BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0032.0022.p (492 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA3504 [C] COG1012 NAD-dependent aldehyde dehydrogenases Pae Pse... 815 0.0 SMa2213 [C] COG1012 NAD-dependent aldehyde dehydrogenases Sme Si... 401 e-111 BH0539 [C] COG1012 NAD-dependent aldehyde dehydrogenases Bha Bac... 384 e-106 YER073w [C] COG1012 NAD-dependent aldehyde dehydrogenases Sce Sa... 383 e-106 BS_dhaS [C] COG1012 NAD-dependent aldehyde dehydrogenases Bsu Ba... 379 e-105 >PA3504 [C] COG1012 NAD-dependent aldehyde dehydrogenases Pae Pseudomonas aeruginosa Length = 494 Score = 815 bits (2106), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/492 (82%), Positives = 446/492 (90%) Query: 1 MLNLNDIQPICIAGQWRPGAGDRYATLYPATGEAVASLHAASVADVEEAILGADRAFRTS 60 M++LN IQPICIAG+WR G GD Y +LYPATGEAVA LHAAS+ DVEEA+ GA RAFR S Sbjct: 1 MIDLNPIQPICIAGEWRAGGGDLYESLYPATGEAVARLHAASLEDVEEAMAGAHRAFRES 60 Query: 61 GWAQKKPHERAAVLFRVAQLIRENGEALAQLQRLDNGKPISETRNLVASAAATFQFFAAA 120 GWAQ+KPHERA VL+R+A LIRE E LAQLQRLDNGKPI ETRNLVASAAATFQFFAAA Sbjct: 61 GWAQRKPHERATVLYRIAALIRERSEELAQLQRLDNGKPIRETRNLVASAAATFQFFAAA 120 Query: 121 CETLEESLTPMRGDNLTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAE 180 CETLEES+TP RGD ++MSV+EPMGVV AITPWNSPIASEAQK+APALAAGNAVVVKPAE Sbjct: 121 CETLEESITPSRGDFVSMSVYEPMGVVVAITPWNSPIASEAQKLAPALAAGNAVVVKPAE 180 Query: 181 VTPLAALALARLCIEAGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAH 240 +TPLAALALAR+C EAG+P GL+SVLPGKG++IGDA+T HPL +RVSFTGGT TGKHIA Sbjct: 181 ITPLAALALARICDEAGLPRGLVSVLPGKGALIGDALTRHPLARRVSFTGGTRTGKHIAR 240 Query: 241 IAADKMMPVSLELGGKSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHE 300 IAADKMMPVSLELGGKSPTMV DAD+DHAVAGVLYGIFSSSGESCIAGSRLFVA ++ Sbjct: 241 IAADKMMPVSLELGGKSPTMVLADADLDHAVAGVLYGIFSSSGESCIAGSRLFVASERYD 300 Query: 301 PFMERLAAGAAALRVGDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGVRPKG 360 FMERLA GAAALRVGDPADERTQMGPLI+ARHRE +ERYV+MGV +GGR+RTGG+RP G Sbjct: 301 EFMERLATGAAALRVGDPADERTQMGPLISARHRESVERYVEMGVAEGGRLRTGGLRPHG 360 Query: 361 PAFERGYFYTPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIW 420 AF+RGYFYTPTIIEG+ N R+C+EE+FGPVLVAMPF EE LI +ANDS YALAAGIW Sbjct: 361 GAFDRGYFYTPTIIEGLTNGARLCREEVFGPVLVAMPFASEEALIEEANDSCYALAAGIW 420 Query: 421 TRDYKRAWRVGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMY 480 TRD++RAWR+GRAVQAG VWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQK++Y Sbjct: 421 TRDFQRAWRLGRAVQAGTVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKSLY 480 Query: 481 WGLNEQPLPWAG 492 WGLNEQPL WAG Sbjct: 481 WGLNEQPLAWAG 