BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0109.p (696 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (... 1410 0.0 gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbas... 81 2e-16 >gi|300311919|ref|YP_003776011.1| non-hemolytic phospholipase C (phosphatidylcholine choline phospho hydrolase) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 1410 bits (3650), Expect = 0.0, Method: Compositional matrix adjust. Identities = 688/696 (98%), Positives = 688/696 (98%) Query: 1 MKRKSHDRRHFLKSAARPGSAALALTALPASIRKALAVAPASPTGTIKDVKHVVILMQEN 60 MKRKS DRRHFLKSAAR GSAALALTALPASIRKALAVAPASPTGTIKDVKHVVILMQEN Sbjct: 1 MKRKSQDRRHFLKSAARTGSAALALTALPASIRKALAVAPASPTGTIKDVKHVVILMQEN 60 Query: 61 RSFDHYFGCLRGVRGFADPIPRPLPDGSPVFHQRDGQGRLVLPYRMEMATTSAGRAGGNL 120 RSFDHYFGCLRGVRGFADPIPRPLPDGSPVFHQRDGQGRLVLPYRMEMATTSAGRAGGNL Sbjct: 61 RSFDHYFGCLRGVRGFADPIPRPLPDGSPVFHQRDGQGRLVLPYRMEMATTSAGRAGGNL 120 Query: 121 PHAWKDQHAAWNRGDYDKWIEAKKDTVTMAYFTREDLPFHYALADAFTLCDAYHCSVLGS 180 PHAWKDQHAAWNRGDYDKWIEAKKDTVTMAYFTREDLPFHYALADAFTLCDAYHCSVLGS Sbjct: 121 PHAWKDQHAAWNRGDYDKWIEAKKDTVTMAYFTREDLPFHYALADAFTLCDAYHCSVLGS 180 Query: 181 TNPNRFHLMSGGIDLQGRAGGPMTFQPRTGSWSGVPGERRSGADSFGWTTYPERLEQHGV 240 TNPNRFHLMSGGIDLQGRAGGPMTFQPRTGS SGVPGERRSGADSFGWTTYPERLEQHGV Sbjct: 181 TNPNRFHLMSGGIDLQGRAGGPMTFQPRTGSCSGVPGERRSGADSFGWTTYPERLEQHGV 240 Query: 241 SWKVYQGTEELHKDHGDYPSDFNVLQYFFAQYQNAAPDSPLWQKACSKFTLADLARDVNS 300 SWKVYQGTEELHKDHGDYPSDFNVLQYFFAQYQNAAPDSPLWQKACSKFTLADLARDVNS Sbjct: 241 SWKVYQGTEELHKDHGDYPSDFNVLQYFFAQYQNAAPDSPLWQKACSKFTLADLARDVNS 300 Query: 301 GSLPQVSWLMPPKLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDGFF 360 G LPQVSWLMPPKLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDGFF Sbjct: 301 GKLPQVSWLMPPKLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNYDENDGFF 360 Query: 361 DHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISPWSKGGYV 420 DHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISPWSKGGYV Sbjct: 361 DHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISPWSKGGYV 420 Query: 421 CSEVFDHTSVIRFLEQVFDVHEPNISPWRRAVCGDLTSAFDFSRRDAASPVLPDTSAYRA 480 CSEVFDHTSVIRFLEQVFDVHEPNISPWRRAVCGDLTSAFDFSRRDAASPVLPDTSAYRA Sbjct: 421 CSEVFDHTSVIRFLEQVFDVHEPNISPWRRAVCGDLTSAFDFSRRDAASPVLPDTSAYRA 480 Query: 481 LSDAQARLPAPVAPSSPAMPQQERGMRPARALPYVLAVDDRVVLDQAALQLDFLNPGKAG 540 LSDAQARLPAPVAPSSPAMPQQERGMRPARALPYVLAVDDRVV DQ ALQLDFLNPGKAG Sbjct: 481 LSDAQARLPAPVAPSSPAMPQQERGMRPARALPYVLAVDDRVVPDQDALQLDFLNPGKAG 540 Query: 541 VVYQVYARQESERYQTFTVQPGKSLTAQWRGVGPVSGYDLKVYGPNGFLRELRGSLERGG 600 VVYQVYARQESERYQTFTVQPGKSLTAQWRGVGPV GYDLKVYGPNGFLRELRGSLERGG Sbjct: 541 VVYQVYARQESERYQTFTVQPGKSLTAQWRGVGPVGGYDLKVYGPNGFLRELRGSLERGG 