BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0058.p (293 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc3355 [E] COG3186 Phenylalanine-4-hydroxylase Rso Ralstonia so... 316 4e-86 CC1612 [E] COG3186 Phenylalanine-4-hydroxylase Ccr Caulobacter v... 283 3e-76 mlr4831 [E] COG3186 Phenylalanine-4-hydroxylase Mlo Mesorhizobiu... 242 6e-64 PA0872 [E] COG3186 Phenylalanine-4-hydroxylase Pae Pseudomonas a... 200 3e-51 VCA0828 [E] COG3186 Phenylalanine-4-hydroxylase Vch Vibrio cholerae 191 2e-48 >RSc3355 [E] COG3186 Phenylalanine-4-hydroxylase Rso Ralstonia solanacearum Length = 313 Score = 316 bits (810), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 158/285 (55%), Positives = 191/285 (67%), Gaps = 8/285 (2%) Query: 3 TNPNTDDFFATVATKSDSGALRGDYSQADANYVVAQNWEGYTPAQHALWRRLYERQAKLI 62 T TD A D LR D++ + Q YT A HA WR LY+RQ L+ Sbjct: 19 TGTLTDKLREQFAEGLDGQTLRPDFT-------MEQPVHRYTAADHATWRTLYDRQEALL 71 Query: 63 PGRACDVFIDSLKALDVS-QGIPRFDRTTEALFKATGWQLVAVPGLVPDHTFFEHLANRR 121 PGRACD F+ L L +S +G+P FDR E L +ATGWQ+VAVPGLVPD FFEHLANRR Sbjct: 72 PGRACDEFLQGLSTLGMSREGVPSFDRLNETLMRATGWQIVAVPGLVPDEVFFEHLANRR 131 Query: 122 FPVTVWLREEHEFDYIVEPDLFHDFFGHVPLLFNPVFANHLQEYGKGGLKALKLDGLAYL 181 FP + W+R + DY+ EPD FHD FGHVPLL NPVFA+++Q YG+GGLKA +L L L Sbjct: 132 FPASWWMRRPDQLDYLQEPDGFHDIFGHVPLLINPVFADYMQAYGQGGLKAARLGALDML 191 Query: 182 ARLYWYTIEFGLIQSKEGLRIYGAGILSSGGEVEYCLSSDQPRRVPFEVERIMRTLYKID 241 ARLYWYT+EFGLI++ GLRIYGAGI+SS E Y L S P R+ F+V RIMRT Y+ID Sbjct: 192 ARLYWYTVEFGLIRTPAGLRIYGAGIVSSKSESVYALDSASPNRIGFDVHRIMRTRYRID 251 Query: 242 TYQETYFVIKDFEQLFNDTAPDFTPLYARLKQQEALPANTLLPGE 286 T+Q+TYFVI FEQLF+ T PDFTPLY L A ++ G+ Sbjct: 252 TFQKTYFVIDSFEQLFDATRPDFTPLYEALGTLPTFGAGDVVDGD 296 >CC1612 [E] COG3186 Phenylalanine-4-hydroxylase Ccr Caulobacter vibrioides Length = 294 Score = 283 bits (724), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 138/271 (50%), Positives = 181/271 (66%), Gaps = 1/271 (0%) Query: 18 SDSGALRGDYSQADANYVVAQNWEGYTPAQHALWRRLYERQAKLIPGRACDVFIDSLKAL 77 S G G A ++ + Q WE YT A+H +W LYERQ ++ GRACD F+ L AL Sbjct: 2 SGDGLSNGPPPGARPDWTIDQGWETYTQAEHDVWITLYERQTDMLHGRACDEFMRGLDAL 61 Query: 78 DVSQ-GIPRFDRTTEALFKATGWQLVAVPGLVPDHTFFEHLANRRFPVTVWLREEHEFDY 136 D+ + GIP F R E L + TGW +VAVPGLVPD FF+HLANRRFP ++R+ HE DY Sbjct: 62 DLHRSGIPDFARINEELKRLTGWTVVAVPGLVPDDVFFDHLANRRFPAGQFIRKPHELDY 121 Query: 137 IVEPDLFHDFFGHVPLLFNPVFANHLQEYGKGGLKALKLDGLAYLARLYWYTIEFGLIQS 196 + EPD+FHD FGHVP+L +PVFA+++Q YG+GG +AL L LA LARLYWYT+EFGL+ + Sbjct: 122 LQEPDIFHDVFGHVPMLTDPVFADYMQAYGEGGRRALGLGRLANLARLYWYTVEFGLMNT 181 Query: 197 KEGLRIYGAGILSSGGEVEYCLSSDQPRRVPFEVERIMRTLYKIDTYQETYFVIKDFEQL 256 GLRIYGAGI+SS E + L P R+ F++ER+MRTLY+ID +Q+ YFVI + L Sbjct: 182 PAGLRIYGAGIVSSRTESIFALDDPSPNRIGFDLERVMRTLYRIDDFQQVYFVIDSIQTL 241 Query: 257 FNDTAPDFTPLYARLKQQEALPANTLLPGET 287 T DF +Y RL + ++PG+ Sbjct: 242 QEVTLRDFGAIYERLASVSDIGVAEIVPGDA 272 >mlr4831 [E] COG3186 Phenylalanine-4-hydroxylase Mlo Mesorhizobium loti Length = 275 Score = 242 bits (618), Expect = 6e-64, Method: Compositional