492 >SMa2213 [C] COG1012 NAD-dependent aldehyde dehydrogenases Sme Sinorhizobium meliloti Length = 494 Score = 401 bits (1030), Expect = e-111, Method: Compositional matrix adjust. Identities = 206/487 (42%), Positives = 297/487 (60%), Gaps = 2/487 (0%) Query: 1 MLNLNDIQPICIAGQW-RPGAGDRYATLYPATGEAVASLHAASVADVEEAILGADRAFRT 59 M NL + + I GQW P +G+ T+ P T A + + D + A+ A RAF Sbjct: 1 MQNLPKLS-MYIDGQWVAPASGEYIETVDPFTARPWALVPRGNAEDADRAVRAAHRAFSQ 59 Query: 60 SGWAQKKPHERAAVLFRVAQLIRENGEALAQLQRLDNGKPISETRNLVASAAATFQFFAA 119 W + P ER ++ R A LI E+ +ALA ++ DNG+ ++E + + + ++A Sbjct: 60 GPWGKMHPTERGRIIQRFAALIEEHADALADIEVRDNGRLLAEMTHQIRYIPRWYHYYAG 119 Query: 120 ACETLEESLTPMRGDNLTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPA 179 + +E +L P L+ S HEP+GV I PWN+P+ + K APALAAGN +V+KPA Sbjct: 120 FADKIEGTLHPCDKPALSFSRHEPLGVCVGIVPWNAPLLLFSLKAAPALAAGNTLVMKPA 179 Query: 180 EVTPLAALALARLCIEAGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIA 239 E T AL L L +AG P G+I+V+ G G +G+ + HPL + V FTG T TG H+ Sbjct: 180 EFTSATALKLMELVEKAGFPTGVINVVTGYGPEVGEPLVTHPLTRHVGFTGSTKTGAHLY 239 Query: 240 HIAADKMMPVSLELGGKSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLH 299 +AA + VSLELGGKSP +VF DAD+D+AV GV+ GIF + G++CIAGSRL V RS+H Sbjct: 240 SLAAKDVKRVSLELGGKSPNIVFGDADLDNAVRGVVGGIFGAVGQTCIAGSRLLVHRSIH 299 Query: 300 EPFMERLAAGAAALRVGDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGVRPK 359 + F+E+LA R+GDP TQ+GP+ + E + Y+D+ +G + GG RP Sbjct: 300 DEFLEKLAVFTKTARIGDPRKVETQIGPIANSMQFEKVLGYIDIARREGAELILGGGRPD 359 Query: 360 GPAFERGYFYTPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGI 419 GYF PTI GV ND RI +EE+FGPVL A+ FD+ E+ + ANDS + L AG+ Sbjct: 360 LEECGTGYFIEPTIFAGVSNDMRIAREEVFGPVLSAIVFDEPEEALAIANDSEFGLGAGV 419 Query: 420 WTRDYKRAWRVGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAM 479 WT D + A ++ ++AG+VW+NTY+ S +TPFGG++ SG+GRE G GI +Y++ KA+ Sbjct: 420 WTSDMRLALKMSERLEAGSVWVNTYRDISYTTPFGGYKKSGIGRENGVAGIYEYLQTKAV 479 Query: 480 YWGLNEQ 486 + E+ Sbjct: 480 WLSTAEE 486 >BH0539 [C] COG1012 NAD-dependent aldehyde dehydrogenases Bha Bacillus halodurans Length = 498 Score = 384 bits (985), Expect = e-106, Method: Compositional matrix adjust. Identities = 195/479 (40%), Positives = 291/479 (60%), Gaps = 6/479 (1%) Query: 8 QPICIAGQWRPG-AGDRYATLYPATGEAVASLHAASVADVEEAILGADRAFRTSGWAQKK 66 + + I G+W +G + T+ PATGE +A + A D++ A+ A +AF W++ Sbjct: 24 KKLFINGEWVDSFSGQSFETMNPATGETLALVAEAGSEDIDFAVKAARKAFDEGPWSKMS 83 Query: 67 PHERAAVLFRVAQLIRENGEALAQLQRLDNGKPISETRNL-VASAAATFQFFAAACETLE 125 ER+ +++++A LI ++ EALAQL+ LDNGKPI ET N + F++FA + Sbjct: 84 