BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0154.p (466 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value VC2031 [P] COG0659 Sulfate permease and related transporters (MF... 452 e-127 BH0414 [P] COG0659 Sulfate permease and related transporters (MF... 345 1e-94 PA2563 [P] COG0659 Sulfate permease and related transporters (MF... 339 8e-93 BS_ybaR [P] COG0659 Sulfate permease and related transporters (M... 332 2e-90 alr1633 [P] COG0659 Sulfate permease and related transporters (M... 328 2e-89 >VC2031 [P] COG0659 Sulfate permease and related transporters (MFS superfamily) Vch Vibrio cholerae Length = 521 Score = 452 bits (1164), Expect = e-127, Method: Compositional matrix adjust. Identities = 242/471 (51%), Positives = 324/471 (68%), Gaps = 10/471 (2%) Query: 1 VPECIAFALVAQLNPLMGLYGAFFICTITALFGGRPGMISGAAGSMAVVIVALVVQHGAQ 60 VPE +AFA VA ++P++GLY AF + IT++FGGRPGMISGA G+MAVV+V+LV HG Q Sbjct: 29 VPEAVAFAFVAGVDPMVGLYAAFIVGLITSIFGGRPGMISGATGAMAVVMVSLVASHGVQ 88 Query: 61 YFLATVVLSGVIMLLFGALRLGKLVRMVPHPVMLGFVNGLAIVIAMSQLEHFRVETPAGP 120 Y A ++L+G++ + G +LGK +RMVPHPVM+GFVNGLAIVI ++QL F+ G Sbjct: 89 YLFAAILLTGLLQVAAGLFKLGKFIRMVPHPVMIGFVNGLAIVIFLAQLGQFKAPDLNGV 148 Query: 121 Q-WLQGGALAIMIGLTLATMLIVWLLPRVTKAIPPALAAILGVGLVTYFSGLHTRTLADM 179 WL L +M+GL TM I+ LP++T AIP +LAAI+ V L+ GL TRT+ D Sbjct: 149 MTWLPQKELLLMLGLVALTMAIIHFLPKLTTAIPSSLAAIVTVTLLVQGLGLETRTVVDF 208 Query: 180 ---------AQIAGGLPPLHWPSVPMNLETLRIIGPYAVLMAIVGLLETLLTFNLTDEIT 230 A +AG LP P+VP+ ETL+II PYA+++A +GL+E+LLT + DE+T Sbjct: 209 LRAMSGNDAATLAGSLPSFSIPAVPLTWETLKIIFPYALILAAIGLIESLLTLTVLDEMT 268 Query: 231 ASRGQPNRECLALGAANIVSGLFGGMGGCAMIGQTMINLGSGGRSRLSGVVAGGMILLFI 290 +RGQ NREC+ G ANI +FG MGGCAMIGQ+MIN+ SGGR RLSG+VA ++L FI Sbjct: 269 GTRGQSNRECMGQGLANITCSMFGAMGGCAMIGQSMINVNSGGRGRLSGIVAAVLLLCFI 328 Query: 291 LFLSPAIERIPLAALAGVMFVVAQETFAWGSLRVLGKVPRSDALVIIAVTIITVLTDLAV 350 LF + IE IPLAAL GVMF+V TF W + ++ +VP+ D VI+ VT++TV TDLA+ Sbjct: 329 LFTASLIEMIPLAALVGVMFMVVIGTFEWATFKLARRVPKQDFFVIVLVTVVTVFTDLAI 388 Query: 351 AVLCGIVITALNFAWQHAREIRADRRDEADGSRTYLPQGTLFFASTTHFLDLFDTANDPQ 410 AV G++ +AL FAW+HA+ I A DG + Y G +FF S +FL+LFD +DP+ Sbjct: 389 AVAVGVIASALMFAWEHAKHIYAASHINPDGHKEYHIHGPIFFGSAANFLELFDAHHDPK 448 Query: 411 QVTLDCRHLRLADHSAIAAVQTLAERYVRAGKELRVVALSQRCSQLLERAG 461 +V +D R+ADHSAI A++TLAERY GK L + LSQ C LL++ G Sbjct: 449 EVIVDFARSRVADHSAIDAIETLAERYAAVGKTLHLRHLSQDCRALLQKVG 499 >BH0414 [P] COG0659 Sulfate