BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0027.0006.p (392 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BMEII0291 [R] COG0579 Predicted dehydrogenase Bme Brucella melit... 329 6e-90 SMb20101 [R] COG0579 Predicted dehydrogenase Sme Sinorhizobium m... 308 2e-83 alr2826 [R] COG0579 Predicted dehydrogenase Nos Nostoc sp. PCC 7120 293 4e-79 ygaF [R] COG0579 Predicted dehydrogenase Eco Escherichia coli K12 273 5e-73 ZygaF [R] COG0579 Predicted dehydrogenase EcZ Escherichia coli O... 271 3e-72 >BMEII0291 [R] COG0579 Predicted dehydrogenase Bme Brucella melitensis Length = 410 Score = 329 bits (844), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 167/394 (42%), Positives = 240/394 (60%), Gaps = 14/394 (3%) Query: 3 YAVIGGGINGLAVARQLLLDQPDVRVTVFEKEDAVARHQSSHNSGV----------TLKA 52 Y VIGGGI GLA A+ + +P R+ V EKE +ARHQ+ HNSGV +LKA Sbjct: 6 YCVIGGGIVGLATAKAVQEAEPGARIIVLEKESGLARHQTGHNSGVIHAGIYYQPGSLKA 65 Query: 53 RLCRRGVELVRTYCEQNGLPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDGA 112 RLCR G + + +C+Q +P++ CGK++VA A E+ R+EA+ R+A+ N + + +D Sbjct: 66 RLCRAGAQATKAFCKQYSIPFESCGKLLVATSALEMERMEALARRAVQNNI-EFSHLDQQ 124 Query: 113 RLREIEPHCVGLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQHVQ 172 LR+ EP GL AL+ P T IV Y ++++ MA EIV+RGG++RLN+ V + E + V+ Sbjct: 125 ALRKAEPAISGLGALFVPATGIVDYAKVSRAMAAEIVERGGIVRLNSPVTAIHEDEKGVE 184 Query: 173 IELAGGEVHGDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHVRGL 232 + G V +AC GLQSDR+A+ +G T RIVPF G+YY + + VR L Sbjct: 185 VVSGGETVRASKL---VACAGLQSDRIARLAGLDITHRIVPFRGEYYTLPQTRAGIVRHL 241 Query: 233 IYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPGFWK 292 IYP+PDP PFLG+H T+ IDG +T+GPNA + RE Y F D+AD + GFWK Sbjct: 242 IYPIPDPDLPFLGIHLTRTIDGGVTVGPNAVLGFSREGYAKGSFHPGDIADMSFFAGFWK 301 Query: 293 FASRNMAATLRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLVDDF 352 A +N + + E S ++++ +Y P+L + GIRAQA+ DG LV DF Sbjct: 302 MAMKNWRSAISEFGNSTSRARYLKQCRKYCPSLELSDLGAPGAGIRAQAVLDDGTLVHDF 361 Query: 353 VIRQSSRVTHIRNAPSPGATSSLAIGEHIVRNLI 386 + +++ R+ H+ NAPSP ATSS+ IG I + L+ Sbjct: 362 LFKETERMLHVCNAPSPAATSSIPIGRMIAQKLL 395 >SMb20101 [R] COG0579 Predicted dehydrogenase Sme Sinorhizobium meliloti Length = 426 Score = 308 bits (788), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 162/394 (41%), Positives = 226/394 (57%), Gaps = 14/394 (3%) Query: 3 YAVIGGGINGLAVARQLLLDQPDVRVTVFEKEDAVARHQSSHNSGV----------TLKA 52 Y +IGGGI GLA A L + + EKE +ARHQ+ HNSGV +LKA Sbjct: 15 YCIIGGGIVGLATAMALQERMGGASIVLLEKERELARHQTGHNSGVIHAGIYYAPGSLKA 74 Query: 53 RLCRRGVELVRTYCEQNGLPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDGA 112 +LCR G E + +C NG+ ++ CGK++VA + E+ R+E++ +A NG+ + + + Sbjct: 75 KLCREGAEATKEFCTGNGISFETCGKLLVATNDAEIERMESLEERAQQNGI-EYTRLSKS 133 Query: 113 RLREIEPHCVGLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQHVQ 