BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0204.p (379 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2825 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp ... 477 e-134 NMB0329 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp ... 456 e-128 NMA2159 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp ... 456 e-128 PA4526 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp p... 448 e-125 XF2544 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp p... 439 e-123 >RSc2825 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Rso Ralstonia solanacearum Length = 575 Score = 477 bits (1228), Expect = e-134, Method: Compositional matrix adjust. Identities = 229/400 (57%), Positives = 297/400 (74%), Gaps = 32/400 (8%) Query: 12 DDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLRDKLATR 71 DD PVVR L KL +A+ G SDLHFEPFE +RVR R+DG+L ++AQPPL +RDK+ATR Sbjct: 174 DDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATR 233 Query: 72 LKILARLDIAEKRLPQDGKMRLALGT--------------RTVDFRVSTLPTQFGEKIVL 117 +K+L+RLDI+EKR+PQDG+M+L + R +DFRVSTLPT FGEKIV+ Sbjct: 234 IKVLSRLDISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVI 293 Query: 118 RILDTAQAALSISDLGYEPAQQEALLQAISKPHGLVLMTGPTGSGKTVSLYACLQRLNQP 177 RIL+++ L I LGYEP Q++ LL I +P+G+VL+TGPTGSGKTVSLY L +LNQ Sbjct: 294 RILESSTERLDIDQLGYEPEQKQLLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNKLNQG 353 Query: 178 GVNIATAEDPVEINLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIA 237 +NI+TAEDP EI LPGINQV++N++AGL FA AL++FLRQDPD++MVGEIRDLETADI+ Sbjct: 354 DINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEIRDLETADIS 413 Query: 238 VKASQTGHLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLCR-CRRPA 296 +KA+QTGHLV STLHTNDAP TLTRL+NMGV +NIA+SV +I AQRL R+LC+ C++P Sbjct: 414 IKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPG 473 Query: 297 -----------------GGTPATFVPHGCPACNQTGFRGRTGIYQVMPVSAAMQSLILAQ 339 G+ + P GC ACN +G++GR GIYQVMP++ A+Q +ILA Sbjct: 474 PLPKETLLDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAH 533 Query: 340 AGTLELGRQAQAEGIIDLRRAGLLKVLRGETSVGEILACT 379 L++ QA+ EG++ LR +GL KV +G TS+ E+LA T Sbjct: 534 GTALQIAEQARKEGVLSLRDSGLRKVKQGLTSLEEVLATT 573 >NMB0329 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Nme Neisseria meningitidis MC58 Length = 558 Score = 456 bits (1172), Expect = e-128, Method: Compositional matrix adjust. Identities = 220/389 (56%), Positives = 296/389 (76%), Gaps = 21/389 (5%) Query: 12 DDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLRDKLATR 71 +D PV R +HK L+DA+ GASD+HFE +EH R+R R+DG L E+ QPP+++R +LA+R Sbjct: 168 EDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASR 227 Query: 72 LKILARLDIAEKRLPQDGKMRLAL--GTRTVDFRVSTLPTQFGEKIVLRILDTAQAALSI 129 +K+++RLDI+EKR+PQDG+M+L G + VDFRVSTLPT FGEK+V+RIL++ A+L+I Sbjct: 228 IKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNI 287 Query: 130 SDLGYEPAQQEALLQAISKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGVNIATAEDPVE 189 LG+EP Q++ LL+AI +P+G+VL+TGPTGSGKTVSLY CL LN VNIATAEDP E Sbjct: 288 DQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNTESVNIATAEDPAE 347 Query: 190 INLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTGHLVLS 249 INLPGINQV++N++ GL FA AL++FLRQDPD++MVGEIRDLETADIA+KA+QTGH+V S Sbjct: 348 INLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVFS 407 Query: 250 TLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLC-RCR----RPAGGT----- 299 TLHTN+APATL+R+LNMGV +NIA+SVSLI+AQRL+R+LC C+ RP+ Sbjct: 408 TLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVG 467 Query: 300 ---------PATFVPHGCPACNQTGFRGRTGIYQVMPVSAAMQSLILAQAGTLELGRQAQ 350 + GC C G++GR G+Y+VMP+S MQ +I+ +++ A Sbjct: 468 FTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAY 527 Query: 351 AEGIIDLRRAGLLKVLRGETSVGEILACT 379 EG++DLRRAG+LKV++G TS+ E+ A T Sbjct: 528 KEGMVDLRRAGILKVMQGITSLEEVTANT 556 >NMA2159 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB NmA Neisseria meningitidis Z2491 Length = 558 Score = 456 bits (1172), Expect = e-128, Method: Compositional matrix adjust. Identities = 220/389 (56%), Positives = 296/389 (76%), Gaps = 21/389 (5%) Query: 12 DDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLRDKLATR 71 +D PV R +HK L+DA+ GASD+HFE +EH R+R R+DG L E+ QPP+++R +LA+R Sbjct: 168 EDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASR 227 Query: 72 LKILARLDIAEKRLPQDGKMRLAL--GTRTVDFRVSTLPTQFGEKIVLRILDTAQAALSI 129 +K+++RLDI+EKR+PQDG+M+L G + VDFRVSTLPT FGEK+V+RIL++ A+L+I Sbjct: 