BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0209.p (267 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC1427 [E] COG1176 ABC-type spermidine/putrescine transport syst... 147 2e-35 PA0325 [E] COG1176 ABC-type spermidine/putrescine transport syst... 145 8e-35 PM0263 [E] COG1176 ABC-type spermidine/putrescine transport syst... 144 2e-34 CAC0839 [E] COG1176 ABC-type spermidine/putrescine transport sys... 139 5e-33 mlr4191 [E] COG1176 ABC-type spermidine/putrescine transport sys... 132 8e-31 >VC1427 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Vch Vibrio cholerae Length = 286 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 78/211 (36%), Positives = 134/211 (63%), Gaps = 4/211 (1%) Query: 45 LVPLVLTFVLSFNAYDAETGPKAGTFTLEHYTAVLTDPYYYGIFWRTLWISGLVTLICIV 104 L+P ++ SF D E FT E+YT +L DP Y + + +++ + TLIC+V Sbjct: 25 LIPNLMIIGTSFLTRD-EANLIKFAFTFENYTRLL-DPLYGKVMLHSFYMAIVATLICLV 82 Query: 105 VGAPEAWILNRMRKPWRSIMLLVVLAPLMISVVVRAFGWSMLLGPEGLINGTLVALGF-- 162 +G P A+I+ +M + WR ML +++ P + ++R +G ++LG +G++N L++LG Sbjct: 83 IGYPFAYIVAKMPEKWRPFMLFLIIVPFWTNSLIRTYGLKIVLGTQGILNQALMSLGLID 142 Query: 163 AKVKLLYTETAIVIALVHVMLPFMVIPVWTSLQKLDGGVANAALSLGASPITVLRRIVLP 222 A +++++TETA++I LV+++LPFM++P++++++KLDG AA LGA+ L R++LP Sbjct: 143 APLRIMFTETAVMIGLVYILLPFMILPLYSAIEKLDGTYIEAARDLGANKFQTLVRVILP 202 Query: 223 QIMPGILSGSLIVFGLSASSFAIPGLLGGRR 253 MPGI+ G L+V + F I LLGG + Sbjct: 203 LTMPGIIGGCLLVLLPALGMFYIADLLGGAK 233 >PA0325 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Pae Pseudomonas aeruginosa Length = 309 Score = 145 bits (367), Expect = 8e-35, Method: Compositional matrix adjust. Identities = 85/242 (35%), Positives = 140/242 (57%), Gaps = 12/242 (4%) Query: 33 SAPAILLCTVMLLVPLVLTFVLSFNAYDAETGPKAGTFTLEHY----------TAVLTDP 82 S+PA+L+ + L++PL + F +S G K G +LE Y +++ + Sbjct: 25 SSPAMLMLVLFLVIPLGIMFAVSIQQSGDYGGVKWGQHSLEAYLNFLWERDLDDSLVFNA 84 Query: 83 YYYGIFWRTLWISGLVTLICIVVGAPEAWILNRMRKPWRSIMLLVVLAPLMISVVVRAFG 142 Y GIF R+ W+S L TL C+ +G P A L + R+++L +V P +++VR + Sbjct: 85 DYLGIFQRSFWLSLLTTLGCLALGFPTALYLALQPERRRNLLLFLVTVPFWTNLLVRVYA 144 Query: 143 WSMLLGPEGLINGTLVALGFAK--VKLLYTETAIVIALVHVMLPFMVIPVWTSLQKLDGG 200 W +LL GL++ L +G + LLYT+ A++I L++ LPFMV+P+++SL+K+D Sbjct: 145 WILLLRNGGLVDAGLQGIGLTDTGLGLLYTDNAVIIGLLYTYLPFMVLPIYSSLEKMDWR 204 Query: 201 VANAALSLGASPITVLRRIVLPQIMPGILSGSLIVFGLSASSFAIPGLLGGRRLKMMATN 260 + AA LGA+ LRR+++P MPGI++G ++VF + S+ IP LLGG + M+ Sbjct: 205 LVEAAFDLGANRWKALRRVIVPLAMPGIVAGCILVFIPALGSYIIPELLGGGKALMIGNL 264 Query: 261 IH 262 I Sbjct: 265 IQ 266 >PM0263 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Pmu Pasteurella multocida Length = 284 Score = 144 bits (363), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 76/223 (34%), Positives = 139/223 (62%), Gaps = 4/223 (1%) Query: 45 