BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0064.p (628 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 1283 0.0 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 428 e-121 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 167 2e-42 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 163 3e-41 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 139 5e-34 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 1283 bits (3321), Expect = 0.0, Method: Compositional matrix adjust. Identities = 623/628 (99%), Positives = 625/628 (99%) Query: 1 MQYEQFYRQSVEQPEAFWAQEAQRIHWQQPFSRTLDYSRPPFASWFIDGQTNLCHNAVDR 60 MQYEQFYRQSVEQPEAFWAQEAQRIHWQQPFSRTLDYSRPPFA WFIDGQTNLCHNAVDR Sbjct: 1 MQYEQFYRQSVEQPEAFWAQEAQRIHWQQPFSRTLDYSRPPFARWFIDGQTNLCHNAVDR 60 Query: 61 HLPERAQQAALIAISTETNSERIYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 HLPERAQQAALIAISTETNSER+YTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE Sbjct: 61 HLPERAQQAALIAISTETNSERVYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 Query: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE Sbjct: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 Query: 181 AIAMAQHQPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQHVPVTWLESNASSYILYT 240 AIAMAQH+PQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQ VPVTWLESNASSYILYT Sbjct: 181 AIAMAQHRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQQVPVTWLESNASSYILYT 240 Query: 241 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIAG 300 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIAG Sbjct: 241 SGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIAG 300 Query: 301 MATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMERYDTSSLKALY 360 MATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMERYDTSSLKALY Sbjct: 301 MATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMERYDTSSLKALY 360 Query: 361 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSPGVPMPGYR 420 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSPGVPMPGYR Sbjct: 361 LAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSPGVPMPGYR 420 Query: 421 LAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLPREVYSTFDWG 480 LAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLPREVYSTFDWG Sbjct: 421 LAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWRSLPREVYSTFDWG 480 Query: 481 IRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAF 540 IRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAF Sbjct: 481 IRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVEDKLKGQVAFAF 540 Query: 541 AIPKQADTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 AIPKQA TIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS Sbjct: 541 AIPKQAYTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRSGKLLRRAIQS 600 Query: 601 ICEGRDPGDLPTIEDPAALEQVRAVIKD 628 ICEGRDPGDLPTIEDPAALEQVRAVIKD Sbjct: 601 ICEGRDPGDLPTIEDPAALEQVRAVIKD 628 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 428 bits (1100), Expect = e-121, Method: Compositional matrix adjust. Identities = 245/634 (38%), Positives = 373/634 (58%), Gaps = 21/634 (3%) Query: 3 YEQFYRQSVEQPEAFWAQEAQ-RIHWQQPFSRTLDYSRPPFASWFIDGQTNLCHNAVDRH 61 Y + + E WA+ A+ + W +PF++TLD S PF WF DGQ N+ +N +D + Sbjct: 32 YHALNAEFAKDYEGTWARLAKDNLKWHKPFTQTLDESNAPFYKWFADGQVNVSYNCLDVN 91 Query: 62 LPE-RAQQAALIAISTETNSERIYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPMIAE 120 L A + A+I S + R+ +Y EL Q+V A +++LG+++GDRV+IYMPM E Sbjct: 92 LENGNADKTAVIFESDDGKVTRV-SYRELHQKVCQFANGLKSLGIKKGDRVVIYMPMSVE 150 Query: 121 AVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPYKGLLDE 180 V AM ACARIGA HSVVFGGF++ SL R+ DA +++AD RGGK +P K ++DE Sbjct: 151 GVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTADYQVRGGKQLPLKSIVDE 210 Query: 181 AIAMAQHQP-QHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQHVPVTWLESNASSYILY 239 A+ M +HV++ R A + RD+ + + P W+++ +ILY Sbjct: 211 ALDMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQSDTCEP-EWVDAEHPLFILY 269 Query: 240 TSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVYGPLIA 299 TSG+TGKPKGVQ GY + +M+ F +P + Y+CT+DIGW+ GH+YI YGPL Sbjct: 270 TSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPDDVYWCTADIGWITGHTYIAYGPLAV 329 Query: 300 GMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECM----ERYDTSS 355 G +++EG+P P+AG +W +V ++K T ++APTAIR L K ++YD SS Sbjct: 330 GATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSLIKAADADEKVHPKQYDLSS 389 Query: 356 LKALYLAGEPLDETTSSWI---SGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLGSP 412 L+ L GEP++ W G P++D +WQTE+G +IS G P GS Sbjct: 390 LRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGGHMISPLPGA--TPQVPGSC 447 Query: 413 GVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYW-RSLPR 471 +P+PG AI+DE TG G++ ++ P P ++T++ D ERF +Y+ L Sbjct: 448 TLPLPGITAAIVDE-TGNDIPNGNGGILVVKRPW-PSMIRTIWNDPERFKKSYFPEELGG 505 Query: 472 EVYSTFDWGIRDKD-GYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVGVED 530 ++Y D +R+K+ GY+ I GR DDV+NV+GHR+GT EIE ++ ++ V+E AVVG D Sbjct: 506 KIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALVANSIVAEAAVVGKPD 565 Query: 531 KLKGQVAFAFAIPKQADTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPKTRS 590 + G+ AF + K+ T ++ K + E+ V K+IG + +P + F LPKTRS Sbjct: 566 ETTGESICAFVVLKRPR--PTGDEAKQIAKELRDWVAKEIGPIAKPKEIRFGDNLPKTRS 623 Query: 591 GKLLRRAIQSICEGRD-PGDLPTIEDPAALEQVR 623 GK++RR ++ + +G D+ T+E+PA L+Q++ Sbjct: 624 GKIMRRLLRVLAKGEAITQDVSTLENPAILDQLK 657 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 167 bits (424), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 163/572 (28%), Positives = 267/572 (46%), Gaps = 65/572 (11%) Query: 45 WFIDGQTNLCHNAVDRHLPERAQQAALIAISTETNSERIYTYAELQQEVMA--MAASMQA 102 W I N+ A DR A + AI E + +Y+ Q ++++ A +++A Sbjct: 26 WQIPTHFNIAQAACDRW----ADGSGRTAIICEDADGSVTSYSYDQLKILSDRFANALRA 81 Query: 103 LGVQRGDRVLIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSA 162 GV RGDR+ IY+ E V LA ++GA+ +F F +++A R+D++ +V+ Sbjct: 82 AGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTD 141 Query: 163 DAGSRGGKV---IPYKGLLDEAIAMAQHQPQHVLLVDRGLAPMARTAQRDVDYAPLRTQH 219 +G + +P L+ + PQ DR A ++D Sbjct: 142 ASGMEKATLAGELPALRLVFCVESSDLVLPQTTDFWDR-----LHAAPAELD-------- 188 Query: 220 LGQHVPVTWLESNASSYILYTSGTTGKPKGV---QRDVGGYAVALASSMQHIFCGQPGET 276 PV L + + I+YTSGTTGK KG R + G+ + S H Q G+ Sbjct: 189 -----PVLTLADD-PAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVS--HDSFPQEGDR 240 Query: 277 YFCTSDIGWVVGHSYIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTA 336 ++ +D W+ G ++ L G+A V + DA + ++ ++++ +F PTA Sbjct: 241 FWTPADWAWIGGLLDVLLPSLYHGVAVVARR--LEKFDAAEVFGLLARHQIRNVFFPPTA 298 Query: 337 IRVLKKQPPECMERYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPII 396 +++L+ + SL+++ GE L + +W AL V + + Y QTE + Sbjct: 299 LKMLRGA--ATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVS 356 Query: 397 SIAKGIDDKPTRLGSPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYG 456 S + P+ GS G +PG+ + I+DE G+ G +AI P P ++ Sbjct: 357 SSSHCY---PSVSGSMGRAVPGHVVQIVDE-QGQVLPHGTVGNIAIRAPDPVMFLR---- 408 Query: 457 DDERFVNTYWRS--LPREVYS-----TFDWGIRDKDGYYFILGRTDDVINVAGHRLGTRE 509 YWR+ RE ++ T D G D+ GY LGR DDVI AG+R+G Sbjct: 409 --------YWRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAA 460 Query: 510 IEESISSHPNVSEVAVVGVEDKLKGQVAFAFAIPKQADTIATPEQRKALEAEIMAVVDKQ 569 IEE + HP V AVVGV+D L+ +V AF + K TP++ AL+AE+ V Q Sbjct: 461 IEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDG---VTPDE--ALKAELQQHVRAQ 515 Query: 570 IGAVGRPARVFFVSGLPKTRSGKLLRRAIQSI 601 + A P + FV+ LP T +GK++R+ ++ + Sbjct: 516 LAAHEYPRLISFVAALPTTATGKIMRKTLKEM 547 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 163 bits (413), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 155/562 (27%), Positives = 252/562 (44%), Gaps = 50/562 (8%) Query: 52 NLCHNAVDRHLPERAQQAALIAISTETNSERIYTYAELQQEVMAMAASMQALGVQRGDRV 111 N C DRH+ + AL +S ++ Y++A+++ +A ++ G+Q G V Sbjct: 38 NACVECCDRHV--KGDNIALEFVSLHGEHQQ-YSFAQVRSMAARVANLLRQQGIQPGQIV 94 Query: 112 LIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKV 171 +P E + +L R GAV+ +F F ++ R+ + LIV+ A Sbjct: 95 AGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTNVAN------ 