BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0089.p (832 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311937|ref|YP_003776029.1| poly(3-hydroxyalkanoate) synthe... 1687 0.0 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 74 3e-14 >gi|300311937|ref|YP_003776029.1| poly(3-hydroxyalkanoate) synthetase [Herbaspirillum seropedicae SmR1] Length = 832 Score = 1687 bits (4368), Expect = 0.0, Method: Compositional matrix adjust. Identities = 823/832 (98%), Positives = 825/832 (99%) Query: 1 MINRKKPVSAAPNDGHAHPDDHQPLAASASWGDVPAYLQDCLQRSFLFLDTLLDRGNDYL 60 MINRKKPVSAAPNDGHAHPDD QP+AASASWGDVPAYLQDCLQRSFLFLDTLLDRGNDYL Sbjct: 1 MINRKKPVSAAPNDGHAHPDDDQPVAASASWGDVPAYLQDCLQRSFLFLDTLLDRGNDYL 60 Query: 61 EHLEQGTPPLLKFAHEVVLDGHDLPQPCNYALLRLQPPAGLPVNPQARPVVVVDPRAGHG 120 EHLEQGTPPLLKFAHEVVLDGHDLPQ CNYALLRLQPPAGLPVNPQARPVVVVDPRAGHG Sbjct: 61 EHLEQGTPPLLKFAHEVVLDGHDLPQACNYALLRLQPPAGLPVNPQARPVVVVDPRAGHG 120 Query: 121 PGIGGFKFDSEVGMAMKAGHTVYFVTFRPEPEDGQTLVTVMDAEARFLEEVIRRHPQCSA 180 PGIGGFKFDSEVGMAMKAGHTVYFVTFRPEPEDGQTLVTVMDAEARFLEEVIRRHPQCSA Sbjct: 121 PGIGGFKFDSEVGMAMKAGHTVYFVTFRPEPEDGQTLVTVMDAEARFLEEVIRRHPQCSA 180 Query: 181 KPVVIGNCQAGWAMMTLDAVRPELFGPLMMVGAPASYWAGSSTLNPMRYSGANLGGTWLA 240 KPVVIGNCQAGWAMMTLDAVRPELFGPLMMVGAPASYWAGSSTLNPMRYSGANLGGTWLA Sbjct: 181 KPVVIGNCQAGWAMMTLDAVRPELFGPLMMVGAPASYWAGSSTLNPMRYSGANLGGTWLA 240 Query: 241 SLAADLGADRFDGAYLVENFEKLNPANTFWNKYYNLWSGVDQEAARFLEFERWWGGFFRM 300 SLAADLGADRFDGAYLVENFEKLNPANTFWNKYYNLW+GVDQEAARFLEFERWWGGFFRM Sbjct: 241 SLAADLGADRFDGAYLVENFEKLNPANTFWNKYYNLWAGVDQEAARFLEFERWWGGFFRM 300 Query: 301 TGAEIEAITENLFVGNKLARGELQVRGKPVNLREISAPIVVFASWGDNITPPPQALNWII 360 TGAEIEAITENLFVGNKLARGELQVRGKPVNLREISAPIVVFASWGDNITPPPQALNWII Sbjct: 301 TGAEIEAITENLFVGNKLARGELQVRGKPVNLREISAPIVVFASWGDNITPPPQALNWII 360 Query: 361 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGSVALKEHDQLVSSLDVIESLPPGLYEM 420 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGSVALKEHDQLVSSLDVIESLPPGLYEM Sbjct: 361 DTWGDERAIAAAGRTIIYVLHESVGHLGIFVGGSVALKEHDQLVSSLDVIESLPPGLYEM 420 Query: 421 KLERKDGRPTQRWDALEPGDYTVHYEHRTMEDLRKLNPEGREEEAIFSTISQWSQMNAQF 480 KLERKDGRPTQRWD LEPGDYTVHYEHRTMEDLRKLNPEGREEEAIFSTISQWSQMNAQF Sbjct: 421 KLERKDGRPTQRWDVLEPGDYTVHYEHRTMEDLRKLNPEGREEEAIFSTISQWSQMNAQF 480 Query: 481 YKTWVRPWVRMTATRESANALMRMNPLRMQRQLFSDAHPAAAFIRQQAAQARAQRVQLDP 540 YKTWVRPWVRMTATRESANALMRMNPLRMQRQLFSDAHPAAAFIRQQAAQARAQRVQLDP Sbjct: 481 YKTWVRPWVRMTATRESANALMRMNPLRMQRQLFSDAHPAAAFIRQQAAQARAQRVQLDP 540 Query: 541 QHPLKQYEARMAQKITDELNAWRDQRDARTVRMTRQMFGPNGLGAWLPPREPDAELAQRW 600 