permease and related transporters (MFS superfamily) Bha Bacillus halodurans Length = 486 Score = 345 bits (884), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 191/462 (41%), Positives = 282/462 (61%), Gaps = 13/462 (2%) Query: 1 VPECIAFALVAQLNPLMGLYGAFFICTITALFGGRPGMISGAAGSMAVVIVALVVQHGAQ 60 +PE IAF+++A ++P++GLY +F I + A GGRPGMIS A G+MA++++ LV HG Sbjct: 29 IPEAIAFSIIAGVDPMVGLYASFCIAVVIAFVGGRPGMISAATGAMALLMINLVADHGLH 88 Query: 61 YFLATVVLSGVIMLLFGALRLGKLVRMVPHPVMLGFVNGLAIVIAMSQLEHFRVETPAGP 120 Y LA +L+GV+ +LFG +L +++ VP VM+GFVN LAI+I SQL+HF ET Sbjct: 89 YLLAATILTGVLQILFGVFKLASVMKFVPRSVMIGFVNALAILIFTSQLQHFVGET---- 144 Query: 121 QWLQGGALAIMIGLTLATMLIVWLLPRVTKAIPPALAAILGVGLVTYFSGLHTRTLADMA 180 W +M L T+ I++LLP+VT A+P L AI+ V + F + RT+ D+ Sbjct: 145 -W-------VMYALVALTLAIIYLLPKVTTAVPSTLVAIIIVTAIVIFGNIGVRTVGDLG 196 Query: 181 QIAGGLPPLHWPSVPMNLETLRIIGPYAVLMAIVGLLETLLTFNLTDEITASRGQPNREC 240 + LP PSVP + ETL II PY++ +AIVGLLE+LLT N+ D++T + N+E Sbjct: 197 TLTQSLPTFLIPSVPFSFETLMIIFPYSLALAIVGLLESLLTANIVDDMTDTESDKNKES 256 Query: 241 LALGAANIVSGLFGGMGGCAMIGQTMINLGSGGRSRLSGVVAGGMILLFILFLSPAIERI 300 G ANIV+G FGGM GCAMIGQ++IN+ SGGR RLS +VAG ++ I+ L + +I Sbjct: 257 RGQGIANIVTGFFGGMAGCAMIGQSVINVKSGGRGRLSALVAGVFLMFLIIVLGNVVVQI 316 Query: 301 PLAALAGVMFVVAQETFAWGSLRVLGKVPRSDALVIIAVTIITVLT-DLAVAVLCGIVIT 359 P+AALAGVM +V+ TF W SLR L K+PR+DA V++ V T DL+ VL G+V++ Sbjct: 317 PMAALAGVMIMVSIGTFDWSSLRNLHKIPRTDATVMVVTVGTVVYTHDLSKGVLAGVVLS 376 Query: 360 ALNFAWQHAREIRADRRDEADGSRTYLPQGTLFFASTTHFLDLFDTANDPQQVTLDCRHL 419 A+ FA + +R + + Y QG LFFAS + F + +V +D Sbjct: 377 AIFFAAKISRVKVEEMTSATPELKRYKVQGQLFFASVSDLPAKFSYMANVNEVEIDLSEA 436 Query: 420 RLADHSAIAAVQTLAERYVRAGKELRVVALSQRCSQLLERAG 461 + D SA+ A+ + ++ + ++ L+ +L++R G Sbjct: 437 HVWDDSAVGALDKIEMKFEQQNIKVTFTGLNTESRKLVQRMG 478 >PA2563 [P] COG0659 Sulfate permease and related transporters (MFS superfamily) Pae Pseudomonas aeruginosa Length = 495 Score = 339 bits (870), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 196/465 (42%), Positives = 285/465 (61%), Gaps = 15/465 (3%) Query: 1 VPECIAFALVAQLNPLMGLYGAFFICTITALFGGRPGMISGAAGSMAVVIVALVVQHGAQ 60 +PE IAF+++A ++P +GLY +F + + A GGRPGMIS A G+MA+++V LV HG Q Sbjct: 28 IPEAIAFSIIAGVDPKIGLYASFSMAVVIAFAGGRPGMISAATGAMALLMVNLVKDHGLQ 87 Query: 61 YFLATVVLSGVIMLLFGALRLGKLVRMVPHPVMLGFVNGLAIVIAMSQLEHFRVETPAG- 119 Y LA +L GV+ +L G LRLG L+R V V+ GFVN LAI+I M+QL T G Sbjct: 88 YLLAATLLCGVLQILAGYLRLGSLMRFVSRSVVTGFVNALAILIFMAQLPEL---TDVGW 