BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0061.p (249 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC922.06 [IQR] COG1028 Dehydrogenases with different specifici... 96 5e-20 ML1807 [IQR] COG1028 Dehydrogenases with different specificities... 91 2e-18 BMEII0080 [IQR] COG1028 Dehydrogenases with different specificit... 84 3e-16 PA5150 [IQR] COG1028 Dehydrogenases with different specificities... 83 6e-16 alr4456 [IQR] COG1028 Dehydrogenases with different specificitie... 83 7e-16 >SPAC922.06 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Spo Schizosaccharomyces pombe Length = 258 Score = 96.3 bits (238), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 75/259 (28%), Positives = 125/259 (48%), Gaps = 31/259 (11%) Query: 4 RHFLVVGGSKGSGLALVEHLLAAGQRVS-VLARSASPLPSSTLLNWQQLDVTDGGALRAA 62 R L+ G + G G L + G RV+ + S + + L Q DV+ + A Sbjct: 6 RVVLITGAAGGIGKVLCKMFTELGDRVAGIDIVDPSKVQDAALA--LQADVSKADQIETA 63 Query: 63 LDACARSQGKIDSAV----------FMQRNRATGDAFDTDLAVALTAT----RNAIDHLL 108 ++ ++ G ID + F Q + +++D D+++ L R I H+ Sbjct: 64 IEKVIQTLGPIDVLINNAGLADDTPFEQLSH---ESWDHDVSLVLRGNYLTQRYVIPHMA 120 Query: 109 AQGHFSLDESGNSIVMVSSIADH-YVAPEQDLGYHVSKAGLVQLARYYALQLGPHGIRVN 167 QG G SIV + S+ H Y+ Y +KAGL L + A++ GP GIRVN Sbjct: 121 KQG------KGGSIVNIGSVNGHIYLGSP---AYSAAKAGLENLTKALAVRYGPLGIRVN 171 Query: 168 VVSPCVVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSSYITGQN 227 V +P + A E ++++ + DR +R+ P+ R G+ D+ A++FL ++S+ITG Sbjct: 172 VCAPGTIWSPAWDERFKKHPDVGDRMKRWYPVGRLGTPEDVARAVIFLADSKNSFITGTT 231 Query: 228 IVVDGGLSLRSHESIIRDF 246 + VDGGL L + +I+D Sbjct: 232 LYVDGGL-LAGNPCLIQDI 249 >ML1807 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mle Mycobacterium leprae Length = 253 Score = 91.3 bits (225), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 84/251 (33%), Positives = 118/251 (47%), Gaps = 39/251 (15%) Query: 4 RHFLVVGGSKGSGLALVEHLLAAGQRVSVLARSASPLPSSTLLNW---QQLDVTDGGALR 60 R LV GG++G GLA+ L+A G RV+V R AS +P +W + DVTD A+ Sbjct: 22 RSILVTGGNRGIGLAVARRLVADGHRVAVTHR-ASVVP-----DWFFGVECDVTDNDAIG 75 Query: 61 AALDACARSQGKIDSAVF-------MQRNRATGDAFDTDLAVALT--------ATRNAID 105 AA A QG ++ V M R + + F + V LT A+RN I Sbjct: 76 AAFKAVEEHQGPVEVLVANAGITSDMLIMRMSEEQFQKVIDVNLTGVFRVAKLASRNMIK 135 Query: 106 HLLAQGHFSLDESGNSIVMVSSIADHYVAPEQDLGYHVSKAGLVQLARYYALQLGPHGIR 165 + F S+V +S + P Q Y SK+GL+ +AR A +LG I Sbjct: 136 QRFGRYIFM-----GSVVGLSGV------PGQ-ANYAASKSGLIGMARSLARELGSRSIT 183 Query: 166 VNVVSPCVVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSSYITG 225 NVV+P + E R +S + + IPL R G+ +I A+ FL E + YI+G Sbjct: 184 ANVVAPGFIDTEMTRA---MDSKYQEAALQAIPLGRIGAVEEIAGAVSFLASEDAGYISG 240 Query: 226 QNIVVDGGLSL 236 I VDGGL + Sbjct: 241 AVIPVDGGLGM 251 >BMEII0080 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bme Brucella melitensis Length = 271 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 74/246 (30%), Positives = 114/246 (46%), Gaps = 18/246 (7%) Query: 4 RHFLVVGGSKGSGLALVEHLLAAGQRVSVLARSASPLPSSTLLNWQQLDVTDGGALRAAL 63 + +V G ++G G + EH LA G V L R A + +LD+TD +RA Sbjct: 29 KRVIVTGAAQGIGRRVAEHFLAEGATVIGLDRQAGE--ADAPFRLIRLDITDAAEVRAVS 86 Query: 64 DACARSQGKIDSAVFMQRNRATGDA---FDTDLAVALTATRNAIDHLLAQG-HFSLDESG 119 D +D V + G++ D + + HLL+Q D+ Sbjct: 87 DGLKAEGKPLDILVNVAGVLKLGNSDRLSAEDWKTCMDVNASGPFHLLSQWVPVFRDQRH 146 Query: 120 NSIVMVSSIADHYVAPEQDLG-YHVSKAGLVQLARYYALQLGPHGIRVNVVSPC------ 172 +IV V+S A H P ++ Y SKA L + AL+L P+G+R NVVSP Sbjct: 147 GAIVNVASNAAHV--PRLNMAAYCASKAALASFSHCVALELAPYGVRCNVVSPGSTRTAM 204 Query: 173 --VVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSSYITGQNIVV 230 ++ + + E + ++F+ IPL + DI N ILFL +Q+ ++T Q+IVV Sbjct: 205 LGAMLNDPSGEAQLVRG-LPEQFKLGIPLGKIAIPDDIANVILFLASDQAGHVTMQDIVV 263 Query: 231 DGGLSL 236 DGG +L Sbjct: 264 DGGATL 269 >PA5150 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 245 Score = 82.8 bits (203), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 79/252 (31%), Positives = 113/252 (44%), Gaps = 29/252 (11%) Query: 1 MSERH-FLVVGGSKGSGLALVEHLLAAGQRVSVLARS--ASPLP-SSTLLNWQQLDVTDG 56 M+ER L+ G S+G G A V++ AAG RV +R AS P + N LD+ D Sbjct: 1 MAERKTLLLTGASRGIGHATVKYFNAAGWRVFTASRQSWASECPWADGEENHIHLDLEDI 60 Query: 57 GALRAALDACARSQGK------IDSAVFMQRNRATG--DAFDTDLAVALTATRNAI--DH 106 + A+L+ G +D+A + +TD A L + Sbjct: 61 PGVEASLELIREKLGDGRLHALVDNAGISPKGEGGERLGVLETDYATWLRVFNVNLFSTA 120 Query: 107 LLAQGHFS-LDESGNSIVMVSSIADHYVAPEQDLGYHVSKAGLVQLARYYALQLGPHGIR 165 LLA+G F+ L + +++ V+SIA V P + Y SKA L L R A GPHG+R Sbjct: 121 LLARGLFAELKAAQGTVINVTSIAGSKVHPFAGVAYATSKAALSALTREMAHDFGPHGVR 180 Query: 166 VNVVSP----CVVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSS 221 VN ++P ++ E ER IPL R G +I + I FL +S Sbjct: 181 VNAIAPGEIDTSILSPGTEEI----------VERSIPLHRLGRPEEIASLIYFLCTSGAS 230 Query: 222 YITGQNIVVDGG 233 Y+ G I V+GG Sbjct: 231 YVNGAEIHVNGG 242 >alr4456 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Nos Nostoc sp. PCC 7120 Length = 267 Score = 82.8 bits (203), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 70/247 (28%), Positives = 112/247 (45%), Gaps = 17/247 (6%) Query: 1 MSERHFLVVGGSKGSGLALVEHLLAAGQRVSVLARSASPLPSSTLLNWQQL--------D 52 ++ R L+ G +KG GLA+ + LA G V ++AR+A + + W D Sbjct: 20 LAGRKALITGATKGIGLAIAQEFLALGAEVVIVARNAEAI-EQQMKAWHSAGKVHGVAAD 78 Query: 53 VTDGGALRAALDACARSQGKIDSAV----FMQRNRATGDAFDTDLAVALTATRNAIDHLL 108 V+ + LD +++ G++D V R +AT D + + A +I L Sbjct: 79 VSTSEGRQMMLDYVSKTFGELDILVNNVGTNIRKKAT-DYTEEEFAAIFQINLTSIFELS 137 Query: 109 AQGHFSLDESGNS-IVMVSSIADHYVAPEQDLGYHVSKAGLVQLARYYALQLGPHGIRVN 167 + L S NS IV + S+A ++ Y ++KA LVQL R A++ GIRVN Sbjct: 138 RLFYPLLKTSKNSSIVNIGSVAG-LISVRTGAPYGMTKAALVQLTRSLAVEWADDGIRVN 196 Query: 168 VVSPCVVIKEAAREYYRQNSWITDRFERFIPLRRAGSSRDIINAILFLTGEQSSYITGQN 227 ++P + ++ R P++R G ++ + FL +SYITGQ Sbjct: 197 AIAPWFIQTPLTEPLLNNPETLSAVLSR-TPMKRVGQPEEVASLTAFLCMPTASYITGQC 255 Query: 228 IVVDGGL 234 I VDGG Sbjct: 256 IAVDGGF 262