BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0270.p (248 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value NMA1016 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 313 2e-85 RSc1401_2 [J] COG1187 16S rRNA uridine-516 pseudouridylate synth... 313 3e-85 NMB0806 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthas... 312 4e-85 CC0855 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase... 177 1e-44 PA1110 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase... 172 4e-43 >NMA1016 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases NmA Neisseria meningitidis Z2491 Length = 252 Score = 313 bits (803), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 156/246 (63%), Positives = 193/246 (78%), Gaps = 5/246 (2%) Query: 3 EPVRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAEQA 62 + VRLSKRM++LGLCSRREAD +IE+GWV V+G+ +VLG KV P ++ + ++A +QA Sbjct: 12 DAVRLSKRMAQLGLCSRREADGYIEQGWVTVNGKT-AVLGQKVSPADRIELNKKAHEQQA 70 Query: 63 QRVTILINKPMGYVSGQAEDGYKPAMVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRLDI 122 R+TIL+NKP+GYVS QAE GYK A L+ ++RW D +RF P+ L PAGRLDI Sbjct: 71 ARITILLNKPVGYVSAQAEKGYKSAAELITPENRWEGDTGRIRFDPKHKIGLAPAGRLDI 130 Query: 123 DSVGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPESDLKRLNHGLIMDGKPL 182 DSVGLLVLTQDGRIAKQLIGE+S +KEYLVRV+ GKL E L LNHGL +DG+ L Sbjct: 131 DSVGLLVLTQDGRIAKQLIGENSGSEKEYLVRVR----GKLDEKGLALLNHGLSLDGEKL 186 Query: 183 RPAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELPTGQWRYL 242 RPA+V WQNEDQLRF L++GKKRQIRRMC++VGL+V+GLKR+R+G VKLG LP G+WRYL Sbjct: 187 RPAQVEWQNEDQLRFVLKQGKKRQIRRMCELVGLRVVGLKRIRMGKVKLGRLPPGKWRYL 246 Query: 243 RPDEKF 248 P E F Sbjct: 247 APGESF 252 >RSc1401_2 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Rso Ralstonia solanacearum Length = 261 Score = 313 bits (801), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 157/253 (62%), Positives = 191/253 (75%), Gaps = 9/253 (3%) Query: 5 VRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAEQAQR 64 VRLSKRMSELGLCSRREADEWI +GWV VDG+ V+ LGS+V P ++ + A +EQ +R Sbjct: 9 VRLSKRMSELGLCSRREADEWIPRGWVLVDGEPVTELGSRVRPDAEIEILPAARSEQGER 68 Query: 65 VTILINKPMGYVSGQAEDGYKPAMVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRLDIDS 124 VT+L++KP+GYVSGQAEDGY+PA L +++W ED T F Q K L PAGRLDIDS Sbjct: 69 VTVLLHKPVGYVSGQAEDGYEPAAALFTAENQWEEDPTRKHFAAWQHKGLAPAGRLDIDS 128 Query: 125 VGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGK---------LPESDLKRLNHGL 175 GLLVLTQDGR+A+ LIGEDS V+KEYLVRV + P PE L+ L HGL Sbjct: 129 TGLLVLTQDGRVARHLIGEDSTVEKEYLVRVVWNAPQGPVEHDISKVFPEDLLEMLRHGL 188 Query: 176 IMDGKPLRPAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELP 235 +DG+ L+PAKV WQNE+QLRF LREGKKRQIRRMC+ VGL+V+GLKR+R+G V LG+LP Sbjct: 189 SLDGEALKPAKVSWQNEEQLRFVLREGKKRQIRRMCEQVGLEVVGLKRIRMGSVVLGDLP 248 Query: 236 TGQWRYLRPDEKF 248 G+WR+L EKF Sbjct: 249 VGKWRFLGRFEKF 261 >NMB0806 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Nme Neisseria meningitidis MC58 Length = 252 Score = 312 bits (800), Expect = 4e-85, Method: Compositional matrix adjust. Identities = 155/246 (63%), Positives = 192/246 (78%), Gaps = 5/246 (2%) Query: 3 EPVRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAEQA 62 + VRLSKRM++LGLCSRREAD +IE+GWV V+G+ +VLG KV P ++ + ++A +QA Sbjct: 12 DAVRLSKRMAQLGLCSRREADGYIEQGWVTVNGKT-AVLGQKVSPADRIELNKKAHEQQA 70 Query: 63 QRVTILINKPMGYVSGQAEDGYKPAMVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRLDI 122 R+TIL+NKP+GYVS QAE GYK A L+ ++ W D +RF P+ L PAGRLDI Sbjct: 71 ARITILLNKPVGYVSAQAEKGYKSAAELITPENHWEGDTGRIRFDPKHKIGLAPAGRLDI 130 Query: 123 DSVGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPESDLKRLNHGLIMDGKPL 182 DSVGLLVLTQDGRIAKQLIGE+S +KEYLVRV+ GKL E L LNHGL +DG+ L Sbjct: 131 DSVGLLVLTQDGRIAKQLIGENSGSEKEYLVRVR----GKLDEKGLALLNHGLSLDGEKL 186 Query: 183 RPAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELPTGQWRYL 242 RPA+V WQNEDQLRF L++GKKRQIRRMC++VGL+V+GLKR+R+G VKLG LP G+WRYL Sbjct: 187 RPAQVEWQNEDQLRFVLKQGKKRQIRRMCELVGLRVVGLKRIRMGKVKLGRLPPGKWRYL 246 Query: 243 RPDEKF 248 P E F Sbjct: 247 APGESF 252 >CC0855 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Ccr Caulobacter vibrioides Length = 255 Score = 177 bits (450), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 107/246 (43%), Positives = 156/246 (63%), Gaps = 6/246 (2%) Query: 2 TEPVRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAEQ 61 EP R++K +++ G+CSRREA++ I G V +DG+VV +G K+ P Q +T+ +A+A+ Sbjct: 10 AEPQRVNKWLAQSGVCSRREAEQLISDGLVSIDGEVVEDVGRKIQPGQTLTLADRATAKL 69 Query: 62 AQRVTILINKPMGYVSGQAEDGYKPAMVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRLD 121 T L++KP G VS Q + G PA L+ + W + + P+ + P GRLD Sbjct: 70 DAVPTYLVHKPRGVVSSQPDPGQVPAARLLTRANLWGDARGVA--IPQSGDLIPPIGRLD 127 Query: 122 IDSVGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPESDLKRLNHGLIMDGKP 181 DS GLL+L+QDG +AK +IG S ++KEY V V G+L E L+ L GL +DG+ Sbjct: 128 KDSRGLLLLSQDGVVAKAVIGPQSELEKEYRVAVM----GELTEEKLELLRFGLELDGRE 183 Query: 182 LRPAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELPTGQWRY 241 LRPA+V ++ +L F LREG+ RQIRRMC++V LKV+ L RVR+G + LG++P G WR Sbjct: 184 LRPAEVEVISDQRLSFVLREGRNRQIRRMCELVDLKVVDLFRVRVGPLDLGDMPEGHWRL 243 Query: 242 LRPDEK 247 L E+ Sbjct: 244 LTAAER 249 >PA1110 [J] COG1187 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases Pae Pseudomonas aeruginosa Length = 236 Score = 172 bits (437), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 99/248 (39%), Positives = 146/248 (58%), Gaps = 12/248 (4%) Query: 1 MTEPVRLSKRMSELGLCSRREADEWIEKGWVRVDGQVVSVLGSKVLPHQKVTVERQASAE 60 MT+P+RLSKR++EL CSRREA+ +IE GWV VDG+V+ KVL Q+V + A AE Sbjct: 1 MTDPIRLSKRLAELTACSRREAELYIEGGWVSVDGEVIEEPQFKVL-DQRVELLPGARAE 59 Query: 61 QAQRVTILINKPMGYVSGQAEDGYKPAMVLVNEQSRWAEDQTEMRFHPRQLKSLVPAGRL 120 + T+L++KP G+ E L+ RW++D + +R + P L Sbjct: 60 TIEPATLLLHKPAGWRHDDFEG-------LLAAGRRWSDDPSPLRALKKHFARQRPTLAL 112 Query: 121 DIDSVGLLVLTQDGRIAKQLIGEDSAVDKEYLVRVQYTRPGKLPESDLKRLNHGLIMDGK 180 D ++ GL+V +Q + ++L+ + + +++EYLV V G L L+RL HGL G+ Sbjct: 113 DSEASGLVVFSQQHGVLRKLVDDGARLEQEYLVEVA----GDLAAGGLERLRHGLAYQGR 168 Query: 181 PLRPAKVVWQNEDQLRFTLREGKKRQIRRMCDMVGLKVIGLKRVRIGGVKLGELPTGQWR 240 L P KV WQNE LRF L++ Q+R MC+ GL+V ++R+RIG + L LP G+WR Sbjct: 169 RLSPCKVSWQNESHLRFALKDVLPGQLRFMCEREGLEVRSIRRLRIGALSLARLPLGEWR 228 Query: 241 YLRPDEKF 248 YL E+F Sbjct: 229 YLGLHERF 236