144 Query: 120 PQW-LQGGALAIMIGLTLATMLIVWLLPRVTKAIPPALAAILGVGLVTYFSGLHTRTLAD 178 P + + LAI+ G LPR+ + +P L IL + ++ + GL RT+ D Sbjct: 145 PVYAMTAAGLAIVYGFPH--------LPRIGRLLPSPLVCILVLTGISIYLGLDIRTVGD 196 Query: 179 MAQIAGGLPPLHWPSVPMNLETLRIIGPYAVLMAIVGLLETLLTFNLTDEITASRGQPNR 238 M Q+ LP WP VP+NL+TL I+ PY+ +A+VGLLE+L+T + DE+T + NR Sbjct: 197 MGQLPDSLPLFLWPDVPLNLDTLLIVLPYSAALAVVGLLESLMTATIVDEMTDTGSDKNR 256 Query: 239 ECLALGAANIVSGLFGGMGGCAMIGQTMINLGSGGRSRLSGVVAGGMILLFILFLSPAIE 298 EC G ANI +GL GGM GCAMIGQ++IN+ SGGRSRLS +VAG +LL ++FLS + Sbjct: 257 ECKGQGLANIATGLLGGMAGCAMIGQSVINVKSGGRSRLSTLVAGVALLLMVVFLSDWVG 316 Query: 299 RIPLAALAGVMFVVAQETFAWGSLRVLGKVPRSDALV-IIAVTIITVLTDLAVAVLCGIV 357 RIP+AAL VM +V+ TF W S+R L + P S LV ++ V ++ +LA V+ G++ Sbjct: 317 RIPMAALVAVMIMVSIGTFRWESIRNLKRYPLSTNLVMLVTVGVVLATHNLAFGVVAGVL 376 Query: 358 ITALNFAWQHAREIRADRRDEADGSRT-YLPQGTLFFASTTHFLDLFDTANDPQQVTLDC 416 + +L FA + + D ADG+R Y G +FFAS+ F+ FD + V +D Sbjct: 377 LASLFFANKIGHYLDIDSERSADGTRRHYQVSGQVFFASSGRFVAAFDLKETLEHVLIDL 436 Query: 417 RHLRLADHSAIAAVQTLAERYVRAGKELRVVALSQRCSQLLERAG 461 RH L D +A+AA+ + + + G ++ V+ L+ + L++R G Sbjct: 437 RHAHLWDVTAVAALDKVVLGFRQRGVQVDVLGLNAASATLVDRFG 481 >BS_ybaR [P] COG0659 Sulfate permease and related transporters (MFS superfamily) Bsu Bacillus subtilis Length = 478 Score = 332 bits (850), Expect = 2e-90, Method: Compositional matrix adjust. Identities = 178/460 (38%), Positives = 275/460 (59%), Gaps = 14/460 (3%) Query: 1 VPECIAFALVAQLNPLMGLYGAFFICTITALFGGRPGMISGAAGSMAVVIVALVVQHGAQ 60 +PE I F+++A ++P++GLY +F I I ++FGGRPGMIS A GSMAVV+V+LV HG Q Sbjct: 30 IPEAIGFSIIAGVDPMVGLYASFCIAIIISIFGGRPGMISAATGSMAVVMVSLVADHGLQ 89 Query: 61 YFLATVVLSGVIMLLFGALRLGKLVRMVPHPVMLGFVNGLAIVIAMSQLEHFRVETPAGP 120 Y A +L+G+I ++ G ++ +L++ +P VM+GFVN LAI+I +QL F G Sbjct: 90 YLFAATILTGIIQVILGISKIARLMKFIPRSVMIGFVNALAILIFSAQLPQFE-----GA 144 Query: 121 QWLQGGALAIMIGLTLATMLIVWLLPRVTKAIPPALAAILGVGLVTYFSGLHTRTLADMA 180 W LA +++I+++LPR T A+P L AI+ + ++ + RT+ DM Sbjct: 145 SWSMYAMLA-------GSLVIIYVLPRFTTAVPSPLVAIIVMTIIAVTFHVDVRTVGDMG 197 Query: 181 QIAGGLPPLHWPSVPMNLETLRIIGPYAVLMAIVGLLETLLTFNLTDEITASRGQPNREC 240 I+ LP P VP ETL+II PY++ +A VGLLE+LLT + DE+T + N+E Sbjct: 198 NISSSLPHFLIPDVPFTFETLQIIFPYSIALAFVGLLESLLTAQIIDEMTDTDSDKNKES 257 Query: 241 LALGAANIVSGLFGGMGGCAMIGQTMINLGSGGRSRLSGVVAGGMILLFILFLSPAIERI 300 G ANIV+G FGGM GCAMIGQ++IN +GGR RLS VAG ++ I LS + +I Sbjct: 258 