BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0053.p (249 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl595 [IQR] COG1028 Dehydrogenases with different specificities... 232 5e-61 L77482 [IQR] COG1028 Dehydrogenases with different specificities... 133 3e-31 mlr1899 [IQR] COG1028 Dehydrogenases with different specificitie... 125 6e-29 CAC3335 [IQR] COG1028 Dehydrogenases with different specificitie... 125 6e-29 L172964 [IQR] COG1028 Dehydrogenases with different specificitie... 120 2e-27 >AGl595 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 249 Score = 232 bits (592), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 133/241 (55%), Positives = 157/241 (65%), Gaps = 1/241 (0%) Query: 2 KTVLITGCSSGFGLEIASYFLQRDWQVIATMRTPRS-DLLPDSPRLRILPLDVTSGTSIA 60 T+LITG SSG+G IA FL R W VIATMR P + D+ RLR+LPLDVT SIA Sbjct: 3 NTILITGTSSGYGKAIAELFLARGWNVIATMRRPSAARFAGDNGRLRVLPLDVTEEASIA 62 Query: 61 AAIAEAGPIDVLVNNAGIGALAAVEAQPMAQVREIFDTNTLGSIAMTQAVIPQMRKRGAG 120 AAI AGPIDVLVNNAGIG + A EA PM+ +R IFDTN+ G +AMTQAVIPQMRKR +G Sbjct: 63 AAIEAAGPIDVLVNNAGIGMVGAFEATPMSAIRTIFDTNSFGVMAMTQAVIPQMRKRQSG 122 Query: 121 VIVNVTSSVTLRSLPLLAVYTASKAAVNAFTESVALELASVGVQARLVLPGRAPQTRFAE 180 I+NVTS+VTL S PL A YTASK A+ FT S+A ELA + +LV PG AP T F Sbjct: 123 TIINVTSTVTLASFPLAAAYTASKQAIEGFTGSLAHELAHFNIGVKLVEPGYAPTTGFTN 182 Query: 181 NARPRMEGAIPDAYGALAAEVFSNFQDHSQITHASDVAAAVWRAATEAAAPMRIAAGEDA 240 NA +E + D Y A A V F + T +DVA AVW A + + + AG DA Sbjct: 183 NAILPLEQLLHDDYAAFARPVLEAFATPALTTRETDVAEAVWSAVHDTSGQLHFPAGADA 242 Query: 241 V 241 V Sbjct: 243 V 243 >L77482 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Lla Lactococcus lactis Length = 277 Score = 133 bits (335), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 91/244 (37%), Positives = 131/244 (53%), Gaps = 19/244 (7%) Query: 2 KTVLITGCSSGFGLEIASYFLQRDWQVIATMRTPRSDLLPD-SPRLRILPLDVTSGTS-- 58 K V ITG S+G G E A F +R W+V A R R + +P ++ L LDVTS S Sbjct: 4 KIVAITGASNGMGFEAAELFAKRGWKVYAGAR--RVEKIPQYENNIKALKLDVTSSESNQ 61 Query: 59 --IAAAIAEAGPIDVLVNNAGIGALAAVEAQPMAQVREIFDTNTLGSIAMTQAVIPQMRK 116 + + EAG IDVL+NNAG G E PM ++R F+TN G++ +TQ V+P MR Sbjct: 62 AFVKKILDEAGHIDVLINNAGYGEYGPAEEIPMDKIRNQFETNFFGAVELTQLVLPTMRA 121 Query: 117 RGAGVIVNVTSSVTLRSLPLLAVYTASKAAVNAFTESVALELASVGVQARLVLPGRAPQT 176 + G IVN++S +PL A Y A+KAA+ +++ + LE+A G+++ V PG Q+ Sbjct: 122 QNYGRIVNISSIGGDVYMPLGAYYHATKAALQQWSDVLDLEVAQFGIRSVCVQPG-GTQS 180 Query: 177 RFA----ENARPRMEGAIPDAYGALAAEVFSN---FQDHSQITHASDVAAAVWRAATEAA 229 + EN++ M AY L V S F +S ASD+A ++AATE Sbjct: 181 SWGNIALENSKKNMSS--NSAYQPLVNSVASGLDRFMKNS--AKASDLAEVFYKAATEIK 236 Query: 230 APMR 233 +R Sbjct: 237 PSLR 240 >mlr1899 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 278 Score = 125 bits (315), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 93/259 (35%), Positives = 132/259 (50%), Gaps = 18/259 (6%) Query: 2 KTVLITGCSSGFGLEIASYFLQRDWQVIATMRTPRSDLLPD-----SPRLRILPLDVTSG 56 KT ITG SSGFG ++ L+R +V AT+R P D L D RL + LDVT Sbjct: 3 KTWFITGASSGFGRQLTELLLERGDRVAATVRKP--DALHDLATKYGERLWVGILDVTDS 60 Query: 57 TSI----AAAIAEAGPIDVLVNNAGIGALAAVEAQPMAQVREIFDTNTLGSIAMTQAVIP 112 +++ A AE IDV+V+NAG A E AQ+ DTN +GSI++ +AVIP Sbjct: 61 SAVRDIVGKAFAELKRIDVVVSNAGYALFGAAEEVADAQIERQIDTNLVGSISVARAVIP 120 Query: 113 QMRKRGAGVIVNVTSSVTLRSLPLLAVYTASKAAVNAFTESVALELASVGVQARLVLPGR 172 +R++G G IV ++SSV + P + VY A+K + F E E+A G++ LV PG Sbjct: 121 DLRQQGGGRIVQISSSVGQSAYPTMGVYAATKWGIEGFYEGTIPEIAPFGIEVTLVEPG- 179 Query: 173 APQTRFAENARPRMEGAIPDAYGALAAEVFSNFQDHSQIT----HASDVAAAVWRAATEA 228 A +T FA ++ G I D Y F + + VA+A+ +A + Sbjct: 180 ASRTNFASSSAD--AGQILDVYDQTPVGDFRRLVASAGLAMFPGDPRKVASAIIASAEQT 237 Query: 229 AAPMRIAAGEDAVEWMRTA 247 AP R+ G DA + TA Sbjct: 238 PAPRRLTLGSDAYALVHTA 256 >CAC3335 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cac Clostridium acetobutylicum Length = 266 Score = 125 bits (315), Expect = 6e-29, Method: Compositional matrix adjust. Identities = 74/187 (39%), Positives = 103/187 (55%), Gaps = 9/187 (4%) Query: 4 VLITGCSSGFGLEIASYFLQRDWQVIATMRTPRSDLLPDSPRLRILPLDVTSGTSIAAAI 63 VLITGCS+G G E+ + + V+AT R + + L D P LPLDVT S+ AI Sbjct: 7 VLITGCSTGIGRELCKILYNKGYTVVATAR--KVEDLKDLPAALKLPLDVTKEESVHKAI 64 Query: 64 AEA----GPIDVLVNNAGIGALAAVEAQPMAQVREIFDTNTLGSIAMTQAVIPQMRKRGA 119 E ID+LVNNAG A+E ++ V+ +F+ N G I M QAVIP+MRKR Sbjct: 65 KEVMLNFHKIDILVNNAGYSVRGALEEVNVSDVKSMFNVNVFGIINMLQAVIPEMRKRKT 124 Query: 120 GVIVNVTSSVTLRSLPLLAVYTASKAAVNAFTESVALELASVGVQARLVLPGRAPQTRF- 178 G I+N+ S P+ Y A+K AV A ++++ LEL+S +Q ++ PG F Sbjct: 125 GKIINIGSISGKFVQPINGAYCAAKFAVEALSDALRLELSSYNIQTTVIEPGPIKTNFFK 184 Query: 179 --AENAR 183 AEN+ Sbjct: 185 TLAENSN 191 >L172964 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Lla Lactococcus lactis Length = 281 Score = 120 bits (302), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 80/247 (32%), Positives = 126/247 (51%), Gaps = 14/247 (5%) Query: 5 LITGCSSGFGLEIASYFLQRDWQVIATMRTPRS-DLLPDSPRLRILPLDVTSGTSIAAAI 63 L+TG S+GFG + + +D++V+AT R + LPD + + LDVT G SI A+ Sbjct: 8 LVTGSSTGFGRALVEELIAQDYKVVATARQLSTLSELPDKENILKISLDVTKGDSIQKAV 67 Query: 64 AEA----GPIDVLVNNAGIGALAAVEAQPMAQVREIFDTNTLGSIAMTQAVIPQMRKRGA 119 AE G IDVLVNNAG G +E + VR+I DTN G+ MT AV+P MRK+ + Sbjct: 68 AETIAKFGRIDVLVNNAGFGYFGVMEESDQSAVRKIMDTNFWGANDMTLAVLPYMRKQKS 127 Query: 120 GVIVNVTSSVTLRSLPLLAVYTASKAAVNAFTESVALELASVGVQARLVLPGRAPQTRFA 179 G I+N S L + L+ Y A+K A+ +++ E+ +G+ + PG F Sbjct: 128 GHILNTVSRGGLTTTENLSYYHATKFAMEGLFQTLRKEVEPLGIFVTNIEPG-----SFR 182 Query: 180 ENARPRMEGAIPDAYGAL--AAEVFSNFQDHS--QITHASDVAAAVWRAATEAAAPMRIA 235 + R + + PD + A ++ + +S Q+ + + A A + + PM Sbjct: 183 TDWAGRSKESAPDKFSDYEKAHKLLEHLNSYSGTQVGNPASAAKAFIKVSKLENPPMHYL 242 Query: 236 AGEDAVE 242 G+DA + Sbjct: 243 MGKDAYQ 249