BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0098.p (1392 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC1451 [Q] COG2931 RTX toxins and related Ca2+-binding proteins ... 104 2e-21 RSp0295 [Q] COG2931 RTX toxins and related Ca2+-binding proteins... 82 1e-14 all2654_2 [Q] COG2931 RTX toxins and related Ca2+-binding protei... 63 4e-09 >VC1451 [Q] COG2931 RTX toxins and related Ca2+-binding proteins Vch Vibrio cholerae Length = 4558 Score = 104 bits (259), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 82/228 (35%), Positives = 116/228 (50%), Gaps = 13/228 (5%) Query: 861 QGEGDVAIVGGEDNRVMTGDGDDLALVLGKTNALFMGDGNDAGLMAGEKNAWRGGKGNDL 920 QG+GD+ I G N GDG DL LG N + G G+D G+ N + GG+GND+ Sbjct: 4190 QGDGDINISLGNYNFNWGGDGKDLGAYLGDNNNFWGGRGDDVFYATGKSNIFTGGEGNDM 4249 Query: 921 VLAAGKENGVKGNEGNDILFVLGQTNKVEGGSGDDAVLALGKSNDVDGGEGNDLMLIVGQ 980 + G+EN + G +GND V G+ N V G+GDD G+ ++D G G D ++ G Sbjct: 4250 GVLMGRENMMFGGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGN 4309 Query: 981 KNNVNGKAEDATGDGDDTLLVLGQENQVRGGRGNDWLIACGLSNEMDGGQGNDVLVAAGW 1040 N V+ TGD D + +G NQV G GND+ G N ++ G GNDV+ G+ Sbjct: 4310 FNRVD------TGDDQDYSVTIGNNNQVELGAGNDFANIFGNYNRINAGAGNDVVKLMGY 4363 Query: 1041 KNTLYGDFAGSDNGNDVMISLGGANKVQGNG--GNDLIVAAGLGNQLE 1086 L G +G+D +I+ + Q NG G DL+V G N + Sbjct: 4364 HAVLNG-----GDGDDHLIATAISKFSQFNGGEGRDLMVLGGYQNTFK 4406 Score = 102 bits (254), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 85/231 (36%), Positives = 121/231 (52%), Gaps = 18/231 (7%) Query: 919 DLVLAAGKENGVKGNEGNDILFVLGQTNKVEGGSGDDAVLALGKSNDVDGGEGNDLMLIV 978 D+ ++ G N G +G D+ LG N GG GDD A GKSN GGEGND+ +++ Sbjct: 4194 DINISLGNYNFNWGGDGKDLGAYLGDNNNFWGGRGDDVFYATGKSNIFTGGEGNDMGVLM 4253 Query: 979 GQKNNVNGKAEDATGDGDDTLLVLGQENQVRGGRGNDWLIACGLSNEMDGGQGNDVLVAA 1038 G++N + G GDG+DT +V G+ N V G G+D G E+D G G D +V + Sbjct: 4254 GRENMMFG------GDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTS 4307 Query: 1039 GWKNTLYGDFAGSDNGND--VMISLGGANKVQGNGGNDLIVAAGLGNQLEANAGNDIVLA 1096 G +F D G+D +++G N+V+ GND G N++ A AGND+V Sbjct: 4308 G-------NFNRVDTGDDQDYSVTIGNNNQVELGAGNDFANIFGNYNRINAGAGNDVVKL 4360 Query: 1097 AGLGQSIDTGEGDD--IAVALGAGNQISAGSGKDTLYVAGALGNVFAGSDD 1145 G ++ G+GDD IA A+ +Q + G G+D L V G N F G D Sbjct: 4361 