RGQGIANIVTGFFGGMAGCAMIGQSVINTKAGGRGRLSAFVAGAFLMFLIAVLSHVVVKI 317 Query: 301 PLAALAGVMFVVAQETFAWGSLRVLGKVPRSDAL-VIIAVTIITVLTDLAVAVLCGIVIT 359 P+AAL VM +V+ TF W SL+ L K P +D++ +++ V + V DL+ V G++++ Sbjct: 318 PMAALVAVMVMVSVGTFDWSSLKGLKKAPLTDSIVMVVTVVTVVVTDDLSKGVFVGVLLS 377 Query: 360 ALNFAWQHAREIRADRRDEADGSRTYLPQGTLFFASTTHFLDLFDTANDPQQVTLDCRHL 419 A+ F + ++ ++ E RTY +G +FFAS T + F D ++V +D Sbjct: 378 AVFFVAKISK-LKIVSHAEDQKLRTYQVKGQIFFASVTDLTNAFIYQEDIERVVIDLTEA 436 Query: 420 RLADHSAIAAVQTLAERYVRAGKELRVVALSQRCSQLLER 459 + D S AA+ + ++ G E + L++ L+++ Sbjct: 437 HVWDDSGAAALDKIVAKFKEQGIEAELKGLNKASKSLMKQ 476 >alr1633 [P] COG0659 Sulfate permease and related transporters (MFS superfamily) Nos Nostoc sp. PCC 7120 Length = 472 Score = 328 bits (841), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 177/466 (37%), Positives = 278/466 (59%), Gaps = 14/466 (3%) Query: 1 VPECIAFALVAQLNPLMGLYGAFFICTITALFGGRPGMISGAAGSMAVVIVALVVQHGAQ 60 +PE IAF+++A ++P +GLY +F I +TA GGRPG IS A G+MA++++ LV HG Q Sbjct: 11 IPEAIAFSIIAGVDPKVGLYASFIIAVMTAFLGGRPGSISAATGAMALLMIDLVKDHGLQ 70 Query: 61 YFLATVVLSGVIMLLFGALRLGKLVRMVPHPVMLGFVNGLAIVIAMSQLEHFRVETPAGP 120 Y A +L+GV ++FG L+LG+ +R VP VM+G++N LA++I ++QL P Sbjct: 71 YLFAATLLTGVFQVVFGFLQLGRQMRFVPRAVMIGYINALAVLIFLAQLPQLTNVPPT-- 128 Query: 121 QWLQGGALAIMIGLTLATMLIVWLLPRVTKAIPPALAAILGVGLVTYFSGLHTRTLADMA 180 + LTL ++ I+++LPR TKA+P L A+ + + L + DM Sbjct: 129 ----------VYILTLLSLGIIYILPRFTKAVPSPLVALAVMTIAAIALKLKVPRVGDMG 178 Query: 181 QIAGGLPPLHWPSVPMNLETLRIIGPYAVLMAIVGLLETLLTFNLTDEITASRGQPNREC 240 ++ LP P VP+ LET +II PY++ +AIVGLL + LT +L DE+T + N+E Sbjct: 179 ELPTALPSFALPQVPLTLETFKIILPYSLTLAIVGLLASFLTASLVDELTDTPSDKNQEA 238 Query: 241 LALGAANIVSGLFGGMGGCAMIGQTMINLGSGGRSRLSGVVAGGMILLFILFLSPAIERI 300 G ANIV+ FGGM GC MIGQ++IN+ SGGR RLS + AG +L+ ILFL ++++ Sbjct: 239 KGQGIANIVTAFFGGMAGCGMIGQSVINVQSGGRGRLSTLAAGVFLLIAILFLQDWVKQM 298 Query: 301 PLAALAGVMFVVAQETFAWGSLRVLGKVPRSDALVIIAVTIITVLT-DLAVAVLCGIVIT 359 P+AAL VM +V+ TF W S + + ++PR++ V++ +T+ T + A+ V+ GIV++ Sbjct: 299 PMAALVAVMIMVSIGTFRWTSFKNISRIPRTETAVMLTTMFVTIFTRNFALGVVTGIVMS 358 Query: 360 ALNFAWQHAREIRADRRDEADGS-RTYLPQGTLFFASTTHFLDLFDTANDPQQVTLDCRH 418 + F+ + A+ + D+ DG+ R Y G +FF S FL FD ++VT+D H Sbjct: 359 TVFFSNKIAQLVFVDKVLSEDGTHRIYKVVGQIFFLSRDEFLGFFDFTELLERVTIDLTH 418 Query: 419 LRLADHSAIAAVQTLAERYVRAGKELRVVALSQRCSQLLERAGLPQ 464 L D A+ + ++ R G E+ ++ L+Q + LL + Q Sbjct: 419 AHLWDQGAVEVLDRAVLKFRRNGAEVELIGLNQASATLLNKLATHQ 464