BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0030.0091.p (605 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2216 [L] COG0358 DNA primase (bacterial type) Rso Ralstonia s... 663 0.0 NMA1736 [L] COG0358 DNA primase (bacterial type) NmA Neisseria m... 457 e-128 NMB1537 [L] COG0358 DNA primase (bacterial type) Nme Neisseria m... 456 e-128 PA0577 [L] COG0358 DNA primase (bacterial type) Pae Pseudomonas ... 411 e-114 VC0518 [L] COG0358 DNA primase (bacterial type) Vch Vibrio cholerae 409 e-114 >RSc2216 [L] COG0358 DNA primase (bacterial type) Rso Ralstonia solanacearum Length = 603 Score = 663 bits (1711), Expect = 0.0, Method: Compositional matrix adjust. Identities = 350/603 (58%), Positives = 424/603 (70%), Gaps = 25/603 (4%) Query: 1 VIPQSFIQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG 60 +IPQ FI DLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG Sbjct: 1 MIPQPFIDDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG 60 Query: 61 CGAHGTAISFLIEYSGMGFVEAVKDLAQNVGMVVPEREDNIPLAQRQEKQAKSMALSDVM 120 CGAHG+AI FL+E+SG+ +VEA++DLAQ+VGM VPE +P A R E+QAK++AL DVM Sbjct: 61 CGAHGSAIGFLMEFSGLSYVEAIRDLAQSVGMAVPEERGGLPPAARAERQAKTVALGDVM 120 Query: 121 TRANDFYRHQLRSAQPAIAYLKGRGLTGEVAARFGLGYSPDEWDSLRQVFPDYENEV--- 177 RA+D YR QLR A A+ YLKGRGLTGE+A FGLGY+PD+W SL VF Y ++ Sbjct: 121 ARASDHYRKQLRGAPGAVQYLKGRGLTGEIAGHFGLGYAPDDWQSLESVFGAYRDDAIAT 180 Query: 178 -LVESGLVI--DKSEEGGGHRKRYDRFRGRIMFPIRNTKGQVIGFGGRVMDGGEPKYLNS 234 L+E+GLVI +K++ G HR RYDRFR RIMFPIRNTKG VIGFGGRV+ GEPKYLNS Sbjct: 181 PLIEAGLVIESEKTDADGRHR-RYDRFRDRIMFPIRNTKGVVIGFGGRVLGQGEPKYLNS 239 Query: 235 PETPLFQKGSELYGLFEARQAIREAGYVLVTEGYMDVVALAQLGFPQAVATLGTACTPIH 294 PETPLF KG+ELYGLFEAR AIRE GYVLV EGYMDVVALAQLGF AVATLGTACTP+H Sbjct: 240 PETPLFSKGTELYGLFEARNAIREFGYVLVVEGYMDVVALAQLGFANAVATLGTACTPVH 299 Query: 295 VQKLVRQTDEVIFSFDGDNAGRRAARRAAGXARRALEACLPHASDNKTIKFLFLPKEHDP 354 VQKL+RQ D VIFSF ARRALEACLPH +DNK +KFLFLP EHDP Sbjct: 300 VQKLLRQVDTVIFSF-------DGDAAGRRAARRALEACLPHVTDNKIVKFLFLPPEHDP 352 Query: 355 DSYVREMGAPAFEEQIREAMPLSQFLLKEIIGENDLRTPEGRAHAQFAAKPLLQALPASG 414 D+++RE G AF ++R AMPLS+FLL+ + + DLR PEGRA AQ+ AKPLL A+P G Sbjct: 353 DTFIREEGTEAFAAEVRNAMPLSRFLLQAVTEDLDLRQPEGRARAQYEAKPLLSAMPPGG 412 Query: 415 LRLQIVRSVAQATQTTPAELEVLFELEKPVA---RVRSAPPRTKRTAPVGLERQIMRLLV 471 LRLQIVR +A+ T TTPA++E L L A R+ PR RTAP LE++++RLL+ Sbjct: 413 LRLQIVRGLAEMTGTTPADIEALCGLRSDPAQAGRMTQKRPRVARTAPTALEQKVLRLLM 472 Query: 472 AHPQLSADLDAAALEAIARIAPDQAELLAHLVGVAQGMGEQANFAALVEHLREGGPEFDS 531 +P L+A LDA + Q E+L+ L+ G + +F A VE L G + Sbjct: 473 RYPALAARLDADTRTQLTAPELPQGEVLSGLLAECDTAGPEMHFGAFVERL--GQTPYAE 530 Query: 532 