BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0088.p (447 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4343 [GEPR] COG0477 Permeases of the major facilitator superfa... 283 8e-76 RSc3059 [GEPR] COG0477 Permeases of the major facilitator superf... 268 2e-71 RSp0536 [GEPR] COG0477 Permeases of the major facilitator superf... 265 2e-70 RSc3060 [GEPR] COG0477 Permeases of the major facilitator superf... 263 6e-70 PA5476 [GEPR] COG0477 Permeases of the major facilitator superfa... 239 7e-63 >PA4343 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 439 Score = 283 bits (723), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 172/420 (40%), Positives = 253/420 (60%), Gaps = 17/420 (4%) Query: 21 APMSAARTRRLIVASSIGNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLS 80 P +RTR+ + A+ IGNALEW+DF+ YGF + I++LFFP+GDE SLL+ ATFG+ Sbjct: 4 CPSGTSRTRQ-VAAAVIGNALEWYDFIVYGFLSSLIARLFFPSGDEYTSLLMALATFGVG 62 Query: 81 YLFRPLGAILLGWYGDKVGRKPAL-LVSLSMTVAGTLICTVIPTYETIGVAAPIGILVAR 139 + RP+G +LLG Y D+ GRK A+ L+ L MT++ +I PTY IGV AP+ I++AR Sbjct: 63 FFMRPVGGVLLGLYADRRGRKAAMQLIILLMTLSIAMIA-FAPTYAAIGVGAPLLIVIAR 121 Query: 140 ILLALSAGGEFGSATALLTETG-SKRRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQ 198 +L + GGE+ SATA L E+ RR SWQ Q A A G L+ L E Sbjct: 122 MLQGFATGGEYASATAFLVESAPPHRRGLYGSWQLFGQCLAVFAGAGMGALVTHCLDAEA 181 Query: 199 ISAWGWRLPFAFGLLIAPVGLYIRRHIDEVPMAKKA-QVAGEDDASWG-LQVTR--VLVA 254 + +WGWR+PF FGLLI PVGL+IRR++ E +A + GE G L+ R VLV+ Sbjct: 182 LESWGWRVPFLFGLLIGPVGLWIRRYMGETEAFLEAIREPGERQGLLGVLREYRRSVLVS 241 Query: 255 MGILSVSTAATYLMLLYMPTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFM 314 MG+ V T + Y++L+ MPT+A KQL LP ++ ++ AAV A++ + P Sbjct: 242 MGLTVVGTVSFYVVLVNMPTFAHKQLGLPLDE-VFMVQM------AAV--ALMTLVIPLA 292 Query: 315 GMLADRVGRARIMVVSTVLTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGV 374 G L+DRVGR +++V+T+ + +Y F WV A P++ ++ + + +FG Sbjct: 293 GGLSDRVGRRPVLLVATLAFMLMVYPLFAWVAAAPSLGRLLLMQLLLCTAIGGFFGPAPT 352 Query: 375 VLSSIFPPATRVRGMGISYSLGATLFGGFTPFMVTWLVGKTGNPVSPSYYLMVCAAISLL 434 ++ FP R G+ ++Y+L LFGGF PF+VTWL G+PV+P++Y++ A + LL Sbjct: 353 AVAEQFPVRVRSTGLAVAYNLAVMLFGGFAPFIVTWLTEVGGSPVAPAFYVLGAAFLGLL 412 >RSc3059 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 431 Score = 268 bits (685), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 153/406 (37%), Positives = 228/406 (56%), Gaps = 16/406 (3%) Query: 38 GNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYLFRPLGAILLGWYGDK 97 GN LE +DF+ YG++A I+ FFP +E +SL+++ TFG+ +L RPLGA++LG Y D+ Sbjct: 20 GNFLEMYDFMVYGYYAKAIADTFFPADNEFLSLMLSLVTFGVGFLMRPLGALILGAYIDR 79 Query: 98 VGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILLALSAGGEFGSATALL 157 GR+ L+++L + GTL+ ++P Y TIG+AAP+ +L+ R+L SAG E G + L Sbjct: 80 HGRRAGLILTLGLMACGTLLIALVPGYATIGLAAPLLVLIGRLLQGFSAGVELGGVSVYL 139 Query: 158 TETGSK-RRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISAWGWRLPFAFGLLIAP 216 