BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0096.p (252 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0935 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Rso Rals... 347 2e-95 PM1297 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pmu Paste... 261 1e-69 PA3743 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pae Pseud... 260 2e-69 HI0202 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Hin Haemo... 259 4e-69 VC0563 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Vch Vibri... 254 9e-68 >RSc0935 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Rso Ralstonia solanacearum Length = 262 Score = 347 bits (889), Expect = 2e-95, Method: Compositional matrix adjust. Identities = 171/249 (68%), Positives = 201/249 (80%), Gaps = 3/249 (1%) Query: 2 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 61 MQFDV++LFPEMF ALT GIT RA +QQ + L+ WNPR+FTTDN+RTVDDRPYGGGPGM Sbjct: 1 MQFDVISLFPEMFRALTDWGITSRAAKQQVYTLRTWNPRDFTTDNYRTVDDRPYGGGPGM 60 Query: 62 VMLAKPLQAAIEAAQQRQQALGLARPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 121 VMLAKPL+AA++A ++ Q A V+ LSPQG PLTH RV+ L+Q LLCGRYE Sbjct: 61 VMLAKPLEAALDAIREAQAP---APSHVVLLSPQGRPLTHRRVMELAQLPALTLLCGRYE 117 Query: 122 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 181 AIDQRL+D+ VDEEIS+GDFVLSGGE+ AMA++DAV+R LPG L D+ SAV+DSFVNGLL Sbjct: 118 AIDQRLVDRRVDEEISLGDFVLSGGEIAAMAIIDAVVRQLPGVLGDAQSAVQDSFVNGLL 177 Query: 182 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQSKRPDLIVRAREQGLLSKA 241 D PHYTRPE YEG VP +LLGGHHAEI+KWRRQ+AL T KRPDLI AR QGLLS++ Sbjct: 178 DCPHYTRPEEYEGVRVPDVLLGGHHAEIEKWRRQQALLNTMRKRPDLIEAARRQGLLSRS 237 Query: 242 DEKFLGSLA 250 DE FL + A Sbjct: 238 DEVFLAAAA 246 >PM1297 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pmu Pasteurella multocida Length = 245 Score = 261 bits (666), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 126/228 (55%), Positives = 167/228 (73%), Gaps = 4/228 (1%) Query: 2 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 61 M +++LFPEMF A+T+ G+T RA++Q ++ WNPR+FT D H+TVDDRPYGGGPGM Sbjct: 1 MLVGIISLFPEMFKAITEFGVTGRAVKQNLLQVRCWNPRDFTHDKHKTVDDRPYGGGPGM 60 Query: 62 VMLAKPLQAAIEAAQQRQQALGLARPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 121 +M+ +PL+ AI+AA+ +G +VIYLSPQG L V L+Q +LLCGRYE Sbjct: 61 LMMVQPLRDAIQAAKAE---VGEG-AKVIYLSPQGRKLDQAGVKELAQHQKLILLCGRYE 116 Query: 122 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 181 ID+RL++ VDEE S+GD+VL+GGELPAM L+DAV R +PG L ASA EDSF GLL Sbjct: 117 GIDERLIETEVDEEWSVGDYVLTGGELPAMTLIDAVARFIPGVLGKQASADEDSFAEGLL 176 Query: 182 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQSKRPDLI 229 D PHYTRPEV +G VP +L+ G+H EI+KWR +++LE T +RP+L+ Sbjct: 177 DCPHYTRPEVLDGLAVPPVLMSGNHEEIRKWRLRQSLERTWLRRPELL 224 >PA3743 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Pae Pseudomonas aeruginosa Length = 252 Score = 260 bits (665), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 132/239 (55%), Positives = 