BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0349.p (324 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3892 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomo... 359 4e-99 PA2430 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomo... 257 2e-68 AGpA806 [V] COG1566 Multidrug resistance efflux pump Atu Agrobac... 235 1e-61 ydhJ [V] COG1566 Multidrug resistance efflux pump Eco Escherichi... 231 2e-60 Z2659 [V] COG1566 Multidrug resistance efflux pump EcZ Escherich... 229 5e-60 >PA3892 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomonas aeruginosa Length = 302 Score = 359 bits (922), Expect = 4e-99, Method: Compositional matrix adjust. Identities = 180/313 (57%), Positives = 234/313 (74%), Gaps = 28/313 (8%) Query: 1 MPVKSIFRVLITTAIFLLAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALP 60 M +KS+ +L T I L+A+ + +LW +YM +PWTRDGRVRADV+N+A DVSG VV +P Sbjct: 1 MTLKSVISLLATLLILLVAVFIGRTLWVNYMDTPWTRDGRVRADVINVAADVSGIVVDVP 60 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKADLDVRKAEVEARRADLNMRRAQA 120 VRDNQLVKKGDLLM+IDP Y +A++QAE+ V +RKA L+ MR+ A Sbjct: 61 VRDNQLVKKGDLLMQIDPDHYRIAVKQAESLVASRKATLE--------------MRQLNA 106 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQFQGAQAQYQAALSQRDTAKLNLER 180 RRRA++D +VVSRE+R+DA + A+ A E A+AQ D A+LNLER Sbjct: 107 RRRAEMDEMVVSRESRDDAHNTAAAAMADYEQAKAQL--------------DAARLNLER 152 Query: 181 TKVFSPVDGYITNLSVRRGDYAAAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRAKIQ 240 T+V + VDGY+TNL+V RGDYA G AK+AVID +S+WVY YFEETKLP + GD +Q Sbjct: 153 TRVVAQVDGYVTNLNVHRGDYARVGEAKMAVIDKNSYWVYGYFEETKLPYIREGDPVDMQ 212 Query: 241 LINGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPDNVL 300 L++G ++GHV+SIARGIYDRDNP+SRELTADVNPTFNWVRLAQR+PVR+H+D+VPD VL Sbjct: 213 LMSGEHLKGHVESIARGIYDRDNPESRELTADVNPTFNWVRLAQRVPVRVHIDEVPDGVL 272 Query: 301 LASGLTCTVVLEP 313 L++G+TCTV+++P Sbjct: 273 LSAGITCTVIVKP 285 >PA2430 [V] COG1566 Multidrug resistance efflux pump Pae Pseudomonas aeruginosa Length = 334 Score = 257 bits (657), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 137/290 (47%), Positives = 193/290 (66%), Gaps = 4/290 (1%) Query: 27 WQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALPVRDNQLVKKGDLLMEIDPARYTLALQ 86 W+ Y +PWTRD RVRADVV ++ DVSG + LP+ DNQ V+KG+LL+ IDPARY LA+ Sbjct: 28 WERYTRTPWTRDARVRADVVTLSADVSGLITRLPLSDNQPVEKGELLLSIDPARYELAVL 87 Query: 87 QAEAAVTARKADLDVRKAEVEARRADLNMRRAQARRRADVDAL-VVSREAREDAGSQVTA 145 +AE AV +A L +A +EA +A L RR++A+RR+ + ++S E E A + V+ Sbjct: 88 RAERAVNVARAALGESRAAIEASQAQLRQRRSEAQRRSALQKRSMLSVEENEKAQTDVSL 147 Query: 146 SQAQLEAAQAQFQGAQAQYQAALSQRDTAKLNLERTKVFSPVDGYITNLSVRRGDYAAAG 205 +QA+L QA AQA + A + A+L+LERT+V +PV GY+TNL R GDYA AG Sbjct: 148 AQAELLRNQASLGLAQANVELAEAALQQARLDLERTQVRAPVSGYVTNLQTREGDYAHAG 207 Query: 206 AAKIAVIDSHSFWVYAYFEETKLPLLSIGDRAKIQLINGASMEGHVDSIARGIYDRDNPQ 265 +A++D SF+V YFEETKLP + +G RA+++L++G +G V SIA I DR+N + Sbjct: 208 VPLLALVDRDSFYVSGYFEETKLPGIHVGSRARVELMSGERFDGRVQSIAHAITDRENAE 267 Query: 266 SRELTADVNPTFNWVRLAQRIPVRIHLDKV--PDNVLLASGLTCTVVLEP 313 L A++NP++ WV+LAQRIPVRI +D N L A G+T T+ + P Sbjct: 268 GSRLLANINPSYTWVKLAQRIPVRIAIDPAYRQRNTLRA-GVTATIRVLP 316 >AGpA806 [V] COG1566 Multidrug resistance efflux pump Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 392 Score = 235 bits (599), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 136/306 (44%), Positives = 181/306 (59%), Gaps = 28/306 (9%) Query: 8 RVLITTAIFLLAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALPVRDNQLV 67 RVL+T ++ A ++ W LW ++ +PWTRD VRAD+V +APD+SG V + V DN LV Sbjct: 111 RVLLTLSMSSCAAVMGWHLWSFHIGTPWTRDAHVRADIVRLAPDISGPVTEVLVSDNALV 170 Query: 68 KKGDLLMEIDPARYTLALQQAEAAVTARKADLDVRKAEVEARRADLNMRRAQARRRADVD 