E + R+ F SWQ +SQ A + AAL G LL+ L+P+++ WGWR+PF G LI P Sbjct: 140 AEISTPGRKGFFVSWQSASQQVAVMFAALLGVLLSFQLSPKEMGEWGWRVPFLVGCLIVP 199 Query: 217 VGLYIRRHIDEVPMAKKAQ---VAGEDDASWGLQVTRVLVAMGILSVSTAATYLMLLYMP 273 IRR + E K+ + GE S G V ++ ++T + YL+ Y P Sbjct: 200 FLFLIRRSLQETEEFKQRKHRPALGEIFRSMGENWRLVGAGTLLVVMTTVSFYLITAYTP 259 Query: 274 TYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGMLADRVGRARIMVVSTVL 333 T+ LHL +L T GA P MG ++DR+GR +++ T L Sbjct: 260 TFGKSVLHLSNLDSLIVT---------LCVGASNFFWLPVMGSVSDRIGRRPLLITFTAL 310 Query: 334 TLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVLSSIFPPATRVRGMGISY 393 TL T Y + W+IA P+ ++A+ +W L Y G++ V L+ I PPA R G ++Y Sbjct: 311 TLLTAYPAMQWLIAEPSFGRLLAVELWLSFLYGSYNGAMVVTLTEIMPPAVRTTGFSLAY 370 Query: 394 SLGATLFGGFTPFMVTWLVGKTGNPVSPSYYL---MVCAAISLLCV 436 SL +FGGFTP + TWL+ TGN P +L +C ++ L V Sbjct: 371 SLATAIFGGFTPAIATWLIHATGNKAMPGLWLSFAAICGLVATLIV 416 >RSp0536 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 441 Score = 265 bits (677), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 157/414 (37%), Positives = 226/414 (54%), Gaps = 16/414 (3%) Query: 27 RTRRLIVASSIGNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYLFRPL 86 R R + + +GNA EWFDF YGFFA I+ + FP+ + +LL+ ATFG+ ++ RPL Sbjct: 17 RQRHAVATAILGNAFEWFDFTLYGFFAPVIATVLFPSANPFTALLLAIATFGVGFVTRPL 76 Query: 87 GAILLGWYGDKVGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILLALSA 146 G I+LG Y D+ GR+PAL +S + GT + + PTY G+AAP+ ++AR+L SA Sbjct: 77 GGIVLGIYADRRGRRPALALSALLMALGTALIAIAPTYAQAGIAAPVIAVIARLLQGFSA 136 Query: 147 GGEFGSATALLTETGSK-RRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISAWGWR 205 GGE G ATA L E +RAF SW +S G A + A+ G L L E + AWGWR Sbjct: 137 GGEMGGATAYLNEIAPPGKRAFYTSWIQASVGLAIVCGAMLGTLATSLLDAEALRAWGWR 196 Query: 206 LPFAFGLLIAPVGLYIRRHIDEVPMAKKAQVAGEDDASWGLQVTR-----VLVAMGILSV 260 +PFA G+LI P+G IR +DE P +A D+ L+V R VL+ I+ + Sbjct: 197 IPFALGVLIGPIGYVIRSRLDETPAFARAATEARADSPL-LEVVRRHRRAVLIGASIVIL 255 Query: 261 STAATYLMLLYMPTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGMLADR 320 T TY++L YM TYAI L LP S + +G +M+ AP G LADR Sbjct: 256 WTVCTYVLLFYMQTYAIHVLKLPSSAGFAGSM---------ASGVTMMLTAPLFGWLADR 306 Query: 321 VGRARIMVVSTVLTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVLSSIF 380 +G R++ + + L Y + ++ P ++ + I G+L A Y G + V + +F Sbjct: 307 LGYQRVLSGAALAILVLAYPLYAYLNHAPAAGTLIGVQIVFGLLSAAYTGPIPVAFADLF 366 Query: 381 PPATRVRGMGISYSLGATLFGGFTPFMVTWLVGKTGNPVSPSYYLMVCAAISLL 434 P G+ +Y+L L GGF PF +TWL ++P Y+MV A +SL+ Sbjct: 367 PTKVLSTGLSTAYNLTVMLIGGFAPFYLTWLSHAFDPMLAPVLYVMVAALVSLI 420 >RSc3060 [GEPR] COG0477 Permeases of the major facilitator superfamily Rso Ralstonia solanacearum Length = 433 Score = 263 bits (672), Expect = 6e-70, Method: Compositional matrix adjust. Identities = 157/407 (38%), Positives = 232/407 (57%), Gaps = 18/407 (4%) Query: 38 GNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYLFRPLGAILLGWYGDK 97 GN LE FDF+ YG++A I+ FFP G+ +SL+++ TFG +L RPLGAI+LG Y D+ Sbjct: 20 GNFLEMFDFMVYGYYATAIAATFFPAGNAFLSLMLSLMTFGAGFLMRPLGAIVLGAYIDR 79 Query: 98 VGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILLALSAGGEFGSATALL 157 GR+ L+++L + GT++ ++P Y TIG+AAP+ +L+ R+L SAGGE G + L Sbjct: 80 HGRRAGLILTLVLMACGTMLIALVPGYGTIGLAAPLLVLIGRLLQGFSAGGEPGGVSVYL 139 Query: 158 TETGSK-RRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISAWGWRLPFAFGLLIAP 216 +E + R+ F SWQ SQ A + AAL G LL+ L+P+++ WGWR+PF G LI P Sbjct: 140 SEIATPGRKGFYVSWQSGSQQVAVMFAALLGVLLSFKLSPKEMGEWGWRVPFLIGCLIVP 199 Query: 217 VGLYIRRHI---DEVPMAKKAQVAGEDDASWGLQVTRVLVAMGILSV-STAATYLMLLYM 272 +IRR + +E K GE S G + R+ A +L+V +T + YL+ Y Sbjct: 200 FLFFIRRSLRETEEFEQRKHRPTMGELLRSMG-ENWRLAGAGTLLAVMTTVSFYLITAYT 258 Query: 273 PTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGMLADRVGRARIMVVSTV 332 PT+ LH+ +L T GA +I P MG L+DR+GR ++V + Sbjct: 259 PTFGRNVLHMSDFDSLIVT---------LCVGASNLIWLPVMGSLSDRIGRRTPLIVFSA 309 Query: 333 LTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVLSSIFPPATRVRGMGIS 392 LTL T Y + W+IA P+ ++A+ +W L Y G++ V L+ I PP R G ++ Sbjct: 310 LTLLTAYPAMTWLIAEPSFGRLLAVELWLSFLYGGYNGAMIVTLTEIMPPQLRTTGFSLA 369 Query: 393 YSLGATLFGGFTPFMVTWLVGKTGNPVSPSYYL---MVCAAISLLCV 436 YSL +FGGFTP + T L+ TGN P +L +C ++ L V Sbjct: 370 YSLATAIFGGFTPAIATGLIHATGNKAMPGLWLSFAAICGLVATLTV 416 >PA5476 [GEPR] COG0477 Permeases of the major facilitator superfamily Pae Pseudomonas aeruginosa Length = 429 Score = 239 bits (611), Expect = 7e-63, Method: Compositional matrix adjust. Identities = 142/392 (36%), Positives = 215/392 (54%), Gaps = 21/392 (5%) Query: 38 GNALEWFDFVAYGFFAVTISKLFFPTGDETVSLLVTFATFGLSYLFRPLGAILLGWYGDK 97 GN LE +DF+ YGF+A I+K FFP + SL+++ ATFG +L RPLGA+ LG Y D+ Sbjct: 17 GNFLEMYDFMVYGFYATAIAKTFFPGDNPFASLMLSLATFGAGFLMRPLGAVFLGAYIDR 76 Query: 98 VGRKPALLVSLSMTVAGTLICTVIPTYETIGVAAPIGILVARILLALSAGGEFGSATALL 157 GR+ L+++L + GTL+ +P Y T+GVAAP+ +L R+L SAG E G + L Sbjct: 77 HGRRQGLIITLGLMAMGTLLIAFVPGYATLGVAAPLLVLFGRLLQGFSAGVELGGVSVYL 136 Query: 158 TETGS-KRRAFMASWQFSSQGAAALMAALAGFLLNQWLTPEQISAWGWRLPFAFGLLIAP 216 +E + R+ F SWQ +SQ A + A L G LLNQWL+P+ + WGWR+PF G LI P Sbjct: 137 SEIATPGRKGFFVSWQSASQQVAVVFAGLLGVLLNQWLSPQDMGEWGWRVPFFIGCLIVP 196 Query: 217 VGLYIRRHIDEVPMAKK-------AQVAGEDDASWGLQVTRVLVAMGILSVSTAATYLML 269 IRR ++E P + +QV ++G+ VL ++ ++T + YL+ Sbjct: 197 ALFVIRRSLEETPEFEARTHRPSLSQVLRSIGQNFGV----VLAGTAMVVMTTVSFYLIT 252 Query: 270 LYMPTYAIKQLHLPQSTALYRTRLKKTPPPAAVTGAILMIGAPFMGMLADRVGRARIMVV 329 Y PT+ +L L +L T G I P MG +DR+GR +++ Sbjct: 253 AYTPTFGKNELQLSDLDSLLVT---------MCIGLSNFIWLPVMGAFSDRIGRKPLLLG 303 Query: 330 STVLTLATIYGSFVWVIAMPTVWVMMAIMIWAGILKAVYFGSLGVVLSSIFPPATRVRGM 389 ++ L L T Y + W++ P+ ++ + +W L + Y G++ V L+ + P R G Sbjct: 304 ASALALLTAYPALSWLVREPSFAGLLGVELWLSFLYSSYNGAMVVALTEVMPADVRTTGF 363 Query: 390 GISYSLGATLFGGFTPFMVTWLVGKTGNPVSP 421 ++YSL FGGFTP + TWL+ N +P Sbjct: 364 SLAYSLATATFGGFTPAVCTWLIHTLDNRAAP 395