BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0024.0167.p (536 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mhpA [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and r... 417 e-116 ZmhpA [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and ... 415 e-115 ECs0402 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase an... 415 e-115 Cgl1132 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase an... 169 2e-41 STM1546 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase an... 113 1e-24 >mhpA [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Eco Escherichia coli K12 Length = 554 Score = 417 bits (1072), Expect = e-116, Method: Compositional matrix adjust. Identities = 222/517 (42%), Positives = 310/517 (59%), Gaps = 16/517 (3%) Query: 8 QVLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQSAGMADAI 67 QV I GAGPVG+ +AN LG GI L++E+ ++DYPRA+GIDDEALRT QS G+ D + Sbjct: 17 QVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVDDV 76 Query: 68 VRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLGEATLRQGLQRFPHVELR 127 + MR T RCFA+IQP T EFGW RRN F QP +A + +G+ RFP+V Sbjct: 77 LPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCL 136 Query: 128 SGVELLDLVQDEQGVLATISDAHGMQSYLAAQYAVGCDGGRSTVREKILKLPYEGKTHPA 187 EL Q + V + A G + + AQ+ V CDGG S VR + L +P+EGKT P Sbjct: 137 FSRELEAFSQQDDEVTLHLKTAEGQREIVKAQWLVACDGGASFVR-RTLNVPFEGKTAPN 195 Query: 188 KWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFPGEDGEQMLAPVKVRE 247 +W+V++ DPL P+ L C+P RPYV ALP+ VRR+EFM+ PGE EQ+ P +R+ Sbjct: 196 QWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRK 255 Query: 248 LLREHVADVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWIGQGLNAGLRDA 307 LL + + + D + +IR RVYTHN+R+A R+ LAGDAAHI P W GQG N+G+RDA Sbjct: 256 LLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDA 315 Query: 308 FNLAWKLAWVLQGRMKPALLASYHDERHAHVKAMIDLADLFGAVLSQKNRLLAWCRDKFF 367 FNLAWKLA V+QG+ + ALL +Y ER H KAMIDL+ G VL+ R RD Sbjct: 316 FNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVS 375 Query: 368 LAIKDIPRVRDYVLQMKFKPMPSFHSQAVMQGDPRNRDDLVGRMFLQSLVENGEGEVMRL 427 + +P V+ Y L+M+FKPMP ++ A+M+ + + VG+MF+Q V G+V L Sbjct: 376 WLLNYLPPVKRYFLEMRFKPMPQYYGGALMR-EGEAKHSPVGKMFIQPKVTLENGDVTLL 434 Query: 428 DDLCGSRFTLLSWRCDVLPAATVDPDLRRRMQAFGCDYLQGV------RSRSGARGVPLT 481 D+ G+ F ++ W C+ P + + ++ +A G ++Q V ++ GV Sbjct: 435 DNAIGANFAVIGWGCN--PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRV 492 Query: 482 GSGAVIQDHENKLNAWFKEKGVDWVLLRPDRFIAAAG 518 G D + +L +WF + V++RPDRF+AA Sbjct: 493 