127 +K L I+ R+ LAL++AEA VT KA D K + E R Q Sbjct: 171 EKDAPLFRIEKDRFELALREAEAQVTGAKAGTDAAKVDFE---------RYQR------- 214 Query: 128 ALVVSREAREDAGSQVTASQAQLEAAQAQFQGAQAQYQAALSQRDTAKLNLERTKVFSPV 187 + S+EA AS + + A+ Q A+A YQ+AL RD AKLNLERT V +P Sbjct: 215 --LASKEA---------ASVRERQHAETQMYQAEAAYQSALVARDLAKLNLERTTVRAPA 263 Query: 188 DGYITNLSVRRGDYAAAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRAKIQLINGA-S 246 G ITN S+R+G+Y AG A +++ S +V YFEETKLP + IGDR KI ++ S Sbjct: 264 SGAITNFSLRKGNYVLAGNAVGVLVEKESVYVAGYFEETKLPRIHIGDRVKIDIMGEPES 323 Query: 247 MEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPDNVLLASGLT 306 GHV +A GI DR+ + A V PTF+WVRLAQRIPVRI +D VP+NV L +G T Sbjct: 324 AIGHVAGVAAGIEDRERSDTAGSLASVAPTFSWVRLAQRIPVRIEIDHVPNNVRLIAGRT 383 Query: 307 CTVVLE 312 T+ +E Sbjct: 384 ATIAIE 389 >ydhJ [V] COG1566 Multidrug resistance efflux pump Eco Escherichia coli K12 Length = 299 Score = 231 bits (589), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 132/312 (42%), Positives = 183/312 (58%), Gaps = 30/312 (9%) Query: 1 MPVKSIFRVLITTAIFLLAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALP 60 M +K+I + T + ++A+L W LW +YM SPWTRDG++RA+ V+I P VSG++V L Sbjct: 15 MSIKTI-KYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQVSITPQVSGRIVELN 73 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKADLDVRKAEVEARRADLNMRRAQA 120 ++DNQLV GDLL+ ID + +A A+A + KA D+ KA E A Sbjct: 74 IKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLA--KAQSDLAKANNE------------A 119 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQFQGAQAQYQAALSQRDTAKLNLER 180 RR + +S E + A V A QA ++AAQA + QAQ+Q L + Sbjct: 120 NRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLK--QAQWQ------------LAQ 165 Query: 181 TKVFSPVDGYITNLSVRRGDYAAAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRAKIQ 240 T++ +PV G++TNL+ R GDYA G A++DSHSF+V YFEETKL + G A+I Sbjct: 166 TEIRAPVSGWVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQIT 225 Query: 241 LI-NGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPDNV 299 L + +++GHV SI R IYD+ L DV P WVRLAQR+PVR LDKVP +V Sbjct: 226 LYSDNKTLQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDV 285 Query: 300 LLASGLTCTVVL 311 L SG TC++ + Sbjct: 286 TLVSGTTCSIAV 297 >Z2659 [V] COG1566 Multidrug resistance efflux pump EcZ Escherichia coli O157:H7 EDL933 Length = 299 Score = 229 bits (585), Expect = 5e-60, Method: Compositional matrix adjust. Identities = 131/312 (41%), Positives = 182/312 (58%), Gaps = 30/312 (9%) Query: 1 MPVKSIFRVLITTAIFLLAILLAWSLWQHYMHSPWTRDGRVRADVVNIAPDVSGQVVALP 60 M +K+I + T + ++A+L W LW +YM SPWTRDG++RA+ V+I P VSG++V L Sbjct: 15 MSIKTI-KYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQVSITPQVSGRIVELN 73 Query: 61 VRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKADLDVRKAEVEARRADLNMRRAQA 120 ++DNQLV GDLL+ ID + +A A+A + KA D+ KA E A Sbjct: 74 IKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLA--KAQSDLAKANNE------------A 119 Query: 121 RRRADVDALVVSREAREDAGSQVTASQAQLEAAQAQFQGAQAQYQAALSQRDTAKLNLER 180 RR + +S E + A V A QA ++AAQA + Q Q+Q L + Sbjct: 120 NRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLK--QTQWQ------------LAQ 165 Query: 181 TKVFSPVDGYITNLSVRRGDYAAAGAAKIAVIDSHSFWVYAYFEETKLPLLSIGDRAKIQ 240 T++ +PV G++TNL+ R GDYA G A++DSHSF+V YFEETKL + G A+I Sbjct: 166 TEIRAPVSGWVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQIT 225 Query: 241 LI-NGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRIHLDKVPDNV 299 L + +++GHV SI R IYD+ L DV P WVRLAQR+PVR LDKVP +V Sbjct: 226 LYSDNKTLQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDV 285 Query: 300 LLASGLTCTVVL 311 L SG TC++ + Sbjct: 286 TLVSGTTCSIAV 297