QHPLKQYEARMAQKITDELNAWRDQRDARTVRMTRQMFGPNGLGAWLPPREPDAELAQRW Sbjct: 541 QHPLKQYEARMAQKITDELNAWRDQRDARTVRMTRQMFGPNGLGAWLPPREPDAELAQRW 600 Query: 601 AQQELGRYRETVIGQIAEGGFAEAVCRIVIAGMISIGVFERRSLRLARLLAQLPNMHASV 660 AQQELGRYRETVIGQIAEGGFAEAVCRIVIAGMISIGVFERRSLRLARLLAQLPNMHASV Sbjct: 601 AQQELGRYRETVIGQIAEGGFAEAVCRIVIAGMISIGVFERRSLRLARLLAQLPNMHASV 660 Query: 661 SAQTNWVQLLKEQARITAVAPVEALNALGQMLPDAASRERALALSAAVMMIEPTLANPRS 720 SAQTNWVQLLKEQARITAVAPVEALNALGQMLPDAASRERALALSAAVMMIEPTLANPRS Sbjct: 661 SAQTNWVQLLKEQARITAVAPVEALNALGQMLPDAASRERALALSAAVMMIEPTLANPRS 720 Query: 721 EIIELLIGTLDVNPDKVIALARKLTSAIGAGSDEVTVPAGELEAAPVKRPRASSPRGPVA 780 EIIELLIGTLDVNPDKVIALARKLTSAIGAGSDEVTVPAGELEAAPVKRPRASSPR A Sbjct: 721 EIIELLIGTLDVNPDKVIALARKLTSAIGAGSDEVTVPAGELEAAPVKRPRASSPRRTEA 780 Query: 781 KPAPTPARTQRKAAAPAQPSKAAKSVTSAKSLKPAKPSSAATTKRSARSRSN 832 KPAPTPARTQRKAAAPAQPSKAAKSVTSAKSLKPAKPSSA TTKRSARSRSN Sbjct: 781 KPAPTPARTQRKAAAPAQPSKAAKSVTSAKSLKPAKPSSATTTKRSARSRSN 832 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 74.3 bits (181), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 71/299 (23%), Positives = 125/299 (41%), Gaps = 43/299 (14%) Query: 109 PVVVVDPRAGHGPGIGGF-KFDSEVGMAMKAG-HTVYFVTFRPEPEDGQTLVTVMDAEAR 166 P ++V P AGH I + + S V + G V + + D + L Sbjct: 87 PTLIVAPYAGHTSVIADYDQGQSLVETLLGNGVDHVLLIDWHSASADMKDLEI-----DN 141 Query: 167 FLEEVIRRHPQCSAKPVVIGNCQAGWAMMTLDAVRPELFGPLMMVGAPASYWAGSSTLN- 225 +L ++ + + ++G CQ GW + A PE L++ G+P AG L Sbjct: 142 YLADLCVVIDELGGRVRLVGLCQGGWISAMIAARFPEKVARLVLAGSPIDAGAGEGPLKQ 201 Query: 226 -----PMRYSGANLGGTWLASLAADL---GADRFDGAYLVENFEKLNPANTFWNKYYNLW 277 P+R+ DL G G+++++ ++ ++P ++N++ L Sbjct: 202 MVQRLPLRFY-------------EDLVRSGGGLMRGSFMLQGWKNMHPDEHYFNEHLELL 248 Query: 278 SGVDQEA--ARFLEFERWWGGFFRMTGAEIEAITENLFVGNKLARGELQVRGKPVNLREI 335 +D A A+ F W+ + G ++F N LARG+ G+ ++LR+I Sbjct: 249 QHIDDPAYLAKRETFAAWYETTVDLPGRWYLQAIGHIFKDNLLARGKYVALGQQLDLRQI 308 Query: 336 SAPIVVFASWGDNITPPPQALNWIIDTWGDERAIAAAGRTIIYVLHESV--GHLGIFVG 392 + P+ + A D+IT P Q LN A A G + + V GH+G+F+G Sbjct: 309 TCPLYLLAGERDDITTPEQVLN----------AAALVGTPAAQITQKIVPGGHIGLFMG 357 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,661,715 Number of extensions: 121221 Number of successful extensions: 507 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 508 Number of HSP's successfully gapped: 2 Length of query: 832 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 739 Effective length of database: 1,179,515 Effective search space: 871661585 Effective search space used: 871661585 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)