148 Query: 172 IPYKGLLDEAIAMAQHQPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQHVPVTWLES 231 + LDE Q V V P+ D+D+ PV L S Sbjct: 149 ---RDKLDEIANCPQ-----VCTVRESNDPLRAG---DIDFRAAVDAQSDDFAPV--LRS 195 Query: 232 NASSYILY-TSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHS 290 A ++ TSGTTG KGV + A + M+ P + ++ +D GW G Sbjct: 196 GADLMLMMSTSGTTGAAKGVPVPLSALQ-AFSVYMREAVGLLPQDKFWNMADPGWAYGLY 254 Query: 291 YIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMER 350 Y + GPL G E + + + +T + APTA R++ E Sbjct: 255 YAIIGPLAIGHGITFNEAAFTVSGT---YETIRRLGITSLAGAPTAYRMMMAAGEEAAAS 311 Query: 351 YDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGIDDKPTRLG 410 L+A+ AGEPL+ W + AL VP+ D+Y QTE G +++ + + G Sbjct: 312 V-KGRLRAVSSAGEPLNAEVVRWFASALDVPIHDHYGQTELGM-VVNNHHALRHEVVP-G 368 Query: 411 SPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWR--- 467 S G MPGYR+ +LDE E G +Q G +AI+ P +Y + + YW+ Sbjct: 369 SAGFAMPGYRVVVLDENHQE-LGANQPGELAIDIARSP-----LY-----WFSGYWKQDT 417 Query: 468 -SLPREVYSTFDWGIRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVV 526 ++ YST D + +G +GR+DDVI AG+R+G ++E ++ HP V++VAV+ Sbjct: 418 PAIAHGYYSTGDNVELEPNGSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVI 477 Query: 527 GVEDKLKGQVAFAFAIPKQADTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLP 586 G+ D + ++ AF + +D + AL ++ V +++ A P + F+ LP Sbjct: 478 GLPDPERTEIVKAFVV--LSDQFKASD---ALREQLAQHVKRRLSAHAYPRAIEFLDALP 532 Query: 587 KTRSGKLLRRAIQSICEGRDPG 608 KT SGKL R ++ + R G Sbjct: 533 KTPSGKLQRFVLRKMEADRRAG 554 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 139 bits (351), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 141/556 (25%), Positives = 252/556 (45%), Gaps = 55/556 (9%) Query: 58 VDRHLPERAQQAALIAISTETNSERIYTYAELQQEVMAMAASMQALGVQRGDRVLIYMPM 117 V R P+ + ++A S+R+ + E+ V A+A ++ GV++GD++L++ Sbjct: 39 VARRFPD--EPGLILAQGAADGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRN 96 Query: 118 IAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSADAGSRGGKVIPY-KG 176 + + A ++G V V F RI + + S G V+ Y +G Sbjct: 97 NQQIFESAWAAFKMGVVW--VPTNF-------RITPPEAAYL----GQSSGACVMLYDRG 143 Query: 177 LLD--EAIAMAQHQPQHVLLVDRGLAPMARTAQRDVDYAPLRTQHLGQHVPVTWLESNAS 234 +A+ A +HV+ +A ++DY L + G ++ + Sbjct: 144 FAQYVDAVKAASPALEHVV-------ALADARAGELDYEALVREGSGAAFREAEVDYDDP 196 Query: 235 SYILYTSGTTGKPKGVQRDVGGYAVALASSMQHIFCGQPGETYFCTSDIGWVVGHSYIVY 294 + YTSGTTG PK G A + + + + PG T+ S + + H V+ Sbjct: 197 LWFFYTSGTTGHPKAGMLSHGQMAFVVTNHLADLM---PGLTHQSRSLVVAPLSHGAGVH 253 Query: 295 GPLIA--GMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTAIRVLKKQPPECMERYD 352 + G A++L + + W +VEKY+V +F+ PT +++L + E ++RYD Sbjct: 254 AVINTARGAASILLSSERLVVEEA--WQLVEKYRVDNLFTVPTIVKILVED--ESVDRYD 309 Query: 353 TSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPIISIAKGI----DDKP-T 407 SSLK + AG P+ + L ++ Y E I + + D P Sbjct: 310 HSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYGLGEVTGNITFLPPYLHFEDDAHPQA 369 Query: 408 RLGSPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYGDDERFVNTYWR 467 R+G+ G+P G ++AIL++ G+ P + G + + GP V+ + Sbjct: 370 RVGTCGMPRTGMQIAILND-DGQELAPFETGEICVRGP-------AVFMGYHNNPEANAK 421 Query: 468 SLPREVYSTFDWGIRDKDGYYFILGRTDDVINVAGHRLGTREIEESISSHPNVSEVAVVG 527 + + + T D G D+DG+ +I GR+ D+ G + REIEE++ +HP VSEVAV+G Sbjct: 422 AFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYPREIEEALLTHPAVSEVAVLG 481 Query: 528 VEDKLKGQVAFAFAIPKQADTIATPEQRKALEAEIMAVVDKQIGAVGRPARVFFVSGLPK 587 V D G+ A + K Q+ EA ++A ++ +IG P R + +PK Sbjct: 482 VPDPKWGESGLAVIVCKAG-------QQVDGEA-LLAHLESRIGKYKWPRRFIYWDSMPK 533 Query: 588 TRSGKLLRRAIQSICE 603 + GK++++ I+++ E Sbjct: 534 SGYGKIVKKQIKALLE 549 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,139,077 Number of extensions: 97527 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 12 Length of query: 628 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 537 Effective length of database: 1,188,985 Effective search space: 638484945 Effective search space used: 638484945 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)