BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0102.p (307 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5203 [H] COG2918 Gamma-glutamylcysteine synthetase Pae Pseudom... 348 1e-95 VC0556 [H] COG2918 Gamma-glutamylcysteine synthetase Vch Vibrio ... 206 6e-53 gshA [H] COG2918 Gamma-glutamylcysteine synthetase Eco Escherich... 194 2e-49 ZgshA [H] COG2918 Gamma-glutamylcysteine synthetase EcZ Escheric... 193 3e-49 ECs3550 [H] COG2918 Gamma-glutamylcysteine synthetase Ecs Escher... 193 3e-49 >PA5203 [H] COG2918 Gamma-glutamylcysteine synthetase Pae Pseudomonas aeruginosa Length = 527 Score = 348 bits (893), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 186/306 (60%), Positives = 226/306 (73%), Gaps = 1/306 (0%) Query: 1 LSEDTLFLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTK 60 L E TL+LPY TSLRMSDLGYQN+AQA +TP YN+L +YI SL AVS PYPPYE +GTK Sbjct: 222 LDEHTLYLPYATSLRMSDLGYQNNAQAGLTPCYNDLQSYIDSLRQAVSTPYPPYEKVGTK 281 Query: 61 RDGEWVQINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPL 120 +DGEWVQ+NTNILQIENE+YSSIRPKRV +GERPVQAL+ARGVQYVEVRC+DI+PF PL Sbjct: 282 QDGEWVQLNTNILQIENEYYSSIRPKRVTYTGERPVQALAARGVQYVEVRCLDINPFLPL 341 Query: 121 GIGLEAARFLDVFLHFLALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGESLLL 180 GI L+ ARFLD FL F A S L + E + NFLA VKEGRRPGL+LQ +G+ + L Sbjct: 342 GIDLDEARFLDAFLLFCAFSDSPLLNG-ECSDATDNFLAVVKEGRRPGLQLQRRGQPVEL 400 Query: 181 TDWGQQLLDRMEPVADLLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSF 240 W +LL+R+ A LLDR GG H +LA QRAK+AD + TPSA+VL+ +RE G SF Sbjct: 401 QVWANELLERIADTAALLDRARGGEAHAAALAAQRAKVADAELTPSAQVLKVMRERGESF 460 Query: 241 PAYTLANSQRQAAELIARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRAS 300 A++L S+ A PL++ + A F +A SL Q ++ER Q GDFDTFVAAY+AS Sbjct: 461 EAFSLRQSREHAEYFRQHPLAAEEQARFEKMASDSLAEQTELERDQDGDFDTFVAAYQAS 520 Query: 301 TLGNIA 306 LG I+ Sbjct: 521 ILGLIS 526 >VC0556 [H] COG2918 Gamma-glutamylcysteine synthetase Vch Vibrio cholerae Length = 524 Score = 206 bits (524), Expect = 6e-53, Method: Compositional matrix adjust. Identities = 115/297 (38%), Positives = 174/297 (58%), Gaps = 3/297 (1%) Query: 5 TLFLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGE 64 TL+LPY TSLR+SDLGY N AQ+ + +N++ Y++ L A+ P + +G K +GE Sbjct: 223 TLYLPYATSLRLSDLGYTNSAQSVLQIGFNSIEQYLQGLNEAIRTPSAEFAKLGVKVEGE 282 Query: 65 WVQINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGL 124 + Q+N+N+LQIENE Y+ IRPKRV SGERP QAL+ GV+Y+EVR +D++PF+P+GI Sbjct: 283 YRQLNSNVLQIENELYAPIRPKRVTQSGERPSQALARAGVEYIEVRSLDVNPFSPIGINE 342 Query: 125 EAARFLDVFLHFLALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQF--KGESLLLTD 182 + RFLD+FL + AL S E R N+ V EGR+PGL+LQ GE L L Sbjct: 343 DQVRFLDLFLAWTALSDSDPMDGCELACWRDNWRKVVLEGRKPGLELQIGCHGEVLTLQA 402 Query: 183 WGQQLLDRMEPVADLLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPA 242 W +++ + +A+ +D LGG ++ A + +P+ T SA++L EI++ G Sbjct: 403 WAKRVFADLRVIAETMDAALGGDAYQAVCAKLETWIENPELTLSAQILAEIKQHGGLGKT 462 Query: 243 YTLANSQRQAAELIARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFVAAYRA 299 ++ +A L + S+ + + S AQ DIE + +FD ++ Y A Sbjct: 463 GCALGNEYRAVNLQHQYQFYSQD-EMEQEVRRSTQAQADIEAKETLNFDAYLTQYFA 518 >gshA [H] COG2918 Gamma-glutamylcysteine synthetase Eco Escherichia coli K12 Length = 518 Score = 194 bits (493), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 112/290 (38%), Positives = 162/290 (55%), Gaps = 4/290 (1%) Query: 7 FLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWV 66 +LPY TSLR+SDLGY N +Q+N+ +N+L Y+ L A+ P Y IG ++DG+ + Sbjct: 227 YLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRL 286 Query: 67 QINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEA 126 QIN+N+LQIENE Y+ IRPKRV SGE P AL G++Y+EVR +DI+PF+P+G+ + Sbjct: 287 QINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQ 346 Query: 127 ARFLDVFLHFLALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGES--LLLTDWG 184 RFLD+F+ + AL + S E R N+ + EGR+PGL L E+ L G Sbjct: 347 VRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVG 406 Query: 185 QQLLDRMEPVADLLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYT 244 + L ++ VA LD GG ++ A +PD T SAR+LR + + G Sbjct: 407 KDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKA 466 Query: 245 LANSQRQAAELIARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFV 294 A + R L PL + DFVA +AS Q+++E F ++ Sbjct: 467 FAEAYRNL--LREEPLEILREEDFVAEREASERRQQEMEAADTEPFAVWL 514 >ZgshA [H] COG2918 Gamma-glutamylcysteine synthetase EcZ Escherichia coli O157:H7 EDL933 Length = 518 Score = 193 bits (491), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 112/290 (38%), Positives = 161/290 (55%), Gaps = 4/290 (1%) Query: 7 FLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWV 66 +LPY TSLR+SDLGY N +Q+N+ +N+L Y+ L A+ P Y IG +DG+ + Sbjct: 227 YLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIDKDGKRL 286 Query: 67 QINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEA 126 QIN+N+LQIENE Y+ IRPKRV SGE P AL G++Y+EVR +DI+PF+P+G+ + Sbjct: 287 QINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQ 346 Query: 127 ARFLDVFLHFLALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGES--LLLTDWG 184 RFLD+F+ + AL + S E R N+ + EGR+PGL L E+ L G Sbjct: 347 VRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVG 406 Query: 185 QQLLDRMEPVADLLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYT 244 + L ++ VA LD GG ++ A +PD T SAR+LR + + G Sbjct: 407 KDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKA 466 Query: 245 LANSQRQAAELIARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFV 294 A + R L PL + DFVA +AS Q+++E F ++ Sbjct: 467 FAEAYRNL--LREEPLEILREEDFVAEREASERRQQEMEAADTEPFAVWL 514 >ECs3550 [H] COG2918 Gamma-glutamylcysteine synthetase Ecs Escherichia coli O157:H7 Length = 518 Score = 193 bits (491), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 112/290 (38%), Positives = 161/290 (55%), Gaps = 4/290 (1%) Query: 7 FLPYGTSLRMSDLGYQNHAQANITPHYNNLPAYIRSLAHAVSEPYPPYEAIGTKRDGEWV 66 +LPY TSLR+SDLGY N +Q+N+ +N+L Y+ L A+ P Y IG +DG+ + Sbjct: 227 YLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIDKDGKRL 286 Query: 67 QINTNILQIENEFYSSIRPKRVINSGERPVQALSARGVQYVEVRCMDIDPFNPLGIGLEA 126 QIN+N+LQIENE Y+ IRPKRV SGE P AL G++Y+EVR +DI+PF+P+G+ + Sbjct: 287 QINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQ 346 Query: 127 ARFLDVFLHFLALESSCLTSDEEGVENRANFLATVKEGRRPGLKLQFKGES--LLLTDWG 184 RFLD+F+ + AL + S E R N+ + EGR+PGL L E+ L G Sbjct: 347 VRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVG 406 Query: 185 QQLLDRMEPVADLLDRHLGGTQHRDSLAVQRAKLADPDSTPSARVLREIREAGNSFPAYT 244 + L ++ VA LD GG ++ A +PD T SAR+LR + + G Sbjct: 407 KDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKA 466 Query: 245 LANSQRQAAELIARPLSSSKTADFVAIAQASLDAQRDIERTQVGDFDTFV 294 A + R L PL + DFVA +AS Q+++E F ++ Sbjct: 467 FAEAYRNL--LREEPLEILREEDFVAEREASERRQQEMEAADTEPFAVWL 514