BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0005.p (329 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 666 0.0 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 108 8e-25 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 101 1e-22 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 92 8e-20 gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspiri... 80 2e-16 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 666 bits (1719), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/329 (99%), Positives = 327/329 (99%) Query: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 Query: 61 SEQLIAQVLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLEVDCLDLV 120 SEQLIAQVLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNL VDCLDLV Sbjct: 61 SEQLIAQVLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLGVDCLDLV 120 Query: 121 QLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF 180 QLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF Sbjct: 121 QLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIFNIF 180 Query: 181 RQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGET 240 RQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGET Sbjct: 181 RQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGET 240 Query: 241 FSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAAS 300 FSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAAS Sbjct: 241 FSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAAS 300 Query: 301 ALPPITAEVMQALRELYLHDIAPYVHQRW 329 ALPPITAEVMQALR +YLHDIAPYVHQRW Sbjct: 301 ALPPITAEVMQALRAIYLHDIAPYVHQRW 329 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 108 bits (269), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 92/323 (28%), Positives = 149/323 (46%), Gaps = 24/323 (7%) Query: 3 SRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSE 62 +RR G G VS IG G + + ++ A + AL+ G++ +DTAD+YG +E Sbjct: 17 TRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNE 76 Query: 63 QLIAQVLKERGGERPFVATKAGRRLSPHV--ASGYNDR-AQLSAFIDRSLKNLEVDCLDL 119 +L+ + +K R ++ F+ATK G R P A G + A + ++ SLK L V+ +DL Sbjct: 77 ELLGRAIKGR-RDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQAVEGSLKRLGVETIDL 135 Query: 120 VQLH----CPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQI 175 H P EV G L LV AGKIR+ G+S +A V ++Q Sbjct: 136 YYQHRIDPATPIEV-----TMGVLADLVQAGKIRYIGLSEASAATLERAHRVHPVTALQS 190 Query: 176 IFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAF 235 ++++ + P + L + I +A PL G LTG + A DD R+ N Sbjct: 191 EYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDFRRSN------ 244 Query: 236 DVGETFSGVPYEVALAAVEKIRALVPQ-GVPMAQFALRWILMESGISTVIPGARNVAQAQ 294 F G + L EK++ + Q G +Q AL W++ + IPG + + Sbjct: 245 ---PRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLE 301 Query: 295 SNSAASALPPITAEVMQALRELY 317 N+ + + + E +QAL ++ Sbjct: 302 DNAGSVGV-KLAPEDLQALDAIF 323 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 92/316 (29%), Positives = 147/316 (46%), Gaps = 22/316 (6%) Query: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGR 60 M RR G++ +VS + G+ G Q++ + +A + GV+FIDTADVY G+ Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQ---TDLQESGRIVASAREHGVNFIDTADVYTKGK 57 Query: 61 SEQLIAQVLKERGGERP--FVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLEVDCLD 118 SE+++ ++L G+R +A+K G +S R+ + + L L+ D LD Sbjct: 58 SEEIVGKLL---AGQRHEWVLASKLGNAMSGKPNESNYSRSWIVRETENILTRLDTDYLD 114 Query: 119 LVQLHCPPTEVYYQQEVFGHLDALVAAGKIRHYGVSV---EKVEEALKAIEYPNVK---S 172 ++ +H + ++ V L L+ GKIR +GVS ++ E ++ E NV Sbjct: 115 ILYMHRDYQDENLEEAVQA-LGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLV 173 Query: 173 VQIIFNIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHG 232 Q +N+ ++P L + V+ P+A G+LTGK A D R G Sbjct: 174 CQPYYNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQD-----TRAG 228 Query: 233 EAFDVGETFSGVPYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQ 292 D + E L A + +GV + QFA W+L S IS+VI G R +AQ Sbjct: 229 RG-DKRMMETEFREESLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQ 287 Query: 293 AQSNSAASALPPITAE 308 + A+ L I+AE Sbjct: 288 MEDYYDAAEL-KISAE 302 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 91.7 bits (226), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 104/341 (30%), Positives = 158/341 (46%), Gaps = 53/341 (15%) Query: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQ-DAKAALHAALDAGVSFIDTADVY--- 56 M+ R GRT +VS I G +WG+ +++ DA A L A++ G++ ID A++Y Sbjct: 1 MQYRPLGRTDLKVSLITLGTM----TWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVP 56 Query: 57 ----GDGRSEQLIAQVLKERGG-ERPFVATK----AGRRLSP-HVASGYN--DRAQLSAF 104 G +E+ + LK+ G ++ +ATK A + +P HV G N DR L+ Sbjct: 57 PKAETQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEA 116 Query: 105 IDRSLKNLEVDCLDLVQLHCPPTEVY------YQ----------QEVFGHLDALVAAGKI 148 + SL+ L++D +DL QLH P V YQ +E L V AGKI Sbjct: 117 LHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKI 176 Query: 149 RHYGVSVEK---VEEALKAIEYPNVKSVQIIFNIFR--QRPISLFLQE-AQRRDIAVIAR 202 R G+S E V + L+A E ++ + I N + R + L E A R + ++A Sbjct: 177 RTIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAY 236 Query: 203 VPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRALVPQ 262 PLA G+L+GK A G + E FS A A + AL Q Sbjct: 237 SPLAFGMLSGKYLDGARPA----------GGRLTLFERFSRYTNAQAERATAEYVALARQ 286 Query: 263 -GVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASAL 302 G+ AQ AL ++ +++ I GA +AQ +SN ++ L Sbjct: 287 HGLDPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAEL 327 >gi|300309642|ref|YP_003773734.1| aldo/keto reductase [Herbaspirillum seropedicae SmR1] Length = 280 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 83/295 (28%), Positives = 134/295 (45%), Gaps = 56/295 (18%) Query: 8 RTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSEQLIAQ 67 R G V +G G W +G S +A+ AL A LD G+S IDTA++Y +G +E+++A+ Sbjct: 10 RNGQSVPILGQGTWNMGESPAQANAE--VRALQAGLDLGMSLIDTAEMYAEGGAERIVAK 67 Query: 68 VLKERGGERPFVATKAGRRLSPHVASGYNDRAQLSAFIDRSLKNLEVDCLDLVQLHCPPT 127 + R + V+ ++ PH AS R ++A + SLK L D +DL LH Sbjct: 68 AIAGRRDQVYLVS-----KVLPHNAS---KRGTIAA-CEASLKRLGTDRIDLYLLHW--R 116 Query: 128 EVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSV---QIIFNIFRQRP 184 + ++ LV+ GKI +GVS ++ +E P+ + Q+++N+ R+ Sbjct: 117 GAHPLAATIEGMETLVSQGKIGGWGVSNFDTDDIDDLLEEPHGEHYLCNQVLYNLSRRGI 176 Query: 185 ISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGV 244 L +AQ+ IAV+A P+ G + +H Sbjct: 177 EYDLLPQAQQAHIAVMAYSPIEQG------------------RILKH------------- 205 Query: 245 PYEVALAAVEKIRALVPQGVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAA 299 P ALA ++ AQ AL W+L + G+ IP A +VA Q+N A+ Sbjct: 206 PALTALAQAHRVTP--------AQIALAWVLRQPGV-VAIPKAADVAHVQANRAS 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 933,272 Number of extensions: 34411 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 6 Length of query: 329 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 243 Effective length of database: 1,212,660 Effective search space: 294676380 Effective search space used: 294676380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)