BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0024.p (269 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine ... 535 e-153 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 119 3e-28 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 101 6e-23 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 99 5e-22 gi|300310158|ref|YP_003774250.1| enoyl-CoA hydratase [Herbaspiri... 97 1e-21 >gi|300312380|ref|YP_003776472.1| enoyl-CoA hydratase/carnithine racemase [Herbaspirillum seropedicae SmR1] Length = 269 Score = 535 bits (1379), Expect = e-153, Method: Compositional matrix adjust. Identities = 266/269 (98%), Positives = 267/269 (99%) Query: 1 MSSAAHLDITPRGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKS 60 MSSAAHLDITPRGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKS Sbjct: 1 MSSAAHLDITPRGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKS 60 Query: 61 FSAGADLAWMRRMADASREDNLRDARQLALLMETLNSFPTPTLAKVQGAVMGGGVGLVSC 120 FSAGADLAWMRRMADASREDNLRDAR+LALLMETLNSFPTPTLAKVQGAVMGGGVGLVSC Sbjct: 61 FSAGADLAWMRRMADASREDNLRDARKLALLMETLNSFPTPTLAKVQGAVMGGGVGLVSC 120 Query: 121 CDIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLV 180 CDIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLV Sbjct: 121 CDIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLV 180 Query: 181 HEVASPDELDVRTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRA 240 HEVA PDELD RTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRA Sbjct: 181 HEVAPPDELDARTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRA 240 Query: 241 SAEGREGLRAFLDKDAPAWTRQQPTSGAA 269 SAEGREGLRAFLDKDAPAWTRQQPTSGAA Sbjct: 241 SAEGREGLRAFLDKDAPAWTRQQPTSGAA 269 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 119 bits (297), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 79/258 (30%), Positives = 131/258 (50%), Gaps = 7/258 (2%) Query: 6 HLDITPR----GLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKSF 61 H DI R G+ T+TLNRP+ NA ++++ L L + D+ ++LA+ GK+F Sbjct: 5 HPDILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAF 64 Query: 62 SAGADLAWMRRMADASREDNLRDARQLALLMETLNSFPTPTLAKVQGAVMGGGVGLVSCC 121 AG DL M+ + + L + + LM++L P P +AKVQG G LV+ C Sbjct: 65 CAGHDLRQMQATPQQTYYEAL--FHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATC 122 Query: 122 DIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGLVH 181 D+ +A+++A FA+S + +GL + + + + + +A V+ + AA AAA GL++ Sbjct: 123 DLAIAAESARFAVSGINVGLFCSTPAVALTRNLPIKRAFEMLVTGKFIDAATAAAWGLIN 182 Query: 182 EVASPDELDVRTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLRAS 241 + ELD T L + + ++ K +V + P+ + A V+A Sbjct: 183 QAVPEQELDSATQALAREICSKPAISIARGKAMV-YRQQMMALPDAYAHAAEVMACNIMD 241 Query: 242 AEGREGLRAFLDKDAPAW 259 E +EG+ AFLDK P W Sbjct: 242 PEAQEGITAFLDKRKPNW 259 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 101 bits (252), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 85/270 (31%), Positives = 128/270 (47%), Gaps = 18/270 (6%) Query: 1 MSSAAHLDITPRG-LATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTG- 58 M++ +L + G A +TL+ P N + +A L L+ + D L++ G Sbjct: 1 MTTYTNLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGE 59 Query: 59 KSFSAGADLAWMRRMADASREDNLRDARQLALLMETLNSFPTPTLAKVQGAVMGGGVGLV 