BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0012.p (311 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA2507 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit ... 323 3e-88 AGc4592 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 129 1e-29 Cgl2351 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 125 1e-28 Cgl2996 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 104 3e-22 Cgl1134 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit... 101 2e-21 >PA2507 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Pae Pseudomonas aeruginosa Length = 310 Score = 323 bits (828), Expect = 3e-88, Method: Compositional matrix adjust. Identities = 173/309 (55%), Positives = 219/309 (70%), Gaps = 4/309 (1%) Query: 1 MAAKIFSTPEVQDFLRLASGLDTEGGSPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVA 60 M KI T +VQ F ASG E G PRTK +V RIL D K IE++++T DE+W V Sbjct: 1 MTVKISQTADVQRFFEEASGQLNERGDPRTKALVRRILDDTAKLIEEMQVTPDEFWKAVD 60 Query: 61 YVNRLGAASEAGLLSPGLGLDRFLDMRMDAEDAALGISGGTPRTIEGPLYVAGAPVEQGF 120 Y+NRLG+ EAGLL+ GLGL+ +LD+ +DA+DA G++GGTPRTIEGPLYVAGAP+ G Sbjct: 61 YLNRLGSRQEAGLLAAGLGLEHYLDLLLDAQDAEAGLTGGTPRTIEGPLYVAGAPLSDGE 120 Query: 121 ARLDDGSDKNGQTIIMHGTVYGSDGKPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRR 180 AR+DDG D G + + G V G DG+P+AGA V+VWHANT+G YS+FD + Q +N+RR Sbjct: 121 ARMDDGRDA-GTVMFLQGRVSGPDGQPLAGAIVDVWHANTQGTYSYFDSS--QSEYNLRR 177 Query: 181 TIITDAQGRYKFRSIMPAAYGCPPAGPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQ 240 I TDA GRY+ RSI+P+ YGCP GPTQ LL++LGRHG RPAHIHFFV+A GHR LTTQ Sbjct: 178 RIRTDADGRYRARSIVPSGYGCPSDGPTQELLDRLGRHGQRPAHIHFFVSAPGHRHLTTQ 237 Query: 241 INIEGDPLIYDDFAYATREGLVPPLVERTDAASIKAEGLE-GPFAEIVFDINLTSLVNGA 299 IN+ GD ++DDFAYATR+GL+ L D A+ + G+E G FAE+ FD L + A Sbjct: 238 INLAGDRYLWDDFAYATRDGLIGDLRFNDDPAAARDRGVEGGRFAELDFDFQLQASPAPA 297 Query: 300 DNQVVDRPR 308 + RPR Sbjct: 298 AERRSQRPR 306 >AGc4592 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 295 Score = 129 bits (323), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 80/261 (30%), Positives = 126/261 (48%), Gaps = 13/261 (4%) Query: 28 PRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYVNRLGAAS-----EAGLLSPGLGLDR 82 PR K+I+ + L + ++++E T +E+ + ++ +G E L S LG+ Sbjct: 31 PRLKEIMAVVTRKLHEAVKEIEPTEEEWMKAIHFLTEVGQICNEWRQEWILFSDILGVSM 90 Query: 83 FLDMRMDAEDAALGISGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKNGQTIIMHGTVYG 142 +D + SG + T+ GP +VA AP A + D G+ +++ G + Sbjct: 91 LVDAINHRKP-----SGASESTVLGPFHVADAPEMPMGANI--CLDGKGEDMVVTGRILD 143 Query: 143 SDGKPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPAAYGC 202 ++G P+AGA+++VW AN +GFY G Q FN+R +T GRY FR+ P Y Sbjct: 144 TEGAPVAGARIDVWQANDEGFYD-VQQKGIQPDFNLRGVFVTGEDGRYWFRAAKPKYYPI 202 Query: 203 PPAGPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIEGDPLIYDDFAYATREGLV 262 P GP LL +GRH RPAH+H+ V+A+ L T I DP I D + +E L+ Sbjct: 203 PDDGPVGRLLRAMGRHPYRPAHLHYIVSAESFTTLVTHIFDPDDPYIRSDAVFGVKESLL 262 Query: 263 PPLVERTDAASIKAEGLEGPF 283 D + G G + Sbjct: 263 ANFDRVEDREKAQELGFAGDW 283 >Cgl2351 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 285 Score = 125 bits (314), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 