G------DTQGRLRSWFAQHNASLVVMRPDRFVAATA 523 >ZmhpA [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases EcZ Escherichia coli O157:H7 EDL933 Length = 554 Score = 415 bits (1067), Expect = e-115, Method: Compositional matrix adjust. Identities = 222/518 (42%), Positives = 311/518 (60%), Gaps = 18/518 (3%) Query: 8 QVLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQSAGMADAI 67 QV I GAGPVG+ +AN LG GI L++E+ ++DYPRA+GIDDEALRT QS G+ D + Sbjct: 17 QVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVDNV 76 Query: 68 VRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLGEATLRQGLQRFPHVELR 127 + MR T RCFA+IQP T EFGW RRN F QP +A + +G+ RFP+V Sbjct: 77 LPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCL 136 Query: 128 SGVELLDLVQDEQGVLATISDAHGMQSYLAAQYAVGCDGGRSTVREKILKLPYEGKTHPA 187 EL Q + V + A G++ + AQ+ V CDGG S VR + L +P+EGKT P Sbjct: 137 FSRELEAFSQQDDEVTLHLKTAEGLREIVKAQWLVACDGGASFVR-RTLNVPFEGKTAPN 195 Query: 188 KWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFPGEDGEQMLAPVKVRE 247 +W+V++ DPL P+ L C+P RPYV ALP+ VRR+EFM+ PGE EQ+ P +R+ Sbjct: 196 QWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRK 255 Query: 248 LLREHVADVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWIGQGLNAGLRDA 307 LL + + + D + +IR RVYTHN+R+A R+ LAGDAAHI P W GQG N+G+RDA Sbjct: 256 LLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDA 315 Query: 308 FNLAWKLAWVLQGRMKPALLASYHDERHAHVKAMIDLADLFGAVLSQKNRLLAWCRDKFF 367 FNLAWKLA V+QG+ + ALL +Y ER H KAMIDL+ G VL+ R RD Sbjct: 316 FNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVS 375 Query: 368 LAIKDIPRVRDYVLQMKFKPMPSFHSQA-VMQGDPRNRDDLVGRMFLQSLVENGEGEVMR 426 + +P V+ Y L+M+FKPMP ++ A V +G+ ++ VG+MF+Q V G+V Sbjct: 376 WLLNYLPPVKRYFLEMRFKPMPQYYGGALVREGEAKHSP--VGKMFIQPKVTLENGDVTL 433 Query: 427 LDDLCGSRFTLLSWRCDVLPAATVDPDLRRRMQAFGCDYLQGV------RSRSGARGVPL 480 LD+ G+ F ++ W C+ P + + ++ +A ++Q V ++ GV Sbjct: 434 LDNAIGANFAVIGWGCN--PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLR 491 Query: 481 TGSGAVIQDHENKLNAWFKEKGVDWVLLRPDRFIAAAG 518 G D + +L +WF + V++RPDRF+AA Sbjct: 492 VG------DTQGRLRSWFAQHNASLVVMRPDRFVAATA 523 >ECs0402 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Ecs Escherichia coli O157:H7 Length = 554 Score = 415 bits (1067), Expect = e-115, Method: Compositional matrix adjust. Identities = 222/518 (42%), Positives = 311/518 (60%), Gaps = 18/518 (3%) Query: 8 QVLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQSAGMADAI 67 QV I GAGPVG+ +AN LG GI L++E+ ++DYPRA+GIDDEALRT QS G+ D + Sbjct: 17 QVAIAGAGPVGLMMANYLGQMGIDVLVVEKLDKLIDYPRAIGIDDEALRTMQSVGLVDNV 