118 K FSAGADL + AD + AR+ ETL++F ++A + G MGGG+ Sbjct: 60 KFFSAGADL---KLFADGDKAMAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECA 116 Query: 119 SCCDIVVASDAAFFALSEVKLGLAPAAIS----PYVIAKMGVSQARRYFVSAERFPAARA 174 CDI +A + A AL E +GL P A P+++ G A+R + ER A A Sbjct: 117 LACDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLV---GEGWAKRMILCGERVNAETA 173 Query: 175 AAMGLVHEVASPDELDVRTAQLIDTLLANGPQAMRAAKQLVQVA--NPLQVTPELSEYTA 232 +GLV EV + R L P ++ A+KQL+Q A NPL + Sbjct: 174 LRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERE-- 231 Query: 233 GVIADLRASAEGREGLRAFLDKDAPAWTRQ 262 + DL + + REG++AFL+K +P W Q Sbjct: 232 -LFVDLFDTQDQREGVQAFLEKRSPQWKNQ 260 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 98.6 bits (244), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 81/265 (30%), Positives = 124/265 (46%), Gaps = 5/265 (1%) Query: 1 MSSAAHLDITPRGLATITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGKS 60 MSS + L G+A ITLNRP+ N+F + L D + + D +LLL G+ Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FSAGADLAWMRRMADASREDNLRDA--RQLALLMETLNSFPTPTLAKVQGAVMGGGVGLV 118 F AG DL R + +L ++ + L+++L + P P + V G G G L Sbjct: 61 FCAGQDLG-DRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLP 119 Query: 119 SCCDIVVASDAAFFALSEVKLGLAPAAISPYVIAKM-GVSQARRYFVSAERFPAARAAAM 177 CDIV+A+ +A F KLGL P Y + ++ G ++A + E+ A +A Sbjct: 120 LACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERW 179 Query: 178 GLVHEVASPDELDVRTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIAD 237 GL+ + D+L + A + A P AA + A+P PE + + Sbjct: 180 GLIWKCVDDDQL-MEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRA 238 Query: 238 LRASAEGREGLRAFLDKDAPAWTRQ 262 L S + REG+ AFL+K AP +T Q Sbjct: 239 LGRSRDYREGVTAFLEKRAPQFTGQ 263 >gi|300310158|ref|YP_003774250.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 281 Score = 97.1 bits (240), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 84/258 (32%), Positives = 130/258 (50%), Gaps = 3/258 (1%) Query: 3 SAAHLDITPRGLAT---ITLNRPEIHNAFDDQLIAHLIDLLTQARDDTRTCLLLLASTGK 59 S + GLA+ I +NRP N D +I + D ++LA+ G+ Sbjct: 9 SCVETETKKHGLASVGWIWINRPYDGNTIDRLTARDIIRAVGVFSKDPEVRAIVLAARGE 68 Query: 60 SFSAGADLAWMRRMADASREDNLRDARQLALLMETLNSFPTPTLAKVQGAVMGGGVGLVS 119 SFS G ++ W+R ADA L D R+LA L++ L PT+A+VQG G G GL + Sbjct: 69 SFSHGIEVQWLRAQADAPINGALDDTRKLAQLLQVLAECEKPTVARVQGKAAGIGAGLAA 128 Query: 120 CCDIVVASDAAFFALSEVKLGLAPAAISPYVIAKMGVSQARRYFVSAERFPAARAAAMGL 179 CDI +A+D A F + + +LG+APA I+P+++ +G Q +RYF + E AARA +GL Sbjct: 129 ACDICIAADHAHFTMGDDRLGMAPAVITPHLVRAIGARQCQRYFQTNELLTAARACELGL 188 Query: 180 VHEVASPDELDVRTAQLIDTLLANGPQAMRAAKQLVQVANPLQVTPELSEYTAGVIADLR 239 HE + LD + L+ +LL +A +L++ T ++S + +R Sbjct: 189 AHEAVPVELLDEKIGALLASLLLGDVHVQHSANKLIRAIAAAPPTVDMSVDAVRIGVRMR 248 Query: 240 ASAEGREGLRAFLDKDAP 257 A R L ++ AP Sbjct: 249 ACPSVRARLGLHAERSAP 266 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 811,495 Number of extensions: 31686 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 10 Length of query: 269 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 185 Effective length of database: 1,222,130 Effective search space: 226094050 Effective search space used: 226094050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)