171/239 (71%), Gaps = 7/239 (2%) Query: 6 VVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGMVMLA 65 VV++FPEMF A++ GIT RA++Q L WNPR++T D H+TVDDRP+GGGPGMVM Sbjct: 9 VVSIFPEMFRAISDYGITSRAVKQGLLTLTCWNPRDYTEDRHQTVDDRPFGGGPGMVMKI 68 Query: 66 KPLQAAIEAAQQRQQALGLARPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYEAIDQ 125 KPL+ A+ A+Q A G + +VIYLSPQG LT V L+++ +L+ GRYE ID+ Sbjct: 69 KPLEGALADARQ---AAGGRKAKVIYLSPQGRQLTQAGVRELAEEEALILIAGRYEGIDE 125 Query: 126 RLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLLDSPH 185 R +++ VDEE SIGD+VLSGGELPAM L+DAV RLLPGAL + SA EDSF +GLLD PH Sbjct: 126 RFIEEHVDEEWSIGDYVLSGGELPAMVLVDAVTRLLPGALGHADSAEEDSFTDGLLDCPH 185 Query: 186 YTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQSKRPDLI----VRAREQGLLSK 240 YTRPEVY + VP +LL G+H I++WR Q+AL T +R DL+ + EQ LL++ Sbjct: 186 YTRPEVYADKRVPEVLLSGNHEHIRRWRLQQALGRTWERRADLLDSRSLSGEEQKLLAE 244 >HI0202 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Hin Haemophilus influenzae Length = 246 Score = 259 bits (662), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 124/228 (54%), Positives = 163/228 (71%), Gaps = 4/228 (1%) Query: 2 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 61 M V++LFPEMF A+T+ G+T RA++ ++ WNPR+FT D H+TVDDRPYGGGPGM Sbjct: 1 MWIGVISLFPEMFKAITEFGVTGRAVKHNLLKVECWNPRDFTFDKHKTVDDRPYGGGPGM 60 Query: 62 VMLAKPLQAAIEAAQQRQQALGLARPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 121 +M+ +PL+ AI A+ +VIYLSPQG L V L+Q+ +L+CGRYE Sbjct: 61 LMMVQPLRDAIHTAKAAAGE----GAKVIYLSPQGRKLDQGGVTELAQNQKLILVCGRYE 116 Query: 122 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 181 ID+RL+ +DEE SIGD+VL+GGELPAM L+DAV R +PG L ASA EDSF +GLL Sbjct: 117 GIDERLIQTEIDEEWSIGDYVLTGGELPAMTLIDAVARFIPGVLGKQASAEEDSFADGLL 176 Query: 182 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQSKRPDLI 229 D PHYTRPEV EG VP +L+ GHH EI+KWR +++L+ T +RP+L+ Sbjct: 177 DCPHYTRPEVLEGLTVPPVLMSGHHEEIRKWRLKQSLQRTWLRRPELL 224 >VC0563 [J] COG0336 tRNA-(guanine-N1)-methyltransferase Vch Vibrio cholerae Length = 247 Score = 254 bits (650), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 118/228 (51%), Positives = 167/228 (73%), Gaps = 4/228 (1%) Query: 2 MQFDVVTLFPEMFAALTQSGITRRALEQQRWGLQLWNPREFTTDNHRTVDDRPYGGGPGM 61 M +V+LFPEMF ++T G+T +A+++ ++ WNPR+F D RTVDD+PYGGGPGM Sbjct: 1 MWVGIVSLFPEMFRSVTDFGVTGQAVKKGLLSVEAWNPRDFAHDKRRTVDDKPYGGGPGM 60 Query: 62 VMLAKPLQAAIEAAQQRQQALGLARPRVIYLSPQGAPLTHERVVRLSQDSGAVLLCGRYE 121 +M+ +PL+ AI AA+Q + +VIYLSPQG L + V L+Q+ +L+CGRYE Sbjct: 61 LMMVQPLRDAIHAAKQASPG----KTKVIYLSPQGRKLDQQGVEELAQNQNLILICGRYE 116 Query: 122 AIDQRLLDKCVDEEISIGDFVLSGGELPAMALMDAVIRLLPGALNDSASAVEDSFVNGLL 181 +D+R+++ VDEE SIGDFV++GGELPAM L+D+V R +PG L D ASA EDSF NGLL Sbjct: 117 GVDERIIESEVDEEWSIGDFVMTGGELPAMTLIDSVSRFIPGVLGDFASAEEDSFANGLL 176 Query: 182 DSPHYTRPEVYEGEPVPSILLGGHHAEIQKWRRQRALEATQSKRPDLI 229 D PHYTRPEV +G+ VP++L G+H +I++WR +++L T +RP+L+ Sbjct: 177 DCPHYTRPEVLDGKEVPAVLKSGNHEDIRRWRLKQSLGRTWLRRPELL 224