600 Query: 601 GVEPVVQARHDGERGELVLALLNPGRQALRLVLRDQAHGQATRTVTLAPGQSATERWPLA 660 GVEPVVQARHDGERGELVLALLNPGRQAL LVLRDQAHGQATRTVTLAPGQSATERWPLA Sbjct: 601 GVEPVVQARHDGERGELVLALLNPGRQALHLVLRDQAHGQATRTVTLAPGQSATERWPLA 660 Query: 661 SSHHWYDLLVQAEDRPAFARRLAGHVETGRPSFFVS 696 SSHHWYDLLVQAEDRPAFARRLAGHVETGRPSFFVS Sbjct: 661 SSHHWYDLLVQAEDRPAFARRLAGHVETGRPSFFVS 696 >gi|300310916|ref|YP_003775008.1| phospholipase C protein [Herbaspirillum seropedicae SmR1] Length = 558 Score = 81.3 bits (199), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 118/509 (23%), Positives = 184/509 (36%), Gaps = 155/509 (30%) Query: 48 KDVKHVVILMQENRSFDHYFGCLRGVRGFADPIPRPLPDGSPV-FHQRDGQGRLV--LP- 103 + VK +V++ ENRSF++ F G+ +PL P + QRD G L+ LP Sbjct: 61 EQVKTIVVIYAENRSFNNLFADFPGLE-------KPLSALKPSDYQQRDRDGSLLKQLPP 113 Query: 104 ---------YRMEMATTSAGRA-GGNLP------------------------HAWKDQHA 129 +E T AG+ NLP H + Sbjct: 114 AWSGVVNQEQTVEGVTYEAGKQYQTNLPNAPYALKGPQGEALPLGLVTRDLWHVFYQNQM 173 Query: 130 AWNRGDYDKWIE-AKKDTVTMAYFTREDLPFHYALADA---FTLCDAYHCSVLGST---- 181 N G D ++ A +TM ++ D +H L D F LCD + G + Sbjct: 174 QINGGKNDGFVAWADSGGLTMGHYA--DTRYHLRLWDVAREFVLCDNFFQGAFGGSFLNH 231 Query: 182 ----------NPNRFHLMSGGIDLQGRAGGPMTFQPRTGSWSGVPGERRSGADSFG---- 227 PN + +S + + P++ P G P G FG Sbjct: 232 QYLICATAPYYPNVMNSVSRTQVAKTKNNDPLS--PELVPAEGSPTSALQGPPKFGPSAL 289 Query: 228 --------------WTTYP--------------------------ERLEQHGVSWKVY-- 245 W T+ ++L + G+ W Y Sbjct: 290 TPDGYAVNTMAPPYWPTWSRDRNNPAYAEPNQPSVLVPQTHEHIGDKLNKKGIDWAWYAG 349 Query: 246 --QGTEELHKDHGDYPS--DFNVLQYFFAQYQNAAPDSPLWQ-KACSKFTLADLAR---- 296 Q T + KD P +F F ++N P++P + K L D AR Sbjct: 350 AWQATLDEFKDSKGIPKIPNFQYHHQPFNYFKNLGPENPAERNKRLRDGGLGDEARTNRF 409 Query: 297 --DVNSGSLPQVSWLMPP-KLSCEHPDRTPGYGAHYISQVLDILTANPALWSSTVLLVNY 353 DV +G LP V++ P L+ G +I+ ++ L A+P W + V++V + Sbjct: 410 LADVEAGRLPPVTFYKPQGNLNLHAGYADIASGDRHIAHIIKSLQASPQ-WKNMVVVVTF 468 Query: 354 DENDGFFDHVVPPTPPLHAGQGLSTVETSAEFHRKGDYVNPADDLPAGLGARVPMMVISP 413 DEN G++DHV PP KGD P G RVP +V+SP Sbjct: 469 DENGGWWDHVAPP---------------------KGDRWGP--------GTRVPALVVSP 499 Query: 414 WSKGGYVCSEVFDHTSVIRFLEQVFDVHE 442 +++ G V V+D S++R + + FD+ + Sbjct: 500 FARKGTVDHTVYDTASILRLITRTFDLEK 528 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,403,908 Number of extensions: 108292 Number of successful extensions: 284 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 281 Number of HSP's successfully gapped: 2 Length of query: 696 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 604 Effective length of database: 1,184,250 Effective search space: 715287000 Effective search space used: 715287000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)