MGYHAVLNGGDGDDHLIATAISKFSQFNGGEGRD-LMVLGGYQNTFKGGTD 4410 Score = 95.9 bits (237), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 74/207 (35%), Positives = 109/207 (52%), Gaps = 18/207 (8%) Query: 857 YRFNQGEGDVAIVG---GEDNRVMTGDGDDLALVLGKTNALFMGDGNDAGLMAGEKNAWR 913 Y FN G GD +G G++N G GDD+ GK+N G+GND G++ G +N Sbjct: 4202 YNFNWG-GDGKDLGAYLGDNNNFWGGRGDDVFYATGKSNIFTGGEGNDMGVLMGRENMMF 4260 Query: 914 GGKGNDLVLAAGKENGVKGNEGNDILFVLGQTNKVEGGSGDDAVLALGKSNDVDGGEGND 973 GG GND + AG+ N V G+D FV G+ +++ GSG D V+ G N VD G+ D Sbjct: 4261 GGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRVDTGDDQD 4320 Query: 974 LMLIVGQKNNVN-GKAED-----------ATGDGDDTLLVLGQENQVRGGRGNDWLIACG 1021 + +G N V G D G G+D + ++G + GG G+D LIA Sbjct: 4321 YSVTIGNNNQVELGAGNDFANIFGNYNRINAGAGNDVVKLMGYHAVLNGGDGDDHLIATA 4380 Query: 1022 LS--NEMDGGQGNDVLVAAGWKNTLYG 1046 +S ++ +GG+G D++V G++NT G Sbjct: 4381 ISKFSQFNGGEGRDLMVLGGYQNTFKG 4407 Score = 91.7 bits (226), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 122/375 (32%), Positives = 192/375 (51%), Gaps = 33/375 (8%) Query: 131 ALNVLSLVASAAFNA-----GNV-VVAGEGDNKVKAHGGYN-FVQTGSGHDAISMVGGFN 183 A+NVL + + A GN+ V G GD++V GGYN Q GSG+ GGFN Sbjct: 677 AVNVLVRLGDGQYLAHLLAYGNISVQKGSGDSRVVMLGGYNTHTQIGSGNGLWLAAGGFN 736 Query: 184 AAI-AGNGRNRIFAAGGNNIAVAGAGDDEMQA--LGAANVFFANAGDNRMT-----AIGT 235 G G AGG N+ + G+ E+ + LG ANV + D++++ A+G Sbjct: 737 VMTQVGKGDVAAVLAGGANV-LTKMGEGELTSGMLGGANVITHISNDDQLSNTTAVALGG 795 Query: 236 GNLFVAADGNDDMLAVGGGNIAIM---GGGNNTALLAGGYGNLVQGGAGDDRVLALSQWN 292 N+ G + LAV GG ++ G G T ++ GG L + G GD + L N Sbjct: 796 ANILTKK-GKGNTLAVMGGGANVLTHVGDGTTTGVMVGGANILTKVGNGDTTGILLGVGN 854 Query: 293 VMM-LGEGNNTVGALGGGGNLVWTGSGNDTVISLGFYVGNILL-LGDGDNFAISGGRANI 350 V+ +G+G T+G +G GN ++T G+ T I++ GNI +G+G+ +A+ GG N+ Sbjct: 855 VLTHVGDGQ-TLGVMGAAGN-IFTKVGDGTSIAVMIGAGNIFTHVGEGNAWALMGGLGNV 912 Query: 351 -VMAGSGRDSILSVGQTN-FVSTANGVDNVIVIGQYNLA-HTGGDDDIAAVIGKLN-FVD 406 G+G L V + N F +G+ +++ + N+A G +AA++G +N F Sbjct: 913 FTKVGNGDALALMVAEANVFTHIGDGMSVALMLAKGNVATKVGNGTTLAAMVGNVNIFTH 972 Query: 407 TGWGNDVALVIGKTNIVLTEAGDD--TALVVGRNNYV-FAGSGSDRVAVFGETNYVLA-G 462 G G+ A +IG+ NI +T+ G+D AL+VG+ N + G G+ GE N + G Sbjct: 973 IGHGSTFAAMIGQANI-MTKVGNDLTAALMVGKANIMTHVGDGTSLGLFAGEVNVMTKVG 1031 Query: 463 SGHDLVAVFGKRNVV 