LVPLVLTFVLSFNAYDAETGPKAGTFTLEHYTAVLTDPYYYGIFWRTLWISGLVTLICIV 104 L P +L ++SF D+ + A FTLE+YT L +P Y + W +L++SGL TL+C++ Sbjct: 23 LAPNLLVLIVSFLTRDS-SNFYALPFTLENYTR-LFEPLYAQVVWNSLYMSGLATLVCLI 80 Query: 105 VGAPEAWILNRMRKPWRSIMLLVVLAPLMISVVVRAFGWSMLLGPEGLINGTLVALGFAK 164 +G P A++L ++ +R +L +V+ P + ++R +G + LG +G++N TL+++G Sbjct: 81 IGYPFAFLLTKINPKYRPFLLFLVVLPFWTNSLIRIYGMKIFLGVKGILNSTLMSIGLID 140 Query: 165 --VKLLYTETAIVIALVHVMLPFMVIPVWTSLQKLDGGVANAALSLGASPITVLRRIVLP 222 +++L TE A++I LV+++LPFM++P++ S++KLD + AA LGA+ ++++P Sbjct: 141 TPIRILNTEVAVIIGLVYLLLPFMILPLYASIEKLDYRLLEAAKDLGANAFQRFIKVIIP 200 Query: 223 QIMPGILSGSLIVFGLSASSFAIPGLLGGRRLKMMATNIHGHH 265 MPGI++G L+V + F + LLGG ++ ++ I Sbjct: 201 LTMPGIIAGCLLVLLPAMGMFYVADLLGGAKVLLVGNVIKSEF 243 >CAC0839 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Cac Clostridium acetobutylicum Length = 277 Score = 139 bits (351), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 75/231 (32%), Positives = 133/231 (57%), Gaps = 8/231 (3%) Query: 35 PAILLCTVMLLVPLVLTFVLSFNAYDAETGPKAGTFTLEHYTAVLTDPYYYGIFWRTLWI 94 P L + +LVP+ L S + D+ TFTL +Y + Y +F+ ++ + Sbjct: 12 PYFLWSILFILVPIFLVIYFSLTSSDS-------TFTLNNYKKLFNSTYML-VFFNSIKL 63 Query: 95 SGLVTLICIVVGAPEAWILNRMRKPWRSIMLLVVLAPLMISVVVRAFGWSMLLGPEGLIN 154 + + T+IC +G P A+IL++ R+I++L ++ P+ ++ ++R + W +LG G+I+ Sbjct: 64 ALISTIICFFLGYPVAYILSKSSTKIRNILMLFLIIPMWMNFLLRTYAWMSILGRNGIIS 123 Query: 155 GTLVALGFAKVKLLYTETAIVIALVHVMLPFMVIPVWTSLQKLDGGVANAALSLGASPIT 214 L LGF + +LYT+ A+++ +V+ LPFM+IP++T L K+D V AA LGA+ Sbjct: 124 TILSFLGFKPLDILYTDAAVILGMVYNFLPFMIIPIYTVLIKIDRDVLKAASDLGANRFI 183 Query: 215 VLRRIVLPQIMPGILSGSLIVFGLSASSFAIPGLLGGRRLKMMATNIHGHH 265 + +RI+ P +PG++SG +VF + S+F I LLGG + ++ I Sbjct: 184 IFKRIIFPLSIPGVMSGVTMVFMPAVSTFVISKLLGGGQFMLIGNLIESQF 234 >mlr4191 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Mlo Mesorhizobium loti Length = 331 Score = 132 bits (332), Expect = 8e-31, Method: Compositional matrix adjust. Identities = 82/236 (34%), Positives = 132/236 (55%), Gaps = 4/236 (1%) Query: 31 LMSAPAILLCTVMLLVPLVLTFVLSFNAYDAETGPKAGTFTLEHYTAVLTDPYYYGIFWR 90 L+ APA++ T +++VP L L+F G TFTLE++ V DP Y IF + Sbjct: 50 LLMAPALVWLTALMVVPCALMLALAFFRRGIYGGIDY-TFTLENFGLVF-DPLYASIFLK 107 Query: 91 TLWISGLVTLICIVVGAPEAWILNRMRKPWRSIMLLVVLAPLMISVVVRAFGWSMLLGPE 150 + I+G TLI +V+G A+ ++ + W+ + L + P + ++R + W +LL E Sbjct: 108 SARIAGTATLIAVVIGYAAAYAISAAPRRWQPVFLFFAVLPFWSNYLIRTYAWIVLLNRE 167 Query: 151 GLINGTLVALGFAKV--KLLYTETAIVIALVHVMLPFMVIPVWTSLQKLDGGVANAALSL 208 GLI L G+ +LYTE A++ LV+ LPF+++ + L +L+ +A A+ L Sbjct: 168 GLITQLLRWSGYTGEPPSMLYTEGAVIAGLVYNYLPFVILACYAPLSRLNPELAEASRDL 227 Query: 209 GASPITVLRRIVLPQIMPGILSGSLIVFGLSASSFAIPGLLGGRRLKMMATNIHGH 264 GAS T RR++LP +PGI +G++ VF LS +F P LLGG R +M+ ++ Sbjct: 228 GASAATTFRRVILPLTVPGIAAGAVFVFVLSIGNFVTPALLGGGRFQMIGNLVYDQ 283