BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0050.p (246 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0091 [E] COG0685 5,10-methylenetetrahydrofolate reductase Rso... 335 4e-92 PA0430 [E] COG0685 5,10-methylenetetrahydrofolate reductase Pae ... 293 3e-79 XF1121 [E] COG0685 5,10-methylenetetrahydrofolate reductase Xfa ... 232 6e-61 aq_1429 [E] COG0685 5,10-methylenetetrahydrofolate reductase Aae... 198 1e-50 STM4105 [E] COG0685 5,10-methylenetetrahydrofolate reductase Sty... 194 2e-49 >RSc0091 [E] COG0685 5,10-methylenetetrahydrofolate reductase Rso Ralstonia solanacearum Length = 276 Score = 335 bits (860), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 163/237 (68%), Positives = 192/237 (81%), Gaps = 6/237 (2%) Query: 1 MSTHNFSIEFFPPKTAEGAEKLRATRARLAALAPKYFSVTFGAGGSTQQGTRDTVLEIIG 60 M FS EFFPPKTAEGAEKLR TRA+L+ L P++ SVTFGAGG+TQQGT D V+EI Sbjct: 1 MQDRQFSFEFFPPKTAEGAEKLRNTRAQLSPLKPRFISVTFGAGGTTQQGTLDAVVEIQR 60 Query: 61 EGHEAAPHLSCIGSSRASLRAILEDYRAQGIKRLVALRGDLPSGYGAMDQASSEFRHANE 120 EG EAAPHLSC+GSSR S+RAIL+ YR GI+R+VALRGD+PSG G + EFR ANE Sbjct: 61 EGIEAAPHLSCVGSSRESIRAILDTYREHGIRRIVALRGDMPSGMGEI----GEFRFANE 116 Query: 121 LVEFVRAETGDWFHIEVAAYPEMHPQARSPQDDVQNFARKVKAGANSAITQYFYNADAYF 180 LVEF+R E D FHIEVAAYPE HPQARS + D++NFARKVKAGANSAITQYF+NADAYF Sbjct: 117 LVEFIRKEMNDTFHIEVAAYPEYHPQARSSRQDLENFARKVKAGANSAITQYFFNADAYF 176 Query: 181 RFVDEAARLGATVPVIAGIMPITNYTQLMRFSDMCGTEIPRWI--RLKLARDERXNL 235 +FVD+A +LG VP++ GIMPITN +QLMRFS+MCG EIPRWI RL+ D+R ++ Sbjct: 177 QFVDDARKLGVDVPIVPGIMPITNSSQLMRFSEMCGAEIPRWIAKRLESFGDDRESI 233 >PA0430 [E] COG0685 5,10-methylenetetrahydrofolate reductase Pae Pseudomonas aeruginosa Length = 290 Score = 293 bits (750), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 145/227 (63%), Positives = 171/227 (75%), Gaps = 4/227 (1%) Query: 2 STHNFSIEFFPPKTAEGAEKLRATRARLAALAPKYFSVTFGAGGSTQQGTRDTVLEIIGE 61 S FS EFFP KT G EKL AT LA P +FS T+GAGGST+ T TVL++ GE Sbjct: 12 SERRFSFEFFPAKTEAGHEKLLATARNLAGYKPDFFSCTYGAGGSTRDRTLSTVLQLDGE 71 Query: 62 GH-EAAPHLSCIGSSRASLRAILEDYRAQGIKRLVALRGDLPSGYGAMDQASSEFRHANE 120 APHLSC+G S+A LR +L YR GI+R+VALRGDLPSG G AS E R+ANE Sbjct: 72 VKVPTAPHLSCVGDSKAELRELLGRYREAGIRRIVALRGDLPSGMG---MASGELRYANE 128 Query: 121 LVEFVRAETGDWFHIEVAAYPEMHPQARSPQDDVQNFARKVKAGANSAITQYFYNADAYF 180 LV+F+R ETGD FHIEVAAYPE+HPQARS +DD+ NF RKVKAGA+SAITQYF+NADAYF Sbjct: 129 LVDFIRTETGDHFHIEVAAYPEVHPQARSFEDDLANFVRKVKAGASSAITQYFFNADAYF 188 Query: 181 RFVDEAARLGATVPVIAGIMPITNYTQLMRFSDMCGTEIPRWIRLKL 227 FV+ A+LG +PV+ GIMPITNY++L RFSD CG E+PRWIR +L Sbjct: 189 YFVERVAKLGVDIPVVPGIMPITNYSKLARFSDACGAELPRWIRKQL 235 >XF1121 [E] COG0685 5,10-methylenetetrahydrofolate reductase Xfa Xylella fastidiosa 9a5c Length = 275 Score = 232 bits (592), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 117/222 (52%), Positives = 