BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0009.p (495 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 974 0.0 gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 283 3e-77 gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 220 3e-58 gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 148 8e-37 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 148 1e-36 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 974 bits (2519), Expect = 0.0, Method: Compositional matrix adjust. Identities = 493/495 (99%), Positives = 494/495 (99%) Query: 1 MNRINSSAFRLRAIAAVAALAGVLGLSGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGA 60 MNRINSSAFRLRAIAAVAALAGVLGLSGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGA Sbjct: 1 MNRINSSAFRLRAIAAVAALAGVLGLSGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGA 60 Query: 61 PNGQWPGSDWVRQFGDAQLVALVEQALTSSPSLQQARARISAASALAEWRGAPLLPSVNA 120 PNGQWPGSDWVRQFGDAQLVALVEQALTSSPSLQQARARI+AASALAE RGAPLLPSVNA Sbjct: 61 PNGQWPGSDWVRQFGDAQLVALVEQALTSSPSLQQARARIAAASALAESRGAPLLPSVNA 120 Query: 121 EASVTRNQFSSTTIYPPPYGGNWYNEKKAGLNVGYELDLWNKNQAALAQAISSEKAAQAS 180 EASVTRNQFSSTTIYPPPYGGNWYNEKKAGLNVGYELDLWNKNQAALAQAISSEKAAQAS Sbjct: 121 EASVTRNQFSSTTIYPPPYGGNWYNEKKAGLNVGYELDLWNKNQAALAQAISSEKAAQAS 180 Query: 181 EQEARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQIERDQS 240 EQEARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQIERDQS Sbjct: 181 EQEARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQIERDQS 240 Query: 241 RTSSADARAQLAQSEGQIVLLRQQLGALAGKGPDYGLQLAPPALQGLATPGLPAELPLNL 300 RTSSADARAQLAQSEGQIVLLRQQLGALAGKGPDYGLQLAPPALQGLATPGLPAELPLNL Sbjct: 241 RTSSADARAQLAQSEGQIVLLRQQLGALAGKGPDYGLQLAPPALQGLATPGLPAELPLNL 300 Query: 301 MGRRPDIVAARWQVEAASRGVDVAKARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFGP 360 MGRRPDIVAARWQVEAASRGVDVAKARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFGP Sbjct: 301 MGRRPDIVAARWQVEAASRGVDVAKARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFGP 360 Query: 361 AITLPIFEGGALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIRSE 420 AITLPIFEGGALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIRSE Sbjct: 361 AITLPIFEGGALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIRSE 420 Query: 421 ALQAAERAYALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGG 480 ALQAAERAYALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGG Sbjct: 421 ALQAAERAYALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGG 480 Query: 481 GFDAQRDGLAYGAQP 495 GFDAQRDGLAYGAQP Sbjct: 481 GFDAQRDGLAYGAQP 495 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 283 bits (723), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 186/478 (38%), Positives = 272/478 (56%), Gaps = 6/478 (1%) Query: 12 RAIAAVAALAGVLGLSGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGAPNGQWPGSDWV 71 RA++ +AA+ ++GCASF+ +G Q + +Q+SL+ A WP W Sbjct: 24 RAVSVLAAVLAATLMAGCASFSDLGERAQPKSIDRYQSQQSLA---ASAVQAAWPSDQWW 80 Query: 72 RQFGDAQLVALVEQALTSSPSLQQARARISAASALAEWRGAPLLPSVNAEASVTRNQFSS 131 R +GDAQL AL+++AL S+PS+ A+AR+ A A+ +GA L P V+A AS+ R + S Sbjct: 81 RVYGDAQLNALIDEALQSAPSMAVAKARLMKAEGAAQQQGAALYPQVSANASLDRMKQSY 140 Query: 132 TTIYPPPYGGNWYNE-KKAGLNVGYELDLWNKNQAALAQAISSEKAAQASEQEARLALTA 190 PP + YN +A L+ YE+D W KN+AALA A S +A++A +AR+ L Sbjct: 141 NNGVPPDFVPKDYNNATRATLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLAT 200 Query: 191 SIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQIERDQSRTSSADARAQ 250 SI + Y++LA YA D ++ + R + +R GL+++ Q A A+A Sbjct: 201 SIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQRFTNGLETRGSVKQMEARRAIAQAD 260 Query: 251 LAQSEGQIVLLRQQLGALAGKGPDYGLQLAPPALQGLATP-GLPAELPLNLMGRRPDIVA 309 L ++ I L R +L AL G GPD GLQL P + L+ P LPA+LP+ L+GRRPDIVA Sbjct: 261 LKATDESIGLQRNKLAALLGAGPDRGLQLTRPQID-LSRPFALPAQLPVELLGRRPDIVA 319 Query: 310 ARWQVEAASRGVDVAKARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFGPAITLPIFEG 369 AR + EAA + + VA+A FYP++NL+A GF +L A S + GPAI+LPIF G Sbjct: 320 ARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGIDMLTRAGSDIGSIGPAISLPIFNG 379 Query: 370 GALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIRSEALQAAERAY 429 G LR + ASY+ AVA Y++ + A DVA + A +L A AAE AY Sbjct: 380 GRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGRLADVQAAADAAEEAY 439 Query: 430 ALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGGGFDAQRD 487 + RY GL + L +L+AQ +++ + + L+++ VAL +ALGGG+ A D Sbjct: 440 RIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSRMFTLDVALVRALGGGYRAADD 497 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 220 bits (560), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 175/485 (36%), Positives = 252/485 (51%), Gaps = 10/485 (2%) Query: 1 MNRINSSAFRLRAIAAVAALAGVLGLSGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGA 60 M RI AF + V AL+ L ++GCA GI + AQ D A + A Sbjct: 1 MTRI---AFSKLPVLTVIALS--LTMAGCADQGGI---QPQAQVRDVAVLDAGQAIRQAA 52 Query: 61 PNGQWPGSDWVRQFGDAQLVALVEQALTSSPSLQQARARISAASALAEWRGAPLLPSVNA 120 WP W + +GDAQL LV +A+ +PS+ A AR+ A ++A A LPS Sbjct: 53 SEAGWPQRAWWQAYGDAQLDQLVTRAVAGNPSMAVAAARVRQAQSMAVVAHAGELPSAQL 112 Query: 121 EASVTRNQFSSTTIYPPPYGGNWYNEKKAGLNVGYELDLWNKNQAALAQAISSEKAAQAS 180 + SV R +S Y + A L + Y +DLW++N+ A +A A A Sbjct: 113 DVSVGRKDWSDNVYYGAAFRNKLTWNNTATLGLSYNVDLWDRNKNATLRAQDEMHAVAAD 172 Query: 181 EQEARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQIERDQS 240 + A+L L ++V Y QLA QY L D ++ + + + + R + GL +Q+E Q+ Sbjct: 173 ARAAQLDLEGNVVRTYVQLALQYGLLDNTEALLKEEQKILDLAHRRFKGGLGTQLEITQA 232 Query: 241 RTSSADARAQLAQSEGQIVLLRQQLGALAGKGPDYGLQLAPPALQGLATPGLPAELPLNL 300 + + R Q+ I L+R QL AL G GP + P +Q + GLP+ LP +L Sbjct: 233 ESPLPETRRQIEALHENIALIRNQLAALLGAGPGAADGVTRPGMQLQTSLGLPSALPADL 292 Query: 301 MGRRPDIVAARWQVEAASRGVDVAKARFYPDINLSAMIGFDTLLDS--NPFTAASKSIAF 358 +GRRPDI AARW+VEAA++G+ VAKA FYP+INL A IG + +A + + Sbjct: 293 VGRRPDISAARWRVEAAAKGISVAKAAFYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTY 352 Query: 359 GPAITLPIFEGGALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIR 418 GPAI+LPIFEGG LR L A Y++ V YN L+ A ++ Q+ + + RQ Sbjct: 353 GPAISLPIFEGGRLRGQLGAASAGYDIEVEHYNALLSQALKGISDQVVTLQSVARQQEQA 412 Query: 419 SEALQAAERAYALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKAL 478 +++QAA++ +A Y+ GL L +L AQT L Q+Q + LQAQR AL AL Sbjct: 413 QQSVQAAQKNVDIATRAYQRGLTDYLNVLHAQTQWLRQQQIVQQLQAQRLAAYAALQTAL 472 Query: 479 GGGFD 483 GGG + Sbjct: 473 GGGIE 477 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 148 bits (374), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 140/452 (30%), Positives = 224/452 (49%), Gaps = 26/452 (5%) Query: 43 QAGDFATQRSLSDPNPGAPNGQWPGSDWVRQFGDAQLVALVEQALTSSPSLQQARARISA 102 Q G+ +Q + P G+W W + FGD +L AL + A ++ L+ A AR+ Sbjct: 56 QTGENGSQWKTAQPAEDIARGEW----W-KIFGDEKLNALEDTAQQANQDLKAAAARLGQ 110 Query: 103 ASALAEWRGAPLLPSVNAEASVTRNQFSSTTIYPPPYGGNWYNE-KKAGLNVGYELDLWN 161 A AL A P V+A TR + S + P N +A +NV YE+DL+ Sbjct: 111 ARALTRDARAGYFPQVDAGVGATRQRPSPASQGLPADADTRPNTLYRAQVNVSYEVDLFG 170 Query: 162 KNQAALAQAISSEKAAQASEQEARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEK 221 + + + A + + ++A LAL A + Y + A + + TVD R + Sbjct: 171 RVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAAALYRGTVDLRQESLR 230 Query: 222 ITAERLRTGLDSQIERDQSRTSSADARAQLAQSEGQIVLLRQQ-------LGALAGKGPD 274 + R G D S A ARA+LA ++ + + + +Q L L G+ P Sbjct: 231 LVQRRFDEG-------DISEVDVARARAELASAQSEALGIARQRATAEHALAILLGRTP- 282 Query: 275 YGLQLAPPALQGLATPGLPAELPLNLMGRRPDIVAARWQVEAASRGVDVAKARFYPDINL 334 L P L LA +P LP L+ RRPDI AA + AA+ V VAK+ F+P +NL Sbjct: 283 AEFSLPPQPLTRLAVT-VPPGLPSALLERRPDIAAAERAMAAANARVGVAKSAFFPSLNL 341 Query: 335 SAMIGFDTLLDSNPFTAASKSIAFGP----AITLPIFEGGALRAGLKGEYASYELAVATY 390 + +G+++ + F +S++ GP A+++P+F+GG +AG+ ASYE VA+Y Sbjct: 342 TGGLGYESAQLGDLFNWSSRTFLLGPLVGTALSMPLFDGGRRQAGVDRARASYEEQVASY 401 Query: 391 NKTLNDAYADVARQIAAIHATERQLPIRSEALQAAERAYALARERYRLGLVSQLTLLSAQ 450 T+ +A+ +V +A + ++Q + EA+ A+ RA L+ +YR G VS +++ A Sbjct: 402 RGTVLNAFREVEDNLANLRILDQQNRAQDEAVAASARAAKLSHTQYREGAVSYFSVIDAD 461 Query: 451 TGVLAQRQAMVALQAQRRDQQVALYKALGGGF 482 VL Q++A V L +R V L +ALGGG+ Sbjct: 462 RTVLQQQRASVTLDGERARSTVNLIRALGGGW 493 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 148 bits (373), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 127/436 (29%), Positives = 209/436 (47%), Gaps = 21/436 (4%) Query: 70 WVRQFGDAQLVALVEQALTSSPSLQQARARISAASALAEWRGAPLLPSVNA----EASVT 125 W F D +L ++ AL ++ L+ A I A A A PS++A AS T Sbjct: 73 WREFFLDPRLQQVIAAALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRT 132 Query: 126 RNQFSSTTIYPPPYGGNWYNEKKAGLNVG---YELDLWNKNQAALAQAISSEKAAQASEQ 182 + S+T G+ + + VG YELD + + + A+ S + + + Sbjct: 133 PAELSTT--------GSAAVSHQQSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRR 184 Query: 183 EARLALTASIVTVYSQLAAQYALHDILQSTVDQRTSLEKITAERLRTGLDSQIERDQSRT 242 ++L A + T + LA+ L + Q T ++ ++ G S ++ Sbjct: 185 STHISLVAEVATAWLTLASDRDLLKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEA 244 Query: 243 SSADARAQLAQSEGQIVLLRQQLGAL-AGKGPDYGLQLAPPALQGLAT--PGLPAELPLN 299 + ARA +A+ Q+ Q L AL G D L P L A+ P PA LP Sbjct: 245 TVESARADVARYASQV---EQDLNALRLLVGADIDAALLPAGLPDTASVLPATPAGLPSQ 301 Query: 300 LMGRRPDIVAARWQVEAASRGVDVAKARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFG 359 ++ RRPD++AA +++A+ + A+A F+P I+L+A G + F A S S F Sbjct: 302 VLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFS 361 Query: 360 PAITLPIFEGGALRAGLKGEYASYELAVATYNKTLNDAYADVARQIAAIHATERQLPIRS 419 P+I LPIF G+LRA L + ++ VA Y K + A+ +VA +A +++L + Sbjct: 362 PSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQG 421 Query: 420 EALQAAERAYALARERYRLGLVSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALG 479 +A A+E+A+ L+ RYR G+ S LT L AQ + + +Q +++L+ + ++ LYK LG Sbjct: 422 KATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLG 481 Query: 480 GGFDAQRDGLAYGAQP 495 GG+ Q + G QP Sbjct: 482 GGWSEQTQPMQPGGQP 497 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.129 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,255,244 Number of extensions: 44835 Number of successful extensions: 274 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 18 Length of query: 495 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 406 Effective length of database: 1,198,455 Effective search space: 486572730 Effective search space used: 486572730 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)