172 +LR EP+ GL AL T IV Y + + MA+ I RGG IR + E+ V+ Sbjct: 134 QLRSDEPNIAGLSALLVHATGIVDYSAVCRAMAERIEVRGGEIRCGVEATAIAEEDGGVR 193 Query: 173 IELAGGEVHGDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHVRGL 232 I A G + IAC GLQSDR+A +G RIVPF G+YYV+ + R L Sbjct: 194 IASATGRIEARRL---IACAGLQSDRIALMAGLSIDHRIVPFRGEYYVLPASKAGVTRRL 250 Query: 233 IYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPGFWK 292 IYP+PDP PFLG+H T+ IDG MT+GPNA + RE Y F DVA+ +PGFWK Sbjct: 251 IYPIPDPNLPFLGIHLTRTIDGGMTVGPNAVLGFAREGYPKGSFKAADVANMAAFPGFWK 310 Query: 293 FASRNMAATLRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLVDDF 352 A++N + + E S ++RE +Y P+L+ + GIRAQA+ DG LV DF Sbjct: 311 MAAKNWRSAITEFANSASRFRYLRECRKYCPSLTIDDLAVPQAGIRAQAVMADGSLVHDF 370 Query: 353 VIRQSSRVTHIRNAPSPGATSSLAIGEHIVRNLI 386 + +Q+ R+ H+ NAPSP ATS++ IG IV L+ Sbjct: 371 LFKQTERMLHVCNAPSPAATSAIPIGRMIVDRLL 404 >alr2826 [R] COG0579 Predicted dehydrogenase Nos Nostoc sp. PCC 7120 Length = 404 Score = 293 bits (750), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 159/392 (40%), Positives = 224/392 (57%), Gaps = 20/392 (5%) Query: 3 YAVIGGGINGLAVARQLLLDQPDVRVTVFEKEDAVARHQSSHNSGV----------TLKA 52 + ++GGGI GL+ L P R+ V EKE A HQ+ +NSGV + KA Sbjct: 4 FTIVGGGIVGLSTGMALGKRYPQARILVLEKESQWAFHQTGNNSGVIHSGIYYKPGSFKA 63 Query: 53 RLCRRGVELVRTYCEQNGLPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDGA 112 + CR G + + +C+ G+ ++ CGKV+VA + ELPRLE ++++ L NG V+ I Sbjct: 64 KFCRDGRDSMVKFCQDYGIDHEVCGKVIVATNEQELPRLENLYQRGLENGT-QVQKISAE 122 Query: 113 RLREIEPH--CV-GLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQ 169 ++EIEP+ CV G+R T IV+Y Q+ + I Q+GG +RLN V ++ G Sbjct: 123 EVKEIEPYVKCVAGIRVF---STGIVNYKQVCLKYVELIQQQGGDLRLNTKVLKISPSGN 179 Query: 170 HVQIELAGGEVHGDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHV 229 + +E G F I C GL SDR+A+ G + +IVPF G+YY + + V Sbjct: 180 NHVLETNNGNFETR---FVINCAGLHSDRIAKLGGVKPSAKIVPFRGEYYELTPEKRYLV 236 Query: 230 RGLIYPVPDPRFPFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPG 289 + LIYPVP+P FPFLGVHFT+ IDG + GPNA +SL RE Y+ F LRD A+ + YPG Sbjct: 237 KTLIYPVPNPEFPFLGVHFTRMIDGSVHAGPNAVLSLKREGYKKTDFDLRDFAEVMTYPG 296 Query: 290 FWKFASRNMAATLRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLV 349 FWK A ++ ++E+ S+ F R +P + + P G+RAQA+ DG+LV Sbjct: 297 FWKLAGKHADEGIQEIIRSFSKAAFTRSLQNLIPEVQAEDLVPTHAGVRAQALMDDGKLV 356 Query: 350 DDFVIRQSSRVTHIRNAPSPGATSSLAIGEHI 381 DDF I H+ NAPSP ATSSL IG+ I Sbjct: 357 DDFYIVPGENSIHVCNAPSPAATSSLEIGKAI 388 >ygaF [R] COG0579 Predicted dehydrogenase Eco Escherichia coli K12 Length = 444 Score = 273 bits (698), Expect = 5e-73, Method: Compositional matrix adjust. Identities = 149/381 (39%), Positives = 211/381 (55%), Gaps = 15/381 (3%) Query: 13 LAVARQLLLDQPDVRVTVFEKEDAVARHQSSHNSGV----------TLKARLCRRGVELV 62 ++ A