BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0041.p (312 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA5422 [G] COG0676 Uncharacterized enzymes related to aldose 1-e... 286 5e-77 XF2270 Non COG 165 1e-40 VC2001 [G] COG0676 Uncharacterized enzymes related to aldose 1-e... 149 1e-35 YPO2156 [G] COG0676 Uncharacterized enzymes related to aldose 1-... 140 3e-33 ZyeaD [G] COG0676 Uncharacterized enzymes related to aldose 1-ep... 126 7e-29 >PA5422 [G] COG0676 Uncharacterized enzymes related to aldose 1-epimerase Pae Pseudomonas aeruginosa Length = 318 Score = 286 bits (732), Expect = 5e-77, Method: Compositional matrix adjust. Identities = 145/296 (48%), Positives = 190/296 (64%), Gaps = 4/296 (1%) Query: 16 IQRIEVNQLQCLRVQTERAELLIAEQGAQVLRYQVHGQEPVIWMSEEAAFEAGNSVRGGI 75 +++++V+ L C R+ T E LIA+QGAQ+L YQ H Q P++W+S+EA + G SVRGG+ Sbjct: 23 VEQVQVDDLACWRIHTAHGEALIAQQGAQLLSYQPHEQPPLVWLSDEAGYRRGQSVRGGV 82 Query: 76 PVCWPWFGDLMRNPPQVQA-LHAGGRLPAHGLVRGIDWLLMENAIEGNTVRLVLAIPPTT 134 PVCWPWFG L RNP +V+ + G + PAHGLVR +DW + + A E + + + Sbjct: 83 PVCWPWFGPLERNPQEVRGQVEDGLKAPAHGLVRAVDWKIDDIAEEDGDLCVSFRLDAPD 142 Query: 135 ESPALRHLPPGIELRLEILLDDRLHLKLTTRNGSEQHIALTQALHSYFAVSSIHNVTVDG 194 P H LR+ +RL L+LT+ N + + L+QALH+YFAVS + ++G Sbjct: 143 GLPGWPH---PAALRMLFRFGERLTLRLTSENLGSKPLVLSQALHTYFAVSDSREIAIEG 199 Query: 195 LEGQRYIETLEGWAEREQKRPLVVSSETDRIYLDLPPHLAIRDEGWNRRIHIEASGSTSA 254 LEG RYIETLE W ER Q + V E DRIYL L L I+D W R IH+ A S SA Sbjct: 200 LEGARYIETLENWEERTQHGAVRVKGELDRIYLGLERDLLIKDPRWERSIHLHALDSRSA 259 Query: 255 VVWNPWVSKARRLSQFADDAWQRMLCIETANVMDDLVELEPGAEHTMGVSLWGETL 310 VVWNPWV K+RRLSQFADDAWQ+MLCIET V DDL+ + PG + GV +W E L Sbjct: 260 VVWNPWVDKSRRLSQFADDAWQQMLCIETGRVWDDLMVVAPGRSESFGVQIWSEPL 315 >XF2270 Non COG Length = 288 Score = 165 bits (417), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 108/303 (35%), Positives = 158/303 (52%), Gaps = 31/303 (10%) Query: 12 DLPHIQRIEVNQLQCLRVQTERAELLIAEQGAQVLRYQVHGQEPVIWMSEEAAFEAGNSV 71 D+P + + + L +QT + I+ G Q+L + G E VIW+S ++ Sbjct: 3 DIPGLSTGDFHGFPSLLIQTPFSHAAISLFGGQLLSFVPSGGEEVIWLSPLVQ-PPPTAI 61 Query: 72 RGGIPVCWPWFGDLMRNPPQVQALHAGGR--LPAHGLVRGIDWLLMENAIEGN-TVRLVL 