151/222 (68%), Gaps = 5/222 (2%) Query: 7 SIEFFPPKTAEGAEKLRATRARLAALAPKYFSVTFGAGGSTQQGTRDTVLEII-GEGHEA 65 S EF+PPK + +L T RL P+Y S TFGAGGST T +TV + G +A Sbjct: 5 SFEFYPPKNDDQRAQLDRTANRLRTFTPEYVSCTFGAGGSTLSYTSETVRHLSQHHGFDA 64 Query: 66 APHLSCIGSSRASLRAILEDYRAQGIKRLVALRGDLPSGYGAMDQASSEFRHANELVEFV 125 APHLSC+G SR +R +L+ YRA G +R+VALRGDLPSG G + R+A +L+ F+ Sbjct: 65 APHLSCVGGSRQEIRELLKLYRAIGCQRIVALRGDLPSGMGH----PGDLRYAADLITFI 120 Query: 126 RAETGDWFHIEVAAYPEMHPQARSPQDDVQNFARKVKAGANSAITQYFYNADAYFRFVDE 185 RAE GD FH+E+ AYPE HPQA + +D+ F K AGA++AITQYFYN DAYF FVD Sbjct: 121 RAEHGDHFHLEIGAYPETHPQASNTLNDLHYFKAKADAGADAAITQYFYNPDAYFHFVDA 180 Query: 186 AARLGATVPVIAGIMPITNYTQLMRFSDMCGTEIPRWIRLKL 227 RLG T+P++AG+MPI+N+ +L FS+ CG EIPRWI K+ Sbjct: 181 VQRLGVTIPIVAGVMPISNFDKLHHFSEQCGAEIPRWITKKM 222 >aq_1429 [E] COG0685 5,10-methylenetetrahydrofolate reductase Aae Aquifex aeolicus Length = 296 Score = 198 bits (503), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 100/228 (43%), Positives = 143/228 (62%), Gaps = 1/228 (0%) Query: 5 NFSIEFFPPKTAEGAEKLRATRARLAALAPKYFSVTFGAGGSTQQGTRDTVLEIIGEGH- 63 + S EFFPPKT EG +L T +L L P + SVT+GAGGST+ TR+ V +I E + Sbjct: 13 SISFEFFPPKTEEGERQLFETIRKLEKLNPTFVSVTYGAGGSTRDRTRNIVQKIHEETNL 72 Query: 64 EAAPHLSCIGSSRASLRAILEDYRAQGIKRLVALRGDLPSGYGAMDQASSEFRHANELVE 123 HL+CI +R L IL+DY+ GI+ ++ALRGD+P ++A ELVE Sbjct: 73 TVMAHLTCIAHTREELIDILQDYKNIGIENILALRGDVPRDKPDWRPPKGACKYAKELVE 132 Query: 124 FVRAETGDWFHIEVAAYPEMHPQARSPQDDVQNFARKVKAGANSAITQYFYNADAYFRFV 183 +R E GDWF I VA+YPE HP++ + + +V+ F KV+AGA+ +ITQ F+ D Y+RFV Sbjct: 133 LIRKEFGDWFSIGVASYPEGHPESPNLEWEVKYFKEKVEAGADFSITQMFFVNDYYYRFV 192 Query: 184 DEAARLGATVPVIAGIMPITNYTQLMRFSDMCGTEIPRWIRLKLARDE 231 + G + +I GIMPITN+ Q+ +F+ +CG IP+ + KL + E Sbjct: 193 EMCKNAGIDISIIPGIMPITNFKQIRKFASLCGATIPQSLIEKLEKVE 240 >STM4105 [E] COG0685 5,10-methylenetetrahydrofolate reductase Sty Salmonella typhimurium LT2 Length = 296 Score = 194 bits (493), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 102/223 (45%), Positives = 141/223 (63%), Gaps = 11/223 (4%) Query: 5 NFSIEFFPPKTAEGAEKLRATRARLAALAPKYFSVTFGAGGSTQQGTRDTVLEIIGE--G 62 N S EFFPP+T+E + L + RL++L PK+ SVT+GA + T +V++ I E G Sbjct: 24 NVSFEFFPPRTSEMEQTLWNSIDRLSSLKPKFVSVTYGANSGERDRTH-SVIKGIKERTG 82 Query: 63 HEAAPHLSCIGSSRASLRAILEDYRAQGIKRLVALRGDLPSGYGAMDQASSEFRHANELV 122 EAAPHL+CI ++R LR I DY GI+ +VALRGDLP G G + +A +LV Sbjct: 83 LEAAPHLTCIDATRDELRTIARDYWNNGIRHIVALRGDLPPGSGKPEM------YAADLV 136 Query: 123 EFVRAETGDWFHIEVAAYPEMHPQARSPQDDVQNFARKVKAGANSAITQYFYNADAYFRF 182 ++ E D F I VAAYPE+HP+A+S Q D+ N RKV AGAN AITQ+F++ ++Y RF Sbjct: 137 GLLK-EVAD-FDISVAAYPEVHPEAKSAQADLLNLKRKVDAGANRAITQFFFDVESYLRF 194 Query: 183 VDEAARLGATVPVIAGIMPITNYTQLMRFSDMCGTEIPRWIRL 225 D G V +I GI+P++N+ Q +F+DM IP W+ L Sbjct: 195 RDRCVSAGIDVEIIPGILPVSNFKQAKKFADMTNVRIPSWMSL 237