BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0008.p (406 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 810 0.0 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 281 5e-77 gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 114 2e-26 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 77 3e-15 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 810 bits (2092), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/406 (99%), Positives = 406/406 (100%) Query: 1 MSDSTTQQQPQANNNGNGKRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVGGN 60 MSDSTTQQQPQANNNGNGKRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVGGN Sbjct: 1 MSDSTTQQQPQANNNGNGKRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVGGN 60 Query: 61 VVQISAQVGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFL 120 VVQISAQVGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFL Sbjct: 61 VVQISAQVGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFL 120 Query: 121 NNDTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVAHARDAVKSAVAALDQARAA 180 NNDTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDV+HARDAVKSAVAALDQARAA Sbjct: 121 NNDTLAANVAAADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQARAA 180 Query: 181 AAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAG 240 AAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAG Sbjct: 181 AAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRSVQVGQRIAAG 240 Query: 241 NPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAF 300 NPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAF Sbjct: 241 NPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGGAF 300 Query: 301 SLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRALEAVPTN 360 SLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRALEAVPTN Sbjct: 301 SLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGDGRALEAVPTN 360 Query: 361 YQTNVYDDLGKQADAIVERIISDNASGLPQAHKSKAAAAPVAVPHT 406 YQTNVYDDLGKQADAIV+RIISDNASGLPQAHKSKAAAAPVAVPHT Sbjct: 361 YQTNVYDDLGKQADAIVDRIISDNASGLPQAHKSKAAAAPVAVPHT 406 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 281 bits (720), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 156/359 (43%), Positives = 218/359 (60%), Gaps = 28/359 (7%) Query: 19 KRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVKAD 78 KRK+ + V+ + A+ YW+L + ETD+AY + ++ + G V AV Sbjct: 31 KRKKLFSIFGGVVAIAAIGYGAYWYLIGSRYVETDNAYTATEIATVTPAINGIVAAVDVV 90 Query: 79 DTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFLNNDTLAANVAA------- 131 DTQ VK G LV +D AD RLA+DQA A L + R+ + F N+ LAA V A Sbjct: 91 DTQAVKKGDVLVRIDDADARLAVDQAAADLDRTERKVKGFFANDAGLAAQVLAREAEQKR 150 Query: 132 -------ADSNLARAREDLQRRQAGLSSGAVSQEDVAHARDAVKSAVAALDQARAAAAAN 184 A ++L RA DLQRR+A SG+VS E++++AR A+ +A A L A AA + Sbjct: 151 ASAQLLSAQADLKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQS 210 Query: 185 RA--------------LTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRS 230 RA LT +T+V ++P V+ A + A L+ R + APV G +++R Sbjct: 211 RANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLEQAKLDLERTVLRAPVDGVIARRQ 270 Query: 231 VQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVI 290 VQVGQR+ +G L+++VPL+Q+ +DANFKE QL +RIGQPV + AD+YG SV+Y G V Sbjct: 271 VQVGQRVQSGATLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKADLYGGSVEYHGVVT 330 Query: 291 GFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHG 349 G S GTG AF+++PAQNATGNWIKVVQR+PVRI+LDP+++ PL +GLS +D G Sbjct: 331 GLSGGTGSAFAVIPAQNATGNWIKVVQRLPVRISLDPKELAQRPLSVGLSMVVEIDTRG 389 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 63/140 (45%), Positives = 86/140 (61%), Gaps = 6/140 (4%) Query: 209 AYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRI 268 A +N I A V G + + V+VGQ ++ G +M +VP + ++ ANFKE+Q+ +RI Sbjct: 236 ASVNLGYTEIRASVDGRIGDKQVRVGQFVSPGTRMMTVVPT-RFYVSANFKETQIGIMRI 294 Query: 269 GQPVEVIADVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALD-- 326 GQP + D V++ G V S GTG FSLLP QNATGN+ K+VQRVPVR+A+D Sbjct: 295 GQPAIIKVDAL-PGVEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRVPVRVAIDAT 353 Query: 327 PEQVRAHPLRIGLSTTATVD 346 PE+ RA GLS T V+ Sbjct: 354 PEETRA--FVAGLSVTVEVN 371 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 76.6 bits (187), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 100/341 (29%), Positives = 155/341 (45%), Gaps = 41/341 (12%) Query: 22 RQLILLTLVLLVIAVACFLY-WFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVKADDT 80 R LI + LL I +A L+ ++H+ + T D V VV I+ V G VVA+ D Sbjct: 8 RVLITTAIFLLAILLAWSLWQHYMHSPW---TRDGRVRAEVVNIAPDVSGQVVALPVRDN 64 Query: 81 QVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFLNNDTLAANVAAADSNLARAR 140 Q+VK G L+ +D A LAL QA+AA V A + L R Sbjct: 65 QLVKKGDLLMEIDPARYTLALQQAEAA---------------------VTARKAELDVRR 103 Query: 141 EDLQRRQAGLSSGAVSQEDVAHARDAVKSAVAALDQ-ARAAAAANRALTDHTSVTEHPNV 199 +++ R+A L+ A V S A D ++ A+ + + Sbjct: 104 AEVEARRADLNMRRAQARRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQLQGAQAQ 163 Query: 200 LQAATAVRN-AYLNYARVNIVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANF 258 QAA + R+ A LN R + +PV G+++ +V+ G AAG +A++ W+ A F Sbjct: 164 YQAALSQRDTARLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYAYF 223 Query: 259 KESQLQHIRIGQPVEVIADVYGSSVKYKGTVIGFSAGTGG-----AFSLLPAQNATGNWI 313 +E++L + IG I + G+S+ +G V + G + L N T NW+ Sbjct: 224 EETKLPLLSIGDRAR-IQLINGASM--EGHVDSIARGIYDRDNPQSRELTADVNPTFNWV 280 Query: 314 KVVQRVPVRIALD--PEQVRAHPLRIGLSTTATVDIHGDGR 352 ++ QR+PVRI LD PE V L GL+ T ++ G G+ Sbjct: 281 RLAQRIPVRIQLDKVPENVL---LASGLTCTVVLE-PGSGK 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.358 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 926,436 Number of extensions: 34419 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 6 Length of query: 406 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 319 Effective length of database: 1,207,925 Effective search space: 385328075 Effective search space used: 385328075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)