BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0394.p (235 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 120 3e-27 VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pa... 93 5e-19 fliH [NU] COG1317 Flagellar biosynthesis/type III secretory path... 89 8e-18 ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pat... 88 2e-17 ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 88 2e-17 >RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Rso Ralstonia solanacearum Length = 248 Score = 120 bits (300), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 65/175 (37%), Positives = 100/175 (57%), Gaps = 12/175 (6%) Query: 60 REAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNEIIQHFRQIAVNFSTEVSQCSEK 119 R+ R++GFA+G + G + GR +G ++ +++ ++Q R+ + Q + Sbjct: 68 RDEGRRDGFAQGFQDGFEQGRRQG-----EEDSRQIAALMQSVREA-------IDQMNGS 115 Query: 120 MAPELLDLALDLAKSMLKTALNVRPELILPTVSAAIHALPVLQLPASLTLHPEDAALVRK 179 +A EL+ LAL LA+ L+ AL +PE ILP V + P PA L +H +DA L+R+ Sbjct: 116 VAEELVGLALQLAQQFLRGALRAQPERILPLVREVLGDAPTAPAPAMLRVHADDAELIRQ 175 Query: 180 HLGEELAQQGWKIEEDAHLDRGGCRVETRTNQIDATTPTRWQRLGESLSKQLDWL 234 LG ELA GW + DA ++RGGCRV+TR + DAT TRW L +L + W+ Sbjct: 176 ALGTELAAAGWTLVVDAAIERGGCRVQTRFGETDATLQTRWAELTRALGRDTAWI 230 >VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Vch Vibrio cholerae Length = 270 Score = 93.2 bits (230), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 65/222 (29%), Positives = 113/222 (50%), Gaps = 31/222 (13%) Query: 11 MTPYQRWELASFNEEPEPEPQ-------PEMLPEEEEFLEEPPPVPQ--LTAEQLDAIRE 61 +TP QRW L + E + P +P +EP V + + E++ IR Sbjct: 23 VTP-QRWGLPDYGAESNKAAKQTAFNYDPGWIPN----FDEPEQVVEHEFSEEEIALIRT 77 Query: 62 AARQEGF----AEGREHGLQAGRSEGYVAGLQQGQQE------------VNEIIQHFRQI 105 AA+QEGF AEG + G + G++EG+ AG Q+GQ + + E ++ F + Sbjct: 78 AAQQEGFEAGQAEGYQQGFEQGKAEGFQAGHQEGQTQGYQDGVAEGQALIQEQVKTFMAL 137 Query: 106 AVNFSTEVSQCSEKMAPELLDLALDLAKSMLKTALNVRPELILPTVSAAIHALPVLQLPA 165 A F+ + + ++ +L+D+ L L K ++ + P++IL TV A++ ALP+ Sbjct: 138 ANQFAQPLDLLNAQVEKQLVDMVLALTKEVVHVEVQTNPQVILDTVKASVEALPIAGHAI 197 Query: 166 SLTLHPEDAALVRKHLGE-ELAQQGWKIEEDAHLDRGGCRVE 206 +L L+PED ++R+ GE E+ + W + + L RG ++E Sbjct: 198 TLKLNPEDVEIIRQAYGEQEIETRNWTLLSEPALSRGDVQIE 239 >fliH [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Eco Escherichia coli K12 Length = 235 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 66/227 (29%), Positives = 99/227 (43%), Gaps = 5/227 (2%) Query: 1 MRAKIIPKEEMTPYQRWELASFNEEPEPEPQPEMLPEEEEFLEEPPPVPQLTAEQLDAIR 60 M A IP + P++ W P+ E P + PEE E P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTWTPDDLAP-PQAEFVPIVEPEETIIEEAEPSLEQQLAQLQMQAH 59 Query: 61 EAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTEVSQC 116 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T + Sbjct: 60 EQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLDAL 119 Query: 117 SEKMAPELLDLALDLAKSMLKTALNVRPELILPTVSAAIHALPVLQLPASLTLHPEDAAL 176 +A L+ +AL+ A+ ++ V ++ + + P+ L +HP+D Sbjct: 120 DSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQR 179 Query: 177 VRKHLGEELAQQGWKIEEDAHLDRGGCRVETRTNQIDATTPTRWQRL 223 V LG L+ GW++ D L GGC+V +DA+ TRWQ L Sbjct: 180 VDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226 >ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein EcZ Escherichia coli O157:H7 EDL933 Length = 235 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 67/231 (29%), Positives = 101/231 (43%), Gaps = 13/231 (5%) Query: 1 MRAKIIPKEEMTPYQRWELASFNEEPEPEPQPEMLP---EEEEFLEEP-PPVPQLTAEQL 56 M A IP + P++ W + PQ E +P EE +EE P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTW-----TPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQLAQLQ 55 Query: 57 DAIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTE 112 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T Sbjct: 56 MQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTT 115 Query: 113 VSQCSEKMAPELLDLALDLAKSMLKTALNVRPELILPTVSAAIHALPVLQLPASLTLHPE 172 + +A L+ +AL+ A+ ++ V ++ + + P+ L +HP+ Sbjct: 116 LDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPD 175 Query: 173 DAALVRKHLGEELAQQGWKIEEDAHLDRGGCRVETRTNQIDATTPTRWQRL 223 D V LG L+ GW++ D L GGC+V +DA+ TRWQ L Sbjct: 176 DLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226 >ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Ecs Escherichia coli O157:H7 Length = 235 Score = 87.8 bits (216), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 67/231 (29%), Positives = 101/231 (43%), Gaps = 13/231 (5%) Query: 1 MRAKIIPKEEMTPYQRWELASFNEEPEPEPQPEMLP---EEEEFLEEP-PPVPQLTAEQL 56 M A IP + P++ W + PQ E +P EE +EE P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTW-----TPDDLAPPQAEFVPMVESEETIIEEAEPSLEQQLAQLQ 55 Query: 57 DAIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTE 112 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T Sbjct: 56 MQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTT 115 Query: 113 VSQCSEKMAPELLDLALDLAKSMLKTALNVRPELILPTVSAAIHALPVLQLPASLTLHPE 172 + +A L+ +AL+ A+ ++ V ++ + + P+ L +HP+ Sbjct: 116 LDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPD 175 Query: 173 DAALVRKHLGEELAQQGWKIEEDAHLDRGGCRVETRTNQIDATTPTRWQRL 223 D V LG L+ GW++ D L GGC+V +DA+ TRWQ L Sbjct: 176 DLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226