y+PTY+VK L LP + F A V GL + + +P+VG L+D+IGR P + Sbjct: 253 ILWTVCTYVLLFYMPTYSVKVLKLPQTTGFIAGMVGGLCIMVLAPIVGRLADRIGRRPLL 312 Query: 309 ITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGT 368 S+AL ++L YPMF ++ ++P LL+ Q V G L+ Y + A +++FP T Sbjct: 313 SGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAKVLST 372 Query: 369 GMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 G+S+AYN+AVTIFGGFA ITWLI TG ++ +++V+ A +S + + R Sbjct: 373 GLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVR 425 >gi|300309357|ref|YP_003773449.1| major facilitator superfamily (MFS) transporter protein [Herbaspirillum seropedicae SmR1] Length = 437 Score = 367 bits (941), Expect = e-102, Method: Compositional matrix adjust. Identities = 181/415 (43%), Positives = 280/415 (67%), Gaps = 7/415 (1%) Query: 14 HPQ--ARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFF 71 PQ +R +I++ +GNALEW+D ++YG F+ +IA+ FFP ++ SLL+ ATFG+ FF Sbjct: 2 QPQLNKQRQVIAAIVGNALEWYDFIVYGFFSAIIARLFFPADNEYTSLLVALATFGIGFF 61 Query: 72 MRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQ 131 MRP+G V+LG Y+DR GRKAA+ + I+LMT+ A+IAF P+YA+IG+ AP I + +M+Q Sbjct: 62 MRPVGGVLLGLYADRKGRKAAMQVIIVLMTLAIAIIAFTPTYATIGIAAPIMIVVARMLQ 121 Query: 132 GFSAGGEFGSSTAFLVEHAP-HRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLAS 190 GF+ GGE+ SSTAFLVE AP H+RG + SWQ+ Q +++ A GA++ L+PE L S Sbjct: 122 GFATGGEYSSSTAFLVESAPAHKRGLYGSWQLVGQCLAVFCGAGMGALVTRNLSPEALDS 181 Query: 191 WGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTN-APLR--DTFAHQKMRLLIGAG 247 WGWR+PF+ GLLI P G++IRRH+ E +F ES+ K N AP++ D +L+ G Sbjct: 182 WGWRLPFVLGLLIGPVGLWIRRHMHETDDFLESANKPNAAPIKLLDVVKTNLRGVLVSMG 241 Query: 248 SVIMATVSVYLSLY-IPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTP 306 VI TV+ Y+ L +PT+A KQLGLP +F +A ++ P GALSD++GR Sbjct: 242 QVINGTVAFYVVLVNMPTFANKQLGLPLDQAFMVQMIAVALLTAVIPFAGALSDRLGRRT 301 Query: 307 FMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATR 366 ++ SS ++++ YP+FV+++ +P +LL++Q ++ +++ F MP L++ FP R Sbjct: 302 VLLYSSLAFLLMVYPLFVWVSAAPSLPRLLVMQVLLCIVIGANFGPMPTALSEQFPTRVR 361 Query: 367 GTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASLSAR 421 TG+++AYN+A +FGGFA I+TWL +G ++ +++V+F A + + A+ + R Sbjct: 362 STGLAVAYNLAAMLFGGFAPFIVTWLTKTSGSPVAPAWYVLFAACIGVSAAWAMR 416 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 301 bits (770), Expect = 8e-83, Method: Compositional matrix adjust. Identities = 165/396 (41%), Positives = 241/396 (60%), Gaps = 5/396 (1%) Query: 27 GNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFFMRPLGAVVLGAYSDR 86 GN +E FD ++G +A I+K FFP+ ++ SL+LTF TFG F MRPLGA++LGAY DR Sbjct: 20 GNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRPLGAIILGAYVDR 79 Query: 87 AGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQGFSAGGEFGSSTAFL 146 GR+ L++++ LM +GT +IAF+PSYA+IG LAP + +G+++QGFSAG E G + +L Sbjct: 80 VGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFSAGVELGGVSVYL 139 Query: 147 VEHA-PHRRGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLASWGWRVPFIFGLLIAP 205 E A P +GF+ SWQ ASQ +++++AA G +L+ LTP +++ WGWR+PF G LI P Sbjct: 140 AEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGWRIPFFIGCLIVP 199 Query: 206 AGIYIRRHLDEAPEFKESSEK--TNAPLRDTFAHQKMRLLIGAGSVIMATVSVYL-SLYI 262 IRR L E EF + T R + K+ ++ G V M TVS YL ++Y Sbjct: 200 VLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKL-VIAGMMLVSMTTVSFYLITVYT 258 Query: 263 PTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTPFMITSSALYIVLTYPM 322 PT+ L L +S GL FI P++GALSD++GR P ++T + L I+ YP Sbjct: 259 PTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTFTVLTILTAYPA 318 Query: 323 FVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATRGTGMSLAYNIAVTIFG 382 +L + F ++L+++ + L Y AM L ++ P R G SLAY++A +FG Sbjct: 319 VNWLVHDASFAKMLIVELWLSFLYASYNGAMVVALTEVMPADVRTAGFSLAYSLATAVFG 378 Query: 383 GFAGLIITWLIDFTGDKLSVSYFVIFGAVLSLIASL 418 GF I T LI++T DK + ++ AV LIA+L Sbjct: 379 GFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATL 414 >gi|300310160|ref|YP_003774252.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 428 Score = 296 bits (757), Expect = 3e-81, Method: Compositional matrix adjust. Identities = 170/394 (43%), Positives = 243/394 (61%), Gaps = 9/394 (2%) Query: 13 THPQA-RRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLTFATFGVSFF 71 T P+ RRA+I++++GN LE +D +I+G FAV I+K FFP+GD+ +L+ TF TF F Sbjct: 8 TDPKTQRRAVIAATLGNVLEIYDFIIFGIFAVAISKTFFPSGDEYAALMFTFMTFAAGFV 67 Query: 72 MRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPAGIALGKMIQ 131 +RP+GA+VLG Y+D+ GRK AL +++LM +GT + A PSY SIG+ A + G+++Q Sbjct: 68 VRPIGALVLGQYADKVGRKKALSFTLILMAIGTVIPALCPSYDSIGIAATVILVFGRLLQ 127 Query: 132 GFSAGGEFGSSTAFLVEHAPHR-RGFFSSWQVASQGISLLLAALFGAVLNNMLTPEQLAS 190 GFSAGGE GS+ A LVE+A R R F S+Q +QG +LL + G V+ + E++ Sbjct: 128 GFSAGGEIGSNVAMLVENASSRNRALFCSFQQMAQGGGVLLGGIVGYVIAVVYGQERMLQ 187 Query: 191 WGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKM----RLLIGA 246 WR+ F GL+I P G YIRR L E PEF+ S +K + D+F R+LIG Sbjct: 188 DYWRLAFFVGLVIGPVGWYIRRVLPETPEFERSKKKESV---DSFLEGVKANFGRILIGI 244 Query: 247 GSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGALSDKIGRTP 306 +I TV+ +S Y TYAV++L +P S++ +GL + PLVG L+D+IG Sbjct: 245 AIMIFWTVATQVSNYFTTYAVRELHMPVASTYLGQISSGLTLLAICPLVGMLADRIGPYK 304 Query: 307 FMITSSALYIVLTYPMFVFLTNSPGFLQLLLLQTVIGVLMTMYFAAMPALLADIFPVATR 366 M +AL VL YP+F L +P LLL+Q I V + +Y A P +++DIFP R Sbjct: 305 PMAFGAALAGVLAYPLFKMLVATPTLPTLLLVQIAISVTLGLYAACAPLVMSDIFPTRYR 364 Query: 367 GTGMSLAYNIAVTIFGGFAGLIITWLIDFTGDKL 400 TG+ +Y + V IFGG +I+T LI +GDKL Sbjct: 365 ATGIGTSYALGVMIFGGLTPMIVTTLIQISGDKL 398 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,311,713 Number of extensions: 58044 Number of successful extensions: 383 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 329 Number of HSP's successfully gapped: 18 Length of query: 427 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 339 Effective length of database: 1,203,190 Effective search space: 407881410 Effective search space used: 407881410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)