QL+ PD R+ + EKE A A HQ+ HNSGV +LKA+ C G Sbjct: 36 MSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRAT 95 Query: 63 RTYCEQNGLPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDGARLREIEPHCV 122 + +C+QNG+ YD CGK++VA E+ R+ A+ + ANG+ + E ++ LRE EP+ Sbjct: 96 KAFCDQNGIRYDNCGKMLVATSDLEMERMRALWERTAANGI-EREWLNADELREREPNIT 154 Query: 123 GLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQHVQIEL-AGGEVH 181 GL ++ P + IVSY + MA+ RGG I NA V + E V I GGE Sbjct: 155 GLGGIFVPSSGIVSYRDVTAAMAKIFQSRGGEIIYNAEVSGLNEHKNGVVIRTRQGGEYE 214 Query: 182 GDAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHVRGLIYPVPDPRF 241 I+C GL +DRL + G I PF G+Y+ + V LIYP+PDP Sbjct: 215 ASTL---ISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHLIYPIPDPAM 271 Query: 242 PFLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPGFWKFASRNMAAT 301 PFLGVH T+ IDG +T+GPNA ++ RE Y+ FS D + LG G + ++ + Sbjct: 272 PFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSG 331 Query: 302 LRELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLVDDFVIRQSSRVT 361 L E+K + + ++R +Y P LS + P G+RAQA+ PDG+L+DDF+ + R Sbjct: 332 LGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTI 391 Query: 362 HIRNAPSPGATSSLAIGEHIV 382 H NAPSP ATS++ IG HIV Sbjct: 392 HTCNAPSPAATSAIPIGAHIV 412 >ZygaF [R] COG0579 Predicted dehydrogenase EcZ Escherichia coli O157:H7 EDL933 Length = 444 Score = 271 bits (692), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 146/383 (38%), Positives = 212/383 (55%), Gaps = 13/383 (3%) Query: 13 LAVARQLLLDQPDVRVTVFEKEDAVARHQSSHNSGV----------TLKARLCRRGVELV 62 ++ A QL+ PD R+ + EKE A HQ+ HNSGV +LKA+ C G Sbjct: 36 MSTAMQLIDVYPDARIALLEKESGPACHQTGHNSGVIHAGVYYTPGSLKAQFCLAGNRAT 95 Query: 63 RTYCEQNGLPYDECGKVVVALDADELPRLEAIHRKALANGVPDVEMIDGARLREIEPHCV 122 + +C+QNG+ YD CGK++VA E+ R+ A+ + ANG+ + E ++ LRE EP+ Sbjct: 96 KAFCDQNGIRYDNCGKMLVATSELEMERMRALWERTAANGI-EREWLNADELREREPNIT 154 Query: 123 GLRALYSPRTAIVSYGQIAQHMAQEIVQRGGVIRLNAAVQRVCEQGQHVQIELAGGEVHG 182 GL ++ P + IVSY ++ MA+ RGG I NA V + E V I G + Sbjct: 155 GLGGIFVPSSGIVSYREVTAAMAKIFQARGGEIIYNAEVSGLSEHKSGVVIRTRQGSDYE 214 Query: 183 DAFDFAIACCGLQSDRLAQQSGDVATPRIVPFFGQYYVIDEAFKSHVRGLIYPVPDPRFP 242 + I+C GL +DRL + G I PF G+Y+ + V LIYP+PDP P Sbjct: 215 AST--LISCSGLMADRLVKMXGLEPGFIICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMP 272 Query: 243 FLGVHFTKRIDGQMTIGPNAFISLGRENYQGDRFSLRDVADYLGYPGFWKFASRNMAATL 302 FLGVH T+ IDG +T+GPNA ++ RE Y+ FS D + LG G + ++ + L Sbjct: 273 FLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGL 332 Query: 303 RELKTVVSERHFVREAARYVPALSEVSVTPAVRGIRAQAMEPDGRLVDDFVIRQSSRVTH 362 E+K + + ++R +Y P LS + P G+RAQA+ PDG+L+DDF+ + R H Sbjct: 333 GEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIH 392 Query: 363 IRNAPSPGATSSLAIGEHIVRNL 385 NAPSP ATS++ IG HIV + Sbjct: 393 TCNAPSPAATSAIPIGAHIVSKV 415