BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0040.p (286 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl889 [E] COG1176 ABC-type spermidine/putrescine transport syst... 277 2e-74 SMb21274 [E] COG1176 ABC-type spermidine/putrescine transport sy... 266 4e-71 SMa0952 [E] COG1176 ABC-type spermidine/putrescine transport sys... 148 1e-35 mll6253_1 [E] COG1176 ABC-type spermidine/putrescine transport s... 147 2e-35 mlr6080_1 [E] COG1176 ABC-type spermidine/putrescine transport s... 147 4e-35 >AGl889 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 326 Score = 277 bits (709), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 146/261 (55%), Positives = 195/261 (74%), Gaps = 9/261 (3%) Query: 24 CFLLPVAALLLDAFRIGGPDSLDGGPLQWSLARFADFFAEPLHLQVLWRTLRIAALVTLC 83 FL+PV LL + FR + DG W+L+ +A FF++PL+ V RTL++ ALVT+ Sbjct: 73 VFLVPVFMLLSEGFR-----TTDG----WTLSAYAAFFSDPLNQTVFLRTLKLGALVTVV 123 Query: 84 SALLCYPAAQAIVRVHARWRGLVLGMMILPLMVSPIARTYAWIVLFGRNGAVNAVLLHLG 143 SA++ Y AA AI+ + +G V+ +++LPLM+SP+ARTYAWIV+ GR G VN L +G Sbjct: 124 SAVVGYAAAFAIINLSPGKKGHVVNLVVLPLMISPVARTYAWIVILGRTGIVNQALQAVG 183 Query: 144 LADEPQRLLFTEMAVFVGLLQLLLPLMLMSLVSAMENISADLALAASTLGANAWQVFTRV 203 L+D P R+LF+E AVF+GLLQL LPLM++SL+SA+EN+ D AA LGAN +QVF +V Sbjct: 184 LSDAPIRILFSETAVFIGLLQLFLPLMIISLISALENMPKDTIAAARVLGANWFQVFWKV 243 Query: 204 TLPLTQEGLIVGGTLVFTGSVTAYVTPALLGGPKVLMLETLLYQKVSVESDFGTANVIAV 263 LPLT+EGL+VGGTLVFTGS+TAY+TPA+LGG KVLMLETLLYQ+V+V ++F A+VIA Sbjct: 244 ILPLTKEGLVVGGTLVFTGSLTAYITPAILGGSKVLMLETLLYQQVTVSNNFVAASVIAF 303 Query: 264 ILVAMTLAVNAVLKRIAAPRR 284 IL+ M+ A N +LKRIA R Sbjct: 304 ILIVMSFAANILLKRIATARN 324 >SMb21274 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Sme Sinorhizobium meliloti Length = 299 Score = 266 bits (680), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 143/256 (55%), Positives = 192/256 (75%), Gaps = 9/256 (3%) Query: 28 PVAALLLDAFRIGGPDSLDGGPLQWSLARFADFFAEPLHLQVLWRTLRIAALVTLCSALL 87 PV LL + FR + DG WS++ ++ F ++PL+ V RTLR+ A VT+ +A++ Sbjct: 50 PVVILLAEGFR-----NADG----WSVSAYSGFLSDPLNRTVFLRTLRLGAAVTVVAAVI 100 Query: 88 CYPAAQAIVRVHARWRGLVLGMMILPLMVSPIARTYAWIVLFGRNGAVNAVLLHLGLADE 147 Y AA AIV + + +G ++G+++LPLM+SP+ARTYAWIV+ GR G VN L +GL DE Sbjct: 101 GYAAAFAIVNLPPKGKGRMIGLVVLPLMISPVARTYAWIVILGRTGIVNQALTSVGLTDE 160 Query: 148 PQRLLFTEMAVFVGLLQLLLPLMLMSLVSAMENISADLALAASTLGANAWQVFTRVTLPL 207 P R LFTE AVFVGLLQL LPLM+++L+SA+EN+ D AA LGAN +QVF +V LPL Sbjct: 161 PVRFLFTETAVFVGLLQLFLPLMILALISALENMPKDAIPAARVLGANWFQVFWKVILPL 220 Query: 208 TQEGLIVGGTLVFTGSVTAYVTPALLGGPKVLMLETLLYQKVSVESDFGTANVIAVILVA 267 T+EGL++GGTLVFTGS+TAY+TPA+LGG KVLMLETLLYQ+V+V +DF +A+VIA IL+ Sbjct: 221 TREGLVIGGTLVFTGSLTAYITPAILGGSKVLMLETLLYQRVTVANDFVSASVIAFILIV 280 Query: 268 MTLAVNAVLKRIAAPR 283 M+ A N +LKR+A R Sbjct: 281 MSFAANLLLKRLATAR 296 >SMa0952 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Sme Sinorhizobium meliloti Length = 296 Score = 148 bits (374), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 94/269 (34%), Positives = 153/269 (56%), Gaps = 18/269 (6%) Query: 13 LVTPALIFTSVCFLLPVAALLLDAFRIGGPDSLDGGPLQWSLARFADFFAEPLHLQVLWR 72 LVTP L FL PVA