477 +G L A+FGK N++ Sbjct: 1032 NGTTLAAMFGKANIM 1046 Score = 76.3 bits (186), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 59/172 (34%), Positives = 87/172 (50%), Gaps = 6/172 (3%) Query: 308 GGGNLVWTGSGNDTVISLGFYVG--NILLLGDGDNFAISGGRANIVMAGSGRDSILSVGQ 365 G N W G G D LG Y+G N G GD+ + G++NI G G D + +G+ Sbjct: 4200 GNYNFNWGGDGKD----LGAYLGDNNNFWGGRGDDVFYATGKSNIFTGGEGNDMGVLMGR 4255 Query: 366 TNFVSTANGVDNVIVIGQYNLAHTGGDDDIAAVIGKLNFVDTGWGNDVALVIGKTNIVLT 425 N + +G D +V G+ N G DD + V G+ +DTG G D + G N V T Sbjct: 4256 ENMMFGGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRVDT 4315 Query: 426 EAGDDTALVVGRNNYVFAGSGSDRVAVFGETNYVLAGSGHDLVAVFGKRNVV 477 D ++ +G NN V G+G+D +FG N + AG+G+D+V + G V+ Sbjct: 4316 GDDQDYSVTIGNNNQVELGAGNDFANIFGNYNRINAGAGNDVVKLMGYHAVL 4367 Score = 74.3 bits (181), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 65/204 (31%), Positives = 94/204 (46%), Gaps = 29/204 (14%) Query: 278 GGAGDDRVLALSQWNVMMLGEGNNTVGALGGGGNLVWTGSGNDTVISLGFYVGNILLLGD 337 GG GDD A + N+ GEGN+ +G L G N+++ G GNDT Sbjct: 4225 GGRGDDVFYATGKSNIFTGGEGND-MGVLMGRENMMFGGDGNDT---------------- 4267 Query: 338 GDNFAISGGRANIVMAGSGRDSILSVGQTNFVSTANGVDNVIVIGQYNLAHTGGDDDIAA 397 A+ GR N V G+G D G+ + T +G D V+ G +N TG D D + Sbjct: 4268 ----AVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRVDTGDDQDYSV 4323 Query: 398 VIGKLNFVDTGWGNDVALVIGKTNIVLTEAGDDTALVVGRNNYVFAGSGSDR-----VAV 452 IG N V+ G GND A + G N + AG+D ++G + + G G D ++ Sbjct: 4324 TIGNNNQVELGAGNDFANIFGNYNRINAGAGNDVVKLMGYHAVLNGGDGDDHLIATAISK 4383 Query: 453 FGETNYVLAGSGHDLVAVFGKRNV 476 F + N G G DL+ + G +N Sbjct: 4384 FSQFN---GGEGRDLMVLGGYQNT 4404 Score = 69.3 bits (168), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 59/181 (32%), Positives = 86/181 (47%), Gaps = 3/181 (1%) Query: 286 LALSQWNVMMLGEGNNTVGALGGGGNLVWTGSGNDTVISLGFYVGNILLLGDGDNFAISG 345 ++L +N G+G + +GA G N W G G+D + G NI G+G++ + Sbjct: 4197 ISLGNYNFNWGGDGKD-LGAYLGDNNNFWGGRGDDVFYATG--KSNIFTGGEGNDMGVLM 4253 Query: 346 GRANIVMAGSGRDSILSVGQTNFVSTANGVDNVIVIGQYNLAHTGGDDDIAAVIGKLNFV 405 GR N++ G G D+ + G+ N V G D V G+ TG D G N V Sbjct: 4254 GRENMMFGGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRV 4313 Query: 406 DTGWGNDVALVIGKTNIVLTEAGDDTALVVGRNNYVFAGSGSDRVAVFGETNYVLAGSGH 465 DTG D ++ IG N V