228 IKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNI 287 Query: 130 SDLGYEPAQQEALLQAISKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGVNIATAEDPVE 189 LG+EP Q++ LL+AI +P+G+VL+TGPTGSGKTVSLY CL LN VNIATAEDP E Sbjct: 288 DQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNTESVNIATAEDPAE 347 Query: 190 INLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTGHLVLS 249 INLPGINQV++N++ GL FA AL++FLRQDPD++MVGEIRDLETADIA+KA+QTGH+V S Sbjct: 348 INLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVFS 407 Query: 250 TLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLC-RCR----RPAGGT----- 299 TLHTN+APATL+R+LNMGV +NIA+SVSLI+AQRL+R+LC C+ RP+ Sbjct: 408 TLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVG 467 Query: 300 ---------PATFVPHGCPACNQTGFRGRTGIYQVMPVSAAMQSLILAQAGTLELGRQAQ 350 + GC C G++GR G+Y+VMP+S MQ +I+ +++ A Sbjct: 468 FTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAY 527 Query: 351 AEGIIDLRRAGLLKVLRGETSVGEILACT 379 EG++DLRRAG+LKV++G TS+ E+ A T Sbjct: 528 KEGMVDLRRAGILKVMQGITSLEEVTANT 556 >PA4526 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Pae Pseudomonas aeruginosa Length = 566 Score = 448 bits (1152), Expect = e-125, Method: Compositional matrix adjust. Identities = 214/392 (54%), Positives = 292/392 (74%), Gaps = 19/392 (4%) Query: 6 DTAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLR 65 D + DD PVVR ++K+L DA+ G+SDLHFEP+E ++RVR R DG+LHE+A+PP+ L Sbjct: 174 DASAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLA 233 Query: 66 DKLATRLKILARLDIAEKRLPQDGKMRLALG-TRTVDFRVSTLPTQFGEKIVLRILDTAQ 124 +++ RLK++A LDI+E+R PQDG++++ + T+++DFRV+TLPT +GEKIV+RILD++ Sbjct: 234 SRISARLKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSS 293 Query: 125 AALSISDLGYEPAQQEALLQAISKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGVNIATA 184 A + I LGYE Q+E L A+ +P G++L+TGPTGSGKTVSLY L LN +NI+TA Sbjct: 294 AQMGIDALGYEEDQKELYLAALKQPQGMILVTGPTGSGKTVSLYTGLNILNTTDINISTA 353 Query: 185 EDPVEINLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTG 244 EDPVEINL GINQV++N R G+DF+ ALRAFLRQDPDV+MVGEIRDLETA+IA+KA+QTG Sbjct: 354 EDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLETAEIAIKAAQTG 413 Query: 245 HLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLC-RCRRPAG------ 297 H+V+STLHTN A TLTRLLNMGVPA+N+A SV+LI+AQRL RKLC C++ Sbjct: 414 HMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETL 473 Query: 298 ----------GTPATFVPHGCPACNQTGFRGRTGIYQVMPVSAAMQSLILAQAGTLELGR 347 GT + P GC C + G++GR GIY+V+ + A+Q +I+ + ++E+ Sbjct: 474 LHEGFPEDKIGTFKLYSPVGCDHC-KNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAE 532 Query: 348 QAQAEGIIDLRRAGLLKVLRGETSVGEILACT 379 QA+ EG DLR +GLLK ++G TS+ E+ T Sbjct: 533 QARKEGFNDLRTSGLLKAMQGITSLEEVNRVT 564 >XF2544 [NU] COG2804 Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB Xfa Xylella fastidiosa 9a5c Length = 577 Score = 439 bits (1129), Expect = e-123, Method: Compositional matrix adjust. Identities = 218/387 (56%), Positives = 284/387 (73%), Gaps = 20/387 (5%) Query: 12 DDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRLDGVLHELAQPPLSLRDKLATR 71 DD PVV+ ++K+L DA+ GASD+HFEP+E +RVR R+DG+L +A+ P L ++A R Sbjct: 190 DDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKGVAKAPNKLNQRIAAR 249 Query: 72 LKILARLDIAEKRLPQDGKMRLALG-TRTVDFRVSTLPTQFGEKIVLRILDTAQAALSIS 130 LK++++LDIAEKR+PQDG+++L + T+ VDFRVSTLPT FGEK+VLRILD + A L I Sbjct: 250 LKVMSQLDIAEKRVPQDGRIKLNVSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGID 309 Query: 131 DLGYEPAQQEALLQAISKPHGLVLMTGPTGSGKTVSLYACLQRLNQPGVNIATAEDPVEI 190 LGYE QQ ++AI KP+G+VL+TGPTGSGKTVSLY L LN NI+TAEDPVEI Sbjct: 310 KLGYEADQQTLFMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEI 369 Query: 191 NLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDLETADIAVKASQTGHLVLST 250 LPG+NQV N + G+ FA ALR+FLRQDPDV+MVGEIRDLETA+IA+KA+QTGH+VLST Sbjct: 370 RLPGVNQVQQNNKRGMTFAAALRSFLRQDPDVIMVGEIRDLETAEIAIKAAQTGHMVLST 429 Query: 251 LHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRKLC-RCRRPA----------GGT 299 LHTNDAP T+ RL+NMG+ YNI +SV+L++AQRL R+LC +C+RP G T Sbjct: 430 LHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPTNALLAEGFT 489 Query: 300 PAT-------FVPHGCPACNQTGFRGRTGIYQVMPVSAAMQSLILAQAGTLELGRQAQAE 352 + P GC C + G++GRTGIYQVMP++ + S+ILA L++ +AQ Sbjct: 490 QEQIDAGLELYEPVGCDECTE-GYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRI 548 Query: 353 GIIDLRRAGLLKVLRGETSVGEILACT 379 GI DLR++ LLKV +G T + EI T Sbjct: 549 GINDLRQSALLKVAQGITGLAEINRVT 575