79/246 (32%), Positives = 124/246 (50%), Gaps = 8/246 (3%) Query: 18 ASGLDTEGGSPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYVNRLGAASEAGLLSPG 77 A+ + ++ R I +L+ + + E+T +EY ++ +G E L Sbjct: 25 ANRVSSDTSKERANAIYVDLLAAIAQVAHKHEVTYEEYAVLKQWMIDVGEYGEWPLW--- 81 Query: 78 LGLDRFLDMRMDAEDAALGISGGTPRTIEGPLYVAGAPVEQGFARLD-DGSDKNGQTIIM 136 LD F++ ++ + GT +IEGP YV +P A + D+ +I Sbjct: 82 --LDVFVEHEIEEINYNRHDYTGTKGSIEGPYYVENSPKLPWDAEMPMRDKDRACTPLIF 139 Query: 137 HGTVYGSDGKPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIM 196 G V DG + GA+VE+WHA+ G+YS F P + +N+R TI+TD +GRYK +++ Sbjct: 140 EGQVTDLDGNGLDGAEVELWHADEDGYYSQFAPGIPE--WNLRGTIVTDEEGRYKIKTLQ 197 Query: 197 PAAYGCPPAGPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIEGDPLIYDDFAYA 256 PA Y P GPT + G H RPAH+H V+ G+R +TTQ+ EG + +D A A Sbjct: 198 PAPYQIPHDGPTGWFIESYGGHPWRPAHLHLRVSHPGYRTITTQLYFEGGEWVENDVATA 257 Query: 257 TREGLV 262 + LV Sbjct: 258 VKPELV 263 >Cgl2996 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 301 Score = 104 bits (259), Expect = 3e-22, Method: Compositional matrix adjust. Identities = 78/249 (31%), Positives = 116/249 (46%), Gaps = 18/249 (7%) Query: 27 SPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYVNRLGAAS-----EAGLLSPGLGLD 81 +PR KQ++ ++ L I D+ +T DE+ + ++ +G + E LLS LG Sbjct: 34 NPRLKQLMESLVVHLHDFIRDVRLTEDEWNYAIDFLTAVGHITDDKRQEFVLLSDTLGAS 93 Query: 82 RFLDMRMDAEDAALGISGGTPRTIEGPLYVAGAP-VEQGFARLDDGSDKNGQTIIMHGTV 140 M+ A + T T+ GP ++ AP VE G D GQ + GTV Sbjct: 94 ----MQTIAVNNE-AYENSTEATVFGPFFLDDAPEVELGG---DIAGGAQGQAAWIEGTV 145 Query: 141 YGSDGKPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPAAY 200 ++G P+ A++EVW + G Y D + R + TDA G Y+F + P Y Sbjct: 146 TDTEGNPVPNARIEVWECDEDGLY---DVQYADERMAGRAYMHTDANGDYRFWGLTPVPY 202 Query: 201 GCPPAGPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIEGDP-LIYDDFAYATRE 259 P GP +L +GR R AH+HF VTA R L T I +EGDP L D + ++ Sbjct: 203 PIPHDGPVGNMLKAVGRSPVRCAHLHFMVTAPELRTLVTHIFVEGDPQLEIGDSVFGVKD 262 Query: 260 GLVPPLVER 268 L+ E+ Sbjct: 263 SLIKKFEEQ 271 >Cgl1134 [Q] COG3485 Protocatechuate 3,4-dioxygenase beta subunit Cgl Corynebacterium glutamicum Length = 295 Score = 101 bits (252), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 78/261 (29%), Positives = 124/261 (47%), Gaps = 21/261 (8%) Query: 16 RLASGLDTEGGSPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYVNRLGAAS-----E 70 R+ + D+ +PR K ++ + L I D+ +T +E+ + ++ ++G + E Sbjct: 17 RVLASFDS-CENPRLKLVMKSLTVHLHDFIRDVRLTEEEWNYAIDFLTKVGHITDDKRQE 75 Query: 71 AGLLSPGLGLD-RFLDMRMDA-EDAALGISGGTPRTIEGPLYVAGAPVEQGFARLDDGSD 128 LLS LG + + + +A EDA T T+ GP +V AP+ Q + G+ Sbjct: 76 FVLLSDTLGASMQTIAVNNEAYEDA-------TEATVFGPFFVDDAPLVQNGDDIAFGA- 127 Query: 129 KNGQTIIMHGTVYGSDGKPMAGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQG 188 GQ + GTV ++G P+ A++EVW + G Y D + R + +D G Sbjct: 128 -VGQPAWVEGTVKDTEGNPIPNARIEVWECDEDGLY---DVQYADERSAGRAHLYSDENG 183 Query: 189 RYKFRSIMPAAYGCPPAGPTQGLLNQLGRHGNRPAHIHFFVTADGHRKLTTQINIEGDP- 247 Y F + P Y P GP +L +GR R AH+HF VTA R L T I +EGDP Sbjct: 184 EYHFWGLTPVPYPIPHDGPVGQMLQAVGRSPVRCAHLHFMVTAPEKRTLVTHIFVEGDPQ 243 Query: 248 LIYDDFAYATREGLVPPLVER 268 L D + ++ L+ VE+ Sbjct: 244 LEIGDSVFGVKDSLIKKFVEQ 264