AG+D A + G N + AG+G+D V + G + G G Sbjct: 4314 DTGDDQDYSVTIGNNNQVELGAGNDFANIFGNYNRINAGAGNDVVKLMGYHAVLNGGDGD 4373 Query: 466 D 466 D Sbjct: 4374 D 4374 Score = 65.5 bits (158), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 46/119 (38%), Positives = 64/119 (53%) Query: 361 LSVGQTNFVSTANGVDNVIVIGQYNLAHTGGDDDIAAVIGKLNFVDTGWGNDVALVIGKT 420 +S+G NF +G D +G N G DD+ GK N G GND+ +++G+ Sbjct: 4197 ISLGNYNFNWGGDGKDLGAYLGDNNNFWGGRGDDVFYATGKSNIFTGGEGNDMGVLMGRE 4256 Query: 421 NIVLTEAGDDTALVVGRNNYVFAGSGSDRVAVFGETNYVLAGSGHDLVAVFGKRNVVAT 479 N++ G+DTA+V GR N+VF G+G D+ VFGE + GSG D V G N V T Sbjct: 4257 NMMFGGDGNDTAVVAGRINHVFLGAGDDQSFVFGEGGEIDTGSGRDYVVTSGNFNRVDT 4315 >RSp0295 [Q] COG2931 RTX toxins and related Ca2+-binding proteins Rso Ralstonia solanacearum Length = 692 Score = 81.6 bits (200), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 119/388 (30%), Positives = 180/388 (46%), Gaps = 44/388 (11%) Query: 18 GSNFLMNGNGRYLAHAYGGLNIVLSGRGPAEINAYGSTNIVMAGDGGNTLRAY---GIGY 74 G+N + +G+G H G N ++ G G ++A AGDG N +AY G Sbjct: 244 GNNTVSSGDGDVTVHVGAGNNDLILGNGHNVVDANRFLRGPAAGDGNN--QAYLGNGDNE 301 Query: 75 LT-GIGNLVVS-GSGDDRIKVGGLLAAVLSDFGGRDDEFAFDRIVVLGRDAEDTITKTAL 132 +T G GN +V G+GD+ I VG + + G + F D + +T Sbjct: 302 VTLGDGNNIVGVGTGDNTITVGNGSNTLYAGHGAGTNTITFG-------DGANLVT---- 350 Query: 133 NVLSLVASAAFNAGNVVVAGEGDNKVKAHGGYNFVQTGSGHDAISMVGGFNAAIAGNGRN 192 + N V G G++ + G N+V GSG D I + G N +AGNG N Sbjct: 351 ---------VGDGDNSVFGGNGNSTIWGGQGANYVTVGSGDDTIGLGNGDNTVMAGNGAN 401 Query: 193 RIFAAGGNNIAVAGAGDDEMQALGAANVFFANAGDNRMTA-IGTG-NLFVAADGNDDMLA 250 + G+N G GDD + N A GDN + A G G N A DG +++ Sbjct: 402 SVSVGNGDNQITLGGGDDTIGVGAGNNTIDAGGGDNTIYAGRGAGRNAITAGDGR-NVVT 460 Query: 251 VGGGNIAIMGGGNNTALLAGGYGNLVQGGAGDDRVLALSQWNVMMLGEGNNTVGALGGGG 310 VG G ++ G N+A+ G N + G GD+ V + NV+ G GNNTV +G G Sbjct: 461 VGDGASSVHLGNGNSAVYVGNGNNQIALGGGDNAVNGGNGTNVLAAGNGNNTV-WMGSGS 519 Query: 311 NLVWTGSGNDTVISLGFYVG--NILLLGDGDNFAISGGRANIVMAGSGRDSILSVGQTNF 368 NL+ G+GN++ I +G +G N + +G+G AN + G+G + I T+ Sbjct: 520 NLIVVGNGNNS-IGVGSAIGSSNTIQVGNG---------ANAIEVGAGTNDIYVGNGTDT 569 Query: 369 VSTANGVDNVIV-IGQYNLAHTGGDDDI 395 V T NG++++ + GQ L + GG D + Sbjct: 570 VKTGNGINSLHLGTGQVTLTNYGGQDTV 597 Score = 72.0 bits (175), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 