MIAEI---AAESESEFDVVRAEMAGAIRQARIRALDDEMQML---VASNLRTEEERNRYR 585 A + + E + E A+++ L E++ML + T+ ++ R R Sbjct: 531 AFAGLRVAVLNDDIELEPATQEFDAAVQKMLAEPLRRELEMLQRKMEGGHATDADKERLR 590 Query: 586 ELM 588 L+ Sbjct: 591 WLV 593 >NMA1736 [L] COG0358 DNA primase (bacterial type) NmA Neisseria meningitidis Z2491 Length = 590 Score = 457 bits (1177), Expect = e-128, Method: Compositional matrix adjust. Identities = 253/546 (46%), Positives = 332/546 (60%), Gaps = 32/546 (5%) Query: 1 VIPQSFIQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG 60 +IP FI +LL + DIVD++ V LKKGGAN+M CPFH EK+PSF+VSPTKQFYHCF Sbjct: 1 MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKTPSFSVSPTKQFYHCFS 60 Query: 61 CGAHGTAISFLIEYSGMGFVEAVKDLAQNVGMVVPE---REDNIPLAQRQEKQAKSMALS 117 CGAHG+AI F++E+ G+ F EAV+ LA VGMVVP+ + DN + R E++ K L Sbjct: 61 CGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVHGQNDNPEV--RAERKKKQQTLE 118 Query: 118 DVMTRANDFYRHQLRSAQPAIAYLKGRGLTGEVAARFGLGYSPDEWDSLRQVFPDYENEV 177 + A DFY QL+ A AYL RGL+ EV A +GLGY+PD W L QVF Y N Sbjct: 119 ETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYAPDGWQPLTQVFQPYPNTA 178 Query: 178 LVESGLVIDKSEEGGGHRKRYDRFRGRIMFPIRNTKGQVIGFGGRVMDGGEPKYLNSPET 237 LV++G+VID EG + YDRFR RIMFPIRN +GQVIGFGGRV+D +PKYLNSP+T Sbjct: 179 LVDTGMVIDN--EG----RHYDRFRHRIMFPIRNPRGQVIGFGGRVLDDSKPKYLNSPDT 232 Query: 238 PLFQKGSELYGLFEARQAIREAGYVLVTEGYMDVVALAQLGFPQAVATLGTACTPIHVQK 297 PLF KG LYGL+E R A++EAG +LV EGYMDVVALAQ G VA LGTA T HV+ Sbjct: 233 PLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGYGVAALGTATTAEHVKI 292 Query: 298 LVRQTDEVIFSFDGDNAGRRAARRAAGXARRALEACLPHASDNKTIKFLFLPKEHDPDSY 357 L+RQ D + F FDGD+AGR+A A RALE LP D+K++ FLFLP+EHDPDSY Sbjct: 293 LMRQADSIYFCFDGDSAGRKA-------AWRALENALPQLKDDKSLHFLFLPEEHDPDSY 345 Query: 358 VREMGAPAFEEQ-IREAMPLSQFLLKEIIGENDLRTPEGRAHAQFAAKPLLQALPASGLR 416 +R G FE+ + ++ PLS++ + + L T EG+A + PLL + A L Sbjct: 346 IRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTSSPLLAQITAPALA 405 Query: 417 LQIVRSVAQATQTTPAELEVLFELEKPVARVRSA----PPRTKR--TAPVGLERQIMRLL 470 + + +++ P L L E P V+ PP + + P ++RQI LL Sbjct: 406 YLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQPVMPTLVQRQIRSLL 465 Query: 471 VAHPQLSADLDAAALEAIARIAPDQAELLAHLVGVAQGMGEQANFAALVEHLREGGPEFD 530 + +P +A +D A+ LA+L + A ++EH+R G ++ Sbjct: 466 I-NPDWAAYIDLPDYLALD----GDFACLANLAETIKNHPSVPATAQVLEHMR--GSPYE 518 Query: 531 SMIAEI 536 I I Sbjct: 519 ETINRI 524 >NMB1537 [L] COG0358 DNA primase (bacterial type) Nme Neisseria meningitidis MC58 Length = 590 Score = 456 bits (1172), Expect = e-128, Method: Compositional matrix adjust. Identities = 244/492 (49%), Positives = 314/492 (63%), Gaps = 28/492 (5%) Query: 1 VIPQSFIQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG 60 +IP FI +LL + DIVD++ V LKKGGAN+M CPFH EK+PSF+VSPTKQFYHCF Sbjct: 1 MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKTPSFSVSPTKQFYHCFS 60 Query: 61 CGAHGTAISFLIEYSGMGFVEAVKDLAQNVGMVVPE---REDNIPLAQRQEKQAKSMALS 117 CGAHG+AI F++E+ G+ F EAV+ LA VGMVVP+ + DN + R E++ K L Sbjct: 61 CGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVHGQNDNPEV--RAERKKKQQTLE 118 Query: 118 DVMTRANDFYRHQLRSAQPAIAYLKGRGLTGEVAARFGLGYSPDEWDSLRQVFPDYENEV 177 + A DFY QL+ A AYL RGL+ EV A +GLGY+PD W L QVF Y N Sbjct: 119 ETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYAPDGWQPLTQVFQPYPNTA 178 Query: 178 LVESGLVIDKSEEGGGHRKRYDRFRGRIMFPIRNTKGQVIGFGGRVMDGGEPKYLNSPET 237 LV++G+VID EG + YDRFR RIMFPIRN +GQVIGFGGRV+D +PKYLNSP+T Sbjct: 179 LVDTGMVIDN--EG----RHYDRFRHRIMFPIRNPRGQVIGFGGRVLDDSKPKYLNSPDT 232 Query: 238 PLFQKGSELYGLFEARQAIREAGYVLVTEGYMDVVALAQLGFPQAVATLGTACTPIHVQK 297 PLF KG LYGL+E R A++EAG +LV EGYMDVVALAQ G VA LGTA T HV+ Sbjct: 233 PLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGYGVAALGTATTAEHVKI 292 Query: 298 LVRQTDEVIFSFDGDNAGRRAARRAAGXARRALEACLPHASDNKTIKFLFLPKEHDPDSY 357 L+RQ D + F FDGD+AGR+A A RALE LP D+K++ FLFLP+EHDPDSY Sbjct: 293 LMRQADSIYFCFDGDSAGRKA-------AWRALENALPQLKDDKSLHFLFLPEEHDPDSY 345 Query: 358 VREMGAPAFEEQ-IREAMPLSQFLLKEIIGENDLRTPEGRAHAQFAAKPLLQALPASGLR 416 +R G FE+ + ++ PLS++ + + L T EG+A + PLL + A L Sbjct: 346 IRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTSSPLLAQITAPALA 405 Query: 417 LQIVRSVAQATQTTPAELEVLFELEKPVARVRSA----PPRTKRTAPVGL---ERQIMRL 469 + + +++ P L L E P V+ PP + + PV L +RQI L Sbjct: 406 YLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ-PVMLTLVQRQIRSL 464 Query: 470 LVAHPQLSADLD 481 L+ +P +A +D Sbjct: 465 LI-NPDWAAYID 475 >PA0577 [L] COG0358 DNA primase (bacterial type) Pae Pseudomonas aeruginosa Length = 664 Score = 411 bits (1056), Expect = e-114, Method: Compositional matrix adjust. Identities = 208/437 (47%), Positives = 295/437 (67%), Gaps = 27/437 (6%) Query: 1 VIPQSFIQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFG 60 +IPQSFI DLLNR DIV+VV +QLKK G N+ CPFH EK+PSFTVSP KQFY+CFG Sbjct: 4 LIPQSFIDDLLNRTDIVEVVSSRIQLKKTGKNYSACCPFHKEKTPSFTVSPDKQFYYCFG 63 Query: 61 CGAHGTAISFLIEYSGMGFVEAVKDLAQNVGMVVPERE----DNIPLAQRQEKQAKSMAL 116 CGA G A+ F++++ + F +AV++LA+ GM VP E + P +Q L Sbjct: 64 CGAGGNALGFVMDHDQLEFPQAVEELAKRAGMDVPREERGGRGHTP------RQPTDSPL 117 Query: 117 SDVMTRANDFYRHQLRSA---QPAIAYLKGRGLTGEVAARFGLGYSPDEWDSLRQVF--P 171 +++ A +FY+ L+S + A+ YLKGRGLTGE+A FGLG++P WD+L + Sbjct: 118 YPLLSAAAEFYKQALKSHPARKAAVNYLKGRGLTGEIARDFGLGFAPPGWDNLLKHLGGD 177 Query: 172 