128 RGGIP+CWP+FG G R +PAHG VR + W +E+ E + ++ LVL Sbjct: 62 RGGIPICWPYFG------------RQGQRDTMPAHGFVRTVPWQQVESCQENDGSLFLVL 109 Query: 129 AIPPTTESPALRHLPPGIELRLEILLDDRLHLKLTTRNGSEQHIALTQALHSYFAVSSIH 188 A PP L+HL G+ LR+ + + L +L T N S I+ TQALH+YF VS+ Sbjct: 110 APPP------LKHL--GLHLRMTLRIGRTLEQELATENISHAPISFTQALHNYFRVSNAL 161 Query: 189 NVTVDGLEGQRYIETLEGWAEREQKRPLVV------SSETDRIYLDLPPHLAIRDEGWNR 242 VTV GL+G Y++ EG+ +R +++ DRIY+D H + D ++R Sbjct: 162 QVTVQGLDGVEYLDKFEGYTQRHRQQGHWTLQDPRDPGRADRIYVDTSGHYVLVDPLFSR 221 Query: 243 RIHIEASGSTSAVVWNPWVSKARRLSQFADDAWQRMLCIETANVMDDLVELEPGAEHTMG 302 RI + GS S VVWNP + A + A D W+ LC+E AN D +EL PGA H + Sbjct: 222 RIVLTTEGSHSLVVWNPGHAGASNMIDVA-DGWRDYLCLEPANAGPDEIELLPGARHVLR 280 Query: 303 VSL 305 S+ Sbjct: 281 QSI 283 >VC2001 [G] COG0676 Uncharacterized enzymes related to aldose 1-epimerase Vch Vibrio cholerae Length = 296 Score = 149 bits (375), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 86/285 (30%), Positives = 142/285 (49%), Gaps = 20/285 (7%) Query: 20 EVNQLQCLRVQTERAELLIAEQGAQVLRYQVHGQEPVIWMSEEAAFEAGNSVRGGIPVCW 79 E +Q++ +RV ++A I+ G VL +Q GQ ++WMS +A ++ ++RGGIPVCW Sbjct: 20 EQDQVKIVRVIHDKASAAISLHGGHVLSFQPKGQADLLWMSSQAIYDGKAALRGGIPVCW 79 Query: 80 PWFGDLMRNPPQVQALHAGGRLPAHGLVRGIDWLLMENAIEGNTVRLVLAIPPTTESPAL 139 PWFG + PAHG R +W L+E+ V + L + T ES A+ Sbjct: 80 PWFGRIAA--------------PAHGFARNQEWKLIEHREIEQGVIVTLGLQATPESLAI 125 Query: 140 RHLPPGIELRLEILLDDRLHLKLTTRNGSEQHIALTQALHSYFAVSSIHNVTVDGLEGQR 199 P ++RL + + + L + L N + + ALH+Y V I G+ G Sbjct: 126 --WPYQFDVRLHVEIRESLTVTLDVTNTDSKAWTFSGALHTYLHVGDIRETQTSGV-GTE 182 Query: 200 YIETLEGWAEREQKRPLVVSSETDRIYLDLPPHLAIRDEGWNRRIHIEASGSTSAVVWNP 259 YI++L+ Q L ++ DR+Y P + ++D R + +E G SAV+WNP Sbjct: 183 YIDSLQNSRLCTQHGLLTLTDTIDRVYTQPEPLITVQDPALARTLTVENQGHNSAVLWNP 242 Query: 260 WVSKARRLSQFADDAWQRMLCIET---ANVMDDLVELEPGAEHTM 301 W A+ ++ D+ + LC+E+ A + + + L+PG H + Sbjct: 243 WQQGAQSMADMNDEGYLHFLCVESTLHAPSLAEGITLQPGQNHQL 287 >YPO2156 [G] COG0676 Uncharacterized enzymes related to aldose 1-epimerase Ype Yersinia pestis Length = 291 Score = 140 bits (354), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 84/278 (30%), Positives = 138/278 (49%), Gaps = 21/278 (7%) Query: 14 PHIQRIEVNQLQCLRVQTERAELLIAEQGAQVLRYQVHGQEPVIWMSEEAAFEAGNSVRG 73 P+I + ++++L + V + I+ QGA +L YQ G++P+IW+S F+ G ++RG Sbjct: 16 PYISQRQLDELPVVVVSHPKVRAAISLQGAHLLAYQPSGEQPIIWLSNNTPFKEGIAIRG 75 Query: 74 GIPVCWPWFGDLMRNPPQVQALHAGGRLPAHGLVRGIDWLLMENAIEGNTVRLVLAIPPT 133 G+P+CWPWFG P Q PAHG R + W L +A + N ++L Sbjct: 76 GVPICWPWFG------PTAQ--------PAHGFARILPWTL--SAHDENENGVILTFTLE 119 Query: 134 TESPALRHLPPGIELRLEILLDDRLHLKLTTRNGSEQHIALTQALHSYFAVSSIHNVTVD 193 ++ P L L D ++L + +G Q A ALH+YF + I + V Sbjct: 120 DNDMTRQYWPHAFTLIARFKLGDECEVELES-HGDYQAAA---ALHTYFQIGDISQIKVS 175 Query: 194 GLEGQRYIETLEGWAEREQKRPLVVSSETDRIYLDLPPHLAIRDEGWNRRIHIEASGSTS 253 GL G++Y++ + A+ Q+ LV + +TDR+Y + I+D R I + + Sbjct: 176 GL-GEKYLDKVLKVADVTQQGDLVFNGQTDRVYTQPEAYSLIKDAALKRTIEVHHHHQSD 234 Query: 254 AVVWNPWVSKARRLSQFADDAWQRMLCIETANVMDDLV 291 V WNP + + +D ++ M+C+ETA V LV Sbjct: 235 VVAWNPGAELSCSMVDMPNDGYKTMVCVETARVNKPLV 272 >ZyeaD [G] COG0676 Uncharacterized enzymes related to aldose 1-epimerase EcZ Escherichia coli O157:H7 EDL933 Length = 301 Score = 126 bits (316), Expect = 7e-29, Method: Compositional matrix adjust. Identities = 82/272 (30%), Positives = 126/272 (46%), Gaps = 19/272 (6%) Query: 14 PHIQRIEVNQLQCLRVQTERAELLIAEQGAQVLRYQVHGQEPVIWMSEEAAFEAGNSVRG 73 P + R ++++L + V + + A QGA +L ++ G+E V+W+S F+ G ++RG Sbjct: 23 PVLSRRKLDELDLIVVDHPQVKASFALQGAHLLSWKPAGEEEVLWLSNNTPFKNGVAIRG 82 Query: 74 GIPVCWPWFGDLMRNPPQVQALHAGGRLPAHGLVRGIDWLLMENAIEGNTVRLVLAIPPT 133 G+PVCWPWFG P Q LPAHG R + W L + + N V L + + Sbjct: 83 GVPVCWPWFG-----PAAQQG------LPAHGFARNLPWTLKSHREDANGVALTFELTQS 131 Query: 134 TESPALRHLPPGIELRLEILLDDRLHLKLTTRNGSEQHIALTQALHSYFAVSSIHNVTVD 193 E+ + L +D + L E T ALH+YF V I V+V Sbjct: 132 EETK--KFWLHDFTLLAHFRVDKNCEIDLEAHGEFET----TSALHTYFNVGDIAKVSVS 185 Query: 194 GLEGQRYIETLEGWAEREQKRPL-VVSSETDRIYLDLPPHLAIRDEGWNRRIHIEASGST 252 GL G R+I+ + E + TDR+YL+ I DE NR I + Sbjct: 186 GL-GDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCSVINDEALNRIIAVGHQHHL 244 Query: 253 SAVVWNPWVSKARRLSQFADDAWQRMLCIETA 284 + V WNP + + + DD ++ +C+ETA Sbjct: 245 NVVGWNPGPALSVSMGDMPDDGYKTFVCVETA 276