BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0095.p (213 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH1969 [R] COG2872 Predicted metal-dependent hydrolases related ... 72 7e-13 PAB0066 [R] COG2872 Predicted metal-dependent hydrolases related... 72 9e-13 PH0108 [R] COG2872 Predicted metal-dependent hydrolases related ... 72 1e-12 TM1726 [R] COG2872 Predicted metal-dependent hydrolases related ... 69 7e-12 CAC0906 [R] COG2872 Predicted metal-dependent hydrolases related... 69 7e-12 >PH1969 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Pho Pyrococcus horikoshii Length = 404 Score = 72.4 bits (176), Expect = 7e-13, Method: Compositional matrix adjust. Identities = 65/236 (27%), Positives = 102/236 (43%), Gaps = 34/236 (14%) Query: 1 MTERLYLTDLALRA-DIHVQDCTPQDDGRYAVRLSATPFHPQGGGQPSDTGRI--GDVEV 57 M ER+Y D LR D + D + D R V L T F+P+GGGQP D G I D E+ Sbjct: 1 MVERVYYKDPYLREIDAKIVDVRKEKD-RVEVVLDRTIFYPEGGGQPGDRGVIKGNDFEI 59 Query: 58 L--RVVSESEGIVHYTA----APVAPGPAVAAVDAEPRQLHTRLHSAGHVIGQVMAELGW 111 + + ++ I H P +D E R + ++H+ H++ V+ +L Sbjct: 60 MVEDTIEKNGEIFHIGKLRGEIPKEGEKVKLYLDWEWRYGNMQMHTGQHILSAVLKKLYD 119 Query: 112 TPTKAHHWPGEARVVGVGAAADGTAQELVPAELERRCNELLAQDLPCVM----------- 160 T + + + V + E AELE N+++ DLP V+ Sbjct: 120 LDTTGFNIFRDYAKIEVNGEVNWDMIE--RAELE--VNKIIINDLPVVIEEYDKLPEEIA 175 Query: 161 --------AMDNGLRQIGFGELPAYPCGGTHVATLGEIGQIRIELVRIKKGEALVR 208 + +R + G++ PCGGTHV + E+G I++ L KK + L R Sbjct: 176 LKLRKNVTKVKEKIRIVKIGDVDVTPCGGTHVKSTREVGIIKV-LRFYKKSKNLWR 230 >PAB0066 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Pab Pyrococcus abyssi Length = 213 Score = 72.0 bits (175), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 61/210 (29%), Positives = 93/210 (44%), Gaps = 32/210 (15%) Query: 1 MTERLYLTDLALRADIHVQDCTPQDDGRYAVRLSATPFHPQGGGQPSDTGRIGDVEVLRV 60 MT +LY D L+ + + +D+ A+ L T F+P GGGQP D G I VEVL V Sbjct: 1 MTRKLYYEDAYLK-EAKAKVVEIKDN---ALLLDQTIFYPTGGGQPHDRGTINGVEVLDV 56 Query: 61 VSESEGIV-HYTAAP--VAPGPAVA-AVDAEPR----QLHTRLHSAGHVIGQVMAELGWT 112 + EG V H P G V +D + R ++HT LH HV+ +V+ E Sbjct: 57 YKDEEGNVWHVVKEPEKFKVGDEVELKIDWDYRYKLMRIHTGLHLLEHVLNEVLGE---- 112 Query: 113 PTKAHHWPGEARVVGVGAAADGTAQEL-VPAELERRCNELLAQDLPCV-------MAMDN 164 G+ +VG G + + ++ P L + ++++ V + + Sbjct: 113 --------GKWELVGSGMSVEKGRYDIAYPENLNKYKEQIISLFNKYVDEGGEVKIWWEG 164 Query: 165 GLRQIGFGELPAYPCGGTHVATLGEIGQIR 194 R + PCGGTHV + EIG I+ Sbjct: 165 ETRYTQIRDFEVIPCGGTHVKDIKEIGHIK 194 >PH0108 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Pho Pyrococcus horikoshii Length = 216 Score = 72.0 bits (175), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 