BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0007.p (524 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 1034 0.0 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 417 e-118 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 215 7e-57 gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 142 8e-35 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 127 2e-30 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 1034 bits (2673), Expect = 0.0, Method: Compositional matrix adjust. Identities = 524/524 (100%), Positives = 524/524 (100%) Query: 1 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE Sbjct: 1 MATSPTPSQAYTPPPPLTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDE 60 Query: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV 120 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV Sbjct: 61 GTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARV 120 Query: 121 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 180 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI Sbjct: 121 LQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWI 180 Query: 181 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA 240 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA Sbjct: 181 FYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFA 240 Query: 241 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI 300 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI Sbjct: 241 SPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVI 300 Query: 301 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL 360 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL Sbjct: 301 LPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWL 360 Query: 361 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 420 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA Sbjct: 361 RSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGA 420 Query: 421 VGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF 480 VGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF Sbjct: 421 VGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITF 480 Query: 481 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSKGGAGAAEASAAH 524 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSKGGAGAAEASAAH Sbjct: 481 QAAMLGINDIFYVSAVIFIVIIPLIWIIKPSKGGAGAAEASAAH 524 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 417 bits (1073), Expect = e-118, Method: Compositional matrix adjust. Identities = 222/509 (43%), Positives = 321/509 (63%), Gaps = 10/509 (1%) Query: 17 LTGANLVLGTLSVSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISI 76 L+G LV + ++ A F+ VLD++IANV++ TI+G LG S +GT+VITS+A A AI++ Sbjct: 26 LSGGQLVGAGILLAAANFIAVLDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITV 85 Query: 77 PLTGWLTQRLGAVRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSL 136 PLTGWL R G VR+F+ S+++F L S LCG+A +L +L+A RVLQGL GP++PLSQ+L Sbjct: 86 PLTGWLANRFGTVRVFILSMVMFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTL 145 Query: 137 LLGAYPKSKSSFALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL 196 LL +PK K+ A+ LW MT +VAP+AGP LGG + D Y+WP+IF+IN+PV LI + Sbjct: 146 LLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGW 205 Query: 197 AIYRSRETPTHKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFA 256 + + ET KLPID++GL L+V WVAA Q+MLD+GK+ DWFAS IV L + A++ F Sbjct: 206 RMLKRYETKMSKLPIDRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFI 265 Query: 257 YFVVWELNNDHPVVDLRLFGRRNFAGGTIAISIGYGVFFGNLVILPQWLQQYLGYRSIDS 316 F++WEL +P+VDLR+F R F I + +G FFG++V+ P WLQ Y+GY + S Sbjct: 266 AFLIWELTERNPIVDLRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWS 325 Query: 317 GLSTAPIGIFAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILP 376 G +A GI AV+ P++ + +DARK+ + +LR+ + + P Sbjct: 326 GCISALTGILAVMTAPMVAKMSTTIDARKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWP 385 Query: 377 TLLQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRSIM 436 LQGI +FFVPLT L LS + E +AAGL SF R GA+ TSI T+W N + Sbjct: 386 LFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATY 445 Query: 437 HHAQLTEHATPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAV 496 HA+L+ + ++ + ++ G++AEQ L +T+ QA ML N IF ++AV Sbjct: 446 FHAELSGLVDRGG----DAIHTMMAS-GMSAEQATASLAQTVQNQAVMLSTNHIFMMAAV 500 Query: 497 IFIVIIPLIWII-KPSKGGAGAAEASAAH 524 F + +W+ KP++ A+ SAAH Sbjct: 501 SFSLAALAVWLAPKPTR----VADTSAAH 525 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 215 bits (547), Expect = 7e-57, Method: Compositional matrix adjust. Identities = 144/485 (29%), Positives = 248/485 (51%), Gaps = 8/485 (1%) Query: 30 SLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAV 89 +L M LD SI N ++PTI G +G S EGTW+ T++ A + IP++ WL + LG Sbjct: 23 TLGALMATLDISIVNSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLR 82 Query: 90 RLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFA 149 L +T+ LF L S LCGL+ TLP+++ RV QG G ++P + +++ P+S+ Sbjct: 83 VLLLTAASLFTLFSVLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVG 142 Query: 150 LALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTL---AIYRSRETPT 206 A++G TA++ PV GP +GGW+T++ +W + F+IN+PVG AA +TL AI R Sbjct: 143 NAMFGATAILGPVLGPVMGGWLTENVSWHYAFFINLPVG--AALITLLLVAIPYERPKFE 200 Query: 207 HKLPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNND 266 D G+A L + ++L++G+ WF+SP I L +++ F + ++ Sbjct: 201 QLAEADWFGIAGLALGLGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRAR 260 Query: 267 HPVVDLRLFGRRNFAGGTIAISIGYGV-FFGNLVILPQWLQQYLGYRSIDSGLSTAPIGI 325 HPV+ LRL R F G + +++ GV +G ++PQ+L GY S+ SG G+ Sbjct: 261 HPVIQLRLLRDRQF-GSVVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGV 319 Query: 326 FAVILMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTA 385 +++MP + LVD R F+ A ++ + + + + L++G+ T Sbjct: 320 PMILMMPFTPLLMRLVDIRIAVGTGFLLLAASAFIETDLSPLSYGGSFVASQLMRGVGTV 379 Query: 386 MFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWNNRSIMHHAQLTEHA 445 + + L + +PP + A+GL S R G++ + + R +H +L E Sbjct: 380 LAMLFLNQAAIRSVPPSQAGDASGLYSAARNLGGSLALAAIAVIQDQRLWLHSRRLEESL 439 Query: 446 TPYSPWLQETVNNLSSTLGLNAEQTAGMLERTITFQAAMLGINDIFYVSAVIFIVIIPLI 505 S +Q ++ LG + ++ TI QA + D+F++ V + + PL+ Sbjct: 440 NANSDLVQSSMTAQGHLLG-GQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLV 498 Query: 506 WIIKP 510 ++P Sbjct: 499 LFLRP 503 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 142 bits (357), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 124/413 (30%), Positives = 195/413 (47%), Gaps = 23/413 (5%) Query: 29 VSLAVFMNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGA 88 V+ FM LD++I N A+P ++ +LG V V+ ++ A P +GWL R G Sbjct: 58 VASGFFMQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGT 117 Query: 89 VRLFVTSVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSF 148 R++ ++LLF L S LC +A +LP+LLAARV+QGL ++P+ + +L A P ++ Sbjct: 118 RRMYFAAILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIA 177 Query: 149 ALALWGMTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHK 208 ALA + + PV GP LGGW+ +S +W WIF IN+PVG++ G L + ++ Sbjct: 178 ALASISIAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVL-YFLPDDSLAGT 236 Query: 209 LPIDKIGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNNDH- 267 D +G LL + AF + LD D + T ++ + + A W + H Sbjct: 237 PRFDLLGCVLLSACMVAFSLALDVPFD----SHRTGWSIALFVVAALTVLGYWRHASVHP 292 Query: 268 -PVVDLRLFGRRNFA---GGTIAISIG-YGVFFGNLVILPQWLQQYLGYRSIDSGLSTAP 322 P+ L LFG NF+ G + IG V F ++ LQ LGY + +G+ P Sbjct: 293 RPLFRLGLFGEPNFSIGLAGNLVCRIGSSAVPFLLPLL----LQVRLGYSPLHAGMVLLP 348 Query: 323 IGIFAVI----LMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTL 378 I I + PL+ RF D + + +G AI+ + + + V L Sbjct: 349 AAIAGTIAKPWIAPLVKRF--GYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIF 406 Query: 379 LQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN 431 G +M F + + L GL + L S V++ +G +I T N Sbjct: 407 --GASNSMQFAAMNSVTLKGLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 457 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 127 bits (320), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 109/414 (26%), Positives = 195/414 (47%), Gaps = 15/414 (3%) Query: 38 LDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVL 97 +DS++ + ++P ++ ++ V +TS+ + A+ IP++GW+ R G++ +F +++ Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 98 LFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTA 157 +F+L S LCG++ TLP +AAR LQG+ MVP+ + ++L + K AL+ + A Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 158 VVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHK----LPIDK 213 + PV GP LGG+IT + W WIF+INIP+ ++ G+ LA +R P K P+D Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISIL--GLYLA---TRFIPDLKAEQVAPLDL 175 Query: 214 IGLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNN-DHPVVDL 272 G L++ + +M+ S + ++A + F VW P++DL Sbjct: 176 KG--FLLSALGCSLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDL 233 Query: 273 RLFGRRNFAGGTIAISIGYGVFFGNL-VILPQWLQQYLGYRSIDSGLSTAPIGIFAVILM 331 L G S Y V G + +LP LQ GY SG+ T A+ + Sbjct: 234 ALLKIPTLRAGVFGGSF-YRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMK 292 Query: 332 PLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTAMFFVPL 391 + RFL R+V + + W + + Q + +++ L+ G ++ F L Sbjct: 293 TIAARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSL 352 Query: 392 TVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN-NRSIMHHAQLTEH 444 + + + R+ A ++S + +G ++ A + I HA L Sbjct: 353 NAISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQ 406 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.139 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,502,886 Number of extensions: 65287 Number of successful extensions: 426 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 413 Number of HSP's successfully gapped: 16 Length of query: 524 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 434 Effective length of database: 1,193,720 Effective search space: 518074480 Effective search space used: 518074480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)