matrix adjust. Identities = 123/273 (45%), Positives = 174/273 (63%), Gaps = 3/273 (1%) Query: 12 ATVATKSDSGALRGDYSQADANYVVAQNWEGYTPAQHALWRRLYERQAKLIPGRACDVFI 71 A A + LRGDYS A++ VAQ+++ Y+ + A+WR L +RQ KL A ++ Sbjct: 4 AEYARDCAAQGLRGDYSVCRADFTVAQDYD-YSDEEQAVWRTLCDRQTKLTRKLAHHSYL 62 Query: 72 DSLKALDVSQGIPRFDRTTEALFKATGWQLVAVPGLVPDHTFFEHLANRRFPVTVWLREE 131 D ++ L + IP F+ + L K TGW+++AVPGL+P FF+HLANRRFPVT WLR Sbjct: 63 DGVEKLGLLDRIPDFEDVSTKLRKLTGWEIIAVPGLIPAAPFFDHLANRRFPVTNWLRTR 122 Query: 132 HEFDYIVEPDLFHDFFGHVPLLFNPVFANHLQEYGKGGLKALKLDGLAYLARLYWYTIEF 191 E DYIVEPD+FHDFFGHVP+L PVFA+ +Q YGK + L G + RLYWYT E+ Sbjct: 123 QELDYIVEPDMFHDFFGHVPVLSQPVFADFMQMYGKKAGDIIALGGDEMITRLYWYTAEY 182 Query: 192 GLIQ-SKEGLRIYGAGILSSGGEVEYCLSSDQPRRVPFEVERIMRTLYKIDTYQETYFVI 250 GL+Q + + L+ +GAG++SS E+++ + VPF++E +MRT Y+ID +Q YFV+ Sbjct: 183 GLVQEAGQPLKAFGAGLMSSFTELQFAVEGKDAHHVPFDLETVMRTGYEIDKFQRAYFVL 242 Query: 251 KDFEQLFND-TAPDFTPLYARLKQQEALPANTL 282 F+ L + DF + AR K Q+AL T+ Sbjct: 243 PSFDALRDAFQTADFEAIVARRKDQKALDPATV 275 >PA0872 [E] COG3186 Phenylalanine-4-hydroxylase Pae Pseudomonas aeruginosa Length = 262 Score = 200 bits (509), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 105/226 (46%), Positives = 137/226 (60%), Gaps = 3/226 (1%) Query: 43 YTPAQHALWRRLYERQAKLIPGRACDVFIDSLKALDV-SQGIPRFDRTTEALFKATGWQL 101 Y +H +W L RQ K+I GRAC ++D ++ L + + IP+ D L TGW++ Sbjct: 19 YPETEHQVWNTLITRQLKVIEGRACQEYLDGIEQLGLPHERIPQLDEINRVLQATTGWRV 78 Query: 102 VAVPGLVPDHTFFEHLANRRFPVTVWLREEHEFDYIVEPDLFHDFFGHVPLLFNPVFANH 161 VP L+P TFFE LA+++FPV ++R E DY+ EPD+FH+ FGH PLL NP FA Sbjct: 79 ARVPALIPFQTFFELLASQQFPVATFIRTPEELDYLQEPDIFHEIFGHCPLLTNPWFAEF 138 Query: 162 LQEYGKGGLKALKLDGLAYLARLYWYTIEFGLIQSKEGLRIYGAGILSSGGEVEYCLSSD 221 YGK GLKA K + +LARLYW TIEFGL+++ +G RIYG GILSS E Y L SD Sbjct: 139 THTYGKLGLKASK-EERVFLARLYWMTIEFGLVETDQGKRIYGGGILSSPKETVYSL-SD 196 Query: 222 QPRRVPFEVERIMRTLYKIDTYQETYFVIKDFEQLFNDTAPDFTPL 267 +P F MRT Y+ID Q YFV+ D ++LF D L Sbjct: 197 EPLHQAFNPLEAMRTPYRIDILQPLYFVLPDLKRLFQLAQEDIMAL 242 >VCA0828 [E] COG3186 Phenylalanine-4-hydroxylase Vch Vibrio cholerae Length = 289 Score = 191 bits (484), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 95/233 (40%), Positives = 139/233 (59%), Gaps = 2/233 (0%) Query: 47 QHALWRRLYERQAKLIPGRACDVFIDSLKALDV-SQGIPRFDRTTEALFKATGWQLVAVP 105 +HA+W L RQ +++ RAC ++D L L++ + +P+ L + TGWQ+ VP Sbjct: 46 EHAVWHELITRQQEVVKTRACQAYLDGLNMLNLPTDRLPQLPEINRVLQRETGWQVEPVP 105 Query: 106 GLVPDHTFFEHLANRRFPVTVWLREEHEFDYIVEPDLFHDFFGHVPLLFNPVFANHLQEY 165 L+ FF LA+++FPV +LR EFDY+ EPD FH+ +GH +L +P FA Y Sbjct: 106 ALISFDRFFALLADKKFPVATFLRRREEFDYLQEPDFFHEVYGHCAMLTHPDFAAFTHVY 165 Query: 166 GKGGLKALKLDGLAYLARLYWYTIEFGLIQSKEGLRIYGAGILSSGGEVEYCLSSDQPRR 225 G+ G KA + +YLARLYW+T+EFGL+Q + +IYG GILSS GE Y S P+R Sbjct: 166 GQLGAKATPKER-SYLARLYWFTVEFGLVQEQGQTKIYGGGILSSPGETLYASESTIPKR 224 Query: 226 VPFEVERIMRTLYKIDTYQETYFVIKDFEQLFNDTAPDFTPLYARLKQQEALP 278 PF++ +++RT Y+ID Q Y+V+ D QL+ + D L + Q LP Sbjct: 225 EPFDIMQVLRTPYRIDIMQPIYYVLPDLSQLYQLSQRDVMALVWQAMQDGLLP 277