61/214 (28%), Positives = 92/214 (42%), Gaps = 40/214 (18%) Query: 1 MTERLYLTDLALRAD----IHVQDCTPQDDGRYAVRLSATPFHPQGGGQPSDTGRIGDVE 56 MT +LY D L+ + ++D A+ L T F+P GGGQP D G I VE Sbjct: 4 MTRKLYYEDAYLKEAKGRVLEIRDN--------AILLDQTIFYPTGGGQPHDRGTINGVE 55 Query: 57 VLRVVSESEGIV-HYTAAP--VAPGPAVA-AVDAEPR----QLHTRLHSAGHVIGQVMAE 108 VL V + EG V H P G V +D + R ++HT LH HV+ +V+ E Sbjct: 56 VLDVYKDEEGNVWHVVKEPEKFKVGDEVELKIDWDYRYKLMRIHTGLHLLEHVLNEVLGE 115 Query: 109 LGWTPTKAHHWPGEARVVGVGAAADGTAQEL-VPAELERRCNELLAQDLPCV-------M 160 W ++VG G + + ++ P L + ++++ V + Sbjct: 116 GNW------------QLVGSGMSVEKGRYDIAYPENLNKYKEQIISLFNKYVDEGGEVKI 163 Query: 161 AMDNGLRQIGFGELPAYPCGGTHVATLGEIGQIR 194 + R + PCGGTHV + EIG I+ Sbjct: 164 WWEGDRRYTQIRDFEVIPCGGTHVKDIKEIGHIK 197 >TM1726 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Tma Thermotoga maritima Length = 373 Score = 68.9 bits (167), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 55/189 (29%), Positives = 86/189 (45%), Gaps = 22/189 (11%) Query: 26 DGRYAVRLSAT-PFHPQG-GGQPSDTGRIGDVEVLRVVSESEGIVHYTAAPVAPGPAVAA 83 DG + +S T PF+P G GGQ D G+IG VL+V ++ I+HY A + PG Sbjct: 14 DGEKIIAVSRTSPFYPDGKGGQLGDRGKIGPANVLKVKEKNGYILHYLDAHLEPGDYECE 73 Query: 84 VDAEPRQLHTRLHSAGHVIGQVMAELGWTPTKAHHWPGEARVVGVGA---------AADG 134 +D R+ H+A H++ ++ T + H + + A A+ Sbjct: 74 IDPARRKDIACQHTAQHILSAAFLKVADLETVSFHMGERVSTIDLNAPFVLEDVLEEAED 133 Query: 135 TAQELVPA-------ELERRCNELLA-QDLPCVMAMDNGLRQIGFGELPAYPCGGTHVAT 186 A E+V + E+E+ E + + LP V + +R + G+ CGG HV Sbjct: 134 LANEVVRSCERVEILEIEKEEAEKMNLRKLPNV---EGKVRVVKIGDFDVTACGGFHVEN 190 Query: 187 LGEIGQIRI 195 GEIG I+I Sbjct: 191 TGEIGLIKI 199 >CAC0906 [R] COG2872 Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain Cac Clostridium acetobutylicum Length = 387 Score = 68.9 bits (167), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 54/223 (24%), Positives = 97/223 (43%), Gaps = 18/223 (8%) Query: 1 MTERLYLTD-LALRADIHVQDCTPQDDGRYAVRLSATPFHPQGGGQPSDTGRIGDVEVLR 59 MT +L+ D ++ + D ++DG+Y + L T F+P+GGGQP D G I ++ V Sbjct: 1 MTRKLFYDDPYICESEGKIVDVL-ENDGKYEIVLDETSFYPEGGGQPCDLGTINNIPVEY 59 Query: 60 VVSESEGIVHYTAAPVAPGPAVAAVDAEPRQLHTRLHSAGHVIGQVMAELGWTPTKAHHW 119 V + + I H A VD RQ + + H+ H++ +L H Sbjct: 60 VYEKGDTIYHVMKERPEGNTAKCKVDFLRRQDNIQQHTGEHLLSAAFFKLYNGVNYGFHM 119 Query: 120 PGEARVVGVG---------AAADGTAQELVPAELERRCNELL---AQDLPCVMAM--DNG 165 + + + + A + A +E + + A+ LP A+ + Sbjct: 120 GEDYVTIDIDMEEVTNDMIKKIEAEANAYIYANVETKTYFMSKPEAEKLPLRKAIKAEGK 179 Query: 166 LRQIGFGELPAYPCGGTHVATLGEIGQIRIELVRIKKGEALVR 208 +R + G++ C GTHV GE+G I++ +R +K + + R Sbjct: 180 IRIVQLGDVDYSACCGTHVVRTGEVGIIKV--LRTEKYKGMTR 220