BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0029.0029.p (319 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2392 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Rso... 320 4e-87 PA1275 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Pae ... 295 1e-79 PH0376 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Pho ... 149 1e-35 PAB0025 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Pab... 145 1e-34 BH1588 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Bha ... 145 2e-34 >RSc2392 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Rso Ralstonia solanacearum Length = 331 Score = 320 bits (819), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 176/289 (60%), Positives = 204/289 (70%), Gaps = 7/289 (2%) Query: 17 GIALDLLLGEARRWHPLVGFGNLAKRIEGTLNHAPALRL----RGVLAWCLAVLPPVLAC 72 G+ LD LLGEA RWHPLVGFG +A IE LN PA R+ G++AWCLAVLP V Sbjct: 23 GVGLDRLLGEAPRWHPLVGFGRVAAWIERGLNRRPAARVLSRAAGMVAWCLAVLPGVAIA 82 Query: 73 AWIIAVLPGWL---AALAHAALLYFCIGLRSLREHTLPIAQALAQGDLPLARRLTSYIVS 129 AWI A+ P A +A LYF +G RSLR+H PIA+AL GDLP AR L S IVS Sbjct: 83 AWITALAPDTARLGAWIADVVALYFAVGARSLRDHIAPIAEALQAGDLPRARGLASRIVS 142 Query: 130 RDTRESGEAELAKAGVESLLENGNDAVFGTLFWFLVAGGPGALLFRLANTLDAMWGYRNA 189 RD ++ E +A+A VES LENG+DA+F LFW +V G PG +L+RLANTLDAMWGYRNA Sbjct: 143 RDLHDADEEAIARAAVESALENGSDAIFAPLFWLVVGGAPGVVLYRLANTLDAMWGYRNA 202 Query: 190 RFLFFGWSAARIDDGLNWLPARLTAFSYAWLGQRRQALDCWKAQAPAWSSPNAGPVMAAG 249 RF FGW+AARIDD LNW+PARLTA SYA LG AL CW+AQAP WSSPNAGPVMA+G Sbjct: 203 RFGRFGWAAARIDDVLNWMPARLTAASYALLGHTADALRCWRAQAPRWSSPNAGPVMASG 262 Query: 250 AGALGLALGGPAIYDGEVEERPPLGRGLPPQGPDIVRAWRLVAMSTALW 298 AG+L + LGG A YDG E RPPLG G P + DI RA LV+ + LW Sbjct: 263 AGSLRVQLGGMARYDGIDETRPPLGIGRPARAADIRRALALVSRTLGLW 311 >PA1275 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Pae Pseudomonas aeruginosa Length = 312 Score = 295 bits (754), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 173/290 (59%), Positives = 200/290 (68%), Gaps = 11/290 (3%) Query: 11 AALMALGIALDLLLGEARRWHPLVGFGNLAKRIEGTLNHA-------PAL--RLRGVLAW 61 A L LG+ LD GE + WHPLV FG LA R+E N + P + R GV AW Sbjct: 3 ALLGILGVVLDARFGEPQGWHPLVAFGRLADRLERRFNRSRLAGEADPGVGWRSHGVTAW 62 Query: 62 CLAVLPPVLACAWIIAVLPGWLAALAHAALLYFCIGLRSLREHTLPIAQALAQGDLPLAR 121 LAVLP L AW +A LP +L L A LY +GLRSL EH P+A AL GDL AR Sbjct: 63 VLAVLPLTL-LAWALAELP-YLGWLVQALALYAALGLRSLGEHAEPVALALRAGDLDEAR 120 Query: 122 RLTSYIVSRDTRESGEAELAKAGVESLLENGNDAVFGTLFWFLVAGGPGALLFRLANTLD 181 R Y+VSRDTRE +A +A+A ES+LENG+DAV+ LFWF VAG PG +L+RL+NTLD Sbjct: 121 RRVGYLVSRDTRELDQAGVARAATESVLENGSDAVYAALFWFAVAGAPGVVLYRLSNTLD 180 Query: 182 AMWGYRNARFLFFGWSAARIDDGLNWLPARLTAFSYAWLGQRRQALDCWKAQAPAWSSPN 241 AMWGYRNARF FGW+AA+IDD LN+LPARL A +YA LG RQAL CW+ QAP W SPN Sbjct: 181 AMWGYRNARFERFGWAAAKIDDILNYLPARLVALTYALLGDTRQALRCWREQAPQWDSPN 240 Query: 242 AGPVMAAGAGALGLALGGPAIYDGEVEERPPLGRGLPPQGPDIVRAWRLV 291 AGPVMAAGAGALG+ LGG AIY GE+ +RP LG G PQG DI RA LV Sbjct: 241 AGPVMAAGAGALGVFLGGAAIYHGELHQRPLLGSGPQPQGRDIYRALALV 290 >PH0376 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Pho Pyrococcus horikoshii Length = 283 Score = 149 bits (375), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 107/285 (37%), Positives = 151/285 (52%), Gaps = 27/285 (9%) Query: 21 DLLLGEA-RRWHPLVGFGNLAKRIEGTLNH-APALRLRGVLAWCLAVLPPVLACAWIIAV 