AAERSRLIYKLADLIEDHKEALAQLETLDNGKPIRETANADIPLVIEHFRYFAGWATKIV 143 Query: 126 ESLTPMRGDNLTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLA 185 P++G + HE +GVV I PWN P+ A K+ ALA G VV+KPAE TPL+ Sbjct: 144 GQTIPVQGHYFNYTRHEAIGVVGQIIPWNFPLLMAAWKLGAALATGCTVVLKPAEQTPLS 203 Query: 186 ALALARLCIEAGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHIAADK 245 AL L L EAG P G+++++PG G G + HP V +V+FTG T GK I A++ Sbjct: 204 ALYLGELINEAGFPKGVVNIVPGFGHSAGTPLVNHPAVDKVAFTGSTDVGKLIMKQASES 263 Query: 246 MMPVSLELGGKSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHEPFMER 305 + V+LELGGKSP ++ DAD+ AV G L GI + G+ C AGSRL+V + ++ + Sbjct: 264 LKRVTLELGGKSPNIILPDADLSKAVPGSLMGIMFNQGQVCCAGSRLYVQKKQYDHVVAD 323 Query: 306 LAAGAAALRVGDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGVRPKGPAFER 365 L + ++ GD E+T MGPLI+ + + ++ Y+D GV +G + TGG P +++ Sbjct: 324 LVSLTNEIQQGDGLLEQTTMGPLISNQQQSRVKNYIDRGVEEGAELLTGGNIP----YDQ 379 Query: 366 GYFYTPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWTRDYK 425 GYF PTI V + I +EEIFGPV+ AMPFD +DLI +ANDS Y LAAG+WT+D K Sbjct: 380 GYFVAPTIFADVDHSMTIAKEEIFGPVVAAMPFDSIDDLIDKANDSDYGLAAGVWTQDIK 439 Query: 426 RAWRVGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMYWGLN 484 +A + ++AG +W+N Y F ++PFGG++ SG+GRE G + Y E K+++ ++ Sbjct: 440 KAHYIAHRIKAGTIWVNCYNVFDAASPFGGYKQSGIGREMGSYALENYTEVKSVWVNMD 498 >YER073w [C] COG1012 NAD-dependent aldehyde dehydrogenases Sce Saccharomyces cerevisiae Length = 520 Score = 383 bits (984), Expect = e-106, Method: Compositional matrix adjust. Identities = 187/462 (40%), Positives = 286/462 (61%), Gaps = 5/462 (1%) Query: 24 YATLYPATGEAVASLHAASVADVEEAILGADRAFRTSGWAQKKPHERAAVLFRVAQLIRE 83 + + P+ E + +++ A DV+EA+ A +AF T W+ +P RA LF +A L+ + Sbjct: 61 FDVINPSNEEKITTVYKAMEDDVDEAVAAAKKAFETK-WSIVEPEVRAKALFNLADLVEK 119 Query: 84 NGEALAQLQRLDNGKPISETRNLVASAAATFQFFAAACETLEESLTPMRGDNLTMSVHEP 143 + E LA ++ +DNGK + R VA + + + + ++ ++ T S+ EP Sbjct: 120 HQETLAAIESMDNGKSLFCARGDVALVSKYLRSCGGWADKIYGNVIDTGKNHFTYSIKEP 179 Query: 144 MGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEVTPLAALALARLCIEAGVPAGLI 203 +GV I PWN P+ + K+ PALA GN VV+KPAE TPL+AL ++LC EAG+PAG++ Sbjct: 180 LGVCGQIIPWNFPLLMWSWKIGPALATGNTVVLKPAETTPLSALFASQLCQEAGIPAGVV 239 Query: 204 SVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHIAADKMMPVSLELGGKSPTMVFE 263 ++LPG G V+G+ ++ HP VK+++FTG T+TG+HI +AAD + V+LELGGKSP +VF Sbjct: 240 NILPGSGRVVGERLSAHPDVKKIAFTGSTATGRHIMKVAADTVKKVTLELGGKSPNIVFA 299 Query: 264 DADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHEPFMERLAAGAAALRVGDPADERT 323 DAD+D AV + +GIF +SGE C AGSR+++ +++E +++L +L+VGDP DE Sbjct: 300 DADLDKAVKNIAFGIFYNSGEVCCAGSRIYIQDTVYEEVLQKLKDYTESLKVGDPFDEEV 359 Query: 324 QMGPLITARHREGIERYVDMGVTDGGRIRTGGVRPKGPAFERGYFYTPTIIEGVRNDQRI 383 G + + I YVD+ ++G R+ TGG R +GYF PT+ V+ D RI Sbjct: 360 FQGAQTSDKQLHKILDYVDVAKSEGARLVTGGARHG----SKGYFVKPTVFADVKGDMRI 415 Query: 384 CQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWTRDYKRAWRVGRAVQAGNVWINT 443 +EE+FGP++ F +++I ANDS Y LAAGI T D +A V + V+AG VWINT Sbjct: 416 VKEEVFGPIVTVSKFSTVDEVIAMANDSQYGLAAGIHTNDINKAVDVSKRVKAGTVWINT 475 Query: 444 YKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMYWGLNE 485 Y F + PFGG+ SG+GRE G + Y + K++ +++ Sbjct: 476 YNNFHQNVPFGGFGQSGIGREMGEAALSNYTQTKSVRIAIDK 517 >BS_dhaS [C] COG1012 NAD-dependent aldehyde dehydrogenases Bsu Bacillus subtilis Length = 495 Score = 379 bits (974), Expect = e-105, Method: Compositional matrix adjust. Identities = 194/484 (40%), Positives = 292/484 (60%), Gaps = 6/484 (1%) Query: 4 LNDIQPICIAGQWRPGA-GDRYATLYPATGEAVASLHAASVADVEEAILGADRAFRTSGW 62 L + + I G++ P A G + T PATGE + +L+ A ADV++A+ A +AF W Sbjct: 16 LQGTKKLYIDGKFVPSASGATFDTPNPATGETLMTLYEAQAADVDKAVKAARKAFDQGEW 75 Query: 63 AQKKPHERAAVLFRVAQLIRENGEALAQLQRLDNGKPISETRN-LVASAAATFQFFAAAC 121 P R+ +++++A L+ E+ LAQL+ LDNGKPI+ET N + A +++A C Sbjct: 76 RTMSPASRSRLMYKLADLMEEHKTELAQLETLDNGKPINETTNGDIPLAIEHMRYYAGWC 135 Query: 122 ETLEESLTPMRGDNLTMSVHEPMGVVAAITPWNSPIASEAQKMAPALAAGNAVVVKPAEV 181 + P+ G + HEP+GVV I PWN P+ KM ALA G +V+KPAE Sbjct: 136 TKITGQTIPVSGAYFNYTRHEPVGVVGQIIPWNFPLLMAMWKMGAALATGCTIVLKPAEQ 195 Query: 182 TPLAALALARLCIEAGVPAGLISVLPGKGSVIGDAITLHPLVKRVSFTGGTSTGKHIAHI 241 TPL+AL LA L +AG PAG+I+++PG G G+A+T H V +++FTG T GK I Sbjct: 196 TPLSALYLAELIDQAGFPAGVINIIPGFGEDAGEALTNHEAVDKIAFTGSTEIGKKIMST 255 Query: 242 AADKMMPVSLELGGKSPTMVFEDADIDHAVAGVLYGIFSSSGESCIAGSRLFVARSLHEP 301 AA + V+LELGGKSP ++ DA++ A+ G L G+ + G+ C AGSR+F+ + ++ Sbjct: 256 AAKSIKRVTLELGGKSPNILLPDANLKKAIPGALNGVMFNQGQVCCAGSRVFIHKDQYDE 315 Query: 302 FMERLAAGAAALRVGDPADERTQMGPLITARHREGIERYVDMGVTDGGRIRTGGVRPKGP 361 ++ +A+ A +LR G + TQ+GPL++ E + Y+ G +G + TGG P Sbjct: 316 VVDEMASYAESLRQGAGLHKDTQIGPLVSKEQHERVLSYIQKGKDEGAKAVTGGSCP--- 372 Query: 362 AFERGYFYTPTIIEGVRNDQRICQEEIFGPVLVAMPFDDEEDLIRQANDSVYALAAGIWT 421 FE GYF PT+ V ++ I +EEIFGPVL A+P++ +++I +AN S Y LAAG+WT Sbjct: 373 -FEAGYFVAPTVFANVEDEMTIAKEEIFGPVLTAIPYETVDEVIERANHSEYGLAAGLWT 431 Query: 422 RDYKRAWRVGRAVQAGNVWINTYKQFSISTPFGGWRDSGLGREKGRLGILQYMEQKAMYW 481 + K+A + +QAG VW+N Y F ++PFGG++ SGLGRE G + Y E K+++ Sbjct: 432 ENVKQAHYIADRLQAGTVWVNCYNVFDAASPFGGYKQSGLGREMGSYALDNYTEVKSVWV 491 Query: 482 GLNE 485 L + Sbjct: 492 NLED 495