L+ +F GG + + + F EPLHL+VL+ Sbjct: 31 LVTPLLAAMLAGFLYPVARLIALSF--------SGG----TFSHYRRIFTEPLHLEVLFS 78 Query: 73 TLRIAALVTLCSALLCYPAAQAIVRVHARWRGLVLGM---MILPLMVSPIARTYAWIVLF 129 T+ +A +VT+ LL +P A + R+ RGL + + + +PL S + R+YAW+VL Sbjct: 79 TIEVAFVVTVAGLLLGFPVAYLMARLS---RGLAMAVAACVFVPLWTSVLIRSYAWVVLL 135 Query: 130 GRNGAVNAVLLHLGLADEPQRLLFTEMAVFVGLLQLLLPLMLMSLVSAMENISADLALAA 189 RNG VN +L G+ + P +L++T+ AV + + +L+P M++ + SA+ + D AA Sbjct: 136 QRNGIVNKLLADTGVTEGPLKLIYTQGAVILAMTHVLMPFMILPIYSALRALPPDYVRAA 195 Query: 190 STLGANAWQVFTRVTLPLTQEGLIVGGTLVFTGSVTAYVTPALLGGPKVLMLETLLYQKV 249 LGA + F VTLPL+ G+ G + F ++ Y+TPAL+GGP +++ TL+ Q+ Sbjct: 196 RNLGAGPIRAFVTVTLPLSLPGIFAGSVMCFVLALGFYITPALVGGPSSMLMATLIGQQT 255 Query: 250 SVESDFGTANVIAVILVAMTLAVNAVLKR 278 +V D+ A ++ +L+A+TL + +R Sbjct: 256 TVLLDWPFAAALSTVLLAVTLLFVLMFRR 284 >mll6253_1 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Mlo Mesorhizobium loti Length = 340 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 96/283 (33%), Positives = 148/283 (52%), Gaps = 10/283 (3%) Query: 2 LASSKSRLGLMLVTPALIFTSVCFLLPVAALLLDAFRIGGPDSLDGGPLQWSLARFADFF 61 LA + SR +L+ PA + VCF +P+A L+ +F P +L + +F Sbjct: 15 LAPNTSREAFVLLAPATVILGVCFFIPLAGLVALSFLSPEP----------TLMHYKEFL 64 Query: 62 AEPLHLQVLWRTLRIAALVTLCSALLCYPAAQAIVRVHARWRGLVLGMMILPLMVSPIAR 121 +L RT +A VT LL YP A IV A GL++ ++LP S + R Sbjct: 65 GSWTSWVILGRTFSLALSVTSICLLLGYPLAYWIVSARAWVSGLLVFAVVLPFFTSYLVR 124 Query: 122 TYAWIVLFGRNGAVNAVLLHLGLADEPQRLLFTEMAVFVGLLQLLLPLMLMSLVSAMENI 181 +YAW+VL GR G +N L+ +G + P L++ + +V V ++ +LLP +M L S M I Sbjct: 125 SYAWMVLLGRFGIINQFLMKVGFTERPLDLIYNQFSVDVAMVYMLLPFTVMVLSSVMRGI 184 Query: 182 SADLALAASTLGANAWQVFTRVTLPLTQEGLIVGGTLVFTGSVTAYVTPALLGGPKVLML 241 S L AAS+LGA Q F R+ PL+ G++ +VF ++ Y+TPALLGGP+ + L Sbjct: 185 SPSLTRAASSLGAAPGQNFWRIFFPLSMPGVVASSLIVFVFALGFYITPALLGGPRNVTL 244 Query: 242 ETLLYQKVSVESDFGTANVIAVILVAMTLAVNAVLKRIAAPRR 284 L+ V+ ++ A +A +L+ +TL + V R R Sbjct: 245 PMLIDTYVNGTLNWPLAAAVATVLLLITLFLYTVSNRFVGTER 287 >mlr6080_1 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Mlo Mesorhizobium loti Length = 315 Score = 147 bits (370), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 90/265 (33%), Positives = 146/265 (55%), Gaps = 10/265 (3%) Query: 8 RLGLMLVTPALIFTSVCFLLPVAALLLDAFRIGGPDSLDGGPLQWSLARFADFFAEPLHL 67 R+ + L PAL V F +P+ L L + + PD S+ + FF ++ Sbjct: 9 RMPIALTVPALAILLVVFGVPIVQLFLTS--LNAPD--------LSMVNYRAFFGRSANV 58 Query: 68 QVLWRTLRIAALVTLCSALLCYPAAQAIVRVHARWRGLVLGMMILPLMVSPIARTYAWIV 127 +VL++T+ I+ + T ++ YPAA IV R R ++ ++++P + S +ARTYAWI+ Sbjct: 59 RVLYQTIEISLVATAICLIIGYPAAYLIVTASKRLRMTLIVLVVIPYLTSGLARTYAWII 118 Query: 128 LFGRNGAVNAVLLHLGLADEPQRLLFTEMAVFVGLLQLLLPLMLMSLVSAMENISADLAL 187 + G G +N +LL LGL P L++ MAV++ ++ ++LP+M++ LVS M I L Sbjct: 119 ILGDGGLINNLLLELGLISSPLPLIYNRMAVYIAMVHIMLPMMILPLVSVMMGIDRSLMA 178 Query: 188 AASTLGANAWQVFTRVTLPLTQEGLIVGGTLVFTGSVTAYVTPALLGGPKVLMLETLLYQ 247 AA ++GA + F RV PL+ G+ G LVF + Y+TPA +GG + ML T + Sbjct: 179 AARSMGAGPFTAFWRVFFPLSLPGVRSGSLLVFVICLGFYITPAAVGGLRDAMLSTFIAS 238 Query: 248 KVSVESDFGTANVIAVILVAMTLAV 272 +VS D + IL+A+ +AV Sbjct: 239 QVSTGFDMAPIAASSFILLAIAVAV 263