BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0028.0021.p (686 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value maoC_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Eco Esc... 513 e-145 SMb21635_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Sme... 513 e-145 DR2381_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Dra D... 474 e-133 DR2381_2 [I] COG2030 Acyl dehydratase Dra Deinococcus radiodurans 267 5e-71 maoC_2 [I] COG2030 Acyl dehydratase Eco Escherichia coli K12 234 6e-61 >maoC_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Eco Escherichia coli K12 Length = 500 Score = 513 bits (1322), Expect = e-145, Method: Compositional matrix adjust. Identities = 285/503 (56%), Positives = 347/503 (68%), Gaps = 8/503 (1%) Query: 8 LQSFIAGRW-FGESPAQALHSAVNGHTVASTHQESIDFGEALEHARKKGLPALMQLDFQQ 66 L SF++G W G ++ +H A++G + E +D A + A +KG PAL + F + Sbjct: 4 LASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAARQFAIEKGAPALRAMTFIE 63 Query: 67 RAAILKALAKYLVEHKETLYAVSAHTGATRTDSWIDIEGGSGTLFTYASIGTNEFPSSNL 126 RAA+LKA+AK+L+ KE YA+SA TGATR DSW+DIEGG GTLFTYAS+G+ E P L Sbjct: 64 RAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEGGIGTLFTYASLGSRELPDDTL 123 Query: 127 VHEGPAISLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMPCIVKPA 186 E I L K+G FA H+L + GVAVHINAFNFP WGMLEK APT+L GMP I+KPA Sbjct: 124 WPEDELIPLSKEGGFAARHLLTSKSGVAVHINAFNFPCWGMLEKLAPTWLGGMPAIIKPA 183 Query: 187 TATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLDRLQAQDVVTFTGSADTAAKLRAH 246 TAT+ L +A V+ I SGL+PEG++ L+ GS GDLLD L +QDVVTFTGSA T LR Sbjct: 184 TATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDLLDHLDSQDVVTFTGSAATGQMLRVQ 243 Query: 247 PNLIANSIPFNAEADSLNCAILGPDVNADDEEFDLFVKEVAREMTTKAGQKCTAIRRAIV 306 PN++A SIPF EADSLNC +LG DV D EF LF++EV REMTTKAGQKCTAIRR IV Sbjct: 244 PNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEFALFIREVVREMTTKAGQKCTAIRRIIV 303 Query: 307 PRQHLDAISEKLAARLSKTVIGDPALESVRMGPLASRAQQRDVEERV-ALLKQSAELILG 365 P+ ++A+S+ L ARL K V+GDPA E V+MG L + Q+ DV+E+V LL E+ LG Sbjct: 304 PQALVNAVSDALVARLQKVVVGDPAQEGVKMGALVNAEQRADVQEKVNILLAAGCEIRLG 363 Query: 366 AGSSFQPQGEGVGEGAFFAPTLLLARDTSAGSPVHEVEAFGPVSTLMPYDDLDEALALAA 425 Q + GAFF PTLL VH EAFGPV+TLMP + AL LA Sbjct: 364 G------QADLSAAGAFFPPTLLYCPQPDETPAVHATEAFGPVATLMPAQNQRHALQLAC 417 Query: 426 RGRGSLVASLVTRSPSVAARAIPVAAAWHGRLLVLDREAAPESTGHGSPLPQLKHGGPGR 485 G GSL +LVT P +A + I AA HGR+ +L+ E+A ESTGHGSPLPQL HGGPGR Sbjct: 418 AGGGSLAGTLVTADPQIARQFIADAARTHGRIQILNEESAKESTGHGSPLPQLVHGGPGR 477 Query: 486 AGGGEELGGSRAVKHYLQRTAVQ 508 AGGGEELGG RAVKHY+QRTAVQ Sbjct: 478 AGGGEELGGLRAVKHYMQRTAVQ 500 >SMb21635_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Sme Sinorhizobium meliloti Length = 487 Score = 513 