DYENEVLVESGLVIDKSEEGGGHRKRYDRFRGRIMFPIRNTKGQVIGFGGRVMDGGEPKY 231 + + + ++++GL+++ S+ G KRYDRFR R+MFPIR+++G++I FGGRV+ +PKY Sbjct: 178 NLQLKAMLDAGLLVENSDTG----KRYDRFRDRVMFPIRDSRGRIIAFGGRVLGDDKPKY 233 Query: 232 LNSPETPLFQKGSELYGLFEARQAIREAGYVLVTEGYMDVVALAQLGFPQAVATLGTACT 291 LNSPETP+F KG ELYGL+EARQ R+ ++V EGYMDV+ALAQ G AVATLGTA + Sbjct: 234 LNSPETPVFHKGQELYGLYEARQKNRDLDEIMVVEGYMDVIALAQQGIRNAVATLGTATS 293 Query: 292 PIHVQKLVRQTDEVIFSFDGDNAGRRAARRAAGXARRALEACLPHASDNKTIKFLFLPKE 351 H+++L R ++F FDGD AGR+A A RALE+ LP+ D K ++FLFLP+ Sbjct: 294 EEHIKRLFRLVPSILFCFDGDQAGRKA-------AWRALESVLPNLQDGKRVRFLFLPEG 346 Query: 352 HDPDSYVREMGAPAFEEQI-REAMPLSQFLLKEIIGENDLRTPEGRAHAQFAAKPLLQAL 410 DPDS VR G AF +I ++A PL+++ ++++ E D T EG+AH A PLL+ + Sbjct: 347 EDPDSLVRAEGEDAFRARITQQAQPLAEYFFQQLMLEADPATLEGKAHLATLAAPLLEKI 406 Query: 411 PASGLRLQIVRSVAQAT 427 P + LRL + + +++ T Sbjct: 407 PGNNLRLLMRQRLSEIT 423 >VC0518 [L] COG0358 DNA primase (bacterial type) Vch Vibrio cholerae Length = 587 Score = 409 bits (1052), Expect = e-114, Method: Compositional matrix adjust. Identities = 217/454 (47%), Positives = 290/454 (63%), Gaps = 34/454 (7%) Query: 2 IPQSFIQDLLNRVDIVDVVGRYVQLKKGGANFMGLCPFHNEKSPSFTVSPTKQFYHCFGC 61 IP+SFI DLL R+DIVDV+ V+LKK G N+ CPFHNEK+PSF+VS KQFYHCFGC Sbjct: 5 IPRSFIDDLLARLDIVDVIDARVKLKKKGKNYSACCPFHNEKTPSFSVSQEKQFYHCFGC 64 Query: 62 GAHGTAISFLIEYSGMGFVEAVKDLAQNVGMVV--PEREDNIPLAQRQE--KQAKSMALS 117 GAHG AI F++EY M F EA+++LA +G+ V ER + P A+ K +L Sbjct: 65 GAHGNAIDFVMEYERMEFPEAIEELASTIGLEVQREERTPSSPFAKNTPAVKSEDKRSLY 124 Query: 118 DVMTRANDFYRHQLR--SAQPAIAYLKGRGLTGEVAARFGLGYSPDEWDSLRQVF-PDYE 174 D+M FYR QL+ + + AI YLK RGL+GE+ +FG+GY DEWD +R+ F Sbjct: 125 DLMGSIAQFYRQQLKVPANKHAIEYLKNRGLSGEIVQKFGIGYIADEWDLVRRNFGQQRS 184 Query: 175 NEVLVESGLVIDKSEEGGGHRKRYDRFRGRIMFPIRNTKGQVIGFGGRVMDGGEPKYLNS 234 +E ++ + +++ +++ G +RYDRFRGR+MFPIR+ +G+VIGFGGR+ + G PKYLNS Sbjct: 185 SEDMLVTAVMLIENDNG----RRYDRFRGRVMFPIRDRRGRVIGFGGRITEQGTPKYLNS 240 Query: 235 PETPLFQKGSELYGLFEARQAIREAGYVLVTEGYMDVVALAQLGFPQAVATLGTACTPIH 294 PETP+F KG ELYGL+E QA RE +LV EGYMDVVALAQ G +VA+LGT+ T H Sbjct: 241 PETPIFHKGKELYGLYEVLQAYREPPQILVVEGYMDVVALAQYGVDYSVASLGTSTTGDH 300 Query: 295 VQKLVRQTDEVIFSFDGDNAGRRAARRAAGXARRALEACLPHASDNKTIKFLFLPKEHDP 354 +Q L RQT+ VI +DGD AGR A A RALE L + +KFLFLP DP Sbjct: 301 LQLLFRQTNHVICCYDGDRAGRDA-------AWRALENALSYLKSGNILKFLFLPDGEDP 353 Query: 355 DSYVREMGAPAFEEQIREAMPLSQFLLKEII--GENDLRTPEGRAHAQFAAKPLLQALPA 412 DSYVR+ G FE+Q+ A PLSQ+L + +I + +L + EG++ + A PL+ +P Sbjct: 354 DSYVRKYGKADFEQQVANATPLSQYLFENLIELHQINLGSHEGKSALRAVATPLIDKIPD 413 Query: 413 --------------SGLRLQIVRSVAQATQTTPA 432 +G Q+ R A+ T+ PA Sbjct: 414 PFLQEILEKLLDERTGFDHQLRRKKARKTENRPA 447