109/396 (27%), Positives = 175/396 (44%), Gaps = 34/396 (8%) Query: 113 FDRIVVLGRDAEDTITKTALNVLSLVASAAF-------NAGNVVVAGEGDNKVKAHGGYN 165 D+IV+ G + + +A + ++ +A N N V +G+GD V G N Sbjct: 205 VDQIVLTGNREQHVVGNSADDSFAVNGAAGSYAELYLGNGNNTVSSGDGDVTVHVGAGNN 264 Query: 166 FVQTGSGHDAI---------SMVGGFNAAIAGNGRNRIFAAGGNNIAVAGAGDDEMQALG 216 + G+GH+ + + G N A GNG N + GNNI G GD+ + Sbjct: 265 DLILGNGHNVVDANRFLRGPAAGDGNNQAYLGNGDNEVTLGDGNNIVGVGTGDNTITVGN 324 Query: 217 AANVFFAN--AGDNRMTAIGTGNLFVAADGNDDMLAVGGGNIAIMGGGNNTALLAGGYGN 274 +N +A AG N +T NL DG++ + G GN I GG + G + Sbjct: 325 GSNTLYAGHGAGTNTITFGDGANLVTVGDGDNSVFG-GNGNSTIWGGQGANYVTVGSGDD 383 Query: 275 LVQGGAGDDRVLALSQWNVMMLGEGNNTVGALGGGGNLVWTGSGNDTVISLG----FYVG 330 + G GD+ V+A + N + +G G+N + LGGG + + G+GN+T+ + G Y G Sbjct: 384 TIGLGNGDNTVMAGNGANSVSVGNGDNQI-TLGGGDDTIGVGAGNNTIDAGGGDNTIYAG 442 Query: 331 -----NILLLGDGDNFAISGGRANIVMAGSGRDSILSVGQTNFVSTANGVDNVIVIGQ-Y 384 N + GDG N G A+ V G+G +S + VG N G DN + G Sbjct: 443 RGAGRNAITAGDGRNVVTVGDGASSVHLGNG-NSAVYVGNGNNQIALGGGDNAVNGGNGT 501 Query: 385 NLAHTGGDDDIAAVIGKLNFVDTGWGND---VALVIGKTNIVLTEAGDDTALVVGRNNYV 441 N+ G ++ + N + G GN+ V IG +N + G + V N + Sbjct: 502 NVLAAGNGNNTVWMGSGSNLIVVGNGNNSIGVGSAIGSSNTIQVGNGANAIEVGAGTNDI 561 Query: 442 FAGSGSDRVAVFGETNYVLAGSGHDLVAVFGKRNVV 477 + G+G+D V N + G+G + +G ++ V Sbjct: 562 YVGNGTDTVKTGNGINSLHLGTGQVTLTNYGGQDTV 597 >all2654_2 [Q] COG2931 RTX toxins and related Ca2+-binding proteins Nos Nostoc sp. PCC 7120 Length = 203 Score = 63.2 bits (152), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 65/190 (34%), Positives = 89/190 (46%), Gaps = 48/190 (25%) Query: 916 KGNDLVLAAGKENGVKGNEGNDILFVLGQTNKVEGGSGDDAVLALGKSNDVDGGEGNDLM 975 G+D ++ +E + GN GNDIL+ G +K+ GG+GDD +DGGEGND + Sbjct: 2 SGDDTLIGTDEEESLFGNGGNDILYARGGDDKLFGGAGDDL---------LDGGEGNDAL 52 Query: 976 LIVGQKNNVNGKAEDATGDGDDTLLVLGQENQVRGGRGNDWLIACGLSNEMDGGQGNDVL 1035 G G DTLL GG GND+L N +DGG GND+L Sbjct: 53 F---------------GGAGTDTLL---------GGAGNDYLTGGTGDNLLDGGDGNDLL 88 Query: 1036 VAAGWKNTLYGDFAGSDNGNDVMISLGGANKVQGNGGNDLIVAAGLGNQLEANAGNDIVL 1095 G ++TL G G+D++ S G + + G GND+I N G D ++ Sbjct: 89 YGNGGQDTLLG-----GAGDDIIYSGSGDDLINGGLGNDIIF---------LNGGQDTIV 134 Query: 1096 AAGLGQSIDT 1105 A G IDT Sbjct: 135 VA-QGAGIDT 143