BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0103.p (220 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5203 [H] COG2918 Gamma-glutamylcysteine synthetase Pae Pseudom... 286 3e-77 VC0556 [H] COG2918 Gamma-glutamylcysteine synthetase Vch Vibrio ... 196 5e-50 YPO3301 [H] COG2918 Gamma-glutamylcysteine synthetase Ype Yersin... 193 2e-49 STM2818 [H] COG2918 Gamma-glutamylcysteine synthetase Sty Salmon... 186 4e-47 ZgshA [H] COG2918 Gamma-glutamylcysteine synthetase EcZ Escheric... 182 4e-46 >PA5203 [H] COG2918 Gamma-glutamylcysteine synthetase Pae Pseudomonas aeruginosa Length = 527 Score = 286 bits (731), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 149/220 (67%), Positives = 170/220 (77%), Gaps = 3/220 (1%) Query: 1 MTDLLTRRLQQLSQPHHLALLGQGLRGVERETLRVDGQGHLARTPHPPALGSALTNDLIT 60 M+DLL+RRL L +L LL + L G+ERE LRVD G LA TPHP ALGS LT+ IT Sbjct: 1 MSDLLSRRLALLGAAANLPLLTECLHGIERECLRVDSDGKLALTPHPRALGSTLTHPQIT 60 Query: 61 TDYSESLLEFITPAMPDIADALQRLDEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAW 120 TDYSE+LLEFITP D+AD L L+ IHRFA +KLDGE+LW+ SMPC LP E+ IP+A Sbjct: 61 TDYSEALLEFITPTETDVADTLGDLERIHRFASSKLDGEYLWSPSMPCELPDEESIPIAR 120 Query: 121 YGSSHIGMLKHVYRRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRD 180 YGSS IG LK+VYR+GLALRYG+TMQCIAGIHYNFSL E LW +LR + + LS RD Sbjct: 121 YGSSMIGRLKYVYRKGLALRYGKTMQCIAGIHYNFSLPERLWPLLRQAEGS---ELSERD 177 Query: 181 YQSERYIALIRNFRRYSWLLMYLFGASPAVATNFLRGRPS 220 YQS YIALIRNFRRYSWLLMYLFGASPA+ FLRGRPS Sbjct: 178 YQSAAYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRPS 217 >VC0556 [H] COG2918 Gamma-glutamylcysteine synthetase Vch Vibrio cholerae Length = 524 Score = 196 bits (497), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 96/195 (49%), Positives = 131/195 (67%), Gaps = 4/195 (2%) Query: 26 RGVERETLRVDGQGHLARTPHPPALGSALTNDLITTDYSESLLEFITPAMPDIADALQRL 85 RG+ERE+LR +G L +TPHP ALG+ALT+ ITTD++ESLLEFITP ++ L ++ Sbjct: 23 RGLERESLRYTPEGALTQTPHPKALGAALTHRWITTDFAESLLEFITPVSHEVDTLLAQM 82 Query: 86 DEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAWYGSSHIGMLKHVYRRGLALRYGRTM 145 +IH FA TKL E LW SMPC + E+ I LA YGSS+ G +K +YR GL RYG M Sbjct: 83 SDIHHFAQTKLGDEKLWPLSMPCYVGSEEGIVLAQYGSSNTGKMKTLYREGLKRRYGSLM 142 Query: 146 QCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRDYQSERYIALIRNFRRYSWLLMYLFG 205 Q I+G+H+NFS E W+ L + +R+ +S Y +IRN+ R+ WL+ YLFG Sbjct: 143 QIISGVHFNFSFPESFWDALFGEQEES----ARQASKSAAYFGVIRNYYRFGWLIPYLFG 198 Query: 206 ASPAVATNFLRGRPS 220 ASPA+ ++FL+GR + Sbjct: 199 ASPALCSSFLQGRKT 213 >YPO3301 [H] COG2918 Gamma-glutamylcysteine synthetase Ype Yersinia pestis Length = 519 Score = 193 bits (491), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 99/195 (50%), Positives = 128/195 (65%), Gaps = 4/195 (2%) Query: 26 RGVERETLRVDGQGHLARTPHPPALGSALTNDLITTDYSESLLEFITPAMPDIADALQRL 85 RG+ERETLRV GHLA T HP +LG+ALT+ ITTD++E+LLEFITP DI L L Sbjct: 24 RGIERETLRVTADGHLASTGHPESLGAALTHQWITTDFAEALLEFITPVDGDIDHLLTFL 83 Query: 86 DEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAWYGSSHIGMLKHVYRRGLALRYGRTM 145 +IHR+ KL E +W SMPC + E +I LA YGSS+IG K +YR GL RYG M Sbjct: 84 RDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALM 143 Query: 146 QCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRDYQSERYIALIRNFRRYSWLLMYLFG 205 Q I+G+HYNFSL + W+ V S ++ S Y LIRN+ R+ W++ YLFG Sbjct: 144 QTISGVHYNFSLPLEFWQAWAG----VTDEQSGKEEISAGYFRLIRNYYRFGWVIPYLFG 199 Query: 206 ASPAVATNFLRGRPS 220 ASPA+ ++FL+GR + Sbjct: 200 ASPAICSSFLQGRET 214 >STM2818 [H] COG2918 Gamma-glutamylcysteine synthetase Sty Salmonella typhimurium LT2 Length = 518 Score = 186 bits (471), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 94/195 (48%), Positives = 126/195 (64%), Gaps = 4/195 (2%) Query: 26 RGVERETLRVDGQGHLARTPHPPALGSALTNDLITTDYSESLLEFITPAMPDIADALQRL 85 RG+ERETLRV+ G LA T HP ALGSALT+ ITTD++E+LLEFITP DI L + Sbjct: 24 RGLERETLRVNADGTLATTGHPEALGSALTHKWITTDFAEALLEFITPVDGDIQHMLTFM 83 Query: 86 DEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAWYGSSHIGMLKHVYRRGLALRYGRTM 145 ++HR+ KL E +W SMPC + +I LA YG+S+ G K +YR GL RYG M Sbjct: 84 RDLHRYTARKLGDERMWPLSMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALM 143 Query: 146 QCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRDYQSERYIALIRNFRRYSWLLMYLFG 205 Q I+G+HYNFSL W+ V + ++ S Y LIRN+ R+ W++ YLFG Sbjct: 144 QTISGVHYNFSLPMAFWQA----KCGVTEGEAAKEKISAGYFRLIRNYYRFGWVIPYLFG 199 Query: 206 ASPAVATNFLRGRPS 220 ASPA+ ++FL+G+P+ Sbjct: 200 ASPAICSSFLQGKPT 214 >ZgshA [H] COG2918 Gamma-glutamylcysteine synthetase EcZ Escherichia coli O157:H7 EDL933 Length = 518 Score = 182 bits (463), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 96/207 (46%), Positives = 132/207 (63%), Gaps = 5/207 (2%) Query: 14 QPHHLALLGQGLRGVERETLRVDGQGHLARTPHPPALGSALTNDLITTDYSESLLEFITP 73 + H AL G RG+ERETLRV+ G LA T HP ALGSALT+ ITTD++E+LLEFITP Sbjct: 13 EKHPQALKGIQ-RGLERETLRVNADGTLATTGHPEALGSALTHKWITTDFAEALLEFITP 71 Query: 74 AMPDIADALQRLDEIHRFAYTKLDGEFLWNASMPCVLPPEDEIPLAWYGSSHIGMLKHVY 133 DI L + ++HR+ + E +W SMPC + +I LA YG+S+ G K +Y Sbjct: 72 VDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPCYIAEGQDIELAQYGTSNTGRFKTLY 131 Query: 134 RRGLALRYGRTMQCIAGIHYNFSLSEDLWEVLRDGDDTVDPSLSRRDYQSERYIALIRNF 193 R GL RYG MQ I+G+HYNFSL W+ + GD + ++ S Y +IRN+ Sbjct: 132 REGLKNRYGALMQTISGVHYNFSLPMAFWQA-KCGDIS---GADAKEKISAGYFRVIRNY 187 Query: 194 RRYSWLLMYLFGASPAVATNFLRGRPS 220 R+ W++ YLFGASPA+ ++FL+G+P+ Sbjct: 188 YRFGWVIPYLFGASPAICSSFLQGKPT 214