BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0023.p (347 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [He... 637 0.0 gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-me... 72 5e-14 gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding prote... 58 1e-09 >gi|300312379|ref|YP_003776471.1| FAD-dependent monooxygenase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 637 bits (1642), Expect = 0.0, Method: Compositional matrix adjust. Identities = 318/322 (98%), Positives = 320/322 (99%) Query: 1 MKPLHVAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGL 60 M PLHVAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGL Sbjct: 1 MTPLHVAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGL 60 Query: 61 FSRIAALGQRIDRLYGADALSGKTVLDVRYDAIAGGRYGIAVHRAALFGVLLEAAQQEGI 120 FSRIAALGQRIDRLYGADALSGKTVLDVRYDAIAGGRYGIAVHRAALFGVLLEAAQQEGI Sbjct: 61 FSRIAALGQRIDRLYGADALSGKTVLDVRYDAIAGGRYGIAVHRAALFGVLLEAAQQEGI 120 Query: 121 EVETGQDISNLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHALHSCEPRPLPYG 180 EVETGQDI NLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHALHSCEPRPLPYG Sbjct: 121 EVETGQDIGNLEHTGAQACLTAADGRRIGGFDLVIDASGARSPLRRHALHSCEPRPLPYG 180 Query: 181 AFWVSLRAQPGLSFGHNSLVQRYRRASTMIGVLPIGRPTPQDEPMVAFFWSTRPDQVESL 240 AFWVSLRAQPGLSFGHNSLVQRYRRASTMIGVLPIGRPTPQDEPMVAFFWSTRPD+VESL Sbjct: 181 AFWVSLRAQPGLSFGHNSLVQRYRRASTMIGVLPIGRPTPQDEPMVAFFWSTRPDRVESL 240 Query: 241 KQAGLSAWKDGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPLPYGERLAIIGDAAHST 300 KQAGLSAWKDGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPLPYGERLAIIGDAAHST Sbjct: 241 KQAGLSAWKDGVHALWPECAPYLAQIDGFEQMTLARYGHHTLPLPYGERLAIIGDAAHST 300 Query: 301 SPQLGQGANMALLDARALSHAV 322 SPQLGQGANMALLDARALSHA+ Sbjct: 301 SPQLGQGANMALLDARALSHAL 322 >gi|300310340|ref|YP_003774432.1| FAD-dependent 2-polyprenyl-6-methoxyphenol hydroxylase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 72.4 bits (176), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 96/330 (29%), Positives = 141/330 (42%), Gaps = 39/330 (11%) Query: 6 VAIAGAGPAGLAMALYLKRAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGL---FS 62 + I G G +G++ A+ L+R G +V L+E Q G+G+ L P L L+ LG+ F Sbjct: 8 ILIIGGGFSGMSAAIDLRRRGAQVDLVELDAQWRNYGAGISLGPATLRALKQLGVLEAFL 67 Query: 63 RIAALGQ--RIDRLYGADALSGKTVLDVRYDAIAGGRYGIAVHRAALFGVLLEAAQQEGI 120 R A G R+ +G T D GG A+ R L +L +A + G Sbjct: 68 REGAAGDGVRLCLPHGPQVAELPTPRLASPDVPGGG----AILRPVLARILADATRAAGA 123 Query: 121 EVETGQDISNLEHTGAQACLTAADG--RRIGGFDLVIDASGARSPLRRHALHSCEPRPLP 178 +V G S + G + + DG RR +DLVI A G S LR H L P+P Sbjct: 124 DVRLGCTFSAVREVGHKVEVDFTDGQTRR---YDLVIGADGLYSKLRMH-LFPHAPKPRY 179 Query: 179 YG-AFWVSLRAQPGLSFGHNSLVQRYRRASTMIG--VLPIGRPTPQDEPMVAFFWSTRPD 235 G A W ++ +P Q + +G + P P +DE M F RP Sbjct: 180 SGQAVWRAVLPRP----------QEIVTCTMWMGPRIKPGVNPVSKDE-MYLFVTEPRPV 228 Query: 236 QVESLKQAGLSAWKDGVHALWPE-CAPYLA----QIDGFEQMTLARYGHHTLPLP-YGER 289 + + + + L E AP L Q+D ++ LP P Y R Sbjct: 229 N----EHVDPTTFVSHLRGLLEEFSAPVLKTIREQLDDNARIIFRPLEGLLLPRPWYQGR 284 Query: 290 LAIIGDAAHSTSPQLGQGANMALLDARALS 319 + +IGDA H+T+P L GA + + DA L+ Sbjct: 285 VVLIGDAVHATTPHLASGACIGIEDALVLA 314 >gi|300310665|ref|YP_003774757.1| monooxygenase FAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 384 Score = 57.8 bits (138), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 68/314 (21%), Positives = 123/314 (39%), Gaps = 38/314 (12%) Query: 24 RAGHRVSLLERFLQAAPVGSGLILQPTGLTVLQDLGLFSRIAALGQRIDRLYGADALSGK 83 + G V + E+ + +G+G+ + P + VL+ +G+ + G R D Y +G Sbjct: 25 QEGFNVRVYEQAPGFSRLGAGIHVGPNVMKVLRRIGIEDEMNRQGSRPDFWYSRHWQTGD 84 Query: 84 TVLDVRYDAIAGGRYG---IAVHRAALFGVLLEAAQQEGIEVETGQDISNLEHTGAQACL 140 + + A YG + VHR +L++A E + G+ ++ +E G L Sbjct: 85 VLAQIPLGDYAVKHYGASYLTVHRGDFHELLIKALPSERLSF--GKCLTKVEDRGDVVIL 142 Query: 141 TAADGRRIGGFDLVIDASGARSPLRRHALHSCEPRPLPYGAFWVSLRAQPGLSFGHNSLV 200 + ADG D+VI A G S +R L P+ Y H ++ Sbjct: 143 SFADGTTEEA-DIVIGADGVNSRIREELLGVEPPKYAGY--------------LAHRAVF 187 Query: 201 QRYRRASTMIGVLPIGRPTPQDEPMVAFFWSTRPDQVESLKQAGLSAW------------ 248 + M+ + D M+ +F +++ +++ + + W Sbjct: 188 PTPELKAGMLPFDACVKWWSDDRHMMVYFVTSKANELYYVTGVPVEKWDLNDRWLPSSKE 247 Query: 249 --KDGVHALWPECAPYLAQIDGFEQMT-LARYGHHTLPLPYGERLAIIGDAAHSTSPQLG 305 ++ H P A +D ++T + LPL RL ++GDA H P + Sbjct: 248 EMRETFHGWHPTVQ---ALVDATVEVTKWSLLERDPLPLWSRGRLVLLGDACHPMKPHMA 304 Query: 306 QGANMALLDARALS 319 QGA MA+ D L+ Sbjct: 305 QGAAMAIEDGAMLA 318 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,215,599 Number of extensions: 57236 Number of successful extensions: 194 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 192 Number of HSP's successfully gapped: 3 Length of query: 347 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 261 Effective length of database: 1,212,660 Effective search space: 316504260 Effective search space used: 316504260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)