BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0135.p (219 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ECs4754 [E] COG1280 Putative threonine efflux protein Ecs Escher... 162 6e-40 PA5249 [E] COG1280 Putative threonine efflux protein Pae Pseudom... 159 4e-39 VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio ... 155 5e-38 STM3960 [E] COG1280 Putative threonine efflux protein Sty Salmon... 145 9e-35 YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersin... 135 6e-32 >ECs4754 [E] COG1280 Putative threonine efflux protein Ecs Escherichia coli O157:H7 Length = 206 Score = 162 bits (409), Expect = 6e-40, Method: Compositional matrix adjust. Identities = 88/209 (42%), Positives = 130/209 (62%), Gaps = 11/209 (5%) Query: 1 MAYQTWFAFFLAACVIAISPGSGAVLSMSHGLNYGLRRTTATILGLEIALVVVLIIAGAG 60 M + WFA+ L + ++++SPGSGA+ +M+ LN+G R A+I GL+ L + +++ G G Sbjct: 1 MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIAGLQTGLAIHIVLVGVG 60 Query: 61 VGSLLVASETAFNVVKIAGALYLIYLGFSQWRARVPSEQEGEALDNANAVARAQRGQHWW 120 +G+L S AF V+K AGA YLI+LG QWRA A+D ++A Q +H + Sbjct: 61 LGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRA-------AGAID-LKSLASTQSRRHLF 112 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHERPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 QR + N TNPK IVF+ A+ PQFI ++P +Q +VL VT + VD++VM GYA A Sbjct: 113 QRAV---FVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDIIVMIGYATLA 169 Query: 181 SRLQRFFRSQKAIKAQNRFFGGVLMLVGA 209 R+ + + K +KA N+ FG + MLVGA Sbjct: 170 QRIALWIKGPKQMKALNKIFGSLFMLVGA 198 >PA5249 [E] COG1280 Putative threonine efflux protein Pae Pseudomonas aeruginosa Length = 209 Score = 159 bits (402), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 92/209 (44%), Positives = 122/209 (58%), Gaps = 12/209 (5%) Query: 1 MAYQTWFAFFLAACVIAISPGSGAVLSMSHGLNYGLRRTTATILGLEIALVVVLIIAGAG 60 M TWFAFFLA I++SPG+GA+ SMS GL YG R LGL+I L + + I AG Sbjct: 1 MLVSTWFAFFLACWAISLSPGAGAIASMSCGLQYGFARGYWNALGLQIGLALQIAIVAAG 60 Query: 61 VGSLLVASETAFNVVKIAGALYLIYLGFSQWRARVPSEQEGEALDNANAVARAQRGQHWW 120 VG+LL S AF+++K G YL+YL QW+A + + D + R Sbjct: 61 VGALLATSALAFSLIKWFGVAYLVYLAVRQWQA----PPQALSTDGERPLGRPL------ 110 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHERPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 + GFL NA+NPK ++FM+AVLPQFID +PL Q L++ TM+ VDL+VM GY A Sbjct: 111 TLVLRGFLVNASNPKAVIFMLAVLPQFIDPHQPLLAQYLIMGGTMIVVDLIVMAGYTGLA 170 Query: 181 SRLQRFFRSQKAIKAQNRFFGGVLMLVGA 209 +R+ R RS + K NR F + VGA Sbjct: 171 ARVLRVLRSPRQQKLVNRTFAS--LFVGA 197 >VC0136 [E] COG1280 Putative threonine efflux protein Vch Vibrio cholerae Length = 205 Score = 155 bits (393), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 84/215 (39%), Positives = 120/215 (55%), Gaps = 17/215 (7%) Query: 1 MAYQTWFAFFLAACVIAISPGSGAVLSMSHGLNYGLRRTTATILGLEIALVVVLIIAGAG 60 M W A+ L A V +++PGSG V S+S+GL+YG R + I+GL+I L +++ G G Sbjct: 1 MDIHVWLAYLLTAVVFSLAPGSGTVNSISNGLSYGTRHSLGAIIGLQIGLACHIVLVGIG 60 Query: 61 VGSLLVASETAFNVVKIAGALYLIYLGFSQWRARVP--SEQEGEALDNANAVARAQRGQH 118 +G+L+ S AF ++K GA YL++LG +WR R P + L A + +A Sbjct: 61 IGALVAQSALAFTLIKWIGAAYLVWLGIQKWRDRAPLTATTTSHELSQAALLRKA----- 115 Query: 119 WWQRCMTGFLTNATNPKGIVFMVAVLPQFIDHERPLWLQLLVLAVTMVAVDLVVMHGYAF 178 L N TNPK IVF+VA+ PQFID R W Q LVL +T V +D +VM GY Sbjct: 116 --------VLINLTNPKSIVFLVALFPQFIDPTRDHWPQFLVLGITTVTIDAIVMFGYTA 167 Query: 179 AASRLQRFFRSQKAIKAQNRFFGGVLMLVGAGLFF 213 A++L R+ RS + N+ FG M +G G+ Sbjct: 168 LAAQLGRYIRSPNIMTRMNKLFGS--MFMGCGMLL 200 >STM3960 [E] COG1280 Putative threonine efflux protein Sty Salmonella typhimurium LT2 Length = 206 Score = 145 bits (365), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 85/209 (40%), Positives = 129/209 (61%), Gaps = 11/209 (5%) Query: 1 MAYQTWFAFFLAACVIAISPGSGAVLSMSHGLNYGLRRTTATILGLEIALVVVLIIAGAG 60 M ++ WFA+ L + ++++SPGSGA+ +M+ +N+G R A+I GL+ L + +++ G G Sbjct: 1 MTFEWWFAYLLTSTLLSLSPGSGAINTMTTSINHGYRGAVASIAGLQTGLGIHIVLVGVG 60 Query: 61 VGSLLVASETAFNVVKIAGALYLIYLGFSQWRARVPSEQEGEALDNANAVARAQRGQHWW 120 +G+L S AF ++K AGA YLI+LG QWRA A+D + +A+ Q + Sbjct: 61 LGTLFSRSLLAFEILKWAGAAYLIWLGIQQWRA-------AGAID-LHTLAQTQSRGRLF 112 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHERPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 +R + N TNPK IVF+ A+ PQFI ++P Q L+L VT + VD+VVM GYA A Sbjct: 113 KRAI---FVNLTNPKSIVFLAALFPQFIMPQQPQLAQYLILGVTTIVVDMVVMTGYATLA 169 Query: 181 SRLQRFFRSQKAIKAQNRFFGGVLMLVGA 209 R+ + + K +KA N+ FG + MLVGA Sbjct: 170 QRIAAWIKGPKQMKALNKAFGSLFMLVGA 198 >YPO3831 [E] COG1280 Putative threonine efflux protein Ype Yersinia pestis Length = 206 Score = 135 bits (341), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 82/209 (39%), Positives = 128/209 (61%), Gaps = 11/209 (5%) Query: 1 MAYQTWFAFFLAACVIAISPGSGAVLSMSHGLNYGLRRTTATILGLEIALVVVLIIAGAG 60 M W + L ++++SPGSGA+ +MS +++G R A+I GL++ L V +++ G G Sbjct: 1 MTLDWWLTYLLTTLILSLSPGSGAINTMSTAISHGTRGVVASIGGLQLGLAVHIVLVGVG 60 Query: 61 VGSLLVASETAFNVVKIAGALYLIYLGFSQWRARVPSEQEGEALDNANAVARAQRGQHWW 120 +G+L+ S AF ++K GA YLI+LG QWRA +LD +A+A + + + Sbjct: 61 LGALVSQSLLAFEILKWLGAAYLIWLGIQQWRA-------AGSLD-LHALANSMPRRKLF 112 Query: 121 QRCMTGFLTNATNPKGIVFMVAVLPQFIDHERPLWLQLLVLAVTMVAVDLVVMHGYAFAA 180 +R + N TNPK IVF+ A+ PQF+ ++P Q L+L T V VD++VM GYA A Sbjct: 113 KRAV---FVNLTNPKSIVFLAALFPQFVLPQQPQVAQYLILGSTSVIVDIIVMIGYATLA 169 Query: 181 SRLQRFFRSQKAIKAQNRFFGGVLMLVGA 209 +R+ R+ +S + +K NR FGG+ ML+GA Sbjct: 170 TRIARWIKSPQQMKLLNRIFGGLFMLIGA 198