bits (1320), Expect = e-145, Method: Compositional matrix adjust. Identities = 276/485 (56%), Positives = 332/485 (68%) Query: 18 GESPAQALHSAVNGHTVASTHQESIDFGEALEHARKKGLPALMQLDFQQRAAILKALAKY 77 G L A G VA IDF AL + R+KG PAL ++ F +RA +LKALA+ Sbjct: 3 GSKDGVTLCDAATGAPVALVDSSGIDFAAALAYGREKGGPALRRMSFHERAMMLKALAQA 62 Query: 78 LVEHKETLYAVSAHTGATRTDSWIDIEGGSGTLFTYASIGTNEFPSSNLVHEGPAISLGK 137 L+E KE YA+S TGATR DSWIDIEGG GTLF+YAS G E P+S+++ +G +L + Sbjct: 63 LMERKEEFYALSTATGATRADSWIDIEGGIGTLFSYASKGRRELPNSHVLLDGDVEALSR 122 Query: 138 QGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMPCIVKPATATSYLAEAAV 197 G F+ HIL P G+AVHINAFNFP WGMLEK APT LAGMP IVKPA+ T+YLAE V Sbjct: 123 DGTFSAQHILTPLQGIAVHINAFNFPCWGMLEKLAPTLLAGMPAIVKPASQTAYLAELVV 182 Query: 198 RLIQQSGLLPEGSLQLVIGSTGDLLDRLQAQDVVTFTGSADTAAKLRAHPNLIANSIPFN 257 R I +GLLPEG+LQLV GS GDLLDR+ QDVVTFTGSA T KL+ HP ++ NS+ F Sbjct: 183 RRIVDTGLLPEGALQLVCGSPGDLLDRVGDQDVVTFTGSATTGRKLKTHPAIVGNSVRFT 242 Query: 258 AEADSLNCAILGPDVNADDEEFDLFVKEVAREMTTKAGQKCTAIRRAIVPRQHLDAISEK 317 EADSLN A+LG D EEF LFV+EVAREMT+KAGQKCTAIRR I PR H DA+ Sbjct: 243 MEADSLNAAVLGLDAAPGAEEFGLFVREVAREMTSKAGQKCTAIRRVIAPRAHCDALITA 302 Query: 318 LAARLSKTVIGDPALESVRMGPLASRAQQRDVEERVALLKQSAELILGAGSSFQPQGEGV 377 L RL+K +GDPA E+VRMGPLASR Q+ +V R+ L AE++ G + Q Sbjct: 303 LGERLAKVPLGDPADENVRMGPLASRDQREEVRARILDLTTDAEIVAGDPARPQLVSGDA 362 Query: 378 GEGAFFAPTLLLARDTSAGSPVHEVEAFGPVSTLMPYDDLDEALALAARGRGSLVASLVT 437 GAF P LL A VH+VEAFGPVST+MPYD +EA+ L RGRGSLV S+ T Sbjct: 363 EAGAFLNPVLLYCDSPDAARSVHDVEAFGPVSTVMPYDTAEEAVDLVRRGRGSLVTSVFT 422 Query: 438 RSPSVAARAIPVAAAWHGRLLVLDREAAPESTGHGSPLPQLKHGGPGRAGGGEELGGSRA 497 P +A + A +HGR+++ +R +A STGHGSPLP L HGGPGRAGGGEELGG R Sbjct: 423 NDPDIARELVIGMAPFHGRVMIGNRLSAKSSTGHGSPLPGLVHGGPGRAGGGEELGGMRG 482 Query: 498 VKHYL 502 V+HY+ Sbjct: 483 VRHYM 487 >DR2381_1 [C] COG1012 NAD-dependent aldehyde dehydrogenases Dra Deinococcus radiodurans Length = 473 Score = 474 bits (1221), Expect = e-133, Method: Compositional matrix adjust. Identities = 254/463 (54%), Positives = 309/463 (66%), Gaps = 2/463 (0%) Query: 10 SFIAGRWFGESPAQALHSAVNGHTVASTHQESIDFGEALEHARKKGLPALMQLDFQQRAA 69 S++ G+W Q L AV G VA E +DF +AL + R+ G L +L F RA Sbjct: 13 SYVYGQWHANKDGQTLLDAVYGRPVAVISSEGVDFAQALAYGREAG-AGLRKLTFHTRAR 71 Query: 70 ILKALAKYLVEHKETLYAVSAHTGATRTDSWIDIEGGSGTLFTYASIGTNEFPSSNLVHE 129 +LKAL YL+E KE YA++ TGATR D W+DIEGG GTLF+Y+S+ E P + + Sbjct: 72 MLKALGNYLMERKEDYYALNLLTGATRRDGWVDIEGGIGTLFSYSSMARRELPDERFLPD 131 Query: 130 GPAISLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMPCIVKPATAT 189 G LGK G F H+LVPR GVAV INA+NFPVWGMLEK AP F+AGMP +VKPA T Sbjct: 132 GKVEVLGKGGTFMARHLLVPREGVAVQINAYNFPVWGMLEKLAPAFIAGMPSLVKPAPQT 191 Query: 190 SYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLDRLQAQDVVTFTGSADTAAKLRAHPNL 249 +YL E VR I SG+LPEGSLQLV G GDLLD L QD+V FTGSA TA KL+ HPN+ Sbjct: 192 AYLTERVVRDIIASGILPEGSLQLVTGDPGDLLDHLLEQDMVAFTGSAATANKLKVHPNI 251 Query: 250 IANSIPFNAEADSLNCAILGPDVNADDEEFDLFVKEVAREMTTKAGQKCTAIRRAIVPRQ 309 +A SIPFNAEADSLN A+LG V D EF LFVKEVAREMT KAGQKCTAIRR +VP Sbjct: 252 VARSIPFNAEADSLNAAVLGVTVRPKDPEFALFVKEVAREMTGKAGQKCTAIRRPLVPCH 311 Query: 310 HLDAISEKLAARLSKTVIGDPALESVRMGPLASRAQQRDVEERVALLKQSAELILGAGSS 369 +D + E L A L+K +GDPA + VRMG L S Q+ V E + L Q AE+++G G+ Sbjct: 312 LVDPVVEALRAELAKVTVGDPARDDVRMGALVSVEQRERVRETLEKLGQDAEVVIGGGAG 371 Query: 370 FQPQGEGVGEGAFFAPTLLLARDTSAGSPVHEVEAFGPVSTLMPYDDLDEALALAARGRG 429 G+ +GAF PT+LL R+ HE+EAFGPV+TLMPYD LD+A+ LA GRG Sbjct: 372 ELLGGDR-EKGAFLDPTVLLCRNPLEAKGPHELEAFGPVATLMPYDTLDDAITLAKMGRG 430 Query: 430 SLVASLVTRSPSVAARAIPVAAAWHGRLLVLDREAAPESTGHG 472 SL AS+VT + A + A+ HGR+LVL+R A ESTGHG Sbjct: 431 SLAASVVTFNRHEATELVLGIASTHGRVLVLNRADAKESTGHG 473 >DR2381_2 [I] COG2030 Acyl dehydratase Dra Deinococcus radiodurans Length = 227 Score = 267 bits (683), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 136/210 (64%), Positives = 163/210 (77%) Query: 473 SPLPQLKHGGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGAKVRESDI 532 SPLPQL HGGPGRAGGG E+ G VKH++ + AVQ PT LAA+ EH+ GA+VRE + Sbjct: 1 SPLPQLLHGGPGRAGGGAEMAGLAGVKHHMNKVAVQADPTTLAAITKEHIPGAEVREDVV 60 Query: 533 HPFRHYFEDLQIGDSLATHRRTVTEADIVNFGCLSGDHFYMHFDDISARQSPFGKRIAHG 592 HPFR F+ +Q GDSL THRRTVTEADIVNF L+GDHFY H DDI A++ FGKR+AHG Sbjct: 61 HPFRKTFDQIQPGDSLLTHRRTVTEADIVNFAALTGDHFYAHVDDIGAKEGIFGKRVAHG 120 Query: 593 YFVLSAAAGLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQ 652 YF++SAAAG FVS APGPVLANYGL+ LRFV+PVGIGDTI RLTCKRKI ++ + + Sbjct: 121 YFLISAAAGQFVSAAPGPVLANYGLENLRFVEPVGIGDTIRTRLTCKRKIRKDLRPGEER 180 Query: 653 GQGVVAWDVEVSNQDGELVASYDILTLVAK 682 GVV W E++NQ +LVA+YDILTLV + Sbjct: 181 PTGVVEWHAEITNQHEQLVATYDILTLVER 210 >maoC_2 [I] COG2030 Acyl dehydratase Eco Escherichia coli K12 Length = 181 Score = 234 bits (596), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 114/175 (65%), Positives = 133/175 (76%) Query: 509 GSPTMLAAVVGEHVRGAKVRESDIHPFRHYFEDLQIGDSLATHRRTVTEADIVNFGCLSG 568 GSPTMLAA+ + VRGAKV E IHPFR YFE+LQ GDSL T RRT+TEADIVNF CLSG Sbjct: 1 GSPTMLAAISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSG 60 Query: 569 DHFYMHFDDISARQSPFGKRIAHGYFVLSAAAGLFVSPAPGPVLANYGLDTLRFVKPVGI 628 DHFY H D I+A +S FG+R+ HGYFVLSAAAGLFV GPV+ANYGL++LRF++PV Sbjct: 61 DHFYAHMDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKP 120 Query: 629 GDTISARLTCKRKIDRNKKDANGQGQGVVAWDVEVSNQDGELVASYDILTLVAKK 683 GDTI RLTCKRK + ++ A + GVV W VEV NQ VA Y ILTLVA++ Sbjct: 121 GDTIQVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQ 175