BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0025.p (466 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase [Herba... 959 0.0 gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA synthe... 123 2e-29 gi|300312914|ref|YP_003777006.1| glutaminyl-tRNA synthetase [Her... 69 1e-12 >gi|300312381|ref|YP_003776473.1| glutamyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 959 bits (2479), Expect = 0.0, Method: Compositional matrix adjust. Identities = 465/466 (99%), Positives = 465/466 (99%) Query: 1 MTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAI 60 MTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAI Sbjct: 17 MTATQVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAI 76 Query: 61 IEGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGE 120 IEGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGE Sbjct: 77 IEGMQWLGLEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGE 136 Query: 121 KPRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVKGPITISNRELDDLVIA 180 KPRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVKGPITISNRELDDLVIA Sbjct: 137 KPRYDGTWRPEPGKTLPAIPEGRKPVVRFKNPQDGEVSWNDVVKGPITISNRELDDLVIA 196 Query: 181 RPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGA 240 RPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGA Sbjct: 197 RPDGTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGA 256 Query: 241 DGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIFSMQQFCAWFDLDHLTK 300 DGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIFSMQQFCAWFDLDHLTK Sbjct: 257 DGEKLSKRHGAVSVMDYPAQGYLPEAMLNYLARLGWSHGDDEIFSMQQFCAWFDLDHLTK 316 Query: 301 APAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVFEGAPDLAAVIALLKDRANT 360 APAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVFEGAPDLAAVIALLKDRANT Sbjct: 317 APAQFNPEKLNWLNNHYIKLADNARLAGLVRPLLEAQGAVFEGAPDLAAVIALLKDRANT 376 Query: 361 VNEIAAAALMFYHQPAPDAALVAQHITEAIKPALADFAQRVATVEWTKEAIAPMIKEVLA 420 VNEIAAAALMFY QPAPDAALVAQHITEAIKPALADFAQRVATVEWTKEAIAPMIKEVLA Sbjct: 377 VNEIAAAALMFYRQPAPDAALVAQHITEAIKPALADFAQRVATVEWTKEAIAPMIKEVLA 436 Query: 421 AHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVQARLSGYL 466 AHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVQARLSGYL Sbjct: 437 AHGIKMPQLAMPLRLIVAGQLQTPAIDAVLQLFGREAVQARLSGYL 482 >gi|300312871|ref|YP_003776963.1| glutamyl/glutaminyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 294 Score = 123 bits (309), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 94/258 (36%), Positives = 132/258 (51%), Gaps = 15/258 (5%) Query: 9 RFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAIIEGMQWLG 68 RFAPSP+G LH G AL S+ AR G ++LRIED D R+ A Q II + L Sbjct: 9 RFAPSPSGPLHAGSLVAALASYLDARVHAGRWLLRIEDIDETRTVQGAAQDIIATLAALD 68 Query: 69 LEHDEGPFYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAGEKPRYDGTW 128 + D Q +R RY+ +A+ AY C C+ +E+ R + G Y GT Sbjct: 69 MRSDGPVLVQSQRKARYQ--LARDRLGALAYPCGCSRKEIADSRVGTASDGAA-LYPGTC 125 Query: 129 RP--EPGKTLPAIPEGRKPVVRFKNP-QDGE-VSWNDVVKGPITIS-NRELDDLVIARPD 183 R PGK + R +R +P Q GE V + D GP E+ D V+ R D Sbjct: 126 RHGLAPGK------QARTLRLRVPDPGQAGELVQFEDRWLGPQAQHLAAEVGDFVLQRAD 179 Query: 184 GTPTYNFCVVVDDWDMKITHVIRGDDHVNNTPRQINILKALGGTLPEYGHVPMILGADGE 243 G Y VVVDD + +T ++RG D +++T RQI + LG P Y HVP+++ GE Sbjct: 180 GFWAYQLAVVVDDAEQGVTDIVRGADLLDSTARQIYLQGLLGYPTPRYLHVPLLMNETGE 239 Query: 244 KLSKRHGAVSV-MDYPAQ 260 K SK++GA ++ ++ P Q Sbjct: 240 KFSKQNGAQALDLNQPLQ 257 >gi|300312914|ref|YP_003777006.1| glutaminyl-tRNA synthetase [Herbaspirillum seropedicae SmR1] Length = 583 Score = 68.6 bits (166), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 43/135 (31%), Positives = 65/135 (48%), Gaps = 10/135 (7%) Query: 5 QVRTRFAPSPTGYLHLGGARTALFSWAYARRFGGTFVLRIEDTDVERSTPEAVQAIIEGM 64 QV TRF P P GYLH+G A++ ++ A + G +R +DT+ E+ E V I++ + Sbjct: 44 QVVTRFPPEPNGYLHIGHAKSICLNFGLAADYAGRCHMRFDDTNPEKEEQEYVDTILDSV 103 Query: 65 QWLGL--EHDEGP---FYQMKRMDRYREVIAQMLEQGTAYYCYCTPEEVEAMRERQRAAG 119 +WLG EH EG +Y D ++ +G A Y + E MRE R Sbjct: 104 KWLGFSWEH-EGKSHLYYASNYFDVMYACAQALIRKGLA---YVDEQNAEQMRE-NRGTL 158 Query: 120 EKPRYDGTWRPEPGK 134 +P + WR P + Sbjct: 159 TEPGKNSPWRDRPAE 173 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,655,717 Number of extensions: 75904 Number of successful extensions: 228 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 3 Length of query: 466 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 377 Effective length of database: 1,198,455 Effective search space: 451817535 Effective search space used: 451817535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)