76 Query: 68 VRDVIQNVPMRMYTADRRCFAEIQPTTREFGWYRRNLFSQPLGEATLRQGLQRFPHVELR 127 + MR T RCFA+IQP T EFGW RRN F QP +A + +G+ RFP+V Sbjct: 77 LPHTTPWHAMRFLTPKGRCFADIQPMTDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCL 136 Query: 128 SGVELLDLVQDEQGVLATISDAHGMQSYLAAQYAVGCDGGRSTVREKILKLPYEGKTHPA 187 EL Q + V + A G++ + AQ+ V CDGG S VR + L +P+EGKT P Sbjct: 137 FSRELEAFSQQDDEVTLHLKTAEGLREIVKAQWLVACDGGASFVR-RTLNVPFEGKTAPN 195 Query: 188 KWVVIECDGDPLDAPYTALHCEPQRPYVCLALPYGVRRWEFMLFPGEDGEQMLAPVKVRE 247 +W+V++ DPL P+ L C+P RPYV ALP+ VRR+EFM+ PGE EQ+ P +R+ Sbjct: 196 QWIVVDIANDPLSTPHIYLCCDPVRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRK 255 Query: 248 LLREHVADVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHITPPWIGQGLNAGLRDA 307 LL + + + D + +IR RVYTHN+R+A R+ LAGDAAHI P W GQG N+G+RDA Sbjct: 256 LLSKVLPNPDNVELIRQRVYTHNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDA 315 Query: 308 FNLAWKLAWVLQGRMKPALLASYHDERHAHVKAMIDLADLFGAVLSQKNRLLAWCRDKFF 367 FNLAWKLA V+QG+ + ALL +Y ER H KAMIDL+ G VL+ R RD Sbjct: 316 FNLAWKLALVIQGKARDALLDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVS 375 Query: 368 LAIKDIPRVRDYVLQMKFKPMPSFHSQA-VMQGDPRNRDDLVGRMFLQSLVENGEGEVMR 426 + +P V+ Y L+M+FKPMP ++ A V +G+ ++ VG+MF+Q V G+V Sbjct: 376 WLLNYLPPVKRYFLEMRFKPMPQYYGGALVREGEAKHSP--VGKMFIQPKVTLENGDVTL 433 Query: 427 LDDLCGSRFTLLSWRCDVLPAATVDPDLRRRMQAFGCDYLQGV------RSRSGARGVPL 480 LD+ G+ F ++ W C+ P + + ++ +A ++Q V ++ GV Sbjct: 434 LDNAIGANFAVIGWGCN--PLWGMSDEQIQQWRALSTRFIQVVPEVQIHTAQDNHDGVLR 491 Query: 481 TGSGAVIQDHENKLNAWFKEKGVDWVLLRPDRFIAAAG 518 G D + +L +WF + V++RPDRF+AA Sbjct: 492 VG------DTQGRLRSWFAQHNASLVVMRPDRFVAATA 523 >Cgl1132 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Cgl Corynebacterium glutamicum Length = 530 Score = 169 bits (427), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 151/523 (28%), Positives = 222/523 (42%), Gaps = 44/523 (8%) Query: 7 AQVLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQSAGMADA 66 V I+G GP G LA LLG G I+E+ + PRAV D E R G+ D+ Sbjct: 8 TDVCIVGGGPTGTLLAVLLGQKGHRVTILEKWPTFYERPRAVTFDHEIARILGYIGI-DS 66 Query: 67 IVRDVI--QNVPMRMYTADRRCFAEIQPTT-REFGWYRRNLFSQPLGEATLRQGLQRFPH 123 + I + A E+ T+ + GW R F QP E LR Sbjct: 67 ENDEAIDYHSDSYDWKNAAGETLLEVDWTSMTDSGWRTRYWFYQPELEKRLRDLALTMDF 126 Query: 124 VELRSGVELLDLVQDEQGVL--ATISD------AHGMQSYLAAQYAVGCDGGRSTVREKI 175 V++R G + L QDE + ++D A + + A+Y +G DG S VR Sbjct: 127 VDIRCGFTAVGLSQDENSAIIHGIVTDTPENIPADAQREDIRAKYVIGADGANSFVRNS- 185 Query: 176 LKLPYEGKTHPAKWVVIECDGDPLDAPYTALH---CEPQRPYVCLALPYGVRRWEFMLFP 232 L L + W++++ D Y H C+P+RP + G RRWEFM P Sbjct: 186 LGLEMNDLGYFFDWLILDLK-PTQDIDYGTDHWQLCDPKRPTTIVPGGPGRRRWEFMALP 244 Query: 233 GEDGEQMLAPVKVRELLREHVADVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHIT 292 GED +++ + LL + R+ VY +R A GR +AGDAAH+ Sbjct: 245 GEDLKELASEESAWNLLEPWDVTPGKAILERSAVYRFQARWAQEWRSGRALIAGDAAHLM 304 Query: 293 PPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPALLASYHDERHAHVKAMIDLADLFGAVL 352 PP+ G+G+ AGLRD+ LAW+L VL G+ ALL +Y +ER HV ID + G V+ Sbjct: 305 PPFAGEGMCAGLRDSLALAWRLDLVLSGKSDDALLDTYGEERREHVHYYIDFSMDLGNVI 364 Query: 353 SQKNRLLAWCRDKFFLAIKDIPRVRDYVLQMKFKPM-PSFHSQAVMQGDPRNRDDLVGRM 411 + A RD+ IK++ + + P + G G + Sbjct: 365 CITDEDEARLRDERM--IKELEAQDGVPVNTDVAHLGPGIWDKDSSHG---------GEL 413 Query: 412 FLQSLVENGEGEVMRLDDLCGSRFTLLSWRCDVLPAATVDPDLRRRMQAFGCDYLQGVRS 471 Q +VE +G R DD G + +L D P +D D + A G ++ V Sbjct: 414 AKQGIVEY-QGRKARFDDAVGRGWAVLGLNTD--PREVLDEDSLVALDAIGA-IVESVGD 469 Query: 472 RSGARGVPLTGSGAVIQDHENKLNAWFKEKGVDWVLLRPDRFI 514 + A + D E W KE G +++ RPD ++ Sbjct: 470 ATSA-----------VLDVEGLYTRWLKEAGATFIITRPDFYV 501 >STM1546 [HC] COG0654 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases Sty Salmonella typhimurium LT2 Length = 502 Score = 113 bits (282), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 105/372 (28%), Positives = 166/372 (44%), Gaps = 15/372 (4%) Query: 1 MSTAVQAQVLIIGAGPVGVTLANLLGTYGISTLIIERNLDVLDYPRAVGIDDEALRTFQS 60 M+ VLI GAG G+TLA L +G+S +IE+ R GI F+ Sbjct: 1 MTRYTTTDVLICGAGVTGLTLAIELARHGVSFRLIEKRTTPFIGSRGKGIQPRTQEIFED 60 Query: 61 AGMADAIVRDVIQNVPMRMY-----TADRRCFAEIQPTTREFGWYRRNLFSQPLGEATLR 115 G+ + +V +R Y D +PT E ++ + Q + E +R Sbjct: 61 LGILNKVVAAGGLYPRLRTYRHDGSYVDSDIAHHTKPTHAE-PYHLPLMVPQNVTETIMR 119 Query: 116 QGLQRFPHVELRSGVELLDLVQDEQGVLATISDAHGMQSYLAAQYAVGCDGGRSTVREKI 175 + L+ + H + G EL Q + V A ++ G + + A Y +G DGG S VR+K Sbjct: 120 EQLKAWGH-RVEFGCELRHFAQTPRTVTAYVAGPAG-EEVIIAHYLIGADGGGSFVRKK- 176 Query: 176 LKLPYEGKTHPAKWVVIECDGDPLDAPYTALHCEPQRPYVCLALPY-GVRRWEFMLFPGE 234 L + + G+T +V + L+ + + P G + ++ Sbjct: 177 LGVSFPGRTLGIHALVADASLSGLNRDVWHHFNDGDMARMITICPLAGTQLFQIQALLAP 236 Query: 235 DGEQMLAPVKVRELLREHVA--DVDALNIIRARVYTHNSRVAGSLVCGRICLAGDAAHIT 292 D Q + + L E + DV +I Y N+R+A G++ LAGDAAH+ Sbjct: 237 DDSQNFSADVLTAFLTERIGRTDVRIHSIPWVSKYQMNARIAEHYRVGKVFLAGDAAHVH 296 Query: 293 PPWIGQGLNAGLRDAFNLAWKLAWVLQGRMKPALLASYHDERHAHVKAMIDLADLFGAVL 352 PP GQGLN ++DA+NL WK+A L+G + LL SY ER ++++ L+ + Sbjct: 297 PPTGGQGLNTSIQDAYNLGWKMAASLRGAGE-ELLDSYEQERRPIAESLLHLSTRL--LD 353 Query: 353 SQKNRLLAWCRD 364 SQK R + RD Sbjct: 354 SQKQRGIKRERD 365