78 DLLLGE HP+V FG L I+ + +PA+ LA A L VL+ A+++++ Sbjct: 11 DLLLGEPPAVVHPVVWFGKLIAFIDSHYSRRSPAI---DFLAGLFATLV-VLSFAFLLSI 66 Query: 79 LPGW----LAALAHAALLYFCIGLRSLREHTLPIAQALAQGDLPLARRLTSYIVSRDTRE 134 LP + L L LL +RSL EH + + D+ R+ S IVSRDT + Sbjct: 67 LPLYAPYPLNYLLSVYLLKSSFAIRSLYEHV----RRTMKDDVEEMRKEVSMIVSRDTSK 122 Query: 135 SGEAELAKAGVESLLENGNDAVFGTLFWFLVAGGPGALLFRLANTLDAMWGYRNARFLFF 194 G L A +ESL EN ND+V LF++L+ G PGAL++R NTLDAM GYR +R+ +F Sbjct: 123 LGREHLISASIESLAENTNDSVVAPLFYYLLFGLPGALVYRAVNTLDAMVGYRTSRYEYF 182 Query: 195 GWSAARIDDGLNWLPARLTAFSYAWLGQRRQALDCWKAQAPAWSSPNAGPVMAAGAGALG 254 G +AR+DD LN+LPAR+T + L RR + A N+ +AA + LG Sbjct: 183 GKFSARLDDILNFLPARITVLLFLPLNPRR----VIRYYKMARFKVNSDKPIAAMSAVLG 238 Query: 255 LALGGPAIYDGEVEERPPLGRGLPPQGPDIVRAWRLVAMSTALWV 299 + L P IY R P G P+ DI RA ++ + + W+ Sbjct: 239 IWLEKPNIY------RFP---GRDPRMEDIERALKVYVIVVSEWI 274 >PAB0025 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Pab Pyrococcus abyssi Length = 290 Score = 145 bits (365), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 111/310 (35%), Positives = 165/310 (53%), Gaps = 33/310 (10%) Query: 13 LMALGIAL--DLLLGEA-RRWHPLVGFGNLAKRIEGTLNHAPALRLRGVLAWCLA--VLP 67 L+ LG+AL DLLLGE + HP+V FG +A G L++ R RG + + V Sbjct: 4 LILLGLALIWDLLLGEPPAKIHPVVWFGKIA----GFLDNR--WRRRGKIGFLAGAFVTF 57 Query: 68 PVLACAWIIAVLPGWLA----ALAHAALLYFCIGLRSLREHTLPIAQALAQGDLPLARRL 123 ++A A+ ++++P +L L LL +RSL EH +A+ + + D+ R+ Sbjct: 58 IIVALAFFLSLIPSYLTFPLDYLLAIYLLKSSFAIRSLYEH---VARTVTE-DIEEKRKT 113 Query: 124 TSYIVSRDTRESGEAELAKAGVESLLENGNDAVFGTLFWFLVAGGPGALLFRLANTLDAM 183 S IVSRD + A L A +ESL EN ND+V LF+F++ G PGA+++R NTLDAM Sbjct: 114 VSMIVSRDVKVLDLAHLNSAAIESLAENLNDSVVAPLFYFMLFGLPGAMVYRAVNTLDAM 173 Query: 184 WGYRNARFLFFGWSAARIDDGLNWLPARLTAFSYAWLGQRRQALDCWKAQAPAWSSPNAG 243 +GYR+ R+ +FG AR+DD LN++PARLT Y G + K A N+ Sbjct: 174 FGYRDERYEYFGKFPARLDDILNFIPARLTVLLYLPFGVK-----VLKYYKLARFKINSD 228 Query: 244 PVMAAGAGALGLALGGPAIYDGEVEERPPLGRGLPPQGPDIVRAWRLVAMSTALWVAVVC 303 +AA + LG+ L P Y R P G P+ DI RA + + A ++++V Sbjct: 229 KPIAAMSAVLGVWLEKPGAY------RFP---GREPRDEDIKRALDVYKLVVAEYLSIVF 279 Query: 304 ALAVLTLAVG 313 L V+ L + Sbjct: 280 VLKVVQLCLN 289 >BH1588 [H] COG1270 Cobalamin biosynthesis protein CobD/CbiB Bha Bacillus halodurans Length = 319 Score = 145 bits (365), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 100/268 (37%), Positives = 140/268 (52%), Gaps = 12/268 (4%) Query: 20 LDLLLGEARRW--HPLVGFGNLAKRIEGTLNHAPALRLRGVLAWCLAVLPPV---LACAW 74 LD LG+ + W HP+VG G L E N R +GVL +L LA W Sbjct: 15 LDKWLGDPK-WLPHPVVGMGKLITYFERRWNCGRWRREKGVLLLLTVLLIVTALSLALVW 73 Query: 75 IIAVLPGWLAALAHAALLYFCIGLRSLREHTLPIAQALAQGDLPLARRLTSYIVSRDTRE 134 + + L + A L+ I + L+E +A+ LA +L ARR SYIV RDT + Sbjct: 74 LSYQVHLLLGVIVEALLIASTIAAKGLKEAAEEVARPLATRNLLEARRKLSYIVGRDTDQ 133 Query: 135 SGEAELAKAGVESLLENGNDAVFGTLFWFLVAGGPGALLFRLANTLDAMWGYRNARFLFF 194 E E+A+ +E++ EN +D V LF+ L+ G P ALL+R NT D+M GY+N R+ F Sbjct: 134 LDEKEIARGAIETVAENTSDGVTAPLFYALIGGAPLALLYRATNTCDSMVGYKNERYRDF 193 Query: 195 GWSAARIDDGLNWLPARLTAFSYAWLGQRR------QALDCWKAQAPAWSSPNAGPVMAA 248 GW++A+ DD LNW+P+R+T L ++R Q+L +A SPN+G AA Sbjct: 194 GWASAKFDDVLNWVPSRITGVLMLLLHRKRRQAPLGQSLKMLAREAKKHPSPNSGWGEAA 253 Query: 249 GAGALGLALGGPAIYDGEVEERPPLGRG 276 A L + LGG Y G ER +G G Sbjct